Biosaintifika: Journal of Biology & Biology Education
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
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<![CDATA[Detection of Powdery Mildew Resistance Gene in Melon Cultivar Meloni Based on SCAR Markers ]]>
<![CDATA[Muhammad Alif Ishak]]>;
<![CDATA[Budi Setiadi Daryono]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.22198
<![CDATA[Powdery mildew is one of the diseases caused by fungal infections that can reduce the production of melon fruit worldwide including in Indonesia. A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. This study aimed to detect resistance gene linked to powdery mildew using a sequence characterized amplified region (SCAR) markers. The melon cultivar Meloni was used in this study. SL-3, PI 371795, and Aramis cultivar were used to compare. Amplification of the marker was performed employing a pair of primers. The result showed that Meloni had a powdery mildew resistance gene by the presence of a DNA target band at 1058 base pair (bp). Based on this result, it could be concluded that Meloni was an excellent melon cultivar because of its ability to overcome the powdery mildew infections naturally. SCAR markers have been used for various purposes, especially to detect resistance genes to plant diseases. The present study had provided information for plant breeders about Meloni as the new melon cultivar that was genetically resistant against powdery mildew infections. Furthermore, Meloni could be proposed as an alternative to native Indonesian superior melon seeds.]]>
<![CDATA[Isolation and Identification of Actinomycetes Associated with Moss on the Surface of the Borobudur Temple Stone ]]>
<![CDATA[Ade Lia Putri]]>;
<![CDATA[Debora Christin Purbani]]>;
<![CDATA[Atit Kanti]]>;
<![CDATA[Mia Kusmiati]]>;
<![CDATA[Moh Habibi]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.20334
<![CDATA[Mosses growing on the surface of the Borobudur Temple will affect the aesthetic value of the temple. Interaction between moss and actinomycetes may trigger the growth of moss that can cause an increase in biodeterioration of stone. The purpose of this study was to isolate and identify the actinomycetes associated with moss on the surface of decayed stone of Borobudur Temple as well to assess their ability for phosphate solubilizing. Actinomycetes were isolated using serial dilution method and were identified based on 16S rRNA gene sequences. A total of 37 actinomycetes were isolated from three sampling sites. The isolates found belong to five genera (Gordonia, Microbacterium, Micromonospora, Nocardia, and Streptomyces) and distributed among four families (Microbacteriaceae, Micromonosporaceae, Nocardiaceae, and Streptomycetaceae). Isolates of actinomycetes composed of 19 Streptomyces Group and 18 Rare Actinomycetes Group. Nineteen isolates (51.35%) were identified as genus Streptomyces. Seventeen isolates (45.94%) showed abilities to release soluble phosphate and most of the isolates belong to the genus Streptomyces. The isolates have been collected will be deposited to Indonesian Culture Collection (InaCC) to enrich the collection of actinomycetes from ancient stone in Indonesia and will be used as a source of reference material research, taxonomic, or as source for further study.]]>
<![CDATA[Potentials of Carbon Stored in Plant Biomass at Little Farmers Grassland Cisarua, West Bandung Regency ]]>
<![CDATA[Annas Dwitri Malik]]>;
<![CDATA[Komang Yoga Zso Zsa Dewa]]>;
<![CDATA[Parikesit Parikesit]]>;
<![CDATA[Susanti Withaningsih]]>;
<![CDATA[Ratna Wingit]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.23111
<![CDATA[Alternatives of vegetations to store carbon need to be encouraged considering that forests are threatened by widespread destructions. One such vegetation is grasslands which have the potential for carbon storage and to reduce CO2 concentration in the atmosphere. At present, many enterprises have designed grasslands for animal feed. Grassland at Little Farmers, Cisarua, West Bandung was established for many purposes, i.e. recreation, education, and animal feed. The purpose of this research was to study the potential of carbon stock in grassland vegetation at this location. Based on RaCSA method, the tree biomass was determined by nondestructive collection of density and basal area of trees, then calculated by an allometric equation. The ground cover biomass was determined by destructive collection of grass and roots. Total measured biomass was multiplied by 46% to obtain carbon storage. Based on the results, the potential of carbon stock in Little Farmers grassland is 6,506.23 kg/m2 with the potential for carbon storage below the ground (0.129 kg / m2) was slightly higher than the carbon stored above the ground  (0.101 kg / m2). Carbon stored in ground cover had been proven to be lower than woody plants (6,506 kg / m2). This study has pioneered in finding the carbon stocks potential of a man-made grassland, so it provides basis of an alternative land use that can be encouraged for carbon sequestration. For many enterprises, this study will aid in the conduct and management planning of grasslands with regards to ecosystem services preservation, such as carbon sequestration.]]>
<![CDATA[Anthracnose Disease on Vegetables Crops in Serang Village, District of Karangreja, Purbalingga Regency ]]>
<![CDATA[Eddy Tri Sucianto]]>;
<![CDATA[Muachiroh Abbas]]>;
<![CDATA[Endang Sri Purwati]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.21818
<![CDATA[Various vegetables are widely cultivated in Serang Village, District of Karangreja, Purbalingga Regency. This village is located at 1350 above sea level. Vegetable productions in Serang are often constrained by the presence of diseases caused by pathogenic fungi Colletotrichum sp. called anthracnose. However, there was no data regarding anthracnose attack in vegetable crops in Serang. This study aimed to find out the types of vegetable that were attacked by anthracnose disease as well as the intensity of the attack in a vegetable crops in Serang Village, Karangreja District, Purbalingga Regency. Vegetables with anthracnose were purposively selected from 10 different locations. The anthracnose disease was identified based on their symptoms and analyzed descriptively. The results showed that five different vegetable crops suffer from anthracnose, and the intensity of anthracnose ranges from 32% up to 52%. Those intensity percentages indicate that large part of vegetable crops in Serang Village was attacked by anthracnose and possibly cause a significant decrease in vegetable production in that village. Our results provide the first scientific data about anthracnose attack in Serang Village. The data is essential for the management of vegetable crops in Serang Village.]]>
<![CDATA[The Potential of Cnidoscolus chayamansa Alchoholic Leaves Extract as Hypolipidemia Agent ]]>
<![CDATA[Retno Sri Iswari]]>;
<![CDATA[Ibnul Mubarok]]>;
<![CDATA[Fitri Arum Sasi]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.23687
<![CDATA[Cardiovascular disease caused by hyperlipidemia is the highest case in term of fatality of non-infectious disease in Indonesia. Cnidoscolus chayamansa is well known as a herb that has nutraceutical potential for medicine and likely as a hypolipidemia agent. Therefore, this study aimed to identify the optimal dose of C. chayamansa as a candidate to treat cardiovascular diseases. A total of 30 white rats were acclimatized in the laboratory for 14 days. After that, the rats were grouped into six groups, randomly, which including a healthy control group (K0), a negative control group or hyperlipidemic rats (K-), a positive control group or hyperlipidemia-induced rats that was supplemented with atorvastatin (K+), then a hyperlipidemic rats treated with alcoholic extract of C. chayamansa leaves at doses of 100 mg /kgBB /day for K1, 200 mg/ kgBB/ day for K2 and 400 mg/ KgBB/ day for K3. The results showed that K3 treatment is the most effective and optimum dose for lowering total cholesterol level at 76.81±1.10 mg/dl, triglycerides 72.39±1.66 mg/dl and LDL-C at 21.47±0.58 mg/dl. The aortic histology assessment also showed that the K- group had putative thrombus or plaque in intima, and it was not found in other groups. This research focused on the optimum dose and new usage of C. chayamansa, as an anti-inflammatory in atherosclerosis. By understanding the optimum dose of C. chayamansa, the community can apply and control the herb consumption for their own therapeutic properties. For future application, the herb is potentially developed as anti-atherogenic medicine.]]>
<![CDATA[Application of Rice Root Endophytic Bacteria in Ciherang Variety Rice (Oryza sativa) Seeds ]]>
<![CDATA[Lenni Fitri]]>;
<![CDATA[Yulia Sari Ismail]]>;
<![CDATA[Putriani Putriani]]>;
<![CDATA[Warzatullisna Warzatullisna]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.21697
<![CDATA[Endophytic bacteria are microorganisms that thrive in plant tissue without causing any negative effects to the host plants. The purpose of this study was to apply endophytic bacteria that have the potential to produce IAA hormones as biological agents in rice seeds. This research was conducted by testing IAA hormone using a spectrophotometer, testing HCN production using filter paper, biochemically identification of the isolate, and applicating endophytic bacteria into rice seeds. Endophytic bacteria have different abilities in producing IAA hormone. Based on the results of the study, the highest IAA concentration (57 ppm) was obtained from KSB1 (Potassium Solubilizing Bacteria) isolates and followed by KSB3 isolate with 56 ppm. All the endophytic bacteria isolates were unable to produce HCN. Results of the study showed that KSB1 and KSB3 isolate were able to influence the growth of root length by 5.62 cm and 6.70 respectively as well as the canopy height by 8.56 cm and 8.30 cm respectively. Based on observations of colony morphology, cell morphology and biochemical tests, KSB1 was presumably belong to the genus Staphylococcus. In this study, new information about endophytic bacteria that can increase the growth of Ciherang variety rice can be obtained. This study provides information that the application of environmentally friendly bacteria is important to preserve the environment and agriculture sustainability in Indonesia without reducing the productivity of rice.]]>
<![CDATA[Heritability Analysis of Local Corn Cultivars from Kisar Island Southwest Maluku After Induced with Colchicine ]]>
<![CDATA[Hermalina Sinay]]>;
<![CDATA[Jonetha Tanrobak]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.19847
<![CDATA[In the plant breeding program, selection is one process which aimed to obtain some varieties with superior traits. Within this process, one of criteria that must be consider is the wide genetic diversity of the plant which was selected, and how these traits are inherited. The purpose of this research was to determine (1) the influence of cultivar differences and the colchicine concentration towards the growth and production of local corn cultivar from Kisar Island Maluku, (2) to determine the heritability of growth and production variables after induced with colchicine. Completely randomized block design was used in this research with factorial design that consist of two factors i.e corn cultivar and colchicine concentration. The research procedure starts from field and seed preparation, immersion of seed into the colchicine solution, transplantation of seed into the field, plant maintenance, and  harvesting. The variable measured including growth and production variables of corn. The analysis of variance showed a significant effect of cultivar differences on all growth and production variables (p value 0.05), while colchicine concentration only showed a significant effect toward the plant height and number of seed per ear.  The heritability analysis result showed that all variables observed were in high category. The different corn cultivars have a significant effect on all the growth and production, while the colchicine concentration only affect the plant height and number of seed per ear, and the heritability were in high category.  This research provides useful information to farmers how to use colchicine to trigger plant growth and production. It can be used as an information in selecting corn cultivars with high yield potential.]]>
<![CDATA[Growth Performance, Yield Component and Genotypic Response of Mungbean (Vignaradiata l.) Against Soil Borne Diseases ]]>
<![CDATA[Emerensiana Uge]]>;
<![CDATA[Rudi Iswanto]]>;
<![CDATA[Ratri Tri Hapsari]]>;
<![CDATA[Trustinah Trustinah]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.23827
<![CDATA[Soil-borne disease caused by fungus infection is one of the disease that causes the death and crop failure of mungbean. The aim of this study was to identify the performance, yield component and respons of plant to the disease. Performance of 25 genotypes were observed in installations research and assessment of agriculture technology (IPPTP), Jambegede of Indonesian Legumes and Tuber Crops Research Institute (ILETRI)at dry season 2018 using RCBD with three replications. The result showed that each variety has different characteristic. The plant with the highest number of plant height was genotype 15 and 18, while the total branches of 25 genotypes were generally similar (4).The highest number of grown and harvested plant was genotype 15. The genotypes with shorter flowering, maturity and harvesting age were 23, 20, 18, 15 and 10. The highest average number of filled pods was genotype 11 and empty pods was genotype 13. There were 8,10 and 2 genotypes with seeds weight of 7, 6 and 6 respectively. The highest yields were obtained from genotype 19 and 3 by total of yield of 2.0 t/ha and 1,9 ton/ha respectively. Resistance test against soil-borne diseases resulted 5 genotypes that were moderate resistant observed at 2 DAP. This research contributed to gave information about new genotypes of mungbean that potentially produce new superior varieties.]]>
<![CDATA[Effect of Induced Polyploidy on Plant Growth, Chlorophyll and Flavonoid Content of Artemisia cina ]]>
<![CDATA[Sri Kasmiyati]]>;
<![CDATA[Elizabeth Betty Elok Kristiani]]>;
<![CDATA[Maria Marina Herawati]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.22548
<![CDATA[Artemisia cina is one of a member of genus Artemisia that has potential as a medicinal plant. However, the levels of Artemisia medicinal bioactive compounds are very low. Polyploidization is an alternative method that can enhance of growth and secondary metabolite productions of plants. The aims of this research were to determine the effect of polyploid induction using colchicine and plant growth regulator toward plant growth, the chlorophyll, kaemferol and quercetin contents of A. cina. Four different A. cina used in this research consisted of two diploid genotypes (TWN and KJT) and two polyploid genotypes (J and M). Induction of mutant polyploid was conducted using colchicine and combination of plant growth regulator benziladenyl (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The measured plant growth parameters were dry weight, leaf area, and plant height. The chlorophyll content of leaves was determined spectrophotometrically, and flavonoid content determined using HPLC. The result showed that the polyploid genotype (M) significantly decreased leaf and root growth compared to the KJT and TWN. In A. cina plants, polyploidization using colchicine is more effective in increasing the biomass than using combination plant growth regulator BA and 2,4-D. The flavonoid content of KJT was the lowest, and significantly different compared to the other plants. This study provides new information about the effect of polyploid on growth and flavonoid content in A. cina. This can be  useful information to develop A. cina to become a medicinal plant.]]>
<![CDATA[Phycocyanin from Spirulina platensis Induces Cytotoxicity and Apoptosis in T47D Cells ]]>
<![CDATA[Safira Chairani Dimarti]]>;
<![CDATA[Neni Susilaningsih]]>;
<![CDATA[Renni Yuniati]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v12i1.22992
<![CDATA[Breast cancer cases in Indonesia have risen in alarming rate. Herbal medicines have been developed to complement conventional cancer therapy and to lessen the negative effects of the therapies. Phycocyanin, blue-green photosynthetic pigment from Spirulina platensis, becomes a potential candidate of herbal medicine for cancer therapy due to its capability to eliminate various cancer cell line on in vitro level. This research aimed to analyse the effectivity of phycocyanin to induce cytotoxic and apoptotic activity of T47D cells (breast cancer cell line). MTT assay was perform to measure IC50 value. Double-staining assay and flow cytometry were applied to visualize the appearance of apoptotic cells and to measure the percentage of apoptotic and necrotic cells. Result showed that the IC50 value of phycocyanin towards T47D cells is 1650 μg/mL. Phycocyanin was also proven to induce apoptosis at IC50 with the apoptosis percentage of 45.93%. This research presented different results from previous researches that used phycocyanin from saltwater Spirulina platensis. Based on the results of this study, phycocyanin has the potential to be developed as anticancer agent by inducing apoptosis.]]>
