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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 80 Documents
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Search results for , issue "Vol. 13 No. 2 (2025): June" : 80 Documents clear
Urine Analysis of Cat Utilizing the Thinka® Automatic Urine Analyzer as an Indicator for Uropoetic Disorders Riwu, Katty Hendriana Priscilia; Karina, Soca; Afnani, Daniah Ashri; Yansri, Alifianita Anake; Kholik, Kholik; Khairullah, Aswin Rafif
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14738

Abstract

This study aims to determine the chemical content of the urine of local cats living freely around Mataram. The sample used was cat urine taken randomly. Twenty-five cats’ urine samples were put into a sterile urine pot. Urine chemistry examination and observation were carried out using a urine analyzer strip to identify the levels of urine chemistry, namely nitrite, protein, pH, blood, ketones, bilirubin, and glucose. The examination was done by dipping the strip into each urine sample; the strip was taken and tapped on the tissue, then inserted into the urine analyzer machine. After the results are obtained, the machine will automatically issue the results of the urine chemistry analysis, reading the results for no more than 60 seconds. This study emphasizes observing the quantity or amount of substances and sedimentation in cat urine samples. All of the 25 samples examined were positive (100%) for nitrite, protein, ketones, and glucose levels. The results of the blood level examination in urine were negative in all samples, the examination of urine pH levels in 25 samples varied with an average pH of 6.84, and the examination of bilirubin levels was positive in 24 (96%) urine samples. From the examination of the samples, there were indications of disorders of the uropoetic system and bacteriuria in cat urine.
Komunitas Belajar dalam Lesson study untuk Memberdayakan Kognitif dan Psikomotor Siswa pada Pembelajaran Biologi di Luar Kelas Zukmadini, Alif Yanuar; Lestari, Puji; Rochman, Syaiful; Karyadi, Bhakti
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14844

Abstract

This study aims to enhance cognitive and psychomotor abilities through outdoor learning, designed and evaluated collaboratively by the learning community within the framework of the Lesson study. The study was conducted on grade X students of MAN Insan Cendekia Bengkulu Tengah. Lesson study was carried out in two cycles, each consisting of the plan, do, and see stages. The "plan" stage is the planning stage to prepare the learning tools and strategies that will be carried out. The "do" stage is the implementation of the learning that has been designed. The "see" stage is a reflection of the learning activities that have been carried out. The instruments used consist of observation sheets and test paper. Data is analyzed by calculating the average percentage of each observation indicator, while cognitive and psychomotor learning outcomes are analyzed by calculating the average value obtained. Implementation of the lesson study achieved a percentage score of 100%. Cognitive learning outcomes from cycle I to cycle II increased by 20.43%. Meanwhile, psychomotor learning outcomes increased by 26.79%. The ecosystem acts as a direct learning context that does not only present objects of study to be studied but can also facilitate students to develop conceptual understanding and scientific skills simultaneously. Thus, the reflection carried out will encourage teachers to make continuous improvements. The conclusion of this study is that lesson study activities involving the role of the learning community can improve the planning, implementation, and reflection of biology learning outside the classroom, thus having a significant impact on improving students' cognitive and psychomotor learning outcomes.
Pengembangan E-LKPD Berbasis Problem Based Learning (PBL) Terintegrasi Al-Quran dan Hadis Pada Materi Keanekaragaman Tumbuhan Putri, Adinda Opmila; Ulfa, Syarifah Widya
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14900

Abstract

This study aims to develop a digital student worksheet (E-LKPD) utilizing Liveworksheet, based on Problem-Based Learning (PBL) and integrated with values from the Qur'an and Hadith, focused on the topic of Plant Diversity. The research employed a Research and Development (R&D) approach using the ADDIE model (Analysis, Design, Development, Implementation, Evaluation). Research instruments included expert validation questionnaires for content and media, teacher and student response questionnaires to assess practicality, as well as pre-test and post-test instruments to evaluate effectiveness. The trial subjects were students from classes X MIPA-1 and X MIPA-2 at SMA MAL Medan, involving 60 students and one biology teacher. Validation results showed that the developed E-LKPD was rated as highly relevant by content experts (93.75%) and media experts (97.41%). Practicality assessment yielded scores of 85.7% by the biology teacher and 93.5% by the students, both categorized as very practical. Effectiveness testing showed a learning outcome improvement of 87.4%, indicating a high level of effectiveness. Thus, the PBL-based E-LKPD integrated with Qur'anic and Hadith values on the topic of Plant Diversity is valid, practical, and effective, making it suitable for implementation in high school biology instruction.
Pengembangan Aplikasi Mobile Plant-tagging ‘Pelurutaloka’ sebagai Media Pembelajaran Biologi Keanekaragaman Hayati Hidayat, Muhammad Luthfi; Mahatama, Duta Saksena; Suryani, Titik; Sari, Siti Kartika
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14948

Abstract

This research aims to develop a web-based mobile plant-tagging application that connects local plant species to scientific information through QR codes, which emphasizes contextual, technology-assisted learning grounded in local potential. The resulting educational media, titled Pelurutaloka (an acronym for Pembelajaran Luar Ruang Tanaman Lokal, or Outdoor Learning of Local Plants), is specifically designed to be pedagogically relevant and to serve as a foundation for future studies in local natural resources.This study adopts a Research and Development (R&D) methodology using the 4D model (Define, Design, Develop, Disseminate), with development limited to the evaluation/revision stage. The evaluation phase employed a 14-item Likert-scale questionnaire, which demonstrated high levels of validity and reliability based on expert review and Rasch model analysis (reliability = 0.72). Further Rasch analysis revealed a unidimensionality value of 40.2%, exceeding the acceptable threshold of 20%, indicating that the instrument effectively measured a single construct. The primary contribution of this research lies in the creation of a web-based, mobile-accessible biology learning media designed for outdoor contexts, offering an engaging, technology-enhanced learning experience that promotes student activity and interaction with the environment. This study recommends broader dissemination of the Pelurutaloka platform and its implementation in outdoor biology education, particularly through collaborative, active learning models such as problem-based learning (PBL) and project-based learning (PjBL).
Analisis Penerapan Nilai Pendidikan Karakter Pada Pembelajaran Biologi Era Society 5.0 Pada Siswa Kelas X MAN Binjai Nadia, Rizka Lucy; Ulfa, Syarifah Widya; Suryani, Ira
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15006

Abstract

This study aims to analyze the application of character education values in biology learning in the era of society 5.0 for class X students of MAN Binjai. This study uses a mixed methods approach with a sequential exploratory design, namely a combination research method that combines qualitative and quantitative research methods sequentially. Data collection techniques are carried out using case studies and experiments. Case studies use instruments, namely documentation, interviews and observations of biology teachers. While the experiment uses a student needs questionnaire instrument. The results of the study indicate that the application of character education values in biology learning in the era of society 5.0 for class X students of MAN Binjai that have been developed, in character education the highest score with a value of 89% in the aspect of tolerance character values, a medium score with a value of 60% in the aspect of health care character values and the lowest score with a value of 42% in the aspect of environmental care. The obstacles faced by biology teachers are that students still do not carry out the tasks and obligations given by the teacher with full responsibility, students do not submit assignments on time.
Pengembangan Instrumen Three-Tier Test Diagnostik pada Materi Sistem Koordinasi untuk Mengidentifikasi Miskonsepsi Peserta Didik di SMAN 2 Sinjai Ariya, A. Aida; Muis, Abd.; Ismail, Ismail
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15044

Abstract

The aim of this study was to develop a diagnostic test instrument and to identify the level of misconceptions held by students regarding the topic of the coordination system. The development design of this research involved trials with Grade XI students at SMAN 12 Sinjai and field testing with Grade XI students at SMAN 2 Sinjai. The methods employed included test item development, interviews, and documentation. The resulting instruments consisted of test blueprints, test items, and answer keys. The test items underwent expert validation and were subjected to reliability testing, difficulty level analysis, and item discrimination index evaluation through all trial stages (one-to-one, small group, and field testing). Out of 30 test items that were validated, only 21 advanced to the final stage, namely the field test. The findings revealed that, on average, 40.47% of the 50 students experienced misconceptions, which were categorized into two main indicators. The highest misconception rates in the coordination system topic were found in the indicators related to the structure of the central and peripheral nervous systems, as well as the structure and function of sensory organs, with an average misconception rate of 56% for the relevant test items. Based on the results of developing the three-tier diagnostic test on the coordination system material, it can be concluded that the instrument is effective and appropriate for identifying students' misconceptions on the topic.
Pengetahuan Etnobotani Tumbuhan Obat Pada Penderita Penyakit Lambung di Desa Trusmi Kulon, Kabupaten Cirebon Mahrani, Aura Sutra; Husain, Fadly
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15047

Abstract

This study aims to understand the ethnobotanical knowledge possessed by the people of Trusmi Kulon Village, especially in the utilization of medicinal plants for people with gastric diseases. This research used a qualitative method with an ethnographic approach. Data collection in this study was carried out through in-depth interview methods, with a question model using semi-structured interview techniques. Interviews were conducted with 13 informants from the general public in Trusmi Kulon Village who were selected based on their knowledge of ethnobotany and history of gastric disease. The researcher also used the PlantNet application to help identify plants. Data analysis used the Miles and Huberman model, which consists of data reduction, data presentation, and conclusion drawing. The results showed that the people of Trusmi Kulon Village have knowledge about the use of medicinal plants that have been passed down from generation to generation. Overall, 34 medicinal plants were found that are believed to be efficacious, but specifically there are 9 medicinal plants used by the community in overcoming gastric diseases. Plant parts used in making medicinal herbs include leaves, flowers, stems, seeds, and rhizomes. The process of processing plants into medicine varies, ranging from boiled, grated, squeezed, burned, pounded, dried and then brewed, rubbed, or eaten directly. Each herb has a different processing method, some are single, some need to be mixed with other ingredients. This finding shows that traditional medicine is still an important part of society as an alternative treatment that is trusted for generations.
Detection of HSP70 in Working Horses Using ELISA on Sumbawa Island Haddar, Mariyam Al; Pradana, Munawer; Kholik, Kholik; Supriadi, Supriadi; Satuman, Satuman
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15197

Abstract

Heat shock protein 70 also known as HSP70 is a protein that regulates in response to stress factors such as heat, exercise, and others. The current research work used enzyme-linked immunosorbent assay (ELISA) to measure HSP70 levels in working horses on Sumbawa Island. Blood was taken through jugular venipuncture whereas saliva was collected non-invasively via cotton swab, both were analyzed with ELISA. The results indicated different expressions of HSP70; saliva shows a significantly higher level of HSP70 (52.07 ± 25.25 ng/mL) than serum (43.71 ± 34.83 ng/mL) from the same animal. The observed differences might indicate a better representation of acute stress by saliva due to physiological rapid turnover compared to serum, which reflects an integration of cumulative stress exposure. Our finding thus proves that double monitoring of both biological matrices offers complementary information in assessing stress in working horses while taking salivary HSP70 into account as especially promising for the in-field welfare monitoring due to its non-invasive collection and sensitivity to immediate stress conditions.
Uji Daya Hambat Antibakteri Produk Sabun Cuci Tangan Ekstrak Kulit Kakao (Theobroma cacao L.) Pada Bakteri Patogen Dewi, Annisa Mulya; Priantari, Ika; Munandar, Kukuh
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15237

Abstract

This study aims to explore the effectiveness of handwashing soap made from cocoa pod husk extract in inhibiting the growth of pathogenic bacteria such as Salmonella typhi, Staphylococcus aureus, and Escherichia coli, which are common causes of various diseases. The extract used was a crude cocoa pod husk extract obtained through the maceration method using distilled water (aquadest) as the solvent. The extract was then formulated into soap at concentrations of 80%, 90%, and 100%. Antibacterial activity tests were carried out using the disk diffusion method based on CLSI standards to measure the inhibition zones against Escherichia coli, Staphylococcus aureus, and Salmonella typhi. The study used a completely randomized factorial design (3 x 3), involving three extract soap concentrations and three types of bacteria, each tested in three replications. Data were analyzed using Analysis of Variance (ANOVA) to determine the significance of differences among treatments. The inhibition zone measurements showed that at concentrations of 80%, 90%, and 100%, the zones for E. coli were 4.1 mm, 4.9 mm, and 6.3 mm respectively, classified as weak to moderate. For S. aureus, the inhibition zones were 7.3 mm at 80%, 11.30 mm at 90%, and 13.77 mm at 100%, which fall into the moderate to strong category. For S. typhi, the inhibition zones were 5.16 mm (80%), 6.3 mm (90%), and 8.81 mm (100%), categorized as moderate. It can be interpreted that cocoa pod husk extract soap showed the most effective antibacterial activity against S. aureus at 90% and 100% concentrations, while its inhibitory effect on E. coli and S. typhi was weaker to moderate. Biologically, the effectiveness of cocoa pod husk extract soap as a natural antibacterial agent is more optimal against Gram-positive bacteria such as S. aureus, whereas its effectiveness against Gram-negative bacteria like E. coli and S. typhi still needs to be optimized. The ANOVA test results showed a p-value < 0.05, indicating that the average inhibition zone results among the three bacterial types differed significantly. This confirms the potential of cocoa pod husk as a natural raw material for producing environmentally friendly antibacterial hand soap.
Optimasi Sterilisasi Eksplan Biji Manggis (Garcinia mangostana L.) Menggunakan Natrium Hipoklorit Secara In vitro Harahap, Fauziyah; Asri, Elita; Hasairin, Ashar; Edi, Syahmi; Suriani, Cicik; Daulae, Abdul Hakim; Panggabean, Nurul Huda
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15281

Abstract

This study evaluates the effects of NaOCl concentration and immersion time on contamination levels and viability of in vitro mangosteen seed explants, aiming to identify the optimal sterilization protocol. Conducted at YAHDI Tissue Culture Laboratory, Medan, Indonesia, a Randomized Complete Block Design tested three treatment combinations: (1) 15% and 10% NaOCl for 5 minutes, (2) 20% and 15% NaOCl for 15 minutes, and (3) 30% and 20% NaOCl for 25 minutes. After immersion, explants were rinsed three times with sterile distilled water. Observations spanned 12 MST, with data analyzed using ANOVA and Games-Howell post hoc tests; contamination sources were qualitatively described. Results showed the first combination had the lowest effectiveness, the second was better but suboptimal, while the third significantly delayed contamination and yielded the highest explant survival rate of 86.1%. The third treatment also prolonged the contamination-free period compared to others. Fungal and bacterial contaminants were predominant, highlighting the need for effective disinfectants against both. These findings indicate that the third combination is a promising sterilization protocol for mangosteen seed explants, especially for hard endocarp tissues prone to contamination.

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