cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kab. banyumas,
Jawa tengah
INDONESIA
Scripta Biologica
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 23553138     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.
Arjuna Subject : -
Articles 13 Documents
 1   2 
Search results for , issue "Vol 4, No 2 (2017)" : 13 Documents clear
ANALISIS BIOMASA DAN CADANGAN KARBON PADA BERBAGAI UMUR TEGAKAN DAMAR (Agathis dammara (Lamb.) Rich.) DI KPH BANYUMAS TIMUR Zinatul Uthbah; Eming Sudiana; Edy Yani
Scripta Biologica Vol 4, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (845.798 KB) | DOI: 10.20884/1.sb.2017.4.2.404

Abstract

Increased carbon dioxide in the atmosphere causes global climate change seriously. Forests serve as an important asset that can absorb and store carbon in the form of biomass. One type of potential forest as a carbon sink is forests resin. The amount of carbon stored by standing very dynamic and varies according to the age of its standing. Therefore, studies will be needed to determine the effect of age on biomass and carbon stocks stands resin, determine the relationship between age and standing biomass and carbon stocks resin, and knowing the optimum resin stand age in storing biomass and carbon stocks. This research was conducted at the stands of resin RPH Karang Gandul, KPH Banyumas Timur for four weeks in May 2016. The method used is a survey with a sampling technique using cluster random sampling. Stands resin used in the study were classified into five age groups with 5 replicates. Data were analyzed using ANOVA with an error rate of 5% and continued with LSD for further test results were significant and regression analysis to determine the relationship of age with biomass and carbon stocks stands resin. The results showed that the age effect on biomass and carbon stocks stands resin, the relationship formed between the age of stand with biomass and carbon stocks are quadratic, and age optimum in storing biomass and carbon stocks is 35 years.
METODE EKSTRAKSI DNA CABAI (Capsicum annuum L.) MENGGUNAKAN MODIFIKASI BUFFER CTAB (CETHYL TRIMETHYL AMMONIUM BROMIDE) TANPA NITROGEN CAIR Kristianto Nugroho; Rerenstradika T Terryana; Puji Lestari
Scripta Biologica Vol 4, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (747.778 KB) | DOI: 10.20884/1.sb.2017.4.2.423

Abstract

Chili pepper is an agricultural commodity having high economic value. The production and supply of chili pepper frequently did not match the increased demand; it caused the market price fluctuated. It is important to create new varieties of chili pepper with high production trait to overcome the scarcity. Therefore the plant breeding activities for chili pepper should be done intensively in both conventional and molecular-based to obtain varieties of chili pepper with expected qualities. In molecular breeding, DNA extraction is the crucial steps of the process. If extracted DNA has an excellent quality and quantity,  the next processes normally could be completed with the high-quality result. To date, most methods of DNA extraction used liquid nitrogen to destroy the tough carbohydrates of plant tissue. Liquid nitrogen is nitrogen gas in a fluid state which quite difficult to be distributed to the remote laboratory wit no available storage facility. This study aimed to obtain a modified DNA extraction method, in particular for chili pepper, which capable to produce DNA with high quality and quantity without using liquid nitrogen. The sample used consisted of eight F2 plants including their hybrid-parental of the Kencana and the 0207. This research applied modified Doyle and Doyle method for extraction. Modification of extraction buffer is done through the addition of the 1% (w/v) PVP (Polyvinylpyrrolidone) and 0.2% (v/v) β-mercaptoethanol. The results showed that the DNA extracted using this method has good quality and quantity, capable of being amplified by using SSR (Simple Sequence Repeat) primer and could be digested by restriction enzyme EcoRI. Besides, this method can reduce dependence on the use of liquid nitrogen, in particular for remote laboratories with no available storage facility.
PETUNJUK PENULIS dan DAFTAR ISI Romanus Edy Prabowo
Scripta Biologica Vol 4, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (875.62 KB)

Abstract

Page 2 of 2 | Total Record : 13

 1   2 

Filter by Year

2017 2017


Reset
Filter By Issues
All Issue Vol 10, No 3 (2023) Vol 10, No 2 (2023) Vol 10, No 1 (2023) Vol 9, No 2 (2022) Vol 9, No 1 (2022) Vol 6, No 4 (2019) Vol 6, No 3 (2019) Vol 6, No 2 (2019) Vol 6, No 1 (2019) Vol 5, No 4 (2018) Vol 5, No 3 (2018) Vol 5, No 2 (2018) Vol 5, No 1 (2018) Vol 4, No 4 (2017) Vol 4, No 3 (2017) Vol 4, No 2 (2017) Vol 4, No 1 (2017) Vol 3, No 4 (2016) Vol 1, No 4 (2014) Vol 1, No 3 (2014) Vol 1, No 2 (2014) Vol 1, No 1 (2014) More Issue