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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biosaintifika: Journal of Biology & Biology Education
Budi Setiadi Daryono
Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Detection of Powdery Mildew Resistance Gene in Melon Cultivar Meloni Based on SCAR Markers Muhammad Alif Ishak; Budi Setiadi Daryono
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22198

Abstract

Powdery mildew is one of the diseases caused by fungal infections that can reduce the production of melon fruit worldwide including in Indonesia. A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. This study aimed to detect resistance gene linked to powdery mildew using a sequence characterized amplified region (SCAR) markers. The melon cultivar Meloni was used in this study. SL-3, PI 371795, and Aramis cultivar were used to compare. Amplification of the marker was performed employing a pair of primers. The result showed that Meloni had a powdery mildew resistance gene by the presence of a DNA target band at 1058 base pair (bp). Based on this result, it could be concluded that Meloni was an excellent melon cultivar because of its ability to overcome the powdery mildew infections naturally. SCAR markers have been used for various purposes, especially to detect resistance genes to plant diseases. The present study had provided information for plant breeders about Meloni as the new melon cultivar that was genetically resistant against powdery mildew infections. Furthermore, Meloni could be proposed as an alternative to native Indonesian superior melon seeds.
INTRA-SPESIFIC DIVERSITY OF BUTTERFLY PEA (Clitoria ternatea L.) REVEALED BY ISSR WITH INVARIABLE ITS RECORDS Adib Fakhruddin Yusuf; Vida Rahma Latifah; Vivi Indah Nurcahyati; Anggun Diyan Nurhasanah; Adristi Shafa Widyasari; Ananto Puradi Nainggolan; Aldy Riau Wansyah Hasibuan; Madyan Akmal Hidayat; Karmilah Karmilah; Arini Dian Pratiwi; Rindu Aurantika; Muslifah Hasanah; Ganies Riza Aristya; Niken Satuti Nur Handayani; Tuty Arisuryanti; Indra Lesmana; Budi Setiadi Daryono
BIOTROPIA Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.1.2362

Abstract

Article Highlights:- The ITS region exhibits no genetic variation among butterfly pea genotypes.- ISSR reveals high polymorphism, proving effective for genetic diversity analysis.- White single and purple single genotypes show the greatest genetic distance.- ISSR-6 ((CT)₈TG) demonstrates the highest polymorphism and reliability for assessment.- The ITS sequences have been deposited in GenBank under the accession IDs PQ198055–PQ198061. ABSTRACTClitoria ternatea L., a perennial plant in the Fabaceae, is recognized for its resilience in tropical climates and its diverse applications in both culinary and medicinal fields. However, the limited exploration of its genetic diversity constrains breeding efforts aimed at improving desirable traits. This limitation highlights the need to optimize selection strategies, identify superior genotypes, and preserve valuable genetic resources for long-term conservation and crop enhancement. This study aimed to explore genetic variation using molecular markers to analyze C. ternatea genotypes based on petal architecture and color differences. To assess the genetic diversity of C. ternatea, Sanger sequencing of the Internal Transcribed Spacer (ITS) region and Inter-Simple Sequence Repeat (ISSR) markers were applied to seven wild populations from Sleman, Yogyakarta. The ITS region exhibited no genetic variation, indicating its conserved nature and limited ability to differentiate genotypes. In contrast, ISSR markers effectively detected genetic variation, identifying 62 polymorphic fragments out of 162 total bands. The highest genetic distance (0.297) was observed between the WS and PS genotypes, whereas the double-petal genotypes (WD and PD) displayed the closest phenetic relationship. Among the ISSR primers, UBC-808, UBC-812, and ISSR-6 exhibited high PIC and RP values, confirming their reliability in genetic diversity analysis. These results underscore the utility of ISSR markers as a robust tool for genetic diversity assessment, offering valuable insights for breeding programs and germplasm conservation in C. ternatea.
Co-Authors Adib Fakhruddin Yusuf Adristi Shafa Widyasari Aldy Riau Wansyah Hasibuan Ananto Puradi Nainggolan Anggun Diyan Nurhasanah Arini Dian Pratiwi Ganies Riza Aristya Indra Lesmana Karmilah Karmilah Madyan Akmal Hidayat Muhammad Alif Ishak Muslifah Hasanah Niken Satuti Nur Handayani Rindu Aurantika Tuty Arisuryanti Vida Rahma Latifah Vivi Indah Nurcahyati