Berkala Penelitian Hayati
Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.
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<![CDATA[IDENTIFIKASI KARAKTER MORFOLOGI NEMATODA SISTA PADA TANAMAN JAGUNG (Heterodera zeae) DI INDONESIA ]]>
<![CDATA[Yuliantoro Baliadi]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/292
<![CDATA[Identification of nematode species is the first step in the resolution of major nematological problems. Accurate identification of maize cyst nematode species is essential for more detailed research, particularly for it control measure. Although morphological observations by using the key characters of each nematode are suitable for identification purposes, the cyst nematodes could not be clearly identified based on it due to the wide variations. For the identification of the cyst nematode species, key characters relating to body surface, head, mouth, esophagus, tail as well as habitat and feeding habits was used in the present examination. Procedures for the permanent and temporary preparations of nematodes were used. The nematode specimens were observed under the microscope to confirm the key characters. Results of measurements and descriptions revealed that the cyst nematode collected from maize roots in Madura island have the same ambifenestrate as that of Heterodera zeae. The underbridge is short and thin and there is two layer of bullae arrangement in vulval cone, the first layer with four finger-like bullae located immediately below the underbridge and the second layer very randomly located below the first one. These characteristics are of H. zeae. These complete of morphological identifications are a new report of H. zeae in Indonesia.]]>
<![CDATA[DEVELOPMENT AND PATHOGENICITY OF INFECTIVE JUVENILES ORIGINATING VIA ENDOTOKIA MATRICIDA IN AXENIC STEINERNEMATID NEMATODES ]]>
<![CDATA[Yuliantoro Baliadi]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/293
<![CDATA[Development and insecticidal activity of axenic infective juveniles (IJs) originating from endotokia matricida in maternal bodies of Steinernema glaseri and S. carpocapsae were investigated with a comparison to IJs developed in monoxenic culture. In comparison with the monoxenic steinernematids, the axenic ones grew slower and produced fewer IJs when they were cultured in a sterile chicken liver extract medium supplement with an autoclaved nematode infected Galleria mellonella larva. The phenomena of endotokia matricida, an intra-uterine development of hatched juvenile, occurred in an axenic culture as did the monoxenic ones. Although it occurred faster in monoxenic culture, the ratio of females bearing endotokia matricida was more numerous in axenic ones. These axenic females also produced IJs through the endotokia matricida phenomenon. Compared to the normal IJs develop in monoxenic culture, the IJs originated via endotokia matricida of axenic nematodes showed lower insecticidal activity and it could not reproduce in G. mellonella cadaver.]]>
<![CDATA[EKSPRESI SPERMATOGENESIS TIKUS PUTIH SETELAH INDUKSI DENGAN PROTEIN INSULIN LIKE GROWTH FACTOR–I COMPLEX PLASMA SEMINALIS KAMBING ]]>
<![CDATA[Suherni Susilowati]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/294
<![CDATA[The objective of this research was to analyzed the expression of spermatogenesis on white rat after inducted by Insulin Like Growth Factor – I (IGF-I) Complex protein from goat seminal plasm. This research consisted of two phase. The fi rst phase was explorative laboratory experiment concerned with identification, isolation and spesifi cation IGF-I Complex by using gel Native Polyacrylamid gel electroforesis, electroelution and Western Blot. Result of gel Native PAGE indicated that of goat seminal plasm with Comassie Blue Stain consisted of seven bands. Result of Western Blot indicated that molecule of IGF-I Complex of goat seminal plasm to bound specific with anti IGF-I Complex at protein ribbon. The second phase concerned with expretion of spermatogenesis on white rat after induction with isolat of IGF-I Complex protein. Result of the expression spermatogenesis on white rat after induction IGF-I Complex to show the signifi cant different between three groups (p < 0.05). From result above can be concluded that IGF-I Complex protein can increase the total of spermatocyte I and spermatocyte II.]]>
<![CDATA[KEANEKARAGAMAN JENIS ANGGREK PULAU WAWONII ]]>
<![CDATA[Diah Sulistiarini]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/295
<![CDATA[An exploration and inventory of orchid plant was carried out in Wawonii Island, South-east Celebes. The aim of the study was to know about orchid diversity in the area, because no fi eld survey conducted since 1940. For that reason all orchid species fi nding were collected as life collection for planted in the Bogor Botanical Garden and dried collection for stored in the Herbarium Bogoriense. Ninety one orchid species were recorded and 36 species of them were new record of Celebes. Out of 1 species Trichoglottis javanica J. J. Sm. was recognized as endemic of Java. Five species orchid were endemic in Celebes, there are Bulbophyllum balapiuense J.J.Sm., Hyppeophyllum celebicum Schltr., Oberonia exaltata Schltr., Thrixspermum celebicum Schltr. and Malaxis lombasangensis (J.J.Sm.) S. Thomas Schut. & de Vogel.]]>
<![CDATA[NERACA EKOLOGI PENAMBANGAN TIMAH DI PULAU BANGKA Studi Kasus Pengalihan Fungsi Lahan di Ekosistem Darat ]]>
<![CDATA[Eddy Nurtjahya]]>;
<![CDATA[Fournita Agustina]]>;
<![CDATA[Wike Ayu Eka Putri]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/296
<![CDATA[Unequal understanding among tin mining stake holders on trade regulation often triggered social conflict and therefore it was suggested to build political will especially among national, provincial, and regencial/municipal government levels. One important effort to bring out political will is presenting an independent and scientific study on comparision between socio-economic and environmental impact of tin mining. Purposive sampling was conducted to inconventional miners at reserved forest at Lubuk Kelik hamlet, pepper farmers at Silip village, and rubber farmers at Bencah village. Tin mining changed soil properties and microclimate, reduced vegetation composition and structure, arbuscular mycorrhizal fungi, phosphate solubilizing microorganism, ants, and Collembola population. Land conversion increased and contributed up to 95% income. The welfare improvement, however, was apparent as the recovery cost was neglected by inconventional tin miners, and only pepper land conversion showed benefit. Inconventional tin mining regulation needs improvement in order to increase land use efficiency and recovery cost. Understanding on ecological balance of more people and for more sustainable social welfare should be improved as well.]]>
<![CDATA[MENINGKATKAN PRODUKSI FLAVAN-3-OL MELALUI KALUS Camellia sinensis L. DENGAN ELISATOR CU2+ ]]>
<![CDATA[Sutini B]]>;
<![CDATA[Tatik W]]>;
<![CDATA[Wahyu W]]>;
<![CDATA[Sutiman B. Sumitro]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/297
<![CDATA[Flavan-3-ol is a secondary metabolite in tea plant that is used as anti obesity agent. The difficulty in obtaining Flavan-3-ol out of tea plant is due to dependency on season, need for large land, need for very intensive care and relatively low production. Therefore flavan 3-ol production needs tobe developed in vitro culture technique. This technique can cope with the handicapes above. It can effectively control production and requires less space. The purpose of this research was to enhance fl avan 3-ol production by modifyingmedium and precursor appropriately. The steps of this process were: (1) Callus induction through cultivating the tea shoot explants on medium fi lled with some growth hormone, (2) fl avan-3-ol induction on callus culture using Cu 2+ elicitor. (3) Observation over callus growth, (4) Observation over qualitative characterizes of flavan 3-ol. The result of the research using Cu 2+ elicitor shows that the production of flavan-3-ol increases by 12.5%.]]>
<![CDATA[PREFERENSI DAN KERUSAKAN TUMBUHAN KOLEKSI KEBUN RAYA CIBODAS OLEH BENALU Scurrula oortiana (Korth.) Dans. ]]>
<![CDATA[Sunaryo Sunaryo]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/298
<![CDATA[The mistletoe of Scurrula oortiana (Korth.) Dans. (Loranthaceae) growing on several collection plants at Cibodas Botanical Garden. The mistletoe parasite 48 species of plants collection, what come within in 24 tribe. Plants collection which attacked as the highest is group of Ficus type (Moraceae). While attacked by individual in number at most is Callistemon citrinus (Myrtaceae). The mistletoe has caused the damage at part of branch. Infected and uninfected branches of host species were observed. The result showed that the mistletoe causes degradation of the distal part of the infected branches. Damage value of branch which parasite of mean more than 50%.]]>
<![CDATA[PERANAN POHON INDUK DAN PENGARUH PEMUPUKAN DAUN TERHADAP POLA PERTUMBUHAN SEMAI CENDANA (Santalum album L.) ]]>
<![CDATA[Albert H. Wawo]]>;
<![CDATA[Fauzia Syarif]]>;
<![CDATA[Budiardjo Budiardjo]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/300
<![CDATA[Sandalwood (Santalum album L.) is one of the economic plants in Indonesia that is threatened rare, because over exploitation and is not cultivated intensively yet by local people in province of Nusa Tenggara Timur (NTT). Therefore need some obviously activities in conservation. One of those activities is producing sandalwood seedlings for cultivation. Sandalwood seeds were collected from mother seed trees. In recent years was found two mother seed trees that grew in 2 locations in Belu District of NTT province were; Tialai location, subdistrict of Tasifeto Timur and Alas locatian, subdistrict of Kobalima. Sandalwood seeds from two mother seed trees was mentioned have been germinated to produced seedling. One of some activities in maintenance of seedling is fertilizing. In 2006 at Kebun Benih Kian Rai Ikun, Belu have been carried out the study of foliar fertilizing on sandalwood seedling from two mother seed trees was mentioned above Three levels dose of Bayfolan had been sprayed every 2 weeks during 10 weeks. The study used Factorial Completely Randomized Design with 3 replications. The results of this study as follow. The growth of sandalwood seedling was infl uenced by mother seed trees whereas foliar fertilizing with Bayfolan was not give significant effect on growth of sandalwood seedling. Seedling growth from both mother seed trees follow the logistic growth model with determination coeffi cient (R2) = > 0.90.]]>
<![CDATA[RESPONS EMBRIOGENESIS MIKROSPORA TANAMAN TEBU (Saccharum spp.) PADA SUHU DAN LAMA INKUBASI CABANG MALAI DI DALAM MEDIUM B ]]>
<![CDATA[Suaib Suaib]]>;
<![CDATA[W. Mangoendidjojo]]>;
<![CDATA[Mirzawan PDN]]>;
<![CDATA[A. Indrianto]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/310
<![CDATA[Microspore embryogenesis under different temperature and time duration pretreatments of spike segments of Saccharum spp., clone PS862 has been studied in in-vitro laboratory of Faculty of Biology Gadjah Mada University, Yogyakarta. Combination pretreatments of a 4 and 34° C of temperatures, and 0, 2, 4, and 7 days duration of incubation of spike segments in the B medium were studied in this observation. A B5 medium of Gamborg supplemented with 2 mgl-1 2.4-D or 2 mg.l-1 NAA was also studied on the formation of proembryos and embryos under 25°C temperature in the dark for at least 30 days. Results of the research showed that the percentage of viable microspores was decreased as simultaneously prolonged of the time of duration in the B medium either under low temperature or under high temperature pretreatments tested. Incubation of spike segments under low temperature after 4 days was decreased the percentage of viable microspores over 44%. In contrast, under high temperature pretreatment the percentage of viable microspores was decreased over 54%. The higher percentage (12.5%) of binucleate microspores was achieved after 7 days incubation of spike segments in the B medium under the low temperature compared to the high temperature (7.14%). Besides, the higher percentage (87.5%) of simmetrical binucleate microspores as proembryo and embryo precursor was obtained after 2 days incubation in the B medium under the low temperature compared to the high temperature (79.0%). Finally, only B5 medium supplemented with 2 mgl-1 of NAA was yielded the proembryos and embryos after 30 days of incubation.]]>
<![CDATA[PENGARUH BEBERAPA FITOHORMON PADA PEMBENTUKAN MUTAN BARLEY (Hordeum Vulgare) DENGAN FENOTIP BIJI ABNORMAL ]]>
<![CDATA[Ira N. Djajanegara]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/311
<![CDATA[Seg8 is the shrunken endosperm mutant occured naturally in barley (Hordeum vulgare). This recessive mutant does not express xenia which indicates that the endosperm phenotypes depend on the genotype of the diploid maternal parents. This mutant provides an opportunity to analyze factors produced by the female parent that affect the seed development process. One of the factors affecting seed development and growth is phytohormones. In vitro spike culture system adopted from wheat spike culture system was used to investigate the involvement of phytohormones in the grain filling process. Phytohormones were applied during 15 days period of culture. Phytohormones concentrations used were as follows abscisic acid 10–4 M, 5x10–5 M, 10–6M, 5x10–7M and 10–7M. Gibberelic acid concentrations were 5x10–3 M, 5x10–4 M, 5x10–5 M, 2.5x10–5M, 10-5M and 2x10–6M. Cytokinin concentrations were 2x10–6 M, 2x10–7 M and 2x10–8 M. In this experiment, 2 days before anthesis is the best stage to start the spike culture period and 15 days period of culture is sufficient to observe the grain filling process and emergence of the mutant phenotype. The phytohormones treatments as well as their respective concentrations were not able to recover the normal phenotype. Abscisic acid treatment at 5x10-7 M were able to increase the mutant grain dry weight significantly compared to untreated culture but the normal phenotype was not recovered. This indicates that lack of phytohormones was not the maternal factor affecting the seed development process in this particular mutant.]]>
