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Biota
Published by Universitas Islam Negeri Mataram
ISSN : 20863586     EISSN : 24608483     DOI : -
Core Subject : Health, Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Medicine & Pharmacology
Focus and Scope of Biota on Bioscience, includes: cell biology and genetics, biodiversity, ecology, physiology and biotechnology. Biota covers all life forms, including microbes, fungi, plants, virus, animal and human. Focus and Scope of Biota on Biology Education, includes: Experimental Research, Class Action Research, Qualitative Research, Quantitative Research, Model Learning Development, Media Learning Development, and Learning Evaluation.
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Articles 6 Documents
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Search results for , issue "Vol 16 No 1 (2023)" : 6 Documents clear
The Genetic Diversity of Macroalgae on The Ekas Coast of East Lombok Based on Chromatogram Data from PCR Ampification Baiq Repika Nurul Furqan; Imam Syahputra Yamin; Sri Sofiati Umami
Biota Vol 16 No 1 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i1.417

Abstract

Macroalgae are multicellular photosynthetic organisms that live in water. Some species of macroalgae are very difficult to identify based on their morphology alone because they have great similarities. Therefore, identification of macroalgae diversity based on its genotype is very important to do in order to enrich the genetic information of macroalgae found on one of the beaches on the island of Lombok. This study aimed to analyze the genetic diversity of macroalgae from Pantai Ekas Beach, Jeowaru District, East Lombok, NTB using 18S rRNA gene markers. The research was carried out in 4 stages, namely sampling, morphological analysis of samples, isolation of total macroalgae DNA, isolation of genes by PCR amplification, agarose gel electrophoresis. Sampling was carried out at certain coordinates at east longitude and south latitude using the Ekas Beach quadratic transect method. The morphological analysis of the samples was guided by an identification key. Total DNA, macroalgae were isolated based on the spin column I DNA kit easy method. Genes were isolated by means of PCR amplification. Total DNA isolates and gene amplicons were confirmed by agarose gel electrophoresis. The results obtained were based on their morphology, all samples obtained were grouped into 9 macroalgae samples which could be divided into brown macroalgae (3 samples), green macroalgae (2 samples) and red macroalgae (4 samples). All samples have successfully isolated total DNA and its 18S rRNA gene which was confirmed by agarose gel electrophoresis chromatogram.
Inggris Subhan Maulidi Kun Bad'a Farohii; Fatchur Rohman; Sulisetijono Sulisetijono
Biota Vol 16 No 1 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i1.420

Abstract

Research on the characterization of potential perennials to resist river erosion in the Brantas river border based on different environmental features in Malang, East Java has been carried out. The research aims to know the composition of plant species, plant diversity, and the dominance of perennials that can prevent erosion. The study used vegetation analysis with cruise methods, exploring perennials on the Brantas river border based on different environmental settings (residential industry, environment, fields). The results of the study found 49 species of plants in 25 families, the diversity of hard plants in the residential environment was high with H' 3.06 while in the industrial and rice fields/fields environment it was moderate with H' 2.67 (Industry) and H' 1.90 (fields). The dominant plants are Gigantochloa apus (Schult. f.) Kurz ex Munro (Industry) species, Calliandra houstoniana (Mill.) Standl. (Settlement) and Pinus merkusii Jungh. & Vriese ex Vriese. (fields) Many perennials in the study area can prevent erosion because these plants meet the criteria of a strong root system and economic value. Keywords: Perennials, Erosion, Border, Brantas River
Optimization of Biodiesel Production from Sludge of Mask Waste and Used Cooking Oil Using Lipase as Biocatalyst Muhammad Rizal Januardi; Baiq Repika Nurul Furqan
Biota Vol 16 No 1 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i1.423

Abstract

Mask waste and used cooking oil contain hydrocarbons and free fatty acids. the combination of these ingredients with pretreatment heating in the microwave and the help of lipase enzymes makes the resulting sludge has the potential to be converted into biodiesel with a maximum yield when compared to making biodiesel from other materials and methods. The results of this study indicate that pre-treated sludge from mask waste and used cooking oil can be converted into biodiesel through a methanol transesterification reaction with the help of thermostable lipase enzymes from Pseudomonas. As for the optimum conditions, the highest biodiesel yield was produced at a temperature of 60℃, the best reaction time was 25 minutes, the volume of sludge was 10 mL and the optimum pH was at pH 8. The flame test on the biodiesel produced had been carried out, most of which had bright blue, red flames and orange. There are also some results that the flame is dim and even the test results are not lit.
Identifikasi Ragam Jenis Jamur di Taman Buah Lokal Mekar Lestari Candra Pratama; Amalia Rezeki; Ferry F. Hoesain
Biota Vol 16 No 1 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i1.428

Abstract

Mekar Lestari Local Fruit Park is a park managed by the Indonesian Center for Biodiversity Studies and Conservation. This park has a collection of local fruit plants located in Anjir Muara, Barito Kuala Regency. One of the potential organisms found in this area is fungi. The purpose of this study was to identify the types of fungi in the local fruit garden area "Mekar Lestari" using the exploration method with the selected sample technique. The data obtained were then analyzed by descriptive method. Based on the results of research that has been carried out in the forest in the local fruit garden area "Mekar Lestari" found 15 species of fungi namely Arcyria cinerea, Calocera cornea, Daldinia concentrica, Marasmius siccus, Physarum polycephalum, Physarum album, Volvariella sp, Trametes sanguine, Trametes ochracea, Schizophyllum commune, Collybia cookie, Xylaria polymorpha, Scleroderma cepa, Auricularia sp, and Ductifera pululahuana. The types of fungi found in this study are divided into 12 families and 13 genera. The environmental conditions of the study site were temperature 30.2-37.7 °C, air humidity 77.0-84.8%, light intensity 198-2460 lux and soil pH 6.3-7. The fungi found generally live on decaying wood and litter, and a small proportion live attached to trees
"Bau Nyale " from the perspective of Science, Local Wisdom and Ecotourism Ramdhani Sucilestari; Agus Ramdani; Susilawati Susilawati; AA Sukarso; Joni Rokhmat
Biota Vol 16 No 1 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i1.459

Abstract

Bau Nyale is an annual tradition of the Sasak people in Central Lombok, which is celebrated every year on the 20th of the 10th month according to the Sasak calendar. This tradition comes from a legend that lives in society about the sacrifice of a princess to maintain the peace and unity of her country. Through a multidisciplinary approach that combines the perspectives of science and ecotourism sustainability, this study aims to present a comprehensive understanding of the Nyale legend and phenomenon and their associated ecotourism implications. This research method includes a literature study, field observations and interviews. The results of this study provide a deeper understanding of the legend of Princess Mandalika, the Bau Nyale phenomenon, and the related ecotourism implications. It was found that Nyale rituals have significant ecological and social impacts, including potential reductions in Nyale sea worm populations and changes to local culture. Therefore, sustainable ecotourism management is needed to ensure the sustainability of this phenomenon in the long term. This research has important implications in cultural preservation and environmental conservation. It is hoped that this study can contribute to an understanding of the interaction between cultural traditions and ecotourism and become a basis for developing sustainable management strategies in the context of the Bau Nyale phenomenon.
Endophytic Bacteria Bengkulu's Coffee and Their Prospects Dadan Supardan; Yosi Yulizah; Fatkhanuddin Aziz; Fauziah Fitriana
Biota Vol 16 No 1 (2023)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v16i1.461

Abstract

Antimicrobial resistance (A.M.R.) is one of the main threats in the health sector. Most antimicrobials, including antibiotics, antifungals, antivirals, and antiparasitics, are derived from bioactive compounds found in bacteria, particularly the actinomycetes group. This study aimed to isolate and test endophytic bacteria on coffee plants against antibiotic-resistant pathogenic bacteria, namely penicillin-resistant S. aureus. This study used several methods: endophytic bacteria isolation, bacterial purification, gram staining, and endophytic bacteria antagonist test against antibiotic-resistant bacteria. Eight endophytic coffee isolates were obtained from the roots and pods of Robusta coffee. One bacterial isolate with the code AR4 had an antagonistic effect on tetracycline-resistant S. aureus bacteria.

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