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Debora Christin Purbani
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Journal of Microbial Systematics and Biotechnology
Published by Cibinong Science Center
ISSN : -     EISSN : 26854430     DOI : https://doi.org/10.37604/jmsb
Core Subject : Science,
Immunology & microbiology
Journal of Microbial Systematics and Biotechnology (JMSB) is the international journal of microbial diversity and microbial technology which publishes research articles, reviews, and methodologies of microbial-based technology; and taxonomic articles such as monographs, new species, new notes, new records, checklists related to microbial diversity. The official language is English. Every manuscript submitted to JMSB will be published as soon as the editor receives it, and through the peer review process.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi
Articles 6 Documents
 1 
Search results for , issue "Vol 2, No 2 (2020): December 2020" : 6 Documents clear
The influence of biocarrier of Aspergillus niger and Trichoderma harzianum toward vegetative growth of sorghum in the field experiment Arwan Sugiharto; Toga Pangihotan Napitupulu; I Made Sudiana
Journal of Microbial Systematics and Biotechnology Vol 2, No 2 (2020): December 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i2.60

Abstract

Compared to other cereal crops, sorghum has a higher drought tolerance trait. However, efforts are needed to increase the productivity of sorghum, particularly in drought marginal land. One strategy to be implemented is the utilization of soil microorganisms formulated with biocarrier. Therefore, the aim of this study was to evaluate the influence of the fungal strain Aspergillus niger and Trichoderma harzianum formulated with compost and zeolite as biocarrier towards vegetative growth of sorghum. The field experiment was designed as a randomized block designed, factorial pattern with 4 replications. The first factor was selecting biocarrier, namely zeolite, compost, and a mixture of zeolite: compost (1:1). The second factor was the fungal inoculants, A. niger, and T. harzianum. The observed parameter was the growth profile of sorghum during vegetative growth, including stalk diameter and height. The results showed that the type of biocarrier, as well as the fungal strains did influence the growth of sorghum. The highest stalk diameter and height of sorghum were obtained after application of A. niger formulated with a mixture of zeolite: compost (1:1), with 17% and 41.2% higher than control, respectively. This condition shows that a mixture of zeolite and compost is seemingly able to create better micro-ecological conditions for fungal microbes to function effectively. Therefore, our findings suggested the addition of zeolite to compost for the application of biocarrier in the field experiment.
Extraction, characterization, and biological toxicity of β-glucans from Saccharomyces cerevisiae isolated from ragi Indriati Ramadhani; Diva Larissa; Yeni Yuliani; Mellova Amir; Kusmiati Kusmiati
Journal of Microbial Systematics and Biotechnology Vol 2, No 2 (2020): December 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i2.62

Abstract

β-glucan is a homopolysaccharide with biological activities that are beneficial to health as an immunostimulant, anti-inflammatory, anti-diabetic, anti-cholesterol, and many more. β-glucan extraction results from yeast require characterization related to this bioactive quality, such as β-glucan weight, monomer analysis, functional groups, and cytotoxicity assay. Four Saccharomyces cerevisiae isolates were isolated from three local ragi samples, namely the SC-1, SC-2, SC-3, and SAF from instant ragi. This study aimed to obtain the best candidate of S. cerevisiae isolates to produce high β-glucan levels and low protein levels and to test the potential for cytotoxicity. The four isolates were rejuvenated on potato dextrose agar (PDA), then inoculated into the liquid glucose yeast peptone (GYP) fermentation medium for six days. Saccharomyces cerevisiae cells were extracted by neutralizing acid-base, dried and weighed as a crude β-glucan (mg per 300 mL). The highest yield was SC-2 (818 mg), followed by SC-3 (726 mg), SAF (597 mg), and SC-1 (433 mg). The presence of –OH (alcohol), -C-C-C- (alkane), and –R-O-R- (ether) groups were showed using FTIR characterization. Glucose equivalent β-glucan levels and protein levels were determined using a UV-Vis spectrophotometer. The results showed that β-glucan SC-1 gave the best results with glucose equivalent β-glucan levels of 4,865% and protein levels of 3,804%. The crude β-glucan toxicity test using the brine shrimp lethality test (BSLT) method shows that the β-glucan of the SAF strain has LC50 cytotoxicity of 114.8 ppm followed by β-glucan cytotoxicity from local ragi LC50 was SC-2 (323.5 ppm), SC-1 (331.1 ppm), and SC-3 (354.8 ppm). Therefore, based on the results, SC-1 isolate obtained the highest β-glucan crude and the lowest protein content was SC-2. The β-glucan of SAF extract had the highest toxicity properties based on the IC50 value.
Development of a dimer-based screening system for dimerization inhibitor of HIV-1 protease I Dewa Agung Panji Dwipayana; Yana Maolana Syah; Reza Aditama; Feraliana Feraliana; Azzania Fibriani
Journal of Microbial Systematics and Biotechnology Vol 2, No 2 (2020): December 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i2.42

Abstract

An in vitro dimer-based screening system (DBSS) for selecting new HIV-1 protease dimerization inhibitor candidates from natural compounds had been established. This system utilizes a fusion between HIV-1 protease and dimer binding domain of AraC protein (proteaseHIV1-AraCDBD) where fluorescence signal will be emitted in the presence of HIV-1 protease inhibitor. However, this screening system had not been evaluated. Therefore, this study was aimed to evaluate it in recombinant Escherichia coli culture. The expression of proteaseHIV1-AraCDBD fusion gene was observed for 18 hours. Its crude lysate isolation was done once every 3 hours and analyzed using SDS PAGE. To test the DBSS, darunavir was used as positive control, and Nigella sativa extract (JH3) was used as the test compound. The results of SDS PAGE analysis on crude lysates presented a ~24.2 kDa band, which was the predicted size of the proteaseHIV1-AraCDBD fusion protein based on its amino acid sequence. The growth curve and protein expression profiles revealed that the 15 hours was the optimum culture age to be used in the screening system. Darunavir testing in DBSS showed an increase in fluorescence signal compared to the negative control. The same increase in fluorescence signal was also obtained from the JH3 compound test. In conclusion, DBSS could be used as an assay to screen for new HIV-1 protease inhibitors, and the JH3 compound demonstrated the ability to inhibit HIV-1 protease dimerization.
Cover JMSB Vol 2, No 2 (2020) Cover and Table of Content JMSB
Journal of Microbial Systematics and Biotechnology Vol 2, No 2 (2020): December 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i2.65

Abstract

Culturable bacterial abundance in Volvariella volvacea cultivation medium and characterization of its bacteria Masrukhin Masrukhin; Iwan Saskiawan
Journal of Microbial Systematics and Biotechnology Vol 2, No 2 (2020): December 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i2.52

Abstract

Straw mushroom (Volvariella volvacea) is one of the popular edible fungi in Indonesia. Previous researches showed the correlation among the type of substrate, substrate quality, and its composting process to the microbial community, yield, and biological efficiency. The aim of the research is to analyze the culturable bacteria abundance in straw mushroom cultivation medium, characterize the bacteria in several stages of mushroom cultivation and investigate the interaction between V. volvacea with its resident bacteria. Samples were taken from mushroom farmers in Subang and Karawang regencies, Indonesia. The materials for cultivation medium are the mixture of cotton and paddy straw and the pasteurization was performed at 65-70°C for 7 hours. The result shows the abundance of the bacteria in most of the cultivation stages is relatively similar i.e. 108 CFU/g, except in 15 days after inoculation (DAI), the bacterial abundance is lower i.e.6.24 x 107 CFU/g. Twenty-five isolates were obtained and Gram-positive bacteria is the dominant bacteria found in the cultivation medium, especially rod-shaped Gram-positive bacteria. According to co-culture assay there are nine isolates that decrease the growth rate and clearly inhibit mycelial growth. The other 10 isolates have lower inhibitory activity, and 6 isolates have no inhibitory activity to the mycelial growth. C38 isolates have the highest mycelial growth inhibition. It belongs to rod-shaped Gram positive group of bacteria which isolated from the early stage of V. volvacea cultivation medium (5 DAI).
Effect of ethanol extract from Karuk leaf (Piper sarmentosum Roxb.) on the growth of Malassezia furfur in vitro Khusnul Khusnul; Asti Kusmayanti; Lia Aulia Rahman; Nuniek I Ratnaningtyas
Journal of Microbial Systematics and Biotechnology Vol 2, No 2 (2020): December 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i2.59

Abstract

In Indonesia, there are numerous therapeutic plants found. Some of the plants used in herbal medicine are Karuk leaf (Piper sarmentosum Roxb.) belong to the Piperaceae family. Karuk leaf has chemical contents such as saponins, polyphenols, flavonoids, and essential oils and many tests are carried out on several bacteria, but testing of fungi is rarely studied. This study aims to determine the ethanol extract activities from karuk leaf in inhibiting the growth of the Malassezia furfur fungus and to determine its minimum inhibition by using the Kirby-Bauer method. The study was conducted by an experimental method of the M. furfur fungus using the Kirby-Bauer method. The ethanol extract from karuk leaf was made in various concentrations and tested on 0.5 McFarland fungus by diffusion on Sabouraud Dextrose Agar (SDA). The results of this analysis showed that the ethanol extract of Karuk leaf could inhibit the M. furfur fungus at a concentration of 30% by 5.3 mm, 40% by 6.6 mm, 50% by 7.6 mm, 60% by 11.3 mm, 70% by 12.5 mm, 80% by 15.6 mm, 90% by 17.4 mm, and 100% by 19.5 mm. Based on the results of the study and the data analysis, it can be concluded that several concentrations of ethanol extract of Karuk leaf affect the growth of M. furfur in vitro.

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