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Dr. Nuri Nurlaila Setiawan
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Sekolah Ilmu dan Teknologi Hayati, Institut Teknologi Bandung Labtek XI, Jl. Ganesha 10 Bandung 40132 West Java - Indonesia
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3BIO: Journal of Biological Science, Technology and Management
Published by Institut Teknologi Bandung
ISSN : -     EISSN : 26558777     DOI : http://dx.doi.org/10.5614%2F3bio.2020.2.1
Core Subject : Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Immunology & microbiology Neuroscience
3Bio: Journal of Biological Science, Technology and Management is an interdisciplinary peer-reviewed journal in a wide aspect related to the field of life sciences and other related fields of study. The journal aims to promote scientific discourse and disseminate research on various branches and applications of bio-science, biotechnology and bio-based management. This journal invites original empirical research, literature reviews, theoretical or methodological contributions, or short communications on, but not limited to, the following topics: - Ecology and Biosystematics - Microbiology - Genetics and Molecular Biology - Animal Development and Physiology - Plant Development and Physiology - Entomology - Biomedical science - Biochemistry - Agronomy - Forestry - Bioengineering - Bioethics - Management of Biological Resources The journal also invites contributions from other associated disciplines. This journal is an open-access journal. Readers may read, download, copy, distribute, print, search, or link to the full texts of these articles without any charge, provided that readers acknowledge the Creative Commons attached to the articles. All submitted papers are reviewed by at least two referees before being accepted for publication, following a double-blind review process. This journal is delivered in an academic English and rigorously edited to provide a quality standard of a scientific journal. This journal is managed by the School of Life Sciences and Technology, Institut Teknologi Bandung, Indonesia.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 5 Documents
 1 
Search results for , issue "Vol. 3 No. 1 (2021)" : 5 Documents clear
Analysis of MeEf1A6 Gene Promoter Activity with In-vitro and In-vivo using Transient and Stable Expression Techniques in Tobacco Plant (Nicotiana tabacum) Galih Gibral Andalusia; Sony Suhandono; Ima Mulyama Zainuddin
3BIO: Journal of Biological Science, Technology and Management Vol. 3 No. 1 (2021)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2021.3.1.1

Abstract

The promoter is a part of the gene that functions in carrying out the gene expression, and its work activity becomes a matter of concern to ensure that expression works effectively. MeEF1A6 (Manihot esculenta Elongation Factor 1 Alfa - 6) is a promoter derived from cassava plants (Manihot esculenta). In previous studies, the MeEF1A6 promoter was successfully isolated, introduced, and characterized into the pBI121 plasmid, replacing the CaMV35S promoter. This study aims to analyze the activity of MeEF1A6 promoters in-vivo and in-vitro by using transient and transgenic techniques in tobacco plants. The pBI121 plasmid containing the MeEF1A6 promoter was introduced into Agrobacterium tumefaciens strain AGL1 and LBA4404. The promoter's work was then analyzed by the result of introducing it into the tobacco plant using the transient and stable transformation. The whole part of explants was used for transient study and tested in a minimum of two biological replicates. Sixty sheets of explant leaves that have been cut were used for stable transformation. The promoter work analysis was carried out with the GUS gene expression that integrated with the promoter with histochemical GUS assay. The transient produced a blue color in the roots, stems, and leaves on the whole repetition. The transverse incision in the stem shows the blue color on the epidermis and procambium tissue. Stable transformation using AGL1 as vector produced 43 shoots from 40 calli. A total of 43 shoots were selected with antibiotics and produced 27 plantlets that were successfully grown. Some plantlets are then reacted with x-gluc as histochemical GUS assay substrat and produced a blue color in the explants, indicating that the MeEF1A6 promoter has been successfully introduced. The results indicate that the MeEF1A6 promoter could work on plant tissue in roots, stems, leaves, and tissues that connect meristems such as procambium in tobacco plants. This reinforces the suspicion that the MeEF1A6 promoter performs work constitutionally as a constitutive promoter.   
Analysis of Bacterial Community level Physiological Profiling on the Fermentation of Traditional Pliek u using BIOLOG EcoPlates Suji Edwar; Pingkan Aditiawati
3BIO: Journal of Biological Science, Technology and Management Vol. 3 No. 1 (2021)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2021.3.1.3

Abstract

Pliek u is an Acehnese traditional condiment made from fermented coconut (Cocos nucifera) endosperm. The traditional pliek u fermentation process typically involves a diverse bacterial community. This research aimed to discover the physiological profile of the bacterial community diversity in pliek u fermentation. BIOLOGTM EcoPlates was used to obtain the physiological functions of the bacterial community during the pliek u fermentation process. The bacteria were then isolated from EcoPlate substrate to determine the predominant microorganism. Results from the analysis showed that the value of the Average Well Colour Development (AWCD) increased during the EcoPlates incubation period. The AWCD values in sample IV were higher than the AWCD values in samples I, II, and III. PCA analysis showed that the use of EcoPlate substrate by the bacterial community at the beginning of the fermentation was correlated with the substrate groups of carbohydrate and polymer, and with the substrate groups of carbohydrate and the amino acid at the end of the fermentation. The phylogenetic analysis showed that EC1 had a close relation with Pseudomonas azotoformans strain NBRC, while EC3 had a close relation with Psedomonas lundensis strain ATCC 49968. In conclusion, there were changes in the use of EcoPlate substrate and the activities of the bacterial community during the pliek u fermentation process.
CO2 Emission and Absorption Estimation in Bandung City by Implementing CO2 Emission Rate Reduction Simulation Using the Stella Program Natalia Christiani; Yayat Hidayat; Sutrisno Trisno
3BIO: Journal of Biological Science, Technology and Management Vol. 3 No. 1 (2021)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2021.3.1.4

Abstract

Bandung CO2 emissions continue to increase in line with its population. The emissions source comes from the industrial, transportation, Liquefied Petroleum Gas (LPG), household, and livestock sectors, whereas CO2 absorption only comes from vegetation through photosynthesis. High CO2 emissions could decrease air quality and reduce environmental health. This study aims to estimate the amount of CO2 emissions and their absorption in Bandung by implementing CO2 Emission Rate Reduction Simulation (CERRS). The simulation comprises four scenarios, namely substitution of vehicle fuel and the application of smart driving techniques, optimization of waste processing in IWPS, processing 90% of livestock waste into biogas, and green space development of 30% of Bandung City area. Estimated CO2 emission and absorption rates were calculated for the next 10 years (2021-2030) using the Stella program version 9.0.2. The results showed that without implementing the CERRS, the amount of CO2 emissions in Bandung in 2030 was estimated to reach 10,983,666.82 tons while implementing the CERRS was 2,361,721.30 tons. Without implementing the CERRS, the estimated amount of CO2 absorptions in 2030 was 214,235.11 tons, while implementing the CERRS was 2,785,703.11 tons. It is expected that the application of the CERRS could reduce the level of CO2 emissions in Bandung by 78.5% and increase CO2 absorptions by 1,200.3%.
Selection of Indonesian Medicinal Plant Active Compounds as Inhibitor Candidates of Oncoproteins E6 and E7 Human Papillomavirus Type 16 by Molecular Docking Riyanti Weni Syafitri; Azzania Fibriani; Reza Aditama
3BIO: Journal of Biological Science, Technology and Management Vol. 3 No. 1 (2021)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2021.3.1.2

Abstract

Cervical cancer cases caused by infection with Human Papillomavirus (HPV), especially HPV 16 (60.5% of cases) continue to increase every year with a high mortality rate. The current anti-cancer drugs were not only specifically targeting cancer cells, but healthy cells and can cause serious side effects. Therefore, it is necessary to find safer alternative therapies, e.g., using active compounds from natural products. The purpose of this study was to find the active compounds of Indonesian medicinal plants potentially as an inhibitor of oncoprotein E6 and E7 HPV 16, the main protein causing cervical cancer by in silico method. In this study, 711 active compounds from 187 medicinal plant species were selected based on molecular weight, solubility, gastrointestinal absorption index, and drug-likeness. Compounds that meet the criteria were tested for their affinity and interaction profile with E6 and E7 proteins through the molecular docking method. The results of this study showed 164 compounds that met the criteria. The molecular docking analysis showed nine of the most potent compounds as E6 inhibitors on the E6AP binding site and six compounds on the p53 binding site. Besides that, there were eleven most potent compounds as E7 inhibitors.  The results of this study indicate that there are natural compounds that can inhibit E6 and E7 proteins and have further potential to be used as anti-HPV drugs. However, further research is needed to test these compounds in vitro and in vivo.
Optimal Feeding Frequency on the Growth Performance of Whiteleg Shrimp (Litopenaeus vannamei) during Grow-out Phase Alif Ihsanario; Ahmad Ridwan
3BIO: Journal of Biological Science, Technology and Management Vol. 3 No. 1 (2021)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2021.3.1.5

Abstract

Whiteleg shrimp (Litopenaeus vannamei) is a fisheries commodity that has experienced a vast increase in production since the early 1970s. As one of the largest contributors to the global shrimp market, Indonesia always tried to increase its shrimp production. To fulfill the global market demand, whiteleg shrimp farmers have met with countless obstacles, e.g., sub-optimal growth problem. Optimal feed management is one of the determining factors that account for the growth and production efficiency of whiteleg shrimp. Feed management practices include feeding frequency, methods of feed monitoring, and controlling. This literature review aims to provide an insight into the optimal feeding frequency for the growth, survival, and feed efficiency of whiteleg shrimp production. The method used for this review was a narrative review approach following the PRISMA scheme for literature sorting, which includes identification, screening, eligibility test, and inclusion. There were eight primary literatures from journals with H indices of 18, 50, 55, 72, 80, and 164 (four articles from Q1 journals and three from Q2 journals) and one article without index. Data analysis revealed that the growth rate was significantly affected by the feeding frequency of on-demand feeding system (AQ1) (P < 0.05), although no significant difference was found in regards to the survival rate and the FCR (P > 0.05). The optimal feeding frequency for industrial-scale shrimp production was found in the on-demand feeding system (AQ1).

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