cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Debora Christin Purbani
Contact Email
editor@e-jmsb.id
Phone
+6221-8761356
Journal Mail Official
editor@e-jmsb.id
Editorial Address
Jln. Raya Jakarta-Bogor KM. 46 Cibinong 16911, West Java, Indonesia
Location
Kota bogor,
Jawa barat
INDONESIA
Journal of Microbial Systematics and Biotechnology
Published by Cibinong Science Center
ISSN : -     EISSN : 26854430     DOI : https://doi.org/10.37604/jmsb
Core Subject : Science,
Immunology & microbiology
Journal of Microbial Systematics and Biotechnology (JMSB) is the international journal of microbial diversity and microbial technology which publishes research articles, reviews, and methodologies of microbial-based technology; and taxonomic articles such as monographs, new species, new notes, new records, checklists related to microbial diversity. The official language is English. Every manuscript submitted to JMSB will be published as soon as the editor receives it, and through the peer review process.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi
Articles 6 Documents
 1 
Search results for , issue "Vol 3, No 1 (2021): August 2021" : 6 Documents clear
Biosynthesis and profiling of single cell carotenoids of Phaf ia rhodozyma in waste-based cultivation media Arif Nurkanto; Rini Handayani; Ismu Purnaningsih; Mia Kusmiati; Ninu Setianingrum; Mulyadi Mulyadi; Endang Kusdiyanti; Achmad Dinoto
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.61

Abstract

In order to investigate the feasibility of low-cost media for producing well- characterized single-cell carotenoid, the study on biosynthesis and profiling carotenoid in the yeast of Phaf ia rhodozyma was carried out. We have successfully identified the profiles of single-cell carotenoids of P. rhodozyma, which was cultivated in glucose-based medium (MG), molasses-based medium (MM), and coconut water-based medium (MC). Cells were separately cultured in those media under aerobic batch culture system to obtain the carotenoid profiles based on high performance liquid chromatography (HPLC) analysis. The results showed that medium composition strongly affects the profiles of P. rhodozyma carotenoids represented by ratio of astaxanthin and beta-carotene (ratio A/B). Astaxanthin was highly synthesized in cells cultivated in MG with ratio A/B as much of 20:1. On the other hand, MM and MC produced a lower ratio A/B than MG as much of 0.4:1 and 0.2:1, respectively. In addition, carotenoids profiles were detected more diverse when this yeast species was cultivated in two waste-based media. This study provided a basic physiological knowledge of P. rhodozyma cells for carotenoid biosynthesis using potential low cost cultivation m
Cover and Table of Content JMSB Vol 3, No 1 (2021) Cover and Table of Content JMSB
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.82

Abstract

Analysis of the SARS-CoV-2 envelope (E), nucleocapsid (N), and non-structural protein12 (nsp12) genes from COVID-19 patients in West Java Azzania Fibriani; Irin Annisa Evitayani; Gusti Ayu Prani Pradani; Rebecca Stephanie; Ema Rahmawati; Ryan Bayusantika Ristandi; Cut Nur Cinthia Alamanda; Rifky Waluyajati Rachman; Rini Robiani; Isak Solihin
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.66

Abstract

According to World Health Organization, as of January 2021, Indonesia is the only Southeast Asian country in which COVID-19 is still occurring in community transmission. West Java is one of the provinces holding the highest positive cases number. With the envelope (E), nucleocapsid (N), and non-structural protein 12 (nsp12) being the target genes of SARS-CoV-2 diagnostic kits and several antiviral drugs, the study of genetic variations has become relevant and greatly important. Out of 267 oro-nasopharyngeal swab specimens that were previously confirmed positive for COVID-19 in qPCR diagnostic test in Laboratorium Kesehatan Provinsi Jawa Barat, ten samples with acceptable qualities were selected and three samples were sequenced using Sanger sequencing. Nonsynonymous mutations were observed in the envelope gene (L21F) and in the nucleocapsid genes (R203K, G204R, A211S, and S193I). Phylogenetic analysis showed that samples were clustered with other sequences carrying identical mutations, but clustered non-discriminatively with all sequences when carrying no mutation. No pattern in geographical areas and clades, except for R203K-G204R for being a marker for the GR clade. Protein structure analysis showed that mutations observed did not change the hydrophobicity and the secondary structure of the nucleocapsid, while stability change (ΔΔG) showed that all mutations, aside from the R203K-G204R, have neutral effect on the protein stability. Therefore, it can be concluded that mutations observed in this experiment did not impart preference to disperse in certain geographical areas or cause any significant structural change in the protein.
Mycorrhiza stimulates Rhizobium infection in Paraserianthes falcataria (L.) I.C. Nielson under Hg contamination Irma Latifah; Idris Idris; Toga Pangihotan Napitupulu; Azra Zahra Nadirah Ikhwani; Gunawan Ruhiyat; I Nyoman Sumerta; Atit Kanti; Fitri Yola Amandita; I Made Sudiana
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.70

Abstract

Symbiosis of and AMF increase soybean production, but the information on the association between these microbes in Paraserianthes falcataria (L.) I.C. Nielson or ‘sengon’ under Hg contamination is limited. We observed P. falcataria inoculated with arbuscular mycorrhiza fungi (Glomus sp.) stimulate nodule formation under Hg contamination. The study was set up in a pot experiment in the growth chamber a the Hg concentration was adjusted to 50 ppm in soil medium. Inoculation of AMF stimulates nodule formation and increases P. falcataria tolerance to Hg. We isolated several bacteria from the rhizosphere belonging to Rhizobium group and others. Understanding the ecology of soil bacteria is important for Hg bioremediation using P. falcataria.
Characterization and application of nitrogen-fixing and indole-3-acetic acid producing bacteria A13 in Oil Palm (Elaeisguineensis Jacq.) seedling Ismi Isti'anah; Nisa Rachmania Mubarik; Aris Tjahjoleksono
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.77

Abstract

Oil palm plantations have a good prospect in Indonesia. One of the efforts to improve the productivity of oil palm plantation is the application of bacteria as biological fertilizer. The research was conducted to characterize and apply the nitrogen-fixing and indole-3-acetic acid producing bacteria in oil palm seedlings. The bacteria was isolated from soil samples which taken from Taman Nasional Bukit Dua Belas (TNBD) Jambi. Nitrogen free bromthymol blue (NFB) is used as media for nitrogen-fixing bacterial isolation. Selected isolate named A13 had an ability to form white pellicle on the surface of the semisolid medium, increased the pH, and changed the color of medium from green to blue Isolate A13 was identified as Gram-negative bacteria and had a rods shape. Analysis of 16S rRNA gene sequence showed that isolate A13 had a similarity with Pseudochrobactrum assacharolyticum. Hypersensitivity assay on tobacco leaves showed that isolate A13 was not a pathogen. During 48 hours of incubation, isolate A13 produced a maximum of IAA at the 24th hour of incubation. Isolate A13 produced 0.675 ppm of ethylene/hour in Acetylene Reduction Assay and 69,839 ppm of IAA in HPLC methods. This was the first report on nitrogen fixation and IAA production by Pseudochrobactrum assacharolyticum and its application in the soil of oil palm seedlings. Application of isolate A13 in oil palm seedling increased significantly the number of lateral roots, stem diameter, and height of plants
Effect of Clove essential oil (Syzygium aromaticum) against the growth of dandruff scalps-causing fungal pathogen using Kirby- Bauer method in vitro Khusnul Khusnul; Puput Putuh Anisa; Dewi Peti Virgianti
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.78

Abstract

One of the strongest contributing factors in dandruff scalps is the presence of fungal pathogen or called as Malassezia. Several studies have found a connection between Malassezia and scalp health, one of which is caused by pathogenic fungal infections including Pityrosporum ovale, Microsporum gypseum, and Candida albicans. Clove flowers are known to contain eugenol and its derivative compounds which have antimicrobial, antifungal, antiseptic, and local anesthetic activity. This research aims to determine the effect of clove flower essential oil (Syzygium aromaticum) against some dandruff-causing fungi. The research method was to test the inhibitory power of clove flower essential oil using the diffusion method (Kirby Bauer technique). Samples of pathogenic fungi were cultured on SDA media (Saboroud Dextrose Agar) and given discs that had been given several concentrations of clove flower essential oil. The concentrations used were 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, positive control using 2% ketoconazole and negative control using virgin coconut oil (VCO). The results showed a significant effect between the ability of essential oils to inhibit the growth of some fungi that cause dandruff. The treatment of clove flower essential oil against M. gypseum at the concentration of 10% has an inhibitory ability of 33,05 mm and at the concentration of 100% amounted to 63.95 mm. Meanwhile, the inhibition percentage in P. ovale with a concentration of 10% and 100% of extract were 14,75 mm and 46,15 mm, respectively. The inhibition rate in C. albicans with the concentration of 10% extract and 100% of extract was 13,5 mm and 39,35 mm. The research shows that clove flower essential oil inhibits the growth of several pathogenic fungal in vitro.

Page 1 of 1 | Total Record : 6

 1 

Filter by Year

2021 2021


Reset
Filter By Issues
All Issue Vol 3, No 1 (2021): August 2021 Vol 2, No 2 (2020): December 2020 Vol 2, No 1 (2020): June 2020 Vol 1, No 2 (2019): December 2019 Vol 1, No 1 (2019): June 2019