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Ummy Mardiana Ramdan
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Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)
Published by Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medis Indonesia
ISSN : 27750108     EISSN : 27742504     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology
Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) merupakan jurnal AIPTLMI (Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia) yang dapat diakses secara terbuka (open acces) yang berfokus pada pengembangan ilmu pengetahuan Teknologi Laboratorium Medik (TLM) dengan tujuan untuk memperluas pengetahuan, pemikiran dan penelitian di bidang laboratorium kesehatan. Topik yang diangkat meliputi hematologi, kimia klinik, imunoserologi, imunohematologi, sitohitoteknologi, mikrobiologi, parasitologi, dan toksikologi klinik.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 8 Documents
 1 
Search results for , issue "Vol 3 No 1: April 2022" : 8 Documents clear
PERBANDINGAN HASIL PEMERIKSAAN KONTAMINASI BAKTERI PADA PRODUK TROMBOCYTE CONCENTRATE METODE KONVENSIONAL DAN APHERESIS Resti Ariani; Ria Aidina
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.47

Abstract

Platelet Concentrate (PC) is one of the blood products that is often used for transfusion. The storage temperature for TC products is in the range of 20-24OC which is the optimal temperature for bacterial growth. TC products contaminated with bacteria can cause a dangerous septic reaction. With the development of blood transfusion, apheresis methods emerged for the manufacture and aseptic collection of TC products. The apheresis method is considered safer than the conventional method which has a high chance of bacterial contamination. This study aims to compare the results of the examination of bacterial contamination in conventional TC products and apheresis methods. This study is a comparative descriptive study with a retrospective approach in the form of examining data on bacterial contamination of TC PMI Semarang City during 2019. The results of the examination of the TC apheresis method product showed that there were no TC products that were contaminated with bacteria (0.0%; 0 of 48), while in conventional method, there is 1 TC product that has bacterial contamination (0.3%; 1 out of 360). Fisher exact test on each TC product obtained p value = 1.00 (> 0.05) so that the difference that appears is not significant. Examination of bacterial contamination in conventional TC products and apheresis gave the same good results.
EKTRAK KULIT MANGGIS (Garcinia Mangostana L) SEBAGAI ALTERNATIF PENGGANTI EOSIN UNTUK PEMERIKSAAN TELUR CACING Apriani Apriani; Ereskadi Ereskadi
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.62

Abstract

Direct examination of worm eggs was found to be colorless, making it difficult to see under a microscope. The use of Eosin 2% is a simple method of examining worm eggs commonly used in medical diagnosis and research. Some of the drawbacks of Eosin are high price and its ingredients can damage the environment. Another dye to consider is the mangosteen peel. Mangosteen peel contains anthocyanins which produce a red color. This study aims to see whether mangosteen peel (Garcinia mangostana L) can be used as an alternative for coloring intestinal nematode worm eggs. This study used a descriptive experimental method, namely observation by looking at the contrast, color absorption, and clarity of the egg in the microscope. The concentration used in this study were 1:1; 1:2; 1:3; 1:4 and 1:5. Staining using mangosteen peel (Garcinia mangostana L) at a concentration (1: 2) showed the quality of the staining was close to the quality of the 2% eosin staining (control). This makes mangosteen peel extract (Garcinia mangostana L) can be used as an alternative to eosin in examining worm eggs.
UJI ANTIFERTILITAS EKSTRAK LIDAH BUAYA (Aloe Vera L.) TERHADAP KUANTITAS SPERMATOZOA DAN EKSPRESI CYCLOOXYGENASE-2 PADA TESTIS MENCIT (Mus musculusL.) Rica Vera; Dedy Sambahtra
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.65

Abstract

The family planning program needs to be carried out as an effort to reduce the rate of population growth. In this case the participation of men is very important and need research about natural ingredients that can be used as antifertility drugs. Aloe vera leaf (Aloe vera L.) is one of the plants that have an antifertility effect so that it can be used as a contraceptive for men. The purpose of this study was to determine the effect of ethanol extract  aloe vera leaves of the quantity of spermatozoa and the expression of Cyclooxygenase-2 (COX-2) in the testes of mice (Mus musculus L.). The research used four treatment groups, namely P0 as a control was given aquadest 0,5 mL, P1 was given extract 15 mg/25g body weight by oral, P2 was given extract 20 mg/25g body weight by oral, and P3 was given extract 25 mg/25g body weight by oral given for 4 weeks. The research used completely randomized sampling method and data were analyzed using One Way ANOVA followed by Bonferroni Post Hoc test 5%. Administration of ethanol extract aloe vera leaves resulted in a decreased sperm quantity and increase expression of COX-2 in the testes of mice so that aloe vera leaves had the opportunity as a herbal contraceptive for men.
PENGARUH VARIASI KONSENTRASI SARI PATI BUAH SUKUN SEBAGAI ALTERNATIF MEDIA SEMI SINTETIK PADA PERTUMBUHAN JAMUR Candida albicans Reza Yuliana; M. Taufiq Qurrohman
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.67

Abstract

Gold standard examination Candida sp.  is a culture test.  The abundance natural resources in Indonesia encourages researchers to take advantage the wealth of natural resources to create alternative semi-synthetic media for mushroom growth from breadfruit.  The purpose of this study was determining the effective concentration of alternative semi-synthetic media of breadfruit essence as growth medium for the fungus Candida albicans. This research is experimental study. Observations were made macroscopically and confirmed microscopically. The results showed that average growth of the colonies on PDA media was 15.50, for the alternative breadfruit media the average of colonies was 24.50 (100% concentration), 19.75 (90% concentration), 19.50 (80% concentration), 16.00 (70% concentration), 15.50 (60% concentration). The results of the ANOVA test obtained value <0.05, so H1 was accepted there was a significant difference in the average growth of fungi in the media of breadfruit essence. The conclusion from this research is that the concentration of 60% breadfruit essence is the effective for growing Candida albicans.
HUBUNGAN KADAR KARBOKSIHEMOGLOBIN DENGAN HEMATOKRIT DALAM DARAH MAHASISWA TINGKAT AKHIR TERPAPAR KARBONMONOKSIDA DI SEKOLAH TINGGI ILMU KESEHATAN NASIONAL Wijanarko Wijanarko; Mastuti Widi Lestari
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.69

Abstract

Carboxyhemoglobin is a substance formed due to the bonding of carbon monoxide with haemoglobin, which results in a lack of oxygen in the body, causing polycythemia which leads to an increase in blood hematocrit. motorized. This research method uses quantitative. The subjects of this study amounted to 30 people where the subjects were divided into 2 categories, namely smokers and motorcyclists. Data analysis using Shapiro Wilk and Rank Spearman Based on the research, it was found that the average COHb level was 6.06 while the average hematocrit in the normal range obtained p values ??> 0.05 for COHb 0.827, 0.836 and 0.827 while the hematocrit was 0.827, 0.836 and 0.827. This study concludes that there is no relationship between carboxyhemoglobin levels and hematocrit levels in final year students who are exposed to carbon monoxide from cigarette smoke and motor vehicle fumes.
UJI EFEKTIFITAS EKSTRAK DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb) TERHADAP PERTUMBUHAN BAKTERI Streptococcus mutans Siti Juariah; Junia Wiranda; Harni Sepryani
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.75

Abstract

Fragrant pandan leaves (Pandanus amaryllifolius Roxb) is a type of plant that contains flavonoid compounds, alkaloids, saponins and tannins that can function as antibacterial. Streptococcus mutans is one of the bacteria that causes dental caries. Fragrant pandan leaf extract is expected to be an alternative to overcome bacterial infections such as dental caries. Dental caries is a disease caused by damage to the enamel layer that can extend to the nerves of the teeth so that it will cause cavities caused by the bacterium Streptococcus mutans. The aim of this study was to determine the effectiveness of fragrant pandan leaf extract against the growth of Streptococcus mutans bacteria that causes dental caries. This type of research is a laboratory experiment using the disc diffusion method. Fragrant pandan leaves were extracted by maceration method using 96% ethanol as solvent. The results of this study showed that the pandan fragrant leaf extract had an inhibitory effect on the growth of Streptococcus mutans bacteria at concentrations of 25%, 50%, and 75% with an average inhibition zone diameter of 10.3 mm, 13.3 mm, 17.6. mm. Positive control (amoxicilin) ??44.3 mm and negative control (aquadest) had no value to inhibit bacterial growth. The conclusion is that pandan leaves extract (Pandanus amaryllifolius Roxb) can inhibit Streptococcus mutans which is the bacteria that causes dental caries.
PRESISI DAN AKURASI HASIL QUALITY CONTROL PADA PARAMETER PEMERIKSAAN GLUKOSA DARAH DI LABORATORIUM KLINIK RUMAH SAKIT X KOTA TASIKMALAYA Meti Kusmiati; Rianti Nurpalah; Resa Restaviani
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.86

Abstract

quality system is running properly and is carried out ensuring laboratory examination results. The purpose of this study was to see an how of the results of the precision and accuracy of the quality control parameters of blood glucose examination and to find out the mean, SD, CV and d% results. The research method used is descriptive quantitative analytic. The results obtained were quality control examinations of blood glucose in August CV (3.65%) d% (4.0%), September CV (2.96%) d% (4.5%), October CV (3.35%) d% (3.9%), November CV (2.0%) d% (3.5%), December CV (3.03%) d% (2.6%) and January CV (4.18%) d% (4.9%). The Ministry of Health has decided that limit maximum CV for checking blood glucose is still below the target value, namely <5%. The bias value (d%) of the blood glucose test was still below the target value set by ISO 15197, namely <5%. The results of this study it can be concluded that the maximum CV value and the bias value (d%) are still below the target value set by the Ministry of Health and ISO 15197, namely < 5%.
IDENTIFIKASI Plasmodium vivax MENGGUNAKAN METODE NESTED PCR DI WILAYAH ENDEMIS MALARIA PROVINSI NUSA TENGGARA TIMUR Dewi Inderiati; Sarwo Handayani; Danesya Syaeptiani; Ni Putu Aryadnyani
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.96

Abstract

East Nusa Tenggara Province has one of the highest malaria prevalence rates in Indonesia (1.99 percent) and is one of the locations where malaria is expected to be eradicated by 2030. There is a hypnozoite phase in P. vivax that might cause recurrence in patients. Only young erythrocytes are infected by P. vivax, resulting in a low parasite density in the blood. As a result, P. vivax is difficult to detect, necessitating the employment of an appropriate diagnostic procedure. With the advancement of molecular biology, it is now possible to diagnose malaria using the nested PCR method, resulting in more sensitive and specific results. The goal of this study was to identify P. vivax in 135 whole blood samples from people of Oenino District, South Central Timor Regency, East Nusa Teggara Province, using the nested PCR method. In this study, 8 (5.92%) of the samples tested positive for Plasmodium sp., with 5 (3.70%) of the samples being P. vivax. Patients with vivax malaria were mostly men (40.0%) and women (60.0%), with ages ranging from 31 to 50 years (40.0%), 41 to 50 years (20.0%), and > 50 years (20.0%). (40.0 % ). A total of three patients (40.0 %) experienced clinical signs of fever and anemia (20.0 %). Based on this results of a P. vivax examination, the PCR approach might be utilized. The discovery of P. vivax in the community needs o be concern for the local government, particularly in terms of malaria treatment monitoring and assessment in order to prevent relapse, increase malaria cases, and promote the effectiveness of malaria elimination initiatives.

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