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INDONESIA
Bioma : Berkala Ilmiah Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 25982370     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
BIOMA (ISSN 1410-8801) adalah Majalah Ilmiah Biologi yang bertujuan mewadahi semua informasi hasil penelitian, telaah pustaka, makalah teknis, dan kajian buku, dari berbagai cabang ilmu Biologi. Diharapkan penerbitan ini dapat berkontribusi dalam penyampaian informasi ilmiah yang merupakan mata rantai proses kegiatan ilmiah. Terbit sejak tahun 1999.
Arjuna Subject : -
Articles 12 Documents
 1   2 
Search results for , issue "Vol. 18, No.2, Tahun 2016" : 12 Documents clear
Kloning dan Sekuensing Gen Xilanase dengan Produk Gen Berukuran 30 kDa dari Bacillus halodurans CM1 pada Escherichia coli DH5α Safirah, Dearesty; Helianti, Is; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.71 KB) | DOI: 10.14710/bioma.18.2.167-172

Abstract

The paper industry contributed the environment pollution due to chlor substances. Utilization of alkalothermophilic xylanase enzyme as a biocatalyst in the production of paper may become an environmentally friendly biobleaching alternative. Bacillus halodurans CM1 produces xilanase enzyme that had optimal activity at pH 9 and temperature 70°C. Previous study showed that this CM1 strains has several xilanase genes. The cloning of one of these alkalothermophiic xylanase (alkxyn) gene has been already conducted. This study aimed to clone alkxyn gene that encode alkalothermophilic xylanase enzyme from B. halodurans CM1 into Escherichia coli DH5α. Amplification of alkxyn has been carried out using primers for amplification xylanase 30 kDa. The alkxyn gene fragment was inserted into pGEM-T Easy vector and then transformed into E. coli DH5α. The results showed that the recombinant of E. coli DH5α harboring alkxyn gene from B. halodurans CM1 has been obtained. The sequences analysist based on BLAST showed that alkxyn fragment has homology (99%) with the alkaliphilic xylanase gene from Bacillus sp. 31 which encodes alkaliphilic xilanase (Genebank assession number: JF912895.1). Keywords: cloning, Bacillus halodurans CM1, xylanase, alkalothermophilic.
Penggunaan Substrat Glukosa Berbagai Konsentrasi sebagaiSumber Karbon Microbial Fuel Cell Saccharomyces cerevisiae untuk Menghasilkan Energi Listrik Nurhakim, Muhammad Amal; Kusdiyantini, Endang; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (81.988 KB) | DOI: 10.14710/bioma.18.2.131-136

Abstract

The increases of human growth causes electrical energy demand’s expantion while the supply decreases drastically. Energy crisis had triggeredalternative renewable energy sourcesdevelopmentto substitutethe use ofoil that had beenmain energy resources for the people. Microorganisms utilization is used to produce electrical by researchers these years as an effort to actualize the goals. The system used is microbial fuel cell (MFC) technology which utilize metabolism activity from microorganisms to produce electrical energy. Microorganismswill perform metabolism bybreaking down glucose into hydrogen (H2) and oxygen (O2).Hydrogen has a role as raw material that used in reduction reaction with oxygen until it releases electron in anoda as electrical flows source. Saccharomyces cerevisiae is an example microorganisms that can utilize for produce electrical energy. This research aims to  find optimal concentration for glucose as a carbon source in microbial fuel cell Saccharomyces cerevisiaeto form electrical energy. This research use S. cerevisiae as microorganisms and variation of glucose concentration as a carbon source. Parameters measured in this study is the voltage (mV) and current (mA). Research’s result shows that glucose in 10 % (w/v) concentrate forms higher results in voltage (mV) and current (mA) compare to glucose with 20% (w/v) concentrate and in the concentrate of 30% (w/v) which values each 561,833 mV and 105,133 mA. Analysis of variance with level of confidence 95% shows glucose concentrates don’t react significantly voltage but react significantly on current. Tukey HSD’s test show significant different between current that was formed by glucose in the concentrate of 10% (w/v) compared to glucose in the concentrate of 20% (w/v) and 30% (w/v).Keywords : Saccharomyces cerevisiae, microbial fuel cell (MFC), glucose, electrical energy

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