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Sains Natural: Journal of Biology and Chemistry
Published by Universitas Nusa Bangsa
ISSN : 20863446     EISSN : 2621508X     DOI : https://doi.org/10.31938/jsn
Core Subject : Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry
Jurnal Sains Natural is a peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication with the objectives to explore the knowledge about natural sciences. This journal incorporates not only all branches of chemistry and biology, but also sub-disciplines like Biochemistry, Polymer, Agricultural chemistry, Environmental chemistry, etc.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 5 Documents
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Search results for , issue "Vol. 8 No. 1 (2018): Sains Natural" : 5 Documents clear
ISOLASI DAN KARAKTERISASI KOLAGEN DARI KULIT IKAN PATIN (Pangasius sp.) Pipih Suptijah; Dini Indriani; Supriyono Eko Wardoyo
JURNAL SAINS NATURAL Vol. 8 No. 1 (2018): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (550.85 KB) | DOI: 10.31938/jsn.v8i1.106

Abstract

Isolation and Characterization of Collagen from the Skin of Catfish (Pangasius sp.)           Skin of catfish is one of aquatic by-products which could be used as an alternative source of collagen. This research is aimed to isolate and characterize collagen from skin of catfish. Methods of  isolation of collagen included three stages, the first was deproteinization using NaOH solution with concentration of 0.05 M; 0.10 M; 0.15 M; 0.20 M for 12 hours, the second was soaking in CH3COOH solution with concentration of 0.05 M; 0.10 M; 0.15 M; and 0.20 M for 2 hours, and the third was extraction in water at a temperature of 40 0C for 2 hours; characterization of collagen was included chemical and physical properties. The results showed that the best extraction method ofcollagen from skin of catfish was soaking the skin in 0.05 M NaOH solution for 12 hours and soaking the skin in 0.05 M acetic acid for 2 hours. Extraction yields of collagen was 12.15%. Chemical characteristics included proximate and amino acid composition. Proximate value of collagen consisted of moisture was 6.55%, ash 1.80%,  protein 64.74% and fat 8.85%.  The major amino acid composition of collagen were glycine, proline, alanine, arginine and glutamate. Physical characteristics of collagen resulted from FTIR analysis showed amide A, amide B, amide I, amide II and amide III, triple helical structure of the amide I and amide III indicates that the compound produced was collagen; color analysis was 66.39%; thermal analysis showed a melting temperature peak was 154.47 0C and pH value was 5.34.Keywords : Catfish, isolation, characterization, collagen, skin ABSTRAK          Kulit ikan patin merupakan salah satu limbah hasil perairan yang dapat digunakan sebagai sumber alternatif kolagen. Penelitian ini bertujuan untuk mengisolasi dan karakterisasi kolagen yang diperoleh dari kulit ikan patin. Isolasi kolagen yang dilakukan meliputi tiga tahap, yaitu tahap pertama adalah proses deproteinisasi menggunakan larutan NaOH dengan konsentrasi, yaitu 0,05 M; 0,10 M; 0,15 M; 0,20 M dan lama waktu perendaman selama 12 jam; tahap kedua, yaitu perendaman dalam larutan CH3COOH dengan empat konsentrasi CH3COOH yaitu 0,05 M; 0,10 M; 0,15 M; dan 0,20 M dan lama waktu perendaman selama 2 jam; dan tahap ketiga, yaitu ekstraksi dengan air pada suhu 40 0C selama 2 jam; serta karakterisasi kolagen yang dilakukan, meliputi sifat kimia dan fisik. Hasil penelitian menunjukkan bahwa metode ekstraksi kolagen dari kulit ikan patin  terbaik diperoleh melalui proses perendaman kulit dalam larutan NaOH 0,05 M selama 12 jam dan  perendaman kulit dalam asam asetat 0,05 M selama 2 jam.  Rendemen serbuk kolagen yang dihasilkan sebesar 12,15 %. Karakteristik kimia meliputi proksimat dan komposisi asam amino. Nilai proksimat kolagen terdiri dari kadar air 6,55 %,  abu 1,80 %, protein 64,74 % dan lemak 8,85 %. Komposisi asam amino yang dominan pada kolagen adalah glisina, prolina, alanina, arginina dan glutamat. Karakteristik fisik kolagen yang dihasilkan adalah analisis FTIR menunjukkan adanya gugus amida A, amida B, amida I, amida II dan amida III, struktur triple heliks pada amida I dan amida III mengindikasikan bahwa senyawa yang dihasilkan adalah kolagen; analisis warna  yaitu 66,39 %; analisis termal yang menunjukkan suhu puncak pelelehan adalah 154,47 0C dan nilai pH kolagen yaitu 5,34. Kata kunci : Ikan patin, isolasi, karakterisasi, kolagen, kulit 
STUDI DINAMIKA SENYAWA FOSFAT DALAM KUALITAS AIR SUNGAI CILIWUNG HULU KOTA BOGOR RTM Sutamihardja; Mia Azizah; Yunita Hardini
JURNAL SAINS NATURAL Vol. 8 No. 1 (2018): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (392.301 KB) | DOI: 10.31938/jsn.v8i1.114

Abstract

Study of Dynamics of Phosphate in the Water Quality in Bogor of Upstream Ciliwung RiverCiliwung River has existed and become an important part of the community since ancient period. Since 2009, the Ciliwung River has been polluted condition from upstream. One of the pollutant that could decrease the quality of river water was phosphate. Excessive phosphate level in water bodies caused nutrient enrichment conditions (eutrophication). The presence of nitrate supporting phosphate also caused algae blooming, one of the environmental problem. The research was conducted to determine the dynamics of the phosphate compound of Ciliwung River whether the pollution was reduced, same, or worse. The research included sampling of river water at three points of Katulampa, Pasar Bogor, and Warung Jambu River with sampling time interval of 8 hours in a day for 3 weeks in a row. The data of phosphate and nitrate concentration were measured and compared to the results in PP No.82 year 2001. The total of phosphate in Ciliwung river water has exceeded threshold level in accordance with the environmental quality standard of PP. 82 year 2001, and has been indicated to be in eutrophication condition.Keywords: Ciliwung River, Water Quality, Water Pollution, Phosphate.ABSTRAKSungai Ciliwung telah ada dan menjadi bagian penting masyarakat sejak zaman purba. Namun seiring dengan berlalunya waktu dan perkembangan pesat, sejak tahun 2009 Sungai Ciliwung telah tercemar dari hulu. Salah satu polutan yang bisa menurunkan kualitas air sungai adalah fosfat. Keberadaan fosfat yang berlebihan di badan air dapat menyebabkan kondisi pengayaan nutrisi (eutrofikasi), dan dengan dukungan nitrat dapat menyebabkan algae blooming yang menjadi salah satu masalah lingkungan. Penelitian ini dilakukan untuk mengetahui dinamika senyawa fosfat Sungai Ciliwung apakah pencemarannya berkurang, sama, atau lebih buruk. Ruang lingkup penelitian meliputi pengambilan sampel air sungai pada tiga titik Katulampa, Pasar Bogor, dan Warung Jambu dengan interval waktu sampling 8 jam dalam sehari, dan dilakukan setiap minggu selama 3 minggu berturut-turut. Kemudian dilakukan analisis sampel di laboratorium, serta interpretasi data dengan membandingkan hasilnya terhadap PP No.82 tahun 2001. Nilai total fosfat di air sungai Ciliwung Hulu tidak memenuhi standar kualitas lingkungan PP. 82 tahun 2001, dan diindikasikan berada dalam kondisi eutrofikasi.Kata kunci: Sungai Ciliwung, Kualitas Air, Polusi Air, Fosfat.
PARAMETER FISIKA DAN KIMIA AIR KOLAM IKAN NILA HITAM (Oreochromis niloticus) Meilinda Pramleonita; Nia Yuliani; Ridha Arizal; Supriyono Eko Wardoyo
JURNAL SAINS NATURAL Vol. 8 No. 1 (2018): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (664.032 KB) | DOI: 10.31938/jsn.v8i1.107

Abstract

Physical and Chemical Parameters of Water Fish Pond Black Nile Tilapia (Oreochromis niloticus) Water is a natural resource that is essential for the survival of humans and other creatures, role of water is essential for life on earth, especially fish in the water habitat. Fish need a comfortable environment in order to be healthy and growing optimally. Therefore, the water, used as a habitat of life for the fish, have certain requirements. So the quality of the water must be very noted. Tilapia is a freshwater fish that has a great tolerance towards the environment, therefore it is highly appreciated by fish farmers in Indonesia.  Study of physical and chemical parameters in water of  tilapia fish pond was done due to lack of review of water quality of tilapia fish pond. The review is based on a sampling of water, i.e. morning and afternoon. A review of the pond water quality was expected to assist fish farmers got information about the qualities of water of tilapia fish pond in physical and chemical characteristics.  The method of this research was Grab (momentarily) methods in water sampling. Testing of water samples in physics done visually for color parameters, temperature using a thermometer device, and brightness parameters was using the secchi disk. In testing the chemical parameters were measurement of pH was using a pH meter Winkler method was done  for the parameters of dissolved oxygen (DO). Titrimetric method was done  for the parameters of  total hardness, and  carbon dioxide, whereas for ammonia parameter was done  by spectrophotometric method were measured using UV-Vissible. After testing all the parameters then interpretation was done for the parameter data.  The results showed the water quality of tilapia fish pond in the area Laladon – Bogor was not  yet qualified for a good fish pond water based on ISO 7550: 2009 Product ion of tilapia growing  level in calm water pond,  for parameter Ammonia levels should be <0.02 mg / L, and based on PPNo.8, on water Quality Standard by 2001. The difference in the parameter levels in the morning and during the day due to the process of respiration at night by the aquatic organisms that produce CO2 gas and the process of photosynthesis during the day by plankton, microalgae, and other aquatic plants to produce a compound O2. The existence of other human activities during the day also affects the difference in the data levels in the morning and day time.Keywords: Tilapia, pond water qualityABSTRAK Air merupakan sumber daya alam yang sangat penting bagi kelangsungan hidup manusia dan makhluk lainnya, Peranan air sangat penting bagi makhluk hidup di bumi terutama ikan yang berhabitat di dalam air. Ikan membutuhkan lingkungan yang nyaman agar dapat hidup sehat dan tumbuh secara optimal. Oleh karena itu  air yang digunakan sebagai sumber kehidupan bagi ikan, memiliki persyaratan tertentu. Sehingga kualitas dari air harus sangat di perhatikan. Ikan nila merupakan ikan air tawar yang memiliki nilai toleransi yang besar terhadap lingkungannya sehingga sangat diminati oleh petani ikan di Indonesia. Studi parameter fisika dan kimia pada air kolam ikan nila dilakukan karena kurangnya peninjauan terhadap kualitas air kolam ikan nila. Peninjauan tersebut dilakukan berdasarkan waktu pengambilan sampel air, yaitu pada pagi dan siang hari. Peninjauan kualitas air kolam diharapkan dapat membantu para petani ikan mendapatkan informasi mengenai kelayakan kolam ikan nila secara fisika dan kimia.  Metode yang dilakukan penelitian ini adalah metode Grab (sesaat) untuk pengambilan sampel air. Pengujian sampel air secara fisika dilakukan secara visual untuk parameter warna, suhu dengan menggunakan alat pengukur suhu, dan metode secchi disk untuk parameter kecerahan. Pada pengujian parameter kimia dilakukan pengukuran pH dengan alat pengukur pH. Metode Winkler dilakukan untuk parameter dissolved oxygen (DO). Metode titrimetrik dilakukan untuk parameter s kesadahan total, dan karbondioksida, sedangkan untuk parameter ammonia dilakukan dengan metode spektrofotometri yang diukur dengan menggunakan alat spektrofotometer UV-Vissible. Setelah dilakukan pengujian semua parameter maka dilakukan interpretasi data hasil penelitian.  Hasil penelitian menunjukan kualitas air kolam ikan nila di daerah Laladon – Bogor belum memenuhi syarat untuk air kolam ikan yang baik berdasarkan SNI 7550:2009 Produksi ikan nila tingkat  pembesaran di kolam air tenang, untuk parameter Ammonia dengan kadar <0,02 mg/L, dan  berdasarkan Baku Mutu PPNo.82 tahun 2001. Terjadinya perbedaan kadar pada pagi dan siang hari dikarenakan terjadinya proses respirasi pada malam hari oleh organisme air sehingga menghasilkan senyawa CO2 dan terjadinya proses fotosintesis pada siang hari oleh plankton, mikroalga, dan tanaman air lainnya sehingga menghasilkan senyawa O2. Adanya aktifitas lain pada siang hari juga mempengaruhi terjadinya perbedaan kadar pada pagi dan siang hari.Kata kunci : Ikan nila, Kualitas air kolam
KANDUNGAN FITOKIMIA DAN SENYAWA KATINON PADA DAUN KHAT MERAH (Catha edulis) Mimi Adhariani; Mamay Maslahat; RTM Sutamihardja
JURNAL SAINS NATURAL Vol. 8 No. 1 (2018): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (395.476 KB) | DOI: 10.31938/jsn.v8i1.113

Abstract

Phytochemical Content and Katinon Coumpound in Red Khat (Catha edulis) Leaves          Khat Plant (Catha edulis) belongs to the Celastraceae family that originated in East Africa and the Arabian plateau. Khat plant has green leaves with finely toothed oval shape resembling betel leaves and fragrant. The active component Khat leaves was cathinone which is an alkaloids group. Cathinone which has the chemical formula of 2-amino-1-phenyl propanone is often referred to as a natural amphetamine because it produced effects like amphetamine that could penetrate the nervous system , adrenaline and as stimulant. The purpose of this study was to identify phytochemical compounds in red khat leaves (Catha edulis) using gas chromatography-mass spectra. The results showed that extracts of red leaf khat (Catha edulis) contained of secondary metabolites, they were alkaloids, phenolics, glycosides, steroids / triterpenoids, flavonoids, tannins and saponins. Based on the identification of khat leaf extract (Catha edulis) at pH 8, 9 and 10 showed the presence of katin compounds at different concentrations, while the cathinone compounds were not identified at all three types of the extract.Keywords : Catha edulis, phytochemical ,metabolite secondary, gas chromatography-mass spectra ABSTRAK          Tanaman Khat (Catha edulis) termasuk dalam famili Celastraceae yang berasal dari Afrika Timur dan dataran Arab. Tanaman Khat memiliki daun berwarna hijau bergerigi halus dengan bentuk oval menyerupai daun sirih, dan berbau harum.  Komponen aktif daun Khat yaitu katinona merupakan senyawa kimia golongan  alkaloid. Katinona dengan rumus kimia 2-amino-1-fenil propanon sering disebut sebagai amfetamin alami  karena menghasilkan efek seperti amfetamin yang bisa menembus susunan saraf pusat, memacu adrenalin dan stimulansia. Tujuan penelitian ini untuk mengidentifikasi kandungan senyawa fitokimia dan katinon dalam daun khat merah (Catha edulis) menggunakan kromatografi gas spektra massa .Hasil penelitian menunjukan serbuk simplisia daun khat merah (Catha edulis) mengandung senyawa metabolit sekunder yaitu alkaloid, fenolik, glikosida, steroid/triterpenoid, flavonoid, tanin serta saponin. Berdasarkan identifikasi ekstrak daun khat merah (Catha edulis) pada pH 8, 9 dan 10 menggunakan KG-SM menunjukan adanya senyawa katin pada konsentrasi yang berbeda, sedangkan senyawa katinona tidak ditemukan pada ke tiga jenis pH ekstrak tersebut.Kata kunci : Catha edulis, fitokimia, metabolit sekunder, kromatografi gas-spektra massa
RITME HARIAN KONSUMSI OKSIGEN INDUK IKAN MAS Cyprinus carpio DENGAN FOTOPERIODE KONTINYU 24 JAM Vitas Atmadi Prakoso; Jun Hyung Ryu; Young Jin Chang
JURNAL SAINS NATURAL Vol. 8 No. 1 (2018): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.531 KB) | DOI: 10.31938/jsn.v8i1.105

Abstract

DIEL RHYTHM OF OXYGEN CONSUMPTION ON ADULT COMMON CARP Cyprinus carpio WITH CONTINUOUS 24-HOURS PHOTOPERIOD         Some external factors could affect the metabolism rate of fish. One of them is photoperiod which related to the length of day and night in a day. Based on that correlation, the experiment was conducted to determine the effects of photoperiod manipulation to the diel rhythm of oxygen consumption on adult common carp Cyprinus carpio. The experimental fish (total length: 29.2 ± 0.4 cm, total weight: 1295.8 ± 56.1 g) was observed in respirometer which connected in a closed recirculation system. The study was conducted with three replications with one individu in each replication. The water temperature and closed recirculation system was maintained at 20.2 ± 0.3oC. The photoperiod was set to 24 hours light : 0 hours of dark by using fluorescent lamp which lit continuously. The results showed that during 24 hours observation at 24 hours light: 0 hours dark condition, adult common carp has an oxygen consumption range of 24.5 - 29.1 mg O2/kg/h at 20oC. The average value of the lowest oxygen consumption occurred at 02.00 and 17.00 (24.5 ± 1.9 mg O2/kg/h). Meanwhile, the highest oxygen consumption value was recorded at 06.00 (29.1 ± 1.7 mg O2/kg/h). Based on data of oxygen consumption obtained from this study, no significant difference found between oxygen consumption of adult common carp during day and night with photoperiod manipulation. The activity and metabolism of adult common carp have changed with the change of photoperiod.Keywords: Common carp, Cyprinus carpio, oxygen consumption, photoperiod. ABSTRAK          Beberapa faktor eksternal dapat mempengaruhi laju metabolisme pada ikan. Salah satu diantaranya adalah fotoperiode yang berkaitan dengan lamanya siang dan malam dalam satu hari. Berdasarkan korelasi tersebut, maka dilakukan penelitian untuk mengetahui pengaruh manipulasi fotoperiode terhadap ritme harian konsumsi oksigen pada induk ikan mas Cyprinus carpio. Ikan uji (panjang total 29,2 ± 0,4 cm; bobot total 1295,8 ± 56,1 g) diamati di dalam respirometer yang terhubung dalam sistem resirkulasi tertutup. Penelitian dilakukan dengan tiga ulangan dengan masing-masing jumlah induk ikan sebanyak satu ekor. Suhu air dan sistem resirkulasi tertutup diatur pada suhu 20,2 ± 0,3oC. Fotoperiode diatur pada kondisi 24 jam terang : 0 jam gelap dengan menggunakan lampu fluorescent yang dinyalakan secara kontinyu. Hasil penelitian menunjukkan bahwa selama pengamatan 24 jam kondisi 24 jam terang : 0 jam gelap, induk ikan mas memiliki kisaran konsumsi oksigen sebesar 24,5 - 29,1 mg O2/kg/jam di 20oC. Nilai rata-rata konsumsi oksigen terendah terjadi pada jam 02.00 dan 17.00, yaitu 24,5 ± 1,9 mg O2/kg/jam. Sedangkan nilai konsumsi oksigen tertinggi tercatat pada jam 06.00, yaitu 29,1 ± 1,7 mg O2/kg/jam. Berdasarkan data konsumsi oksigen yang diperoleh dari penelitian ini, tidak ditemukan adanya perbedaan nyata antara konsumsi oksigen induk ikan mas saat siang dan malam hari dengan adanya manipulasi fotoperiode. Aktivitas dan metabolisme induk ikan mas mengalami perubahan dengan adanya perubahan fotoperiode.Kata kunci: Ikan mas, Cyprinus carpio, konsumsi oksigen, fotoperiode.

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