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INDONESIA
Biosaintifika: Journal of Biology & Biology Education
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 52 Documents
Search results for , issue "Vol 9, No 2 (2017): August 2017" : 52 Documents clear
The Potential of Flora and Fauna as Tourist Attractions in Biodiversity Park of Pelawan Forest, Central Bangka Henri Henri; Luchman Hakim; Jati Batoro
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9225

Abstract

Indonesia has a high potential for the diversity of flora and fauna species together with their ecosystem. Preservation of natural resources can be done through conservation using the concept of ecotourism. The purpose of this research is to identify the potential of the flora and fauna in Biodiversity Park of Pelawan Forest for tourist attraction. The study was conducted from October 2016 to January 2017 through inventory and in-depth interview. The analysis on the results of this study indicate that there are 41 species of 27 families of plants and 135 species of animals consisting of amphibians (6 species), reptiles (16 species), birds (99 species) and mammals (14 species). This indicates that Biodiversity Park of Pelawan Forest is very competitive for tourism attractions, which is supported by the presence of key species (Tristaniopsis merguensis), flagship species (Cephalopachus bancanus), and abundance of birds for bird watching. Based on the IUCN red list, several species of flora, such as Gonystylus bancanus, and fauna, such as Setornis criniger, Chloropsis sonnerati, Macaca nemestrina, Nycticebus menagensis, and Cephalopachus bancanus, are vulnerable to extinction. This study on flora and fauna results in the initial data that can be used to support conservation efforts. Moreover, the result of this study can provide an opportunity for visitors to enjoy these tourist attractions, which can benefit the local community.  
Retracted: Characterization of the Interaction between RIN13 (RPM1-Interacting13) and Sumo Proteins in Arabidopsis thaliana. This article has been retracted at the request of the author. The reason is due to poor quality of the publication, which may reflect poorly on the scientific quality of the authors. Venny Santosa; Mio Nagabuchi; Sachiko Okada; Katsunori Tanaka
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9487

Abstract

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The Effect of Water Content of Medium Containing Oryctes rhinoceros Larvae on Metarhizium anisopliae Pathogenicity Dyah Rini Indriyanti; Indah Nuraini; Muji Slamet
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.10262

Abstract

The entomopathogenic fungus, Metarhizium anisopliae (Metschnikoff) Sorokin (Ascomycota: Hypocreales)would effectively infect the target host on the appropriate medium water content. The aim of this study was to analyze the influence of water content of medium on the effectiveness of M. anisopliae fungus infection on O. rhinoceros larvae in the laboratory. Fifty healthy third instar larvae of O. rhinoceros were  obtained from field. The M. anisopliae obtained from Estate Crop Protection Board in Salatiga. The conidia density and viability of M. anisopliae were examined before used. The medium for maintaining the larva was the sawdust that had been sterilized. A total of 50 plastic cups were prepared to place 50 larvae (1 larva/cup). Each cup was filled with 100 g medium  of sawdust plus 2 g of M. anisopliae which was then stirred until mixed, with different water content: P1 (20%), P2 (40%), P3 (60%), P4 (80% ) and P5 (98%). The result indicated that  the water content of the medium affected the effectiveness of M. anisopliae fungus infection on O. rhinoceros larvae. The water content influenced the duration of larval mortality at each treatment. An important finding in this study is that controlling O. rhineceros larvae  with M. anisopliae can be done by manipulating the water content of medium. The benefit of this study may be used for the recommendation of O. rhinoceros pest control using M. anisopliae  with an effective water media content.
Effect of Seed Maturity and Storage Duration on Germination of Sambiloto (Andrographis paniculata) Solikin Solikin; Siti Nurfadilah
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8782

Abstract

Seed maturity and its storage are one of problems on propagation and developing of medicinal plants such as sambiloto (Andrographis paniculata (Burm.f.) ex Nees). The research aimed to determine effects of seed maturity and storage duration on seed germination of sambiloto was conducted in a green house of Purwodadi Botanical Garden from November 2014 to November 2015. The experiment was done by completely randomised design with two treatments namely seed maturity and storage duration. The seed was classified into immature seed (0.061 g/100 seeds), semimature seed (0.113 g/100 seeds) and mature seed (0.166 g/100 seeds). The seed’s storage duration was classified into seed was not stored, seed was stored for six months and seed was stored for twelve months. Each treatment combination was replicated five times. The results showed that there were significant interactions between the treatments on percentage and rate of seed germination. The highest percentage of seed germination was the treatment of mature seed and stored twelve months (98.50%). On the contrast, the lowest seed germination percentage was the treatment of immature seed and stored twelve months (4.25 %). The fastest seed germination rate was the treatment of mature seed and stored six months (3.88 days), whereas the slowest seed germination rate was the treatment of immature seed and without stored (28.58 days). This study is expected to be applied to improve genetic and cultivation of medicinal plant as well as increasing plant growth and yield.
Application of rps16 Intron and trnL-trnF Intergenic Spacer Sequences to Identify Rengas Clone Riau Dewi Indriyani Roslim; Herman Herman
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9108

Abstract

Rengas clone Riau has been identified using morphological characters and molecular technique with a psbA-trnH intergenic spacer, however, this method can only determine its taxonomic status at genus level, namely Gluta sp.  This study reports application two DNA barcodes, i.e. rps16 intron and trnL-trnF intergenic spacer, to identify Rengas clone Riau.  The methods included collection of the leaves from Kajuik Lake, total DNA isolation, electrophoresis, PCR (polymerase chain reaction), gel purification and sequencing.  The rps16 intron size was 659 bp and the trnL-trnF intergenic spacer was 527 bp. The BLASTn analysis showed that sequences of the rps16 intron and the trnL-trnF intergenic spacer of Gluta sp clone Riau had 100% similarity to those of G. renghas deposited in GenBank.  These results were supported by high max score, high total score, query cover = 100%, and E-value = 0.  The dendrograms also showed the closest relationship of Gluta sp clone Riau with G. renghas deposited in GenBank compared to other species of Gluta.  In conclusion, this study succeeded in identifying Rengas clone Riau as Gluta renghas by using sequences of the rps16 intron and the trnL-trnF intergenic spacer. A combination of DNA barcodes could be applied to identify various plants as long as the database for the DNA barcodes is available in public database such as GenBank. 
Resistance of Advanced Soybean Lines to Pod Borrer (Etiella zinckenella) Heru Kuswantoro; Marida S. Y. I. Bayu; Yuliantoro Baliadi; Wedanimbi Tengkano
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.7895

Abstract

The increasing and stabilizing of soybean product in Indonesia face many limitations. One of the limiting factors is pod borrer (Etiella zinckenella Treitschke) infestation that is able to cause yield loss up to 80%. Objective of the research was to find out some advanced soybean lines that resistant to pod borrer. Design was randomized complete block with three replications. Soybean lines were grown gradualy to ensure the simultanously flowering. The plants were caged at 35 days after planting (DAT) and infested with the imago of E. zinckenella at 56 DAT. Results showed that different soybean lines affected imago population, eggs population, larvae population, infected pods and infected seeds. Some genotypes were consistantly resistant to E. zinckenella. The resistance of those genotypes were non preference resistance based on eggs population, larvae population, infected pod and infected seeds. This study discovered nine soybean lines that is resistant to E. zinckenella, so that it can be beneficial for improving soybean resistance to this pest through releasing as a new resistant pod borer variety after tested further in potential yield and genetic x environment interaction trials. In addition, there were three varieties and two germplasm accessions that can be used as gene sources for improving the resistance of the varieties. The three varieties are able to be cultivated directly in field to decrease the E. zinckenella occurrence. 
Traditional Usages of Taro (Colocasia spp.) by Ethnic Communities in Borneo Linda Oktavianingsih; E. Suharyanto; Budi Setiadi Daryono; Purnomo Purnomo
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9956

Abstract

Borneo has a a wealth of various flora, including the Aroids (Araceae). Taro is one of the Aroids that cultivated and used as food crop since a long time ago by the people in Borneo. This study aimed to determine the utilization of Taros traditionally by several ethnic communities in Borneo. The research used Survey Explorative Method with Direct Interview Technique in the field. Taro samples were taken from various habitats of Banjar, Dayak, Kutai, Malay, Bugis, Toraja and China ethnic. The results showed that Taro species widely used are : Colocasia esculenta var. esculenta and var. antiquorum, Colocasia  affinis and wild of Colocasiaesculenta. C. esculenta var. esculenta and var. antiquorum is used as subsistence food crop and vegetables. Besides, it is used as medicine such as high blood pressure lowering and for consumption of diabetics. Parts of the plants consumed include leaves, petiole, corm and stolon. C. affinis is used as ornamental plants of the home garden, while wild C. esculenta  is used as animal fodder. Taro in Borneo have a considerable variation of traditional cultivars and vernacular names. Fifty eight traditional cultivars from 5 different habitat to be used by some ethnics.
Characterization of Ethanolic Extract of Streptomyces sp. as a Pancreatic Lipase Inhibitors Produced by Endophytic Streptomyces sp. AEBg12 Lenni Fitri; Anja Meryandini; Dyah Iswantini; Yulin Lestari
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8907

Abstract

Endophytic Streptomyces sp. AEBg12 isolated from Zingiber cassumunar (Bangle) is known to produce pancreatic lipase inhibitory compound. However, the characteristics of this active compound has not been reported yet. This study aimed to determine the characteristics of pancreatics inhibitory compound produced by Streptomyces sp. AEBg12 and to assess the role of endophytic actinobacteria in producing pancreatic lipase inhibitor using endophytic-free bangle tissue culture, wild bangle and compared with the activity of Streptomyces sp. AEBg12 endophytes. Supernatant of Streptomyces sp. AEBg12 was extracted using ethanol, ethyl acetate, and n-hexane solvents. Toxicity test was performed using larvae of shrimp Artemia salina. The results showed that the best solvent to obtain pancreatic lipase inhibitor compounds was ethanol. Phytochemical analysis showed that ethanolic extract of endophytic Streptomyces sp. AEBg12 contained flavonoids. IC50 value of ethanol extract was 180.83 µg/ml. The result of TLC showed that ethanolic extract of Streptomyces AEBg12 had a blue luminescence band indicated that there were either flavone, flavanones, flavonols or isoflavones. Inhibitory activity of Streptomyces sp. AEBg12 was higher than wild bangle and bangle tissue culture. The information from this study can be be used as a basic data for further characterization of the active compound, which might be developed as an antiobesity agent through its pancreatic lipase inhibitory activity.
Correlation of Physical-Chemical Parameters to Total Coliform Value in Jawi River, Pontianak, West Kalimantan Rahmawati Rahmawati; Laili Fitria; Liza Syafitri
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.10187

Abstract

Coliform bacteria can be used as an indicator of the presence of pathogenic bacteria, such as E.coli bacteria that cause diarrhea. The aimed of this study is to determine the relationship between physical-chemical parameters namely temperature, pH, DO and BOD to the density of coliform bacteria in Jawi River, Pontianak. The sampling was conducted at one point each in the upstream, midstream and downstream area of the Jawi River during two tidal conditions of the Kapuas River in September 2016 at 09:40 (at low tide) and at 15:40 (at high tide). The correlation of physical-chemical parameter to coliform value was tested Pearson Product Moment. The results showed that coliform bacterial density increased from upstream to downstream with 150-1500 MPN/100 ml at high tide and 930-11000 MPN/100 ml at low tide. The results showed that the coliform bacterial density value had a positive relation with pH and BOD parameters and negative relation with temperature and DO parameters. So, it can be concluded that there is a correlation between physical parameters, such as temperature and chemical parameters such as pH, DO and BOD to microbiological parameters especially Coliform bacterial density. The benefit of this study is to give information about water quality of Jawi River and its correlation with density of Coliform bacterial, so that people are expected to pay more attention to the use of clean water to avoid the disease caused by coliform.
Alteration of Gills and Liver Histological Structure of Cyprinus carpio Exposed to Leachate Tri Dewi Kusumaningrum Pribadi; Dzikrina Syahidah; Sairandri Dyah Harjanti; Desak Made Malini
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8972

Abstract

One of the main problems in the waste management in Indonesia is the treatment of leachate, which mostly dumped to the river This research is aimed to obtain information of histological alteration in gills and liver of C. carpio L. exposed to leachate. Measurements on the water quality parameters comprised water temperature, pH, and dissolved oxygen (DO). This research was conducted by exposing leachate to C. carpio for 96 hours. The concentration of leachate were 0 ppm, 80 ppm, and 100 ppm. Histological preparation were made on the gills and liver using 10% fixative Neutral Buffered Formalin and Ehrlich Hematoxylin-Eosin staining with qualitative observation descriptive analyses for discussion. The result showed that increasing water temperature is directly proportional to the leachate concentration in the aquaria, while the value of pH and DO inversely proportional to the leachate concentration. Damages on the gills with 80 ppm leachate concentrasion were identified as follows: fusion of secondary gill filaments and hyperplasia of epithelial cell, along with karyorrhexis and hydropic degeneration on the liver. Damages on the gills of fishes exposed to leachate with 100 ppm concentrasion were identified as follows: fusion of secondary gill filaments, hyperplasia of epithelial cell, congestion, and edema along with karyorrhexis, hydropic degeneration and melanomacrophage centre (MMC) found on the liver. The results of this study can be used as an overview of the impact of an environmental pollution by leachate as indicated from histological damage to the gills and liver of C. carpio, thus contribute significan information to aquaculture sector and endorse better waste management

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