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INDONESIA
Bioma : Berkala Ilmiah Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 25982370     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
BIOMA (ISSN 1410-8801) adalah Majalah Ilmiah Biologi yang bertujuan mewadahi semua informasi hasil penelitian, telaah pustaka, makalah teknis, dan kajian buku, dari berbagai cabang ilmu Biologi. Diharapkan penerbitan ini dapat berkontribusi dalam penyampaian informasi ilmiah yang merupakan mata rantai proses kegiatan ilmiah. Terbit sejak tahun 1999.
Arjuna Subject : -
Articles 12 Documents
 1   2 
Search results for , issue "Vol. 18, No.1, Tahun 2016" : 12 Documents clear
Karakterisasi Dan Identifikasi Molekuler Fusan Hasil Fusi Protoplas Interspesies Chlorella pyrenoidosa dan Chlorella vulgaris Menggunakan 18SrDNA Rismiarti, Asih; Kusumaningrum, Hermin Pancasakti; Zainuri, Muhammad
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.911 KB) | DOI: 10.14710/bioma.18.2.30-40

Abstract

Chlorella pyrenoidosa is a unicellular green algae that grows in fresh water with carotenoids consisting of             β-carotene, α-carotene, anthaxanthin, neoxanthin, zeaxanthin and lutein. C. vulgaris usually it lives in sea water with carotenoids, chlorophyll, tocopherol, ubiquinone and proteins. The quality of them is improved by protoplast fusion and identified further using moleculer analysis. This study aims to find out the characterization and identification of molecular fusan that is obtained from interspecies C. pyrenoidosa and C. vulgaris protoplast fusion process using 18SrDNA. Both C. pyrenoidosa and C. vulgaris are combined by protoplast fusion and then they were performed the isolation of DNA with CTAB modification method, followed by PCR gradient using primers 18S Chlorella and performed DNA sequencing. The result show that there are different characters between masterplan and fusan based on growth of fresh water and sea water medium. The success frequency of fusan as a result from protoplast fusion in the fresh water media is 21% and 6% for sea water medium. The results of the alignment between fresh water fusan and C. vulgaris masterplan from GeneBank shows that the base sequence homology is 93% C. pyrenoidosa masterplan from GeneBank is 90%. The result of molecular identification towards the sequence of fresh water fusan bases shows that there is a kinship relationship with the masterplan of C. pyrenoidosa 18S Chlorella and Chlorosphaera klebsii microalgae compared with some other species from Chlorophyta group with similarity value as many as 91%. It shows that the high variety genetic is based on variations of the base sequence and has a kinship with other species in the Chlorophyta group.                                      Keywords : Chlorella pyrenoidosa, Chlorella vulgaris, Protoplast Fusion, DNA Sequensing, 18SrDNA.
Struktur Komunitas Plankton Perairan Payau di Kecamatan Wedung Kabupaten Demak Zakiyyah, Isyarotuz; Hidayat, Jafron Wasiq; Muhammad, Fuad
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.686 KB) | DOI: 10.14710/bioma.18.2.89-96

Abstract

Aquaculture are highly prioritized by the government to support the economy. Products from the aquaculture sector is also targeted as a source of income to the Indonesian country. Aquaculture needs to improve to manage water quality, especially plankton to support natural food of the fish. This study aims to examines structure of plankton community in the brackish waters of the District Wedung and then examines the water quality with environmental quality standards of water the cultivation. Research was conducted in February-April 2015. The method used to analyze data that plankton with uses keanekargaman index (H ') and the perataan index ( e ). The results showed that the number of species (genera) plankton obtained during research in the waters of the District Wedung was 45 species, these types consist of 37 species of phytoplankton and zooplankton 8 types. The most common plankton species found in all the stations were Fragillaria sp, Synedra ulna, Oscillatoria formosa and Copepod nauplius. Index of species diversity that existed at the District Wedung moderate to high waters with a range from 1.69 to 2.91 therefore a complex community and stability of the ecosystem were moderate to high. The evennes index ranged from 0.63 to 0.85. Water quality based on the pH, salinity, Turbidity, N, P and organic materials are less good for the cultivation so that the necessary processing on the pond so that according to the criteria of the quality standards of water quality. Keywords: structure of plankton communities, brackish waters, District Wedung

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