Garuda - Garba Rujukan Digital

Development Of Appropriate Technology Adoption For Coffee Farmers In Sukawangi Village, Sumedang Bentang Arief Budiman; Hari Purnama; Husna Nugrahapraja; Muhammad Abdur Rasyid; Natas Setiabudhi Daryono Putra; Sri Aswin Suryani; Annisa Ratna Nurillah; Ima Mulyama Zainuddin; Rindia Maharani Putri; Wervyan Shalannanda; Raden Aswin Rahadi
Jurnal Ilmu Sosial Politik dan Humaniora (Jisora) Vol 2 No 2 (2019): Jurnal Ilmu Sosial Politik dan Humaniora
Publisher : Fakultas Ilmu Sosial dan Ilmu Politik Universitas Garut

In this paper, the authors propose solutions to increase the added value of coffee in the form of the process of making the process of making coffee using appropriate technology adoption. This paper uses qualitative methods of locational survey and literature study. Survey activities are conducted in the form of interviews and observations. The team conducted interviews with coffee farming communities in the area and observed the conditions of coffee farming. The literature study was conducted by synthesizing literature reviews and continued by formulating the theory and principles of appropriate technology in the context of local agriculture in Sukawangi Village, Sumedang Regency, West Java. In this paper, the authors describe the solution proposals related to the problems of coffee farmers in Sukawangi, Sumedang. Two suggestions for increasing the value added of coffee beans are by using appropriate technology adoption. With both of these solutions, it is expected that the quality of coffee plantation in Sumedang can be increased and can be marketed in both domestic and international markets. As a result, the welfare of farmers in Sumedang will increase.

Analysis of MeEf1A6 Gene Promoter Activity with In-vitro and In-vivo using Transient and Stable Expression Techniques in Tobacco Plant (Nicotiana tabacum) Galih Gibral Andalusia; Sony Suhandono; Ima Mulyama Zainuddin
3BIO: Journal of Biological Science, Technology and Management Vol. 3 No. 1 (2021)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2021.3.1.1

The promoter is a part of the gene that functions in carrying out the gene expression, and its work activity becomes a matter of concern to ensure that expression works effectively. MeEF1A6 (Manihot esculenta Elongation Factor 1 Alfa - 6) is a promoter derived from cassava plants (Manihot esculenta). In previous studies, the MeEF1A6 promoter was successfully isolated, introduced, and characterized into the pBI121 plasmid, replacing the CaMV35S promoter. This study aims to analyze the activity of MeEF1A6 promoters in-vivo and in-vitro by using transient and transgenic techniques in tobacco plants. The pBI121 plasmid containing the MeEF1A6 promoter was introduced into Agrobacterium tumefaciens strain AGL1 and LBA4404. The promoter's work was then analyzed by the result of introducing it into the tobacco plant using the transient and stable transformation. The whole part of explants was used for transient study and tested in a minimum of two biological replicates. Sixty sheets of explant leaves that have been cut were used for stable transformation. The promoter work analysis was carried out with the GUS gene expression that integrated with the promoter with histochemical GUS assay. The transient produced a blue color in the roots, stems, and leaves on the whole repetition. The transverse incision in the stem shows the blue color on the epidermis and procambium tissue. Stable transformation using AGL1 as vector produced 43 shoots from 40 calli. A total of 43 shoots were selected with antibiotics and produced 27 plantlets that were successfully grown. Some plantlets are then reacted with x-gluc as histochemical GUS assay substrat and produced a blue color in the explants, indicating that the MeEF1A6 promoter has been successfully introduced. The results indicate that the MeEF1A6 promoter could work on plant tissue in roots, stems, leaves, and tissues that connect meristems such as procambium in tobacco plants. This reinforces the suspicion that the MeEF1A6 promoter performs work constitutionally as a constitutive promoter.

Effect of PGPM and goat manure doses on biomass yield and rosmarinic acid content in cat's whiskers plant (Orthosiphon aristatus Bl. Miq) Ima Mulyama Zainuddin; Millennio Salsabil; Mohammad Ghifary Muktabar; Dadang Sumardi
Current Research on Bioscences and Biotechnology Vol. 4 No. 2 (2023)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2023.4.2/LIGQIUMZ

Cat’s whiskers plant (Orthosiphon aristatus B. Miq) is a medicinal plant containing various metabolites that act as antioxidants and anti-inflammatory, including rosmarinic acid. The domestic market demand for dry leaves was 20-40 tons and abroad it was 141-180 tons in the 1990-2005 period, while production from 2013 to 2017 decreased from 116 tons to 56 tons. Therefore, it is necessary to increase the cat's whiskers biomass production, one of which can be done through the engineering of growing media using organic fertilizers, including goat manure and microbes, e.g., Plant Growth Promoting Microorganisms (PGPM). This study aims to determine the optimal combination of PGPM – Goat Manure for biomass and the rosmarinic acid content in O. aristatus. The study was conducted using a 2-factor randomized block design (RBD) with 12 treatment combinations and 3 repetitions on cultivated land in the Tanjungsari area and analysis of the results was carried out in the SITH laboratory from October 2020 to March 2021. The rosmarinic acid content in the leaves ranged from 144.43 – 206.31 mg/g or 14.44 – 20.63%, with the highest content found in leaves treated with 3 ml/L PGPM and 86.2 g/plant goat manure fertilizer (P2O2).

Development Of Appropriate Technology Adoption For Coffee Farmers In Sukawangi Village, Sumedang Bentang Arief Budiman; Hari Purnama; Husna Nugrahapraja; Muhammad Abdur Rasyid; Natas Setiabudhi Daryono Putra; Sri Aswin Suryani; Annisa Ratna Nurillah; Ima Mulyama Zainuddin; Rindia Maharani Putri; Wervyan Shalannanda; Raden Aswin Rahadi
Jurnal Ilmu Sosial Politik dan Humaniora (JISORA) Vol 2 No 2 (2019): Jurnal Ilmu Sosial Politik dan Humaniora
Publisher : Fakultas Ilmu Sosial dan Ilmu Politik - Universitas Garut

In this paper, the authors propose solutions to increase the added value of coffee in the form of the process of making the process of making coffee using appropriate technology adoption. This paper uses qualitative methods of locational survey and literature study. Survey activities are conducted in the form of interviews and observations. The team conducted interviews with coffee farming communities in the area and observed the conditions of coffee farming. The literature study was conducted by synthesizing literature reviews and continued by formulating the theory and principles of appropriate technology in the context of local agriculture in Sukawangi Village, Sumedang Regency, West Java. In this paper, the authors describe the solution proposals related to the problems of coffee farmers in Sukawangi, Sumedang. Two suggestions for increasing the value added of coffee beans are by using appropriate technology adoption. With both of these solutions, it is expected that the quality of coffee plantation in Sumedang can be increased and can be marketed in both domestic and international markets. As a result, the welfare of farmers in Sumedang will increase.

Title

Found 4 Documents
Search

Abstract

Abstract

Abstract

Abstract

Title Search

Found 4 Documents Search

Abstract

Abstract

Abstract

Abstract

Title

Found 4 Documents
Search