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Journal of Microbial Systematics and Biotechnology
Published by Cibinong Science Center
ISSN : -     EISSN : 26854430     DOI : https://doi.org/10.37604/jmsb
Core Subject : Science,
Immunology & microbiology
Journal of Microbial Systematics and Biotechnology (JMSB) is the international journal of microbial diversity and microbial technology which publishes research articles, reviews, and methodologies of microbial-based technology; and taxonomic articles such as monographs, new species, new notes, new records, checklists related to microbial diversity. The official language is English. Every manuscript submitted to JMSB will be published as soon as the editor receives it, and through the peer review process.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi
Articles 6 Documents
 1 
Search results for , issue "Vol 2, No 1 (2020): June 2020" : 6 Documents clear
Stability of Phycocyanin Extracted from Spirulina maxima in different pH from Indoor and Semi-outdoor Cultivation Dwi Susilaningsih; Bimo Saskiaoktavian; Ali Djunaedi; Agus Trianto
Journal of Microbial Systematics and Biotechnology Vol 2, No 1 (2020): June 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i1.33

Abstract

Spirulina maxima is a blue-green microalga that rich in pigment. The pigments in S. maxima grouped into primary pigment (Chlorophyll a) and accessory pigments (carotenoid & phycobiliprotein). Phycocyanin is an accessory pigment that belongs to phycobiliprotein, blue colored, and can be used as natural food coloring and drugs. Phycocyanin has activities as antibacterial, antioxidant, anti-inflammatory, antihyperalgesic, and many more. Because of that, phycocyanin usually used in the pharmaceutical industry. However, phycocyanin is a protein that unstable under lights, high temperature, and pH in the storage. This study aims to obtain information about the effect of pH on the stability of phycocyanin extracted from S. maxima that cultivated in indoor and semi-outdoor. The steps are cultivation, extraction using different solutions to get blue pigment phycocyanin, and stability test. Phycocyanin was dissolved in a buffer solution at pH 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, and 7.0 for 7 days. Color observations based on visualization and concentration measurements are carried out every day to see changes in phycocyanin. Growth in indoor cultivation with 24 hours light is faster than semi-outdoor a that uses only sunlight. Cultivation gets the optical density value 0,6 at day 20 with indoor cultivation and day 34 with semi-outdoor cultivation. Results show that phycocyanin can be extracted using a buffer phosphate solution. The stability of the pigments can be seen from the color changes and relative concentration using a spectrophotometer. Phycocyanin shows stable in the storage of pH 4 until pH 5.5. The highest relative concentration (CR) was shown in pH 5.
Cover JMSB Vol 2, No 1 (2020) Cover and Table of Content JMSB
Journal of Microbial Systematics and Biotechnology Vol 2, No 1 (2020): June 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i1.44

Abstract

Evaluation of anti-Fusarium and auxin production of Trichoderma virens InaCC F1030 isolated from rhizosphere of banana Toga Pangihotan Napitupulu; Indriati Ramadhani; Atit Kanti; I Made Sudiana
Journal of Microbial Systematics and Biotechnology Vol 2, No 1 (2020): June 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i1.39

Abstract

Banana rhizosphere harbors a unique diversity of microbes including fungi that play critical roles in the growth of the plant host as well as might be important for biologically controlling the fungal soil-borne pathogens particularly Fusarium oxysporum f.sp. cubense (Foc), the causing agent of devastating Panama wilt. Among other fungi, we have succeeded to isolate a Trichoderma species from rhizosphere of healthy banana. Molecular identification revealed the isolate as Trichoderma virens InaCC F1030 (being collection of Indonesian Culture Collection or InaCC). Therefore, the aim of this study was to investigate the biological control of our isolate against Foc as well as plant growth promoting ability through its ability to produce auxin (indole-3-acetic acid/IAA). Two approaches were employed to evaluate the antagonism of our isolate against Foc, through direct confrontation test and volatile organic compounds (VOCs) producing. We found that our isolate was considered as antagonistic to the Foc, but not highly antagonistic according to direct confrontation assay. However, it was also revealed that our isolate produces the VOCs that inhibited around 50% of the mycelial growth of the test pathogen after six to seven days of exposure. Our isolate was able to produce the IAA in axenic submerged fermentation condition particularly in the presence of the precursor L-tryptophan. IAA production ability as well as the mycelial biomass of fungus were increased approximately 17% and 120% respectively as the effect of supplementation of 0.1% of L-tryptophan. These in vitro bioassays lead us to conclude that somehow our isolate T. virens InaCC F1030 has potency to be utilized as biocontrol and biofertilizer agent.
Microbial isolation from gastrointestinal tract of catfish (Clarias gariepinus) Nurul Aini; Muhammad Bachruddin; Izdihar Tsana
Journal of Microbial Systematics and Biotechnology Vol 2, No 1 (2020): June 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i1.38

Abstract

The purpose of this research was to describe the characteristics and number of microbes that grow in the gastrointestinal tract of catfish and to determine the potential of microbes as probiotics. The type of this research was observational conducted on 30 September-15October 2018 at the Microbiology Laboratory, Faculty of Science and Technology, Universitas Airlangga, Surabaya. Microbial isolation using Nutrient Agar (NA), Mann de Roosa Shrape agar (MRSA) and potato dextrose agar (PDA) media. The fish that used is catfish that are bred in ponds at Desa Tlasih, Kecamatan Tulangan, Kabupaten Sidoarjo, East Java. The identification includes microscopic and macroscopic characteristics. Based on the research that has been done, it can be concluded that there are 8 kinds of bacterial colonies in NA media, on PDA media there are 8 kinds of fungi, and on MRSA media there are 2 bacterial colonies and each has different macroscopic and microscopic characteristics. The total number of bacteria growing in NA and MRSA media respectively were 8.7 x 104 CFU/g and 1.2 x 105 CFU/g. It is suspected that there are potential bacteria as beneficial probiotics for catfish which still need further research.
Challenge of bacteriophage application to improve food safety and its administration into the human gut: an article review Qori Emilia
Journal of Microbial Systematics and Biotechnology Vol 2, No 1 (2020): June 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i1.36

Abstract

Ensuring microbial food safety has always been a challenge at every stages along the food chain. Meanwhile, healthier community lifestyle demands natural antimicrobial agents to alleviate the increasing use of chemical preservatives to address microbial contamination. Antimicrobial resistance issue has also elevated the effort to search for an alternative way to antibiotics. Bacteriophage (phage) is currently being assessed for its potency as a biocontrol agent to enhance food safety and as a tool for therapeutic purposes. Prior to phage application, safety assessment must be conducted in which includes several considerations: from the discovery, toxicological aspects to the impact of phage ingestion on the gut microbiota. The gut microbiota which consist of variety of microorganisms inside the human gastrointestinal tract, cohabitate to each other. Phage is naturally present as one of microorganisms in the human gut and dynamically interacted with other microbial communities. Phage application to foods and food-contact surfaces may leave a residue and cause the phages to be ingested, which in result may alter the gut microbiota composition. Many findings have examined the relationship between gut microbiota and human health, and so is the factors affecting their modulation. This review aimed to discuss several points of view from published research papers related to the challenge of phage to improve food safety and its administration into the human gut.
A molecular phylogenetic study of Clarohilum henningsii (Mycosphaerellaceae, Fungi) on cassava from Indonesia based on the ITS rDNA sequence Iman Hidayat; Aerma Hastuty; Indriati Ramadhani
Journal of Microbial Systematics and Biotechnology Vol 2, No 1 (2020): June 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i1.43

Abstract

Leaf spot is a common disease of cassava in Indonesia and other tropical countries. The present study aims to determine two isolates isolated from leaf spot of cassava in Indonesia using a molecular phylogenetic analysis based on internal transcribed spacer (ITS) rDNA sequence and morphological examination. The results showed that the two isolates belong to Clarohilum henningsii. This study revealed the phylogenetic position of C. henningsii from Indonesia, where in the previous studies, it has been reported as Cercospora cassavae, C. manihotis, C. henningsii, or Passalora henningsii based on morphological examinations.

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