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INDONESIA
Teknologi Indonesia
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 01261533     EISSN : 23031913     DOI : -
Core Subject : Science,
Computer Science & IT
JTI is a journal in the Departement of Engineering Sciences - Indonesian Institute of Sciences (LIPI). JTI has policy to publish a new and original research paper or a review paper in The scope of Technology. JTI publishes two issues per year. The journal has been registered with printed-ISSN number as 0126-1533 and electronic ISSN number as 2303-1913.
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue " Vol 32, No 2 (2009)" : 8 Documents clear
PURIFICATION AND CHARACTERIZATION OF WHITE RADISH (RAPHANUS SATIVUS L.VAR LONG WHITE) PEROXIDASE FROM CELL CULTURE EXTRACT Pudjiraharti, -; Karossi, A.T.
Teknologi Indonesia Vol 32, No 2 (2009)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v32i2.8

Abstract

Horseradish peroxidase (HRP) has been widely used as a component of clinical diagnostic reagent for Enzyme Linked I mmunosorbent Assay (ELISA) technique. White radish as one of Brassicaceae family members was found to have high peroxidase activity. In this study, white radish (Raphanus sativus L. var Long White) was used for the production of peroxidase by cell suspension culture technique. Isolation of the enzyme was conducted by ammonium sulfate precipitation followed by purification using DEAE-Cellulose column chromatography eluted with 0.01 M phosphate buffer, pH 7.5 and 0-0.5 M NaCl gradient. A major peak of protein having the highest enzyme activity and purity 25 folds compared to the crude was resulted. SDS-Polyacrilamide gel electrophoresis showed one main band with molecular weight of 47.000 Da. This white radish peroxidase enzyme is very efficient by demonstrating maximum activity at temperature 55oC and pH 7.5 as well as a Km 76.6 ?g/mL and V max 275 ?g/mL/minute toward hydrogen peroxide substrate and pyrogallol hydrogen donor.
Molecular Conformation and Thermal Diffusivity by Temperature Wave Analysis of Electric Polarized Biaxially Stretched Polyethylene Terephthalate Films Satoto, Rahmat; Nugroho, Pramono
Teknologi Indonesia Vol 32, No 2 (2009)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v32i2.4

Abstract

Structural and conformation of biaxially polyethylene terephthalate (PET) films after being polarized by electricfield was characterized using Fourier transform infrared (FTIR). The 950 kV/cm electric field was applied during the sample heating and cooling at 28-100-28oC. The electric field was directed to the sample thickness. Thermal diffusivity of the polarized PET films in the thickness direction was characterized using temperature wave analysis (TWA) as a function of temperature. An orientation function of 1719 cm-1 band which was assigned to a permanent dipole of C=O, shows an increasing orientation with the increase of polarization field. Thermal diffusivity also increased with the increasing of electric polarization field. The orientation function of 1340, 971 and 847 cm-1 bands did not show a clear tendency due to the polarization field. The correlation of molecule conformation change and thermal diffusivity in the biaxially stretched PET films is discussed.
99 PEMBUATAN BAHAN FILTER KERAMIK BERPORI BERBASIS ZEOLIT ALAM DAN ARANG SEKAM PADI Sebayang, P.; Muljadi, -; Tetuko, Anggito P.
Teknologi Indonesia Vol 32, No 2 (2009)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v32i2.9

Abstract

The porous ceramics filter material have been made from natural zeolit and rice husk charcoal with composition variation (in % of mass). The preparation of zeolit was done using ball mill for 24 hours and rice husk charcoal was prepared using vibrating mill for 1 hour until it can be through out in 100 mesh shieve. The two raw materials about 2 grams were mixed with addition 50 ml of Polivinylalcohol (PVA), then it was mixed again until homogeny. The sample was dried pressed with pressure of 25 kgf/cm and dried in a drying oven at 60 C for 24 hours. The sintering process was done using electrical furnace with heating rate of 10C/minute until it reached temperature of 900 and 1000 C, and holded for 2 hours. From the result of porouc ceramics filter material, the optimum composition is 40% rice husk coke + 60% Zeolit with sintering temperature = 1000 C. At this condition, the properties are density = 2,16 g/cm, shrinkage = 35,94%, porosity = 66,05%, water absorption = 31,10%, modulus of rupture = 7,47 MPa, compressive strength = 4,38 MPa, and coefficient of thermal expansion = 5 x 10 -6 C-1. The result of XRD shows that zeolit has major phase of mordenite (Na8[Al8Si40O96].24H2O) and minor phase of clinoptilolite (Na6[Al6Si36O72].24H2O). The result of XRD on 40% rice husk coke + 60% Zeolit and sintering temperature = 1000 C shows that the major phase is mullite (3Al2O3SiO2) and the minor phase is tridimite (SiO2). Meanwhile, the picture of SEM shows that porous ceramics have irregular particle with size between 110 ?m and its pore size can reaches 20 ?m.
Effect of Electric Polarization on Thermal Diffusivity of Biaxially Stretched Poly (ethylene terephthalate) Films Satoto, Rahmat
Teknologi Indonesia Vol 32, No 2 (2009)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v32i2.5

Abstract

The effect of the electric field polarization on the thermal diffusivity of biaxially stretched poly (ethylene terephthalate) (PET) films had been examined. The change in the thermal diffusivity as a function of temperature was measured by temperature wave analysis (TWA) technique. The thermal diffusivity at room temperature increases with the increasing of the polarization electric field. The thermal diffusivity decreases with the increasing of the temperature. The decrease of thermal diffusivity by the increase of temperature could be caused by the effect of carbonyl depolarization. The glass transition temperature, Tg, decreased with the increasing of the polarization electric field. The increase of thermal diffusivity is due to the increase of carbonyl polarization of the PET. Segmental orientation of carbonyl in the thickness direction played an important role in the thermal diffusivity. These results implied that the temperature dependence of the thermal diffusivity was related to the change of the dipolar relaxation and microstructure of PET.
PEMBUATAN NANOPAR TIKEL LixMn2O4 Wigayati, Etty Marti
Teknologi Indonesia Vol 32, No 2 (2009)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v32i2.10

Abstract

LixMn2O4 nanoparticle has been prepared using Li2CO3 and MnO2 as starting materials. Firstly, Li2CO3 and MnO2 were mixed using solid state reaction, then the powder was calcinated at 700oC and sintered at 800oC. The resulted pouder was characterized using SEM, XRD, and EIS (Electrochemical Impedance Spectroscopy). Henceforth, LixMn2O4 powder was processed into nanoparticle size through milling process using PBM (Planetary Ball Milling) for 80 hour and ultrasonificator with power of 300 watt for 2 hour. The characterization was conducted again using SEM, XRD, PSA and EIS. The result shows that there is no diffraction pattern change after the milling process. Examination using SEM shows particle changes from needles to round shapes with smaller sizes. Diffraction analyzes using Rietveld method results in lattice parameter of 8.23454 , cell unit volume of 558.3647 3 and the crystal structure is cubic with space group of Fd-3m. The particle size of the final product is analyzed using Particle Size Analyzer (PSA) which results in the value of 178 nm in average diameter. The measurement of electrical impedance gives values of 2 M ohm (before milling) and 8 M ohm (after milling).
Biodegradasi Limbah Padat Organik secara anaerob: Proses semi kontinu Sriwuryandari, Lies; Sembiring, Tarzan
Teknologi Indonesia Vol 32, No 2 (2009)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.042 KB) | DOI: 10.14203/jti.v32i2.6

Abstract

On the minimizing of organic solid waste coming to open dumping area, the municipal organic waste was used as substrate for anaerobic degradation and methane generation. Experiment either in the semi continuous bioreactor or in serum vials showed that the rate of organic substance conversion is low. The degradation rate is between 3550 % of organic material feed into bioreactor where pH is in optimum condition for methane generation. The overall limiting step of organic material biodegradation is the hydrolysis of organic fiber into soluble organic substance.
VARIASI SUHU MUKA LAUT BULANAN BERDASARKAN KANDUNGAN KIMIA KORAL Cahyarini, Sri Yudawati; Pfeiffer, Miriam; Prayudi, Dudi
Teknologi Indonesia Vol 32, No 2 (2009)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.045 KB) | DOI: 10.14203/jti.v32i2.11

Abstract

Information on environmental changes which is available today is limited to only dozen years back. In order to make an assesment on environmental changes, information on longer time is required. Corals can provide environmental data till thousand years back. To have some information, it is required to analyze geochemical proxy. By analyzing geochemical elements such as Sr/Ca, ?18O, sea surface temperature and salinity can be reconstructed. Further, by analyzing and interpreting time series data of coral geochemical proxy can be used to understand intensity, process, and mechanism of the environmental damage. In this study, reconstruction of sea surface temperature using Sr/Ca and ?18O coral from Timor is used as a case. The result shows that Sr/Ca is influenced by sea surface temperature.
SAGO STARCH AS FERMENTATION MEDIUM FOR PRODUCTION OF AND SUBSTRATE FOR CARBOHYDRATE ENZYMES Karossi, A.T.
Teknologi Indonesia Vol 32, No 2 (2009)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.042 KB) | DOI: 10.14203/jti.v32i2.7

Abstract

It is well known that sago starch extracted from sago palm (Metroxylon sp.) is abundantly available especially in eastern part of Indonesia. On the other hand, amylases and related enzymes are recognized as the most important industrial enzymes, specifically in the field of food industries. Therefore, fermentation experiments were carried out to utilize sago starch as medium component to produce extracellular alpha amylase and glucoamylase as well as glucose isomerase, which is the later being an intracellular enzyme. Aspergillus oryzae was selected fermenting microorganism to produce the extracellular alpha amylase and intracellular glucose isomerase, while Rhizopus oryzae was for producing extracellular glucoamylase. The investigation included optimum fermentation process condition for enzymes production, both in Ehrlenmeyer shaken flasks and stirred tank fermentors, then was followed by isolation with ammonium sulfate fractionation, concentration employing polysulfon membrane, purification with chromatography technique, and characterization of the enzymes. In fact, sago starch could function as a medium component in fermentation producing amylases as well as a substrate for the enzymes. The biomass of the Aspergillus contained glucose isomerase activity. Experiments with fermentor indicated that alpha amylase was best produced at agitation rate of 500 rpm, while glucoamylase was at 350 rpm, both at 1.5 vvm aeration. The SDS-PAGE of the purified enzymes found that the alpha amylase and glucoamylase had molecular weights of 53 and 36 kDalton respectively, while the glucose isomerase indicated to have two subunits with molecular weight each of 55 and 60 kDalton. As a substrate for alpha amylase and glucoamylase, sago starch exhibited higher affinity compared with arenga, corn, and cassava starches although it was still inferior to soluble starch.

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