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INDONESIA
SAINS DAN MATEMATIKA
Published by Universitas Diponegoro
ISSN : -     EISSN : -     DOI : -
Core Subject : Education,
Mathematics
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Volume 22 Issue 1 Year 2014" : 5 Documents clear
Respon Pertumbuhan dan Produksi Senyawa Antioksidan pada Kalus Hibiscus sabdariffa L. dari Eksplan yang Berbeda secara in vitro Noviati, Agustin; Nurchayati, Yulita; Setiari, Nintya
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 1 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Ascorbic acid and carotenoid are secondary metabolites found in roselle, which show antioxidant activity. These compounds can be obtained from callus induced  by several kinds of explants. The aims of this experiment is to study callus growth from explants which can encourage high level of antioxidant compounds. The callus was obtained from difference organ, i.e. section of leaf, petiole and flower sepal. Sterilized explants were planted in MS (Murashige&Skoog) combined with 2 mg/L Naphtalene Acetic Acid (NAA) dan 5 mg/L Benzyl Adenin (BA).  This experiment has been conducted by completly randomized design with 5 replicates. Besides fresh weight callus, callus respons from each explants were analyzed descrptively.  Ascorbic acid and carotenoid content were analyzed qualitatively and quantitatively by titration and spectrophotometric respectively.  The results showed that all kinds of explant dedifferentiated into callus which antioxidant content. Callus from leaf section had the higgest fresh weight with high level ascorbic acid. Whereas the higgest carotenoid level was obtained from callus-derived flower sepals. It conclused that  in vitro callus was useful for producing plant biochemical compounds. Keywords : Callus induction, antioxidant agents, explants, ascorbic acid, cartenoid
Penentuan Entrance Skin Exposure (ESE) pada Pesawat Mammografi Mammomat 1000 dengan Filter Molybdenum (Mo) dan Rhodium (Rh) Susanti, Fitri; Anam, Choirul; Setiawati, Evi
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 1 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Mammography is a radiographic examination to detect breast cancer earlier. However, the usage of radiation with low kVp can cause high dose and this becomes the reason why monitoring the dosage during the mammography process is an important thing to do. One of the techniques to monitor the radiation level is done based on the Entrance Skin Exposure (ESE) value. This research was aimed to determine the ESE value using molybdenum (Mo) and rhodium (Rh) as the filter at Mammomat 1000 mammographics system.The research was started by measuring the consistency of the X-ray tube voltage (kVp ). After that, the ESE value at the target/filter Mo/Mo and Mo/Rh was measured using a Barracuda Multi Purpose Detector (MPD). The tube voltage during the research was 26, 28, and 30 kVp with fixed electrical current-time 100 mAs. The result shows that ESE value resulted at the target/filter Mo/Mo and Mo/Rh is tolerable, except at tube voltage 30 kVp with target/filter Mo/Mo. The comparison of ESE value shows that Mo/Rh has lower ESE value than Mo/Mo.   Keywords: Entrance Skin Exposure (ESE), mammography, Barracuda Multi Purpose Detector (MPD)    Pemeriksaan mammografi merupakan tindakan efektif dalam mendeteksi adanya kanker payudara sejak dini. Namun demikian, penggunaan teknik radiasi dengan kVp rendah pada mammografi dapat menyebabkan dosis tinggi. Oleh karena itu, pemantauan dosis pada pemeriksaan mammografi sangat penting dilakukan. Salah satu teknik pemantauan dosis radiasi dapat dilakukan dengan nilai Entrance Skin Exposure (ESE). Penelitian ini bertujuan untuk menentukan nilai ESE dengan filter molybdenum (Mo) dan rhodium (Rh) pada pesawat mammografi Mammomat 1000. Penelitian diawali dengan pengukuran konsistensi keluaran tegangan tabung sinar-X (kVp output). Selanjutnya dilakukan pengukuran nilai ESE pada target/filter Mo/Mo dan Mo/Rh dengan menggunakan Multi Purpose Detector (MPD) merek Barracuda. Tegangan tabung yang digunakan dalam penelitian yaitu 26, 28 dan 30 kVp dengan arus-waktu tabung tetap yaitu 100 mAs. Hasil penelitian menunjukan bahwa nilai ESE yang dihasilkan pada target/filter Mo/Mo dan Mo/Rh masih berada dalam batas toleransi yang direkomendasikan, kecuali pada pada tegangan tabung 30 kVp untuk Mo/Mo. Hasil perbandingan nilai ESE menunjukan bahwa Mo/Rh memiliki nilai ESE yang lebih rendah dibandingkan dengan Mo/Mo.   Kata kunci : Entrance Skin Exposure (ESE), mammografi, Multi Purpose Detector (MPD) Barracuda.
Perhitungan Medan Listrik dan Rapat Arus pada Plasma Lucutan Korona dengan Konfigurasi Jarum–Cincin Sutanti, Ratna Wulan; Wardaya, Asep Yoyo; Nur, Muhammad
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 1 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

This paper presents analytical method for the calculations of the electric field and current density in needle-ring configurations of the corona discharge plasma. The needle is considered hyperboloidal. The result of the calculation indicates that the strong electric field generated by the sharp needle due to the unsymmetrical of geometric form of it. Similarly, the current density at the tip of the sharp needle is higher than at the ring. The value of the electric field and current density depends on geometric form, especially radius of the hyperbola, radius of the ring, and the distance between the electrodes. Radius of the hyperbola must be smaller than radius of the ring.   Keywords : electric field, current density, needle-ring configurations, geometric form
Analisis Fusan Hasil Fusi Protoplas Intraspesies Pichia manshurica DUCC-015 (Analysis of Fusant from Protoplast Fusion Intraspecies of Pichia manshurica DUCC-015) Roslenawati, Roslenawati; Kusumaningrum, Hermin Pancasakti; Pujiyanto, Sri
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 1 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

The intraspecies protoplast fusion of P. manshurica DUCC-015 was conducted in searching the fusant with greater inulinase production. Inulinase on Dahlia variabilis Willd tuber from Baturraden-Purwokerto showed inulinase activity 0,683 IU/mL. Inulinase is an enzyme that catalyzes the hydrolysis reaction of inulin polysaccharides into fructose and or fructooligosacarides. The aims of this research was revealing fusant from intraspecies protoplast fusion process of P. manshurica DUCC-015 followed by analyzing of their inulinase productivity and activity. The research metodology protoplast fusion process consist of lysis of cell wall, protoplast fusion and regeneration of intraspecies fusant P. manshurica DUCC-015. Analysis of fusant were done by Schiff Basic Fuchsin staining of fusant nuclei, also measuring the inulinase activity and inulinase production comparing with their parent. The inulinase activity will be analyzed by T-Test Independent Two Sample on 95% Confidence interval of the difference use Statistical Product and Service Solution programme (SPSS). The result of experiment gaining fusant with regeneration capability, ploidi diversity  of fusant, inulinase activity about 0,965 IU/mL while their parent 0,622 IU/mL and inulinase production 0,736  IU/mL comparing with 0,731 IU/mL during 42 hours incubations. The fusant indicated the increase of inulinase activity and production  compared with their parent.   Keywords: Pichia manshurica DUCC-015, fusant, inulinase    Fusi protoplas intraspesies Pichia manshurica DUCC-015 telah dilakukan untuk mencari fusan dengan produksi inulinase yang lebih tinggi. Inulinase pada umbi tanaman Dahlia variabilis Willd dari Baturraden-Purwokerto memperlihatkan aktivitas sebesar 0,683 IU/mL. Inulinase merupakan enzim yang mengkatalisis reaksi hidrolisis polisakarida inulin menjadi fruktosa dan atau fruktooligosakarida. Penelitian ini bertujuan untuk mendapatkan fusan hasil fusi protoplas intraspesies P. manshurica DUCC-015 yang memiliki aktivitas inulinase lebih tinggi. Rancangan percobaan fusi protoplas terdiri dari isolasi protoplas, fusi protoplas dan regenerasi fusan. Analisis fusan menggunakan pewarnaan  Fuchsin pada inti sel, mengukur aktivitas dan produksi inulinase fusan. Aktivitas inulinase dianalisis dengan Uji T Test Dua Sampel Independen pada taraf kepercayaan 95% menggunakan program Statistical Product and Service Solution (SPSS). Hasil penelitian menunjukkan fusi protoplas intraspesies P. manshurica     DUCC-015 menghasilkan aktivitas inulinase mencapai 0,965 IU/mL dibandingkan induk sebesar 0,622 IU/mL dan produksi inulinase 0,736  IU/mL pada inkubasi selama 42 jam. Fusan  mengindikasikan kenaikan aktivitas dan roduksi inulinasi dibandingkan induk. Kata kunci : Pichia manshurica DUCC-015, fusan, inulinase.
Efek Hidrolisis Ekstrak Daun Iler (Coleus scutellarioides) Terhadap Aktivitas Inhibisi Enzim α-glukosidase Auliawan, Riky; Cahyono, Bambang
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 1 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

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Abstract

Penemuan antidiabet dari suatu senyawa bioaktif atau ekstrak tanaman banyak diarahkan pada mekanisme penghambatan hidrolisis ikatan glikosidis, dan umumnya tertuju pada senyawa fenolik. Tanaman iler (Coleus scutellarioides), yang secara tradisional telah digunakan untuk antidiabetes, dilaporkan mengandung senyawa dari golongan tersebut. Penelitian yang bertujuan membandingkan  aktivitas dari ekstrak daun iler dan ekstrak yang terhidrolisis telah dilakukan dalam riset ini. Penyediaan ekstrak dilakukan melalui maserasi dalam pelarut etanol, dan hidrolisis ekstrak dilakukan dalam suasana asam (HCl 2N). Ekstrak dan hasil hidrolisis dianalisis fenolat total dan aktivitas penghambatan enzim alfa-glukosidase.  Hasil penelitian menunjukkan bahwa ekstrak etanolat daun iler dapat diperoleh dengan rendemen 4,50%. Adanya peningkatan kadar fenolat total rata-rata dari ekstrak setelah dihidrolisis sebesar 23,99 mg GAE/g bobot sampel kering (dari 156,38±0,25 menjadi 180,37±0,260 dapat mengindikasikan bahwa ikatan glikosida pada ekstrak asal telah terhidrolisis oleh HCl. Uji aktivitas penghambatan a-glukosidase terhadap sampel sebelum dan sesudah hidrolisis menunjukkan harga IC50 sebesar 735,67±11,51µg/mL dan 724,33±8,60µg/mL. Secara statistik, nilai aktivitas kedua bahan ini tidak berbeda secara signifikan. Hasil penelitian ini dapat membuktikan bahwa ikatan  glikosida dalam senyawa organik ekstrak daun iler tidak memberikan efek terhadap peningkatan aktivitas penghambatan enzim glukosidase. Kata kunci: Coleus scutellarioides, daun iler, fenolat total, enzim α-glukosidase, hidrolisis ekstrak    The studies of antidiabetic activity of a bioactive compound or plant extract have been directed into the inhibition mechanism of glycoside bond hydrolyses, and generally focused on phenolic compounds. Iler plants (Coleus Scuterllariousdes) that traditionally used as antidiabetic, has been reported containing those compounds. Aim of this study is comparing the activity of Iler leaves extract and their hydrolyzed extracts. The extracts were prepared by ethanol maceration and acid hydrolysis (HCl 2N). Both ethanol and hydrolyzed extracts were analyzed for total phenolic and alpha-glucosidase inhibitory activity. The result showed that the extract yield was 4.50%. The increasing number of total phenolic content after hydrolysis (23.99 mg GAE/g weight of dry samples) indicated that the glycoside bonds in extracts have been hydrolized by HCl. The alpha-glucosidase inhibitory (IC50) of ethanol and  hydrolyzed extracts were 735.67±11.51µg/mL dan 724.33±8.60µg/mL respectively. Statistically, the differences of inhibitory activity of both extracts was not significant. This result proved that the glycoside bond in Iler leaves extract does not enhance the inhibition activity of glucosidase enzyme. Key-words : Coleus scutellarioides, total phenolics, α-glucosidase enzyme, extract hydrolysis

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