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Jurnal Penelitian Tanaman Industri (Littri)
Published by Pusat Penelitian dan Pengembangan Perkebunan
ISSN : 08538212     EISSN : 25286870     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal Penelitian Tanaman Industri (JLITTRI) aims to publish primary research articles of current research topics, not simultaneously submitted to nor previously published in other scientific or technical ojournals. General review articles will not be accepted. The journal maintains strict standards of content, presentation,and reviewing. SCOPE The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of agricultural science in Indonesia including: Estate crops; Soil science; Climate science; Agronomy; Plant breeding; Biotechnology; Genetic resources; Plant pathology; Plant physiology; Entomology; Farming system; Postharvest technology; Socio-economic agriculture; Environment; Agricultural extension. The journal publishes Indonesian or English articles. Since the year of 2017, the jurnal is published twice a year in (June and December).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 6 Documents
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Search results for , issue "Vol 18, No 4 (2012): Desember 2012" : 6 Documents clear
ANALISIS EFISIENSI USAHA TANI TEBU DI JAWA TIMUR SRI HERY SUSILOWATI; NETTI TINAPRILLA
Jurnal Penelitian Tanaman Industri Vol 18, No 4 (2012): Desember 2012
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v18n4.2012.162-172

Abstract

ABSTRAKUpaya pengembangan usaha tani tebu masih terkendala bukanhanya oleh ketersediaan lahan namun juga oleh aspek teknis budidayausaha tani (penggunaan bibit unggul, pemupukan, aspek kelembagaan,dan sebagainya). Selain melalui fasilitasi perluasan lahan, strategipengembangan  tebu  harus  disertai  dengan  upaya  peningkatanproduktivitas, yaitu melalui peningkatan efisiensi usaha tani tebu, ataudengan kata lain bagaimana meningkatkan output maksimum melaluipengelolaan sumberdaya serta teknologi yang ada. Tujuan penelitianadalah untuk menganalisis efisiensi usaha tani tebu dan menentukanfaktor-faktor yang mempengaruhi inefisiensi usaha tani tebu. Data yangdigunakan adalah data survei PATANAS (Panel Petani Nasional) olehPusat Sosial Ekonomi dan Kebijakan Pertanian di Kabupaten Malangdan Lumajang, Jawa Timur tahun 2009. Jumlah contoh sebanyak 132rumah tangga yang dipilih secara acak. Analisis menggunakan stochasticfrontier production function approach dengan fungsi produksi StochasticFrontier Cobb Douglas yang diolah menggunakan program Frontier 4.1.Hasil penelitian menunjukkan nilai indeks efisiensi teknis dikategorikanbelum efisien dengan rata-rata efisiensi sebesar 0,672. Variabel akseslahan oleh petani merupakan faktor yang paling penting dan responsifdalam mempengaruhi produksi tebu. Kebijakan pengembangan usahatani tebu untuk meningkatkan efisiensi usaha tani adalah melaluipeningkatan akses lahan, kualitas bibit yang dipakai, dan ketersediaansarana produksi.Kata kunci : efisiensi teknis, usaha tani tebu, stochastic frontierproduction functionABSTRACTImproving sugar cane farming is still constrained by not only landavailability but also technical aspects such as quality of seed, fertilization,institution, etc., so that development strategy to improve sugar canefarming should be conducted by facilitating extensification andincreasing productivity and technical efficiency, or in other wordincreasing maximum output through resource management andtechnology. The aim of this study was to analyze technical efficieny ofsugar cane farming and to identify determinant factors influencing theefficieny of sugar cane farming. This study used PATANAS data surveywhich was conducted by Indonesian Center for Agriculture SocioEconomic and Policy Study (ICASEPS) in Malang and LumajangRegency, East Java Province in the years of 2009. The 132 samplies ofsugarcane household were chosen randomly in the year 2009. Data wereanalysed using stochastic frontier production function approach withStochastic Frontier Cobb Douglas using frontier 4.1. programme. Theresult of this study showed that sugar cane farming in East Java wastechnically not efficient with the index value of 0.672. Among variablesthat significantly influenced sugarcane production, land access byfarmers was an essential factor to improve production. Policy implicationfor developing sugar cane farming to improve technical efficiency is byincreasing land access, quality of seed, and production factor availability.Key words : technical efficiency, sugarcane farming, stochasticfrontier production function
KEEFEKTIFAN FORMULA MINYAK CENGKEH DAN SERAI WANGI TERHADAP Fusarium oxysporum f.sp. vanillae PENYEBAB BUSUK BATANG VANILI MESAK TOMBE; DARMAWAN PANGERAN; TRI SAPTARI HARYANI
Jurnal Penelitian Tanaman Industri Vol 18, No 4 (2012): Desember 2012
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v18n4.2012.143-150

Abstract

ABSTRAKBusuk batang vanili (BBV) yang disebabkan oleh Fusariumoxysporum f.sp. vanillae (Fov) merupakan patogen penting pada vanili danmenyebabkan kerugian besar setiap tahunnya di Indonesia. Jumlahkerugian diperkirakan sebesar 3.000 ton atau sekitar US$ 16 juta.Penelitian dilaksanakan di laboratorium dan rumah kaca Kelti Hama danPenyakit, Balai Penelitian Tanaman Rempah dan Obat, Bogor pada bulanFebruari 2010 sampai Agustus 2010. Tujuan penelitian adalah untukmengetahui pengaruh formula dengan bahan aktif minyak cengkeh danserai wangi terhadap penyakit BBV dan dampaknya terhadap kehidupanmikroorganisme tanah. Dalam pelaksanaannya, kegiatan penelitian dilaku-kan dalam 3 tahap yaitu : (1) uji formula in vitro, (2) uji formula in vivo,dan (3) uji dampak formula terhadap mikroorganisme tanah. Percobaantahap 1, 2, dan 3 masing-masing terdiri atas 10, 6, dan 6 perlakuan,dan.masing-masing disusun menggunakan rancangan acak lengkap dengan3 ulangan. Materi yang dikaji dalam penelitian ini adalah (1) formuladengan bahan aktif minyak cengkeh (formula standar), (2) formula denganbahan aktif minyak cengkeh dan serai wangi (formula baru), dan (3)fungisida sintetis berbahan aktif mankozeb sebagai pembanding. Hasil ujiin vitro menunjukkan bahwa fungisida nabati CS (minyak cengkeh + seraiwangi) pada ke 3 tingkatan konsentrasi (terutama konsentrasi 400 ppm)memperlihatkan efektivitas yang tinggi dalam menghambat pertumbuhanmiselium dan produksi spora patogen Fov.F117 dibandingkan perlakuanlain dan kontrol. Hasil uji in vivo menunjukkan hal yang sama bahwafungisida nabati CS memperlihatkan efektivitas yang tinggi dalammenekan intensitas serangan patogen BBV, terutama pada dosis aplikasi 5ml/l. Dampak perlakuan terhadap kehidupan mikroorganisme tanah (fungidan bakteri) memperlihatkan bahwa penggunaan fungisida sintetismankozeb menghambat kehidupan mikroorganisme tanah 90-100% jikadibandingkan dengan kontrol. Populasi mikroorganisme tanah pada semuaperlakuan fungisida nabati lebih tinggi jika dibandingkan dengan fungisidamankozeb. Populasi mikroorganisme pada aplikasi fungisida nabati padadosis 5 ml/l tidak berbeda nyata dengan kontrol. Penelitian inimenunjukkan bahwa penggunaan fungisida nabati minyak cengkehterutama jika dikombinasikan dengan minyak serai wangi mempunyaiprospek untuk digunakan dalam pengendalian penyakit BBV denganinterval aplikasi 3-4 minggu sekali.Kata kunci : Vanilla planifolia, Fusarium oxysporum f.sp, vanillae, busukbatang vanili, minyak cengkeh, minyak serai wangiABSTRACTVanilla stem rot (VSR) disease caused by Fusarium oxysporum f.sp.vanillae (Fov) is an important disease on vanilla and causes severe lossannually in Indonesia. The total annual loss in production due to the VSRwas estimated to be 3,000 ton or about US$ 16 million. This research wascarried out in the laboratory and green house of Pest and Disease ofResearch Institute for Spices and Medicinal Crops, Bogor from February2010 until August 2010. The objective of this research was to study theeffect of formula using active compounds of clove and citronella oils onthe VSR disease and its impact on the survival of soil microorganisms.The research activities were conducted in three stages, namely (1) in vitroformula test; (2) in vivo formula test, and (3) impact test of formula on thesoil microorganisms. Materials studied in this research were (1) formulawith active compound of clove oil (standard), (2) formula with activecompound of clove and citronella oils (new formula), and (3) syntheticfungicide with mancozeb active ingredient as comparison. Every phase ofthis 3 stage experiment was arranged using completely randomized designwith three replicates. First, second, and third phases of the experimentconsisted of 10, 6, and 6 treatments, respectively. In vitro test resultscarried out in the laboratory indicated that botanical formula CS (clove andcitronella oils of the three concentration levels, especially on the 400 ppm)showed high effectiveness on inhibiting mycelium growth and sporeproduction of Fov.F117 pathogen compared to other treatments andcontrol. In vivo test in the green house indicated the same result thatbotanical fungicide CS (clove dan citronella oils) showed higheffectiveness in suppressing VSR disease infection intensity, especially on5 ml/l dosage. Application of mancozeb synthetic fungicide inhibited 90 –100% soil microorganism livelihood compared to control. However, thesoil microorganism population on all botanical fungicides was higher thanon mancozeb fungicide. The microorganism population on the botanicalfungicide of 5 ml/l dosage was not significantly different from the control(natural soil with no treatment). This research indicates that aplication ofbotanical fungicide (combination between clove and citronella oils) with3 - 4 weeks interval is prospectous to control VSR disease.Key words : Vanilla planifolia, Fusarium oxysporum, f.sp. vanillae,vanilla stem rot, clove oil, citronella oil
KORELASI ANTARA AGRESIVITAS INOKULUM SPORANGIA DENGAN TOKSISITAS FILTRAT Phytophthora capsici ASAL TANAMAN LADA (Piper nigrum L.) CHAERANI CHAERANI; DYAH MANOHARA
Jurnal Penelitian Tanaman Industri Vol 18, No 4 (2012): Desember 2012
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v18n4.2012.173-182

Abstract

ABSTRAKPenggunaan varietas lada tahan penyakit paling praktis dan efektifuntuk menekan serangan Phytophthora capsici, penyebab penyakit busukpangkal batang (BPB) lada, tetapi varietas lada tersebut belum tersedia.Seleksi dini ketahanan lada dapat dilakukan di laboratorium menggunakaninokulum sporangia atau filtrat biakan P. capsici. Tujuan penelitian iniialah membandingkan agresivitas inokulum sporangia dengan toksisitasfiltrat biakan (FB) P. capsici. Penelitian dilakukan dari bulan Juni sampaiSeptember 2009 di laboratorium Biokimia BB Biogen dan Hama danPenyakit Balittro. Penelitian menggunakan 50 isolat P. capsici dariberbagai daerah pertanaman lada yang diisolasi tahun 1982 sampai 2009.Daun-daun lada yang diambil dari varietas Natar-1 (agak tahan terhadap P.capsici) dan Petaling-1 (rentan terhadap P. capsici) diinokulasi denganpotongan agar mengandung sporangia atau 20 µl FB P. capsici. Percobaandilakukan secara faktorial (2 varietas × 50 isolat) dengan rancangan acakkelompok dan tiga ulangan. Derajat agresivitas isolat dan toksisitas FBdiukur berdasarkan luas bercak nekrotik. Hasil penelitian menunjukkanbahwa agresivitas inokulum sporangia dan toksisitas FB bervariasi antarisolat P. capsici. Bercak nekrotik yang disebabkan oleh inokulumsporangia lebih luas (0,0–2.535,2 mm 2 ) dibandingkan dengan FB (0,7–233,0 mm 2 ). Derajat agresivitas isolat dan toksisitas FB sangat dipengaruhioleh asal isolat (P<0,0001), tetapi tidak dipengaruhi oleh varietas daninteraksi isolat×varietas (P>0,05). Derajat ketahanan pada varietas tahanNatar-1 diduga tidak berbasis genetik dan tidak berbeda nyata dari varietasrentan Petaling-1 sehingga kedua metode seleksi ketahanan tidak dapatdibedakan keefektifannya. Luas bercak nekrotik yang diinduksi oleh FBtidak berkorelasi nyata dengan yang ditimbulkan oleh inokulum sporangia(R 2 =0,002; P>0,05), sehingga secara umum FB P. capsici tidak dapatdigunakan sebagai standar pengujian ketahanan lada. Oleh karena itumasih perlu dikembangkan metode inokulasi yang konsisten untuk seleksidini ketahanan lada.Kata kunci: lada, penyakit busuk pangkal batang, Phytophthora capsici,agresivitas, filtrat biakan, seleksi diniABSTRACTResistant varieties are the most practical and effective means tocontrol Phytophthora capsici, the pathogen of foot rot disease of blackpepper. However, no resistant cultivars are available. Early selection ofblack pepper resistance can be performed in laboratory using P. capsiciinocula or culture filtrate. The objective of this study was to compare P.capsici isolate aggressiveness with culture filtrate (CF) toxicity. The studywas conducted from June until September 2009 at the BiochemistryLaboratory of the Indonesian Center for Agriculutral Biotechnology andGenetic Resources Research and Development and the Plant Pest andDisease Laboratory of the Indonesian Research Institute of Spice andMedicinal Crops. The study used 50 P. capsici isolates collected fromvarious black pepper plantations during 1982 until 2009. Detached leavesof two black pepper cultivars, i.e. moderately resistant cv. Natar-1 andsusceptible cv. Petaling-1, were inoculated with agar blocks containingsporangia or 20 µl CF of P. capsici. The experiments were designed asfactorial experiments (2 cultivars × 50 isolates) under a randomizedcompletely block design. Isolate aggressiveness and CF toxicity weremeasured based on the necrotic area of the inoculated leaves. The resultsfrom the two inoculation methods showed varying levels of aggressivenessand CF toxicity among isolates. Necrotic lesions incited by sporangialinoculum were more extensive (0.0-2,535.2 mm 2 ) than those induced byCF (0.7-233.0 mm 2 ). Degree of isolate aggressiveness and CF toxicitywere significantly affected by origins of isolate (P<0.0001), but not bycultivar and isolate×cultivar interaction (P>0.05). Resistance degree in themoderately resistant cv. Natar-1 was presumably not genetically based andwas not different to that in the susceptible cv. Petaling-1, and hence bothselecting agents were unable to discriminate resistance level between thetwo cultivars. Necrotic sizes induced by CF did not well correlate withthose incited by sporangial inocula (R 2 =0.002; P>0.05), indicating that CFis generally not suitable to be used as early selection agent of resistantplants. Therefore, further study is justified to find more reliable inoculationmethod for early detection of resistant black pepper.Key words: black pepper, foot rot disease, Phytophthora capsici,aggressiveness, culture filtrate, early selection in laboratory
PATOGENISITAS NEMATODA Heterorhabditis sp. TERHADAP KUMBANG DAUN KELAPA Brontispa longissima GESTRO WIRATNO WIRATNO; ROHIMATUN ROHIMATUN
Jurnal Penelitian Tanaman Industri Vol 18, No 4 (2012): Desember 2012
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v18n4.2012.137-142

Abstract

ABSTRAKSuatu bioassay telah dilakukan di Balai Penelitian TanamanRempah dan Obat Bogor, dari bulan November 2010 sampai dengan Maret2011, yang bertujuan untuk mengetahui potensi Heterorhabditis sp. dalammengendalikan Brontispa longissima. Nematoda dipanen dengan carameletakkan larva Tenebrio molitor mati terinfeksi nematoda patogenserangga (NPS) di atas kertas saring yang diletakkan di dalam cawan petriberisi 30 ml air. Juvenil infektif (JI) yang ada di dalam tubuh larva akankeluar dan hidup di dalam air. Suspensi JI kemudian dilarutkan 1.000 kalilalu populasinya dihitung di bawah mikroskop binokuler dan diulang 3kali. Populasi JI diperoleh dengan melarutkan 10 ml suspensi dengansejumlah air sehingga diperoleh populasi 7.000, 3.500, 1.750, 875, 438,dan 0 JI/ml air. Masing-masing perlakuan diujikan pada 10 ekor larva,pupa, dan imago dengan menyemprotkan 2 ml suspensi JI. Pengamatanmortalitas B. longissima dilakukan pada 24, 48, dan 72 jam setelahinfestasi (JSI). Hasil penelitian menunjukkan bahwa patogenisitasHeterorhabditis sp. tertinggi pada stadia larva diikuti imago dan pupa.Pada populasi 3.500 JI/ml air kematian larva, imago, dan pupa pada 24 JSIberturut-turut sekitar 73, 63, dan 10%, berbeda tidak nyata denganperlakuan 7.000 JI/ml air. LC 50  Heterorhabditis sp. terhadap larva, imago,dan pupa pada 72 JSI berturut-turut 1.492, 2.622, dan 800.818 JI/ml air.Kata kunci: Brontispa  longissima,  kelapa,  Heterorhabditis  sp.,patogenisitasABSTRACTIn order to evaluate the potensial of Heterorhabditis sp. incontrolling B. longissima bioassays was conducted in IndonesianResearch Institute for Spices and Medicinal Crops, Bogor from November2010 until March 2011. Infective juveniles (IJ) of the nematodes wereharvested by putting dead Tenebrio molitor on a filter paper placed in apetri dish containing 30 ml of water. IJs in the larval body will then go outand live in the water. The suspension was then diluted 1,000 times andthen population counted under a binocular microscope and repeated 3times. IJ population is obtained by dissolving 10 ml suspension to obtain7,000; 3,500; 1,750; 875; 438; and 0 IJs/ml of water. Each treatment wastested on 10 larvae, pupae, and adults by spraying 2 ml IJ suspension.Mortality observations were made at 24, 48, and 72 hours after infestation(HAI). The results showed that the highest Heterorhabditis sp.pathogenicity was found in larvae stadium followed by pupae and adultstadia. In the 3,500 IJ population/ml of water, larvae, imago, and pupaemortalities in 24 HAI were approximately 73, 63, and 10%, respectivelyand were not significantly different with 7,000 IJ/ml of water. LC 50 valuesof Heterorhabditis sp. on the larvae, adult, and pupae in 72 HAI were1,492; 2,622; and 800,818 IJ/ml of water, respectively.Key words: Brontispa longissima, Cocos nucifera, Heterorhabditis sp.,pathogenicity
SAFETY AND EFFICIENCY OF XYLEM WATER TRANSPORT IN TWO CASHEW (Anacardium occidentale L.) STRAINS AT THE SEEDLING STAGE PITONO, JOKO; MAKOTO, TSUDA
Jurnal Penelitian Tanaman Industri Vol 18, No 4 (2012): Desember 2012
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v18n4.2012.156-161

Abstract

ABSTRACTAs cashew trees are grown by transplanting seedlings, the seedlingoften suffers from drought damaged due to prolonged dry season. Previousstudy found that the ability to maintain water transport in xylem related todrought resistant character. To determine whether there was trade-offbetween the ability to maintain water transport in xylem and an efficiencyof water transport, differences in xylem vulnerability to dysfunction,hydraulic conductance, and the relationship to xylem vessel diameter wereexamined in two cashew strains. The xylem vulnerability to dysfunctionwas evaluated by the applied pressure which induced 50% loss of stemhydraulic conductivity (P 50 ). The hydraulic conductance on root, stem, andleaf were determined with High Pressure Flow Meter (HPFM). Variationsin the P 50 values were found between A3-1 and Pangkep, whereas thevalues were 1.75 and 0.50 MPa, respectively. However, since there was nodifference in the hydraulic conductance and the vessel diameter, the trade-off between the ability to maintain water transport in xylem and anefficiency of water transport did not occur in cashew. It was suggested thatgood combination of efficiency and safety of water transport enables A3-1to strongly uptake soil water either in dry or wet season resulting in goodadaptation to drought prone environment, and the P 50 value would besuitable parameter for evaluating drought tolerance of cashew at theseedling stage.Key words: cashew strain, vessel, xylem dysfunction, hydraulicconductance, droughtABSTRAKPengembangan jambu mete secara transplanting sering diikuticekaman kekeringan pada bibit akibat musim kering yang berkepanjangan.Studi awal memperlihatkan bahwa kemampuan xylem mempertahankanfungsi transportasi air merupakan karakter pertahanan penting terhadapcekaman kekeringan. Untuk mengetahui apakah terjadi kompensasi antarakemampuan pertahanan fungsi xylem dan tingkat efisiensi transportasiairnya dilakukan pengujian pada aspek kepekaan fungsi xylem, hantaranhidraulik, dan ukuran vesselnya. Kepekaan fungsi xylem ditentukan darinilai tekanan udara yang menyebabkan kehilangan 50% hydraulicconductance (P 50 ). Nilai hydraulic conductance pada akar, batang, dandaun ditentukan dengan menggunakan metode High Pressure Flow Meter(HPFM). Hasil pengujian menunjukkan terdapat perbedaan nilai P 50diantara dua strain jambu mete yang diuji, yakni secara berturut-turut 1,75dan 0,50 MPa pada strain A3-1 dan Pangkep. Karena tidak disertaiperbedaan pada hydraulic conductance dan ukuran vesselnya, makadisimpulkan tidak ditemukan nilai adanya mekanisme kompensasi antarakemampuan pertahanan fungsi xylem dan tingkat efisiensi pengangkutanair. Hal ini memungkinkan A3-1 tetap dapat menyerap air tanah secaracukup, baik pada musim kering maupun musim basah, dan mampuberadaptasi dengan baik di daerah rawan kekeringan. Dan nilai P 50 dapatdijadikan sebagai parameter representatif untuk evaluasi toleransi bibitjambu mete terhadap cekaman kekeringan.Kata kunci:  strain jambu mete, vessel, fungsi xylem, hydraulicconductance, cekaman kekeringan
VIABILITAS DAN EFEKTIVITAS FORMULA NEMATODA Steinernema sp. TERHADAP HAMA PENGGEREK BUAH KAPAS Helicoverpa armigera HUBNER HERI PRABOWO; IGAA INDRAYANI
Jurnal Penelitian Tanaman Industri Vol 18, No 4 (2012): Desember 2012
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v18n4.2012.151-155

Abstract

ABSTRAKEntomopatogen dari genus Steinernema berpotensi digunakansebagai pengendali berbagai serangga hama, terutama ordo Lepidoptera,seperti  Helicoverpa  armigera.  Penggunaan  Steinernema  untukpengendalian H. armigera akan menguntungkan karena aman terhadaplingkungan, mudah diproduksi massal, toleran terhadap berbagai macampestisida, dapat aktif mencari serangga sasaran, tidak menyebabkanresisten dan resurjensi, serta dapat diaplikasikan dengan alat semprotstandar. Namun, formula pestisida hayati mengandung Steinernema masihsangat terbatas. Tujuan penelitian adalah membuat formula Steinernemasp. yang efektif terhadap hama penggerek buah kapas (H. armigera).Penelitian dilaksanakan di laboratorium Patologi Serangga, BalaiPenelitian Tanaman Pemanis dan Serat, Malang mulai bulan Mei-Juli2010. Larva instar III Steinernema sp. dibuat dalam 6 macam formulaperlakuan dengan bahan pembawa (carrier) berbeda-beda, yaitu (1)suspensi (akuades + sukrosa), (2) pellet-2 (sekam padi), (3) pellet-1(tanah liat + arang), (4) agar + spon, (5) kapsul (Ca-alginat), dan (6)kontrol (akuades). Setiap formula diinokulasikan 10 6 juvenil infektif (JI).Masing-masing perlakuan disusun dalam rancangan acak lengkap (RAL)dengan tiga kali ulangan. Penurunan jumlah juvenil infektif (JI) padasetiap formula yang diamati per minggu selama ± 4 minggu. Isolat yangdigunakan untuk penelitian ini berasal dari Asembagus. Untukmengevaluasi efektivitas formula, larva H. armigera diperlakukan denganJI yang berasal dari masing-masing formula setiap minggu selama empatminggu. Perlakuan ini menggunakan rancangan acak lengkap denganempat ulangan. Jumlah penurunan JI setiap minggu selama empat minggusetelah perlakuan. Persentase JI yang hidup pada pellet-1, suspensi, pellet-2, agar + spon, kapsul, dan kontrol berturut-turut sebesar 53; 12,4; 44;63,8; 17,6; dan 5%. Pada minggu pertama sampai minggu keempat setelahperlakuan terlihat bahwa formula yang paling baik mempertahankan JIadalah agar + spon dan kemudian berturut-turut diikuti oleh pellet-1,pellet-2, kapsul, suspensi. Steinernema sp. yang disimpan selama empatminggu dalam berbagai bentuk formula terhadap H. armigera berkisarantara 80-99%. Formula agar dan spon paling baik untuk menyimpanSteinernema sp. selama empat minggu, karena formula ini memberikantingkat viabilitas dan efektivitas Steinernema sp. paling tinggi.Kata kunci : efektivitas, formula, Helicoverpa armigera, Steinernema sp.,viabilitasABSTRACTEntomopathogenic nematodes genus Steinernema for are potentialto be used as a control for various insect pests, especially ordoLepidoptera, such as Helicoverpa armigera. The use of Steinernema tocontrol H. armigera is beneficial because it is environmentally friendly,easy to produce, tolerant to several pesticides, actively search the targetinsect, does not cause resistance and resurgence, and can be applied byusing standard sprayer. Unfortunately, biological pesticide containingSteinernema is still limited. The study was aimed at formulating abiological agen containing Steinernema sp. to control the cotton bollwormweevil (H. armigera). The experiment was conducted at insect pathologylaboratory, Indonesian Sweetener and Fiber Crops Research Institute,Malang from May to July 2010. Steinernema sp. instar III larvae wasformulated in six different forms such as Pellet-1 (clay+carbon),suspension (distilled water+sucrose), Pellet-2 (rice husk), agar+sponge,capsule (Ca-alginate), and control (distilled water). Each formulation wasinoculated with 10 6 Infective Juvenile (IJ). Each treatment was arranged incompletely randomized design (CRD) with three replicates. Eachformulation observed showed decrease in IJ number every week for + 4weeks. Isolates used for this research were originated from Asembagusexperimental station. For evaluation of the formulation effectiveness, H.armigera larvae was treated using IJ from the formula weekly for fourweeks. On the first week after treatment, the percentages of living IJ inPellet-1, suspension, Pellet-2, agar+sponge, capsule, and control were 53;12.4; 44; 63.8; 17.6; and 5%, respectively. Effectiveness of Steinernemasp. stored for four weeks in various formulations against H. armigeraranged from 80-99%. The best formula of Steinernema sp for storage wasagar+sponge because of its ability to viability and effectiveness ofSteinernema sp.Key words: effectiveness, formulation, Helicoverpa armigera, viability,Steinernema sp.,

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