cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Dr. Ir. Dyah Rini Indriyanti
Contact Email
biosaintifika@mail.unnes.ac.id
Phone
-
Journal Mail Official
biosaintifika@mail.unnes.ac.id
Editorial Address
-
Location
Kota semarang,
Jawa tengah
INDONESIA
Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 54 Documents
 2   3   4   5   6 
Search results for , issue "Vol 12, No 3 (2020): December 2020" : 54 Documents clear
Antioxidant Activity of Jojoba (Simmondsia chinensis) Seed Residue Extract Arnold Patogi Siahaan; Eti Rohaeti; Ali Mahmoud Muddathir; Irmanida Batubara
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23323

Abstract

Jojoba (Simmondsia chinensis (Link)) is a shrub plant that widely used as cosmetic ingredients especially jojoba oil. When making jojoba oil, the residue still reminds and become waste. This study aimed to determine the antioxidant activity of jojoba seed residue (JSR). Jojoba seed residue was collected from Sudan. The JSR extracted by maceration with n-hexane, ethyl acetate, and 70% ethanol solvent. The antioxidant activity was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. The result showed that 70% ethanol extract of JSR had the highest antioxidant activity with 50% Inhibition Concentration (IC50) value of 219.42 mg/L. The 70% ethanol extract was fractionated by using silica gel column chromatography with gradient elution produced 10 fractions. All fractions showed antioxidant activity (IC50106-447 mg/L). Fraction 3 had the highest antioxidant activity with IC50 value of 106.26 mg/L. Therefore, JSR has a great potential for use as a raw material in cosmetic and pharmaceutical products industry, or as a source of bioactive compounds.
Plantlet Formation and Acclimatization of Sugarcane cv. PS 881 with Different Types and Concentration of Auxin Rendie Prasetyo; Sugiyono Sugiyono; Elly Proklamasiningsih; Prita Sari Dewi
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23482

Abstract

This research has been carried out with a view to induce rooting and  plantlet formation, followed by acclimatization.  Micro shoots of sugarcane cv. PS 881 were cultured on Murashige and Skoog medium supplemented with different types and concentration of auxins for root induction. This research conducted experimentally using a split-plot design. The main plots were three types of auxins, which consisted of IAA, IBA, and NAA. The subplots were auxin concentrations with four levels, i.e. 0 μM, 5 μM, 10 μM, and 15 μM. Significantly faster root emergence time and higher number of roots observed in the Murashige and Skoog basal medium supplemented with 10 μM NAA. The best root length obtained in the Murashige and Skoog basal medium supplemented with NAA 0 μM. Plantlets derived from NAA 10 μM treatment showed the best performance during acclimation with a 100% survival rate. NAA at a concentration of 10 µM considered to be the best treatment in  plantlet formation and acclimatization of sugarcane cv. PS 881. This study showed that the use of MS medium with 10 uM NAA is able to increase the growth of PS 881 sugarcane plantlets. The results of this study can increase the availability of high quality seedlings and increase national sugar production.
Alkaline Phosphatase Expression From Mice Mesenchymal Stem Cells Induced By Flamboyant Flower (Delonix regia) Extract Kartini Eriani; Deby Anggraini; Yudha Bintoro; Ichsan Ichsan; Al Azhar; Silmi Mariya
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25433

Abstract

Flamboyant flower (Delonix regia) extract can increase proliferation and differentiation rates of mesenchymal stem cells (MSCs) into specific cells such as bone, nerve, and fibroblast cells. The extract possess metabolic compounds that may act as antibiotics, anti-inflammatory, antimicrobial, diuretic, anthelmentic, astringent, leucorrhoea, and potentially increase the body's metabolism normally. This study aimed to investigate expression level of alkaline phosphatase (ALP) by mice MSCs treated with flamboyant flower extract in vitro. Here, mice bone marrow cell cultures were treated with flamboyant flower extracts of 0.6 mg/ml (P1), 0.7 mg/ml (P2), 0.8 mg/ml (P3), and 0.9 mg/ml (P4). Untreated cell culture was used as negative control (P0). Expression of ALP gene was measured by RT-qPCR method. The results showed that mice mesenchymal stem cell could differentiate into bone, nerve, and fibroblast cells. The addition of flamboyant flower extract ranged from 0.6-0.9 mg/ml significantly (p0.05) influenced the expression of ALP by differentiating MSCs. The highest expression was found at the stem cells treated with flamboyant flower extract of 0.8 mg/ml, 0.13 times compared with control. In conclusion, flamboyant flower extracts treatment might increase the expression of ALP in differentiating MSCs.  This information can be used as a basis for finding an appropriate biomarkers for tracking the differentiation and profileration of tissue originated MSCs induced by extracts of medicinal plants.
Carotenoid Production by Rhodosporidium paludigenum Using Orange Peel Extract as Substrate Renna Eliana Warjoto; Jennifer Jennifer; Bibiana Widyawati Lay
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24867

Abstract

Carotenoids are fat-soluble pigments that have various benefits in health and beauty. The demand and market of carotenoids are increasing; thus, the faster and cheaper biological production of these pigments using microorganisms is desired. In this study, the optimum medium pH and nitrogen concentration for carotenoid production by Rhodosporidium (R.) paludigenum using orange peel extract as substrate were determined. The soluble sugars from orange peels were extracted using distilled water at 100 °C. The yeast inoculum was cultivated in Yeast Peptone Dextrose (YPD) media before fermentation. The independent variables in the fermentation factorial design included initial medium pH (5; 6; and 7) and nitrogen concentration (0; 1; and 1.75 g/L urea in the medium). The decrease in reducing sugar concentration and the increase in biomass dry weight during fermentation demonstrated carbon source consumption by R. paludigenum for growth and carotenoid production. On the sixth day of fermentation (D6), the culture was harvested for the total carotenoid extraction and determination. The highest total carotenoid yield (107.63 µg/g) was achieved by the treatment group with an initial medium pH of 6 without nitrogen supplementation. This result indicated the orange peel extract potential as a substrate for carotenoid production using R. paludigenum as a workhorse. This was the first reported research in natural carotenoid generation using R. paludigenum (strain InaCC Y-236) locally isolated in Indonesia as the producer and orange peel extract as the substrate. Thus, this research would enrich the microbial carotenoid exploration and waste valorization to value-added products at the same time. The study can then be developed further and upscaled for industrial applications. 
Anti-tumor Potential Ethnomedicinal Plants from Dayak Tribes in Central Kalimantan Liswara Neneng; Asri Pujirahadju; Ferayani Ferayani; Tri Widayat
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23671

Abstract

Hornstedtia conica Ridl., Microsorum pustulatum Copel and Rourea fulgens Planch were medicinal plant used by  people of Dayak Tribes in Central Kalimantan for anti-tumor medicines. Although these plants has been widely used in traditional medicine, however, research and scientific evidence about their potential as anti-tumor are still very limited.  The research aims were to determine the qualitative content of phytochemical compounds and to study anti-tumor potential of these medicinal plants. This study used female Swiss Webster albino as a test animal.  Induction of tumor cell in mice used 7,12-Dimethylbenz [a] anthracene (DMBA), was done 10 times for 5 weeks.  Experiments were carried out used hexane extract from medicinal plants, every day for 7 weeks. The data obtained were analyzed descriptively. The results as follows: extracts of Hornstedtia conica Ridl., and Microsorum pustulatum Copel. contained alkaloids, while extract of Rourea fulgens Planch. contained flavonoids and saponins. All of these plant extracts were able to reduce the size and number of mice tumors. Hexane extract of H. conica was able to reduce the tumor size faster than another plants extracts. Mortality rate of mice in hexane extracts of H. conica and R. fulgens were zero- and in hexane extract of M. pustulatum it was 30%, meanwhile almost all mice in control group were died in days 15 of treatment. Based on this study, it can be concluded that hexane extract of H. conica and R. fulgens are potential as anti-tumor medicinal plants. These findings scientifically proved the potential of medicinal plant extracts as anti-tumor compounds.  H. conica is the most potential one for further development as alternative drug for inhibited of tumor growth.
Total Phenol Content of Avicennia marina Leaf and Its Relationship to the Environmental Quality Endah Dwi Hastuti; Munifatul Izzati; Sri Darmanti
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23957

Abstract

Environmental dynamic in the coastal area is suggested to affect the metabolite concentration in mangrove plants. This research aimed to study the concentration of total phenol in A. marina leaf and to analyze the effect of environmental parameters on total phenol content dynamics. Environmental parameters studied in this research was temperature, pH, DO, and salinity, as well as N, P, and C sediment content. Data analysis was carried out through multiple regression of natural logarithm transformed data. Laboratory analysis resulted the value of total phenol content in A. marina leaf ranging from 0.88 – 1.62% with the average concentration of 1.28 ± 0.28%. Regression analysis resulted the significant effect of temperature, DO, and sediment content P and C expressed in the formula: ln(TP) = 31.229 – 7.224ln(T) – 0.067ln(DO) – 1.054ln(P) – 1.241ln(C). The research implicated that the increasing value of those factors was approaching the suitable condition for A. marina. Thus, instead of increasing the phenol concentration, the parameters negatively effect the secondary metabolite. The result showed that increasing temperature, DO, and content of P and C reduced the stress in A. marina and reduce total phenol content. This suggests that low temperature, DO, P and C concentration provides more potential of phenolic products from A. marina. 
The Antioxidant Content and Activity of Various Plant Organs of Kitolod (Isotoma longiflora) Grahita Rezhi Egarani; Sri Kasmiyati; Elizabeth Betty Elok Kristiani
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23888

Abstract

Antioxidants play a crucial role in human health owing to their ability to neutralize oxidative reactions. Kitolod (Isotomalongiflora) is one of the medicinal plants that can be used as a source of antioxidant. The aim of study wasto compare the content of antioxidant compounds (flavonoids, phenolics, chlorophylls, carotenoids, and ascorbic acid) in various organs of the kitolod plant. The plant organs studied includingroots, stems, leaves, flowers, and fruits. The extraction of each plant organ wasconducted by maceration technique using ethanol as a solvent at the room temperature for 48 hours. The analysis of total flavonoids, phenolics, and ascorbic acid was performed by colorimetry method using AlCl3, Folin, and sulfosalicyclic acid reagent respectively, while chlorophylls and carotenoids was determined by direct colorimetry method with methanol as a solvent. The antioxidant activity of various plant organs of kitolod was analyzed using the DPPH method. The highest total flavonoid, phenolic, chlorophyll, and carotenoid compounds werefound in leaves, respectively by 10.48, 1.46, 7.25, and 56.98 ppm. The highest ascorbic acid content and antioxidant activity were obtained fromfruits.The research findings provide new and important information aboutthe contents and antioxidant activity of the secondary metabolites (flavonoid, phenolic, chlorophyll, carotenoid, and ascorbic acid) in each organ of kitolod plant. The information from the results of this study can be used to increase the medicinal value ofkitolod plants.
Isolation of Potential Plant Growth Promoting Rhizobacteria (PGPR) from Cassava (Manihot esculenta) Rhizosphere Soil Suri Raihan Safriani; Lenni Fitri; Yulia Sari Ismail
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.25905

Abstract

Cassava is the third most important calorie source in tropical area after rice and corn. The microorganism associated with cassava roots may be potent and useful for application to promote plant growth. Plant growth promoting rhizobacteria  is a group of benefical bacteria that live in rhizosphere. The aim of this study was to isolate and to identify the potential Plant Growth Promoting Rhizobacteria (PGPR) from cassava rhizosphere soil. The study consisted of isolation and identification of bacteria based on morphological and biochemical characters, hypersensitive reaction test, the ability to solubilize potassium and phosphate, and the ability to inhibit the growth of pathogen Sclerotium rolfsii. A total of nine bacteria isolates were succesfully isolated from Cassava rhizosphere soil. Those isolates suspected as Micrococcus sp.1, Micrococcus sp.2, Micrococcus sp.3, Micrococcus sp.4, Micrococcus sp.5, Micrococcus sp.6, Neisseria sp.1, Neisseria sp.2 and Bacillus sp. All nine isolates did not show hypersensitivity reactions. Only Neisseria sp.1 and Neisseria sp. 2 were able to solubilize potassium and phosphate. All isolates were able to inhibit the growth of S. rolfsii. The highest inhibition was done by Micrococcus sp.4 (51.46 %). The physical and chemical properties of cassava rhizosphere soil affected the type of bacteria found in this study. The result confirmed that the potential isolates obtained from cassava rhizosphere soil can be plant growth promoters. The present study suggested that PGPR isolates might have potential in future field applications as plant growth promoters or biocontrol agents.
Science Web-Module Integrated with Tannery Waste as Local Potential to Improve Students’ Problem Solving Atznain Iqma Miswami; Heru Nurcahyo
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24356

Abstract

Problem solving skills are very important skill students must owned. The low score of students’ problem solving skills becomes a serious problem in the 21st century teaching and learning. The development of science web-based module integrated with tannery waste as local potential became a research that must be done. This research examined the appropriateness and the effectiveness of a science web-based module integrated with tannery waste as local potential in improving students’ problem solving skills. The Four-D research and development model was employed, consisting of define, design, develop, and disseminate. This research was conducted in the form of pretest posttest control group design. The appropriateness analysis conducted by content expert, teaching media expert, and science teacher indicating that the product was categorized very good. The effectiveness of the learning was tested using the independent sample t test with sig. value set at 0.000. The test showed that the science teaching done using the science web-based module was effective in improving students’ problem solving skills in SMP Negeri 1 Wungu. The product resulted from this study is expected to overcome the teaching and learning problems associated with the low score of students' problem-solving skills. Moreover, it can provide a real experience and independence to students in using the science web-based module to study.
Nutritional Content of Bone Flour Made from Plecos Fish Pterygoplichthys pardalis from the Ciliwung River, Indonesia Handhini Dwi Putri; Dewi Elfidasari; Haninah Haninah; Irawan Sugoro
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23881

Abstract

Pterygoplichthys pardalis bones that are thrown into the river is one source of pollutants in the Ciliwung River. P. pardalis bones have a high calcium content, they can be used as an alternative raw material for flour. The purpose of this study was to analyze the nutritional content of P. pardalis bone flour from the Ciliwung River. The research method used was a proximate analysis consisting of moisture, ash, fat, protein, and carbohydrates content analyses. Proximate analysis results showed that the P. pardalis bone plus head flour contained 3% moisture, 35% ash, 34% protein, 23% fat, and 5% carbohydrate. P. pardalis body bone flour contained 4% water, 37% ash, 37% protein, 19% fat, and 3% carbohydrate. Based on the proximate analysis results, P. pardalis bone flours did not met the national standard of SNI 2013 fish flour for quality I, II, and III. Until now, there has been no research that explain the nutritional content of bone flour of P. pardalis from Ciliwung River. This research provides information on the nutritional content of P. pardalis flour. Data obtained from the results of this study can be the basis for consideration in utilizing and processing P.pardalis from the Jakarta Ciliwung River.

Page 5 of 6 | Total Record : 54

 2   3   4   5   6 

Filter by Year

2020 2020


Reset
Filter By Issues
All Issue Vol 15, No 3 (2023): December 2023 Vol 15, No 2 (2023): August 2023 Vol 15, No 1 (2023): April 2023 Vol 14, No 3 (2022): Desember 2022 Vol 14, No 2 (2022): August 2022 Vol 14, No 1 (2022): April 2022 Vol 13, No 3 (2021): December 2021 Vol 13, No 2 (2021): August 2021 Vol 13, No 1 (2021): April 2021 Vol 12, No 3 (2020): December 2020 Vol 12, No 2 (2020): August 2020 Vol 12, No 1 (2020): April 2020 Vol 12, No 1 (2020): April 2020 Article-in-Press Vol 11, No 3 (2019): December 2019 Vol 11, No 2 (2019): August 2019 Vol 11, No 1 (2019): April 2019 Vol 11, No 1 (2019): April 2019 Vol 11, No 3 (2019): Article-in-Press Vol 10, No 3 (2018): December 2018 Vol 10, No 3 (2018): December 2018 Vol 10, No 2 (2018): August 2018 Vol 10, No 2 (2018): August 2018 Vol 10, No 1 (2018): April 2018 Vol 10, No 1 (2018): April 2018 Vol 9, No 3 (2017): December 2017 Vol 9, No 3 (2017): December 2017 Vol 9, No 2 (2017): August 2017 Vol 9, No 2 (2017): August 2017 Vol 9, No 1 (2017): April 2017 Vol 9, No 1 (2017): April 2017 Vol 8, No 3 (2016): December 2016 Vol 8, No 3 (2016): December 2016 Vol 8, No 2 (2016): September 2016 Vol 8, No 2 (2016): September 2016 Vol 8, No 1 (2016): March 2016 Vol 8, No 1 (2016): March 2016 Vol 7, No 2 (2015): September 2015 Vol 7, No 2 (2015): September 2015 Vol 7, No 1 (2015): March 2015 Vol 7, No 1 (2015): March 2015 Vol 6, No 2 (2014): September 2014 Vol 6, No 2 (2014): September 2014 Vol 6, No 1 (2014): March 2014 Vol 6, No 1 (2014): March 2014 Vol 5, No 2 (2013): September 2013 Vol 5, No 2 (2013): September 2013 Vol 5, No 1 (2013): March 2013 Vol 5, No 1 (2013): March 2013 Vol 4, No 2 (2012): September 2012 Vol 4, No 2 (2012): September 2012 Vol 4, No 1 (2012): March 2012 Vol 4, No 1 (2012): March 2012 Vol 2, No 2 (2010): September 2010 Vol 2, No 2 (2010): September 2010 Vol 2, No 1 (2010): March 2010 Vol 2, No 1 (2010): March 2010 Vol 1, No 1 (2009): March 2009 Vol 1, No 1 (2009): March 2009 More Issue