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Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
Published by Universitas Lampung
ISSN : 23384344     EISSN : 2686200X     DOI : https://doi.org/10.23960/jbekh.vxxx.xxx
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
This journal focuses on the fields of Agricultural, Animal Sciences, Bioconservation, Biopharmacology, Biotechnology, Biomedical, Biological control, Behavioural ecology, Plant Sciences, Ecology, Fishery, Marine Biology, Fresh Water Biology, Microbiology, Immunobiology, Ecotoxicology, and Parasitology. This journal utilizes both the LOCKSS and CLOCKSS systems to create a distributed archiving system among participating libraries and permits those libraries to create permanent archives of the journal for purposes of preservation and restoration
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 10 Documents
 1 
Search results for , issue "Vol. 8 No. 2 (2021)" : 10 Documents clear
THE AIR QUALITY AND NOISE STUDY IN SETTLEMENTS AND METAL SCRAP MELTING FACTORY Wahid Giantara; Tugiyono Tugiyono; Agus Setiawan; Gregorius Nugroho Susanto
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.159

Abstract

Air is an important component for the survival of living things, especially humans. However, air quality has decreased due to pollution. The harmful elements that enter the atmosphere are Carbon Monoxide (CO), Nitrogen Dioxide (NO2), Sulfur Dioxide (SO2), Particulates (PM10), and Ozone (O3). Noise is defined as unwanted sound or sound so that it disturbs and endangers health. Sources of noise are divided into moving and stationary noise sources. The method used in this research is grab sampling by installing an air sampler impinger to capture particles or pollutants as well as taking noise data using a simple method with a Sound Level Meter with a reading every 5 seconds within 10 minutes for one measurement. The results showed that the air quality in residential and workspace areas was still within quality standards. The noise level in residential areas is still acceptable, but the noise level in the production room surpasses the acceptable limit.
PRODUCTION OF AMYLOCELLULOLYTIC ENZYMES AND THEIR VIABILITY ON CARRIER MEDIA BY Bacillus sp. U4 AND Pseudomonas sp. U3 Enny Zulaika; Salma Shavira Rahma Khofifah
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.186

Abstract

Amylase and cellulase are enzymes that are amyocellulolytic. Both are extracellular enzymes that can degrade organic materials, namely starch and cellulose. The purpose of this study was to determine whether Bacillus sp. U4 and Pseudomonas sp. U3 could produce amylocellulolytic enzymes and determine their viability after being incubated for 2 hours on the husk, peat, and sawdust carrier media. Screening for the presence of amylase was carried out on a selective medium, namely nutrient agar-amylum 0.5 %, and cellulase on carboxymethyl cellulose-agar medium. Both isolates were inoculated into sawdust, husk, peat carrier media and incubated for 2 hours. Viability was observed using the total plate count method. Bacillus sp. U4 and Pseudomonas sp. U3 can produce amylase and cellulase. The best isolate viability after incubation for 2 hours was found in husk carrier media with 2.13 x 105 CFU/gr of husk media.
THE EFFECTIVENESS OF PEPPER LEAVES (Piper nigrum L.) AS A LARVACIDE OF Aedes aegypti MOSQUITO Yuyun Solihat; Emantis Rosa; Gina Dania Pratami; Nuning Nurcahyani
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.187

Abstract

Dengue Hemorrhagic Fever (DHF) cases are quite high in Indonesia. One of the causes of high dengue cases is the high vector population, namely the Aedes aegypti mosquito. Mosquito control Ae. aegypti one of them uses larvicides to break the chain of reproduction. The use of chemical-based larvicides can be bad because it can pollute the environment and cause resistance. As an alternative, you can use natural larvicides made from plants. One of the plants that have the potential as larvicides are pepper (Piper nigrum L.) because it contains compounds that can be toxic to insects such as saponins, flavonoids, alkaloids, and essential oils. In addition, pepper plants are widely cultivated in Lampung and are easy to obtain. The purpose of this study was to determine the effectiveness of pepper leaf (P. nigrum L.) as larvicide for Ae. aegypti. This study used a completely randomized design (CRD). There was 1 control and 5 treatments in the form of pepper leaf extract with concentrations of 0.2%, 0.4%, 0.6%, 0.8%, and 1%. The test material used was Ae. aegypti instar III. Each treatment used 25 larvae with 4 repetitions. Observations were made after 24 hours of treatment. The data obtained in the form of the number of larval deaths were analyzed using one-way ANOVA and Post hoc LSD test. The results of this study showed a significant difference (p = 0.000), and it was found that a concentration of 1% was the most effective concentration as a larvicide for Ae. aegypti with a larval mortality percentage of 92%.
BIOACCUMULATION OF IRON (Fe) IN Bacillus JA1, Sporosarcina JA4, AND Lysinibacillus JB2 Afianita Dian Rahmawati; Enny Zulaika
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.188

Abstract

Carbonatogenic bacteria are able to produce calcium carbonate (CaCO3). Steel reinforcement of concrete is mainly made of a mixture of carbon (C) and ferrous (Fe) elements. Fe is classified as heavy metal, when in high concentrations it is toxic to bacteria. The purpose of this study was to determine the bioaccumulation of carbonatogenic bacteria to Fe. The isolates used in this study were Bacillus JA1, Sporosarcina JA4, and Lysinibacillus JB2. All isolates were subcultured on nutrient agar slant media. Cultures were made on minimal salt medium: nutrient broth (95% : 5%) with 24 hours incubation. Bioaccumulation test using minimal salt medium: nutrient broth (95% : 5%) containing ferrous 5 ppm and 10 ppm. Accumulated ferrous concentration was measured using atomic absorption spectrophotometry. The result of this research is that the bioaccumulation of ferrous by the isolates after 2 hours of incubation is greater than 1 hour of incubation. Bacillus JA1 has the highest bioaccumulation ability which was 9,19 ppm after 2 hours exposure of 10 ppm ferrous and its ferrous bioaccumulation efficiency was 91,85%.
THE RESISTANCE OF CHILLI (Capsicum annuum L.) FROM SEEDS INDUCED BY 0.2 mT MAGNETIC FIELD AND INFECTED BY Fusarium oxysporum Feni Kaisah; Rochmah Agustrina; Eti Ernawiati; Martha L. Lande; Lili Chrisnawati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.191

Abstract

Red chili pepper (Capsicum annuum L.) is one of the most widely cultivated commodities in Indonesia. However, until now the production was never covering the public’s demand. Red chili pepper plants are susceptible to disease caused by the infection of Fusarium oxyporum (Fox) fungi, causing a decrease in plants’ production. Fungicide usage to control Fox can cause plants’ resistance to the pathogen. The eco-friendly way to control Fox could be achieved by utilizing a magnetic field. This research arranged using Completely Randomized Design (CRD) with the combination between 0,2 mT magnetic field induction and Fox infection consist of control (M0); 0,2 mT magnetic field induction for 7 minutes and 48 seconds (M7); and 15 minutes and 36 minutes (M15); as well as seed infection with Fusarium oxyporum that consist of control without infection (F0) and infected for 60 minutes (F60). Each test unit was repeated 5 times.   
EVALUATION OF WATER QUALITY IN ANIMAL FEED FACTORY ENVIRONMENT BASED ON PLANKTON AND BENTHOS BIOINDICATORS Feni Yulinda; Tugiyono; Agus Setiawan; Elly Lestari Rustiati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.192

Abstract

Water is a source of life that plays an important role for all living things. Rivers are one of the water sources that are vulnerable to pollution. This study aimed to evaluate the water quality of the Way Napal river based on the diversity of plankton and benthos. The research was conducted from November 2020 to March 2021. Sampling and analysis were performed by field technicians from the SEAMEO BIOTROP Environmental Laboratory. Plankton diversity was calculated using the Shannon Weaver diversity index. The results showed that the phytoplankton diversity index at AP-1, AP-2, and AP-3 locations ranged between 2,080-2,278 these values had moderate biota stability and were included in the uncontaminated category. The zooplankton diversity index at the AP-1 location has a value of 1,119 with moderate biota stability and is included in the moderate category, while at the AP-2 and AP-3 locations it has a value of 0.925-0.995 with unstable biota diversity and is included in the highly polluted category. The Benthos diversity index ranges from 1.343 - 1.772 with moderate biota stability and is included in the moderately polluted category.
THE DYNAMICS OF BRYOPHYTES SPECIES DIVERSITY IN THE LOWLAND ECOSYSTEMS, CIBINONG SCIENCE CENTER-BOTANICAL GARDEN Fandri Sofiana Fastanti; Tri Yuni Indah Wulansari
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.194

Abstract

Bryophytes are a group of plants generally with small sizes and spores as generative reproduction. There are three main groups of Bryophytes: liverworts or hepatics (Marchantiophyta), mosses (Bryophyta), and hornworts (Anthocerophyta). They are found in almost all ecosystems. Ecopark Cibinong Science Center-Botanical Garden (CSC-BG) is a lowland ecosystem protection area located in industrial cities. The bryophytes species that were carried out in 2009 and 2010 are inadequate. Re-exploration activity was carried out from June 2018 until January 2019 in all blocks of Ecopark. This study aims to report an update of bryophytes data in Ecopark. A total of 20 species of bryophytes were found in this research. Fissidens is the most common genus of bryophytes. There are 11 species of bryophytes and 9 species are new data records for Ecopark CSC-BG. This study updated bryophyte diversity data from Ecopark CSC-BG in 2009 and 2010, resulting in the discovery of 30 species (27 species mosses, 3 species liverworts), including 10 species not found in previous studies. There are no hornworts found in Ecopark CSC-BG.
THE ARACEAE OF ITB JATINANGOR CAMPUS, SUMEDANG, WEST JAVA Arifin Surya Dwipa Irsyam; Reza Raihandhany; Muhammad Rifqi Hariri; Rina Ratnasih Irwanto
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.198

Abstract

Araceae is a group of plants that are consistently dominant in humid and wet conditions areas. This family is among the most common plant groups found in the campus Institut Teknologi Bandung (ITB) at Jatinangor, which are still covered by green areas. However, the diversity of Araceae has not been revealed yet. Therefore, this research was conducted to provide information on the Araceae in ITB Jatinangor Campus. Field exploration was carried out during September - November 2021 at ITB Jatinangor. As many as 15 genera and 22 species of Araceae have been discovered within the campus. Taxonomically, they are grouped into 3 subfamilies, i.e Aroideae (12 genera), Monsteroideae (2 genera), and Pothoideae (1 genus). The largest genera of Araceae found on the campus were Anthurium and Philodendron, both cultivated as ornamental plants. Several local species, such as Alocasia macrorrhizos, Colocasia esculenta, Pistia stratiotes, Typhonium flagelliforme, and T. roxburghii were also found. Three other ornamental species have been escaped from cultivation, namely Caladium bicolor, Dieffenbachia seguine, and Syngonium podophyllum had were also found here. This research was part of the Flora Campus ITB Jatinangor Exploration, a project initiated by Herbarium Bandungense.
INDUCTION OF POLYPLOID BANANA KEPOK THROUGH IN VITRO ADDITION OF FLAME LILY EXTRACT Eti Ernawiati; Lili Chrisnawati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.201

Abstract

Flame lily (Gloriosa superba L.) contains colchicine and gloriosin in almost all parts of the plant, especially in tubers and seeds. Colchicine is often used in biological and breeding studies to induce mutations that result in polyploid plants. This study aimed to examine the effectiveness of flame lily tuber extract as a natural mutagen for the development of superior kepok banana cultivars through the in vitro assembly of polyploid kapok bananas. The study was arranged completely randomly with two factors. Factor 1 consisted of 3 levels, namely fresh extract of breech flower tubers (10 %), pure colchicine solution (0.1%) as a positive control, and without being added (0%) as a negative control. Factor 2 consists of 3 levels, namely Kepok Abu, Kepok Batu and Kepok Kuning. All treatment combinations were repeated 5 times. The data were analyzed by Diversity Test (Sidik Ragam) and if there is a difference, it will be continued with the DMRT test at a level of 5%. The results showed that the administration of colchicine in culture media could induce the emergence of polyploid banana plantlets when viewed from the addition of the size of the number of shoots, shoot length, number of roots, root length, leaf area, epidermal cells, stomata, and decreased stomata index. Meanwhile, 10% breech flower tuber extract was not able to induce the emergence of polyploid plantlets. Kepok Kuning bananas had a better response to mutagens than Kepok Abu and Kepok Batu. From these results, it can be concluded that 0.1% colchicine was able to induce polyploid banana plantlets and 10% breech flower tuber extract was not able to induce polyploid plantlets.
DETECTION OF NITROGENASE PRODUCING BACTERIA FROM THE SOIL OF LIWA BOTANICAL GARDEN Cristina Nugroho Ekowati; Mica Mirani; Kusuma Handayani; Rochmah Agustrina
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 2 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i2.204

Abstract

Liwa Botanical Gardens is an ex-situ conservation area for various types of plants. Each plant produces organic matter that will provide nutrients for the growth of nitrogen-fixing bacteria. This indicates the existence of an environment that supports the growth of nitrogen-fixing bacteria. Nitrogen is one of the nutrients needed by plants for their growth. However, the abundance of nitrogen in the atmosphere cannot be utilized directly by plants but needs to transform into ammonium and nitrate first. This transformation can be done by nitrogen-fixing bacteria through an enzymatic process. This research aims to obtain bacterial isolates that can fix nitrogen. Nitrogen-fixing bacteria were isolated using Nutrient Agar (NA) medium and furthered by nitrogenase activity detection test with semi-solid Nitrogen Free Bromothymol Blue (NFB). Nitrogen-fixing bacteria are characterized by color changes in the medium. The results obtained 22 isolates with 3 isolates detected capable of producing nitrogenase enzymes, namely TBP B3, TB1 B2, and TMA2 B2.

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