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All Journal Media Kedokteran Hewan Universa Medicina AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Ovozoa: Journal of Animal Reproduction LOSARI: Jurnal Pengabdian Kepada Masyarakat journal of Basic Medical Veterinary Bima Abdi: Jurnal Pengabdian Masyarakat Berkala Penelitian Hayati Indonesian Andrology and Biomedical Journal Jurnal Kedokteran Hewan Smart Dedication: Jurnal Pengabdian Masyarakat

Suherni Susilowati

Departemen Reproduksi Veteriner, Fakultas Kedokteran Hewan Universitas Airlangga Kampus C Unair, Jl. Mulyorejo, Surabaya 60115

Author-ID : 3693314

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Transportation Veterinary Other

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Red fruit oil increases trophoblast cells and decreases caspase-9 expression in placenta of lead exposed mice Sumarsono, Portia; Widjiati, Widjiati; Susilowati, Suherni
Universa Medicina Vol 35, No 2 (2016)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2016.v35.110-118

BACKGROUNDLead is able to pass through the placental barrier and interfere with fetal development. Red fruit has high antioxidant activity, due to carotenoids (pro-vitamin A), tocopherols (vitamin E) and unsaturated fatty acids. This study aims to examine the effect of red fruit oil towards placenta in pregnant mice before lead exposure.METHODSThis was a laboratory experimental post test only study, using 20 pregnant mice (Mus musculus) that were randomized into five groups. The negative control group was not exposed to lead; intervention group 0 (P0) was exposed to lead at 0.011 mg/20 g BW/day on days 6-15 of gestation. The intervention groups P1-P3 were given red fruit orally at 0.3 mL/20 g BW/day, 0.8 mL/20 g BW/day, 0.9 mL/20 g BW/day, respectively, before lead exposure. Normal trophoblast cell count and caspase-9 expression of trophoblasts were calculated. One-way ANOVA, Kruskal-Wallis and Mann Whitney tests were used to analyze the data.RESULTSThe intervention groups P2 and P3 given red fruit at 0.8 mL/20 g BW/day and 0.9 mL/20 g BW/day showed a significant increase in normal trophoblast cell count of 88.2% (p=0.024) and a decrease in caspase-9 expression of 70.8% compared with P0 to which was not given red fruit (p=0.020). CONCLUSIONRed fruit increased normal trophoblast cell count by 88.2% and decreased caspase-9 expression by 70.8% in pregnant mice before lead exposure. It should be noted that red fuit may prevent oxidative damage to cells.

EKSPRESI SPERMATOGENESIS TIKUS PUTIH SETELAH INDUKSI DENGAN PROTEIN INSULIN LIKE GROWTH FACTOR–I COMPLEX PLASMA SEMINALIS KAMBING Suherni Susilowati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/294

The objective of this research was to analyzed the expression of spermatogenesis on white rat after inducted by Insulin Like Growth Factor – I (IGF-I) Complex protein from goat seminal plasm. This research consisted of two phase. The fi rst phase was explorative laboratory experiment concerned with identification, isolation and spesifi cation IGF-I Complex by using gel Native Polyacrylamid gel electroforesis, electroelution and Western Blot. Result of gel Native PAGE indicated that of goat seminal plasm with Comassie Blue Stain consisted of seven bands. Result of Western Blot indicated that molecule of IGF-I Complex of goat seminal plasm to bound specific with anti IGF-I Complex at protein ribbon. The second phase concerned with expretion of spermatogenesis on white rat after induction with isolat of IGF-I Complex protein. Result of the expression spermatogenesis on white rat after induction IGF-I Complex to show the signifi cant different between three groups (p < 0.05). From result above can be concluded that IGF-I Complex protein can increase the total of spermatocyte I and spermatocyte II.

BIOAKTIVITAS INSULIN LIKE GROWTH FACTOR–I COMPLEX PLASMA SEMINALIS KAMBING TERHADAP KUALITAS SPERMATOZOA HASIL SENTRIFUGASI Suherni Susilowati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/349

Series of exploratived laboratory experiment have been conducted to analysed the bioactifity of Insulin Like Growth Factor – I (IGF-I) Complex protein of goat seminal plasm on the membran plasm and acrosom cap of centrifugation sperm. First experiment was conducted to identificatify and isolate IGF –I Complex of goat seminal plasm by using Native - Polyacrylamide gel electroforesis. The result of analysis of Native – PAGE indicated that IGF-I Complex have molecular weight of 150 kDa. Second experiment was conducted to role of IGF-I Complex protein on the membrane plasm and acrosom cap of centrifugation sperm. Semen was collected by using artificial vagina from eight mature goat. Immediately after initial evaluation, semen was centrifugated with Bracket and Oliphant’s (BO) for 5 minutes 1800 rpm. And then divided two groups. Each group was supplemented with 3 × 106 of sperm concentration and then into group I was supplemented with BO medium and group two was supplemented with IGF-I Complex protein. The result of this experiment indicated that percentages of intact plasm membrane and intact acrosom cap were significantly (p < 0.05), between BO medium and IGF-I protein. In conclusion, IGF- I Complex protein is the optimal in improving plasm membran and acrosom cap of centrifugation sperm

Investation of Ectoparasites In Chickens In Village of Kramat District of Bangkalan Regency of Bangkalan Fania Selfiannisa; Suherni Susilowati; Poedji Hastutiek; Lucia Tri Suwanti; Kusnoto Kusnoto; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

A study on the infestation of ectoparasites in chicken’s raised in Village of Kramat, District of Bangkalan, Regency of Bangkalan was conducted between July to October 2017. A total of 60 chickens were examined based on their sex and region of the chicken’s body. Identification was carried out on ectoparasites found identified using permanent mounting methods and scraping. The types of ectoparasites were M. gallinae 77%, L. caponis 52%, M. stramineus 15%, R. sanguineus 3%, G. gallinae 2%, G. gigas 2%, and D. gallinae 2%. Data were analyzed using Chi Square and Correspondence. The results of infestation was higher in males than females, although the difference was not statistically significant (P>0.05). Results of analysis of the pattern of investation of ectoparasites in the region that includes in males and females body are M. gallinae in the region of the back. L. caponis in the region of the wings. M. stramineus. R. sanguineus in the region of the head-neck, G. gigas in the region of belly, G. gallinae in the region of the leg just found in the males body. D. gallinae in the region of the leg just found in the females body.

EFEK LAMA SENTRIFUGASI SEMEN DOMBA TERHADAP PERSENTASE KAPASITASI DAN REAKSI AKROSOM SPERMATOZOA Suherni Susilowati; Dani Hesti Savitri; Nusdianto N
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui efek sentrifugasi semen domba terhadap persentase kapasitasi dan reaksi akrosom spermatozoa. Penelitian ini terdiri atas 3 kelompok semen domba yaitu kelompok kontrol (P0) semen tanpa sentrifugasi, P1 semen disentrifugasi selama 5 menit dengan kecepatan 1800 rpm, dan P2 semen disentrifugasi selama 10 menit dengan kecepatan 1800 rpm. Hasil penelitian menunjukkan bahwa pada kelompok P2 kapasitasi tidak berbeda nyata dengan kelompok P1, tetapi pada kelompok P0 efek kapasitasi dan reaksi akrosom paling rendah. Reaksi akrosom spermatozoa pada kelompok P2 menunjukkan hasil yang paling tinggi. Lama sentrifugasi semen domba ekor gemuk dengan kecepatan sentrifugasi 1800 rpm selama 10 menit dapat meningkatkan persentase kapasitasi dan reaksi akrosom spermatozoa.

PENAMBAHAN PROTEIN INSULIN LIKE GROWTH FACTOR-I COMPLEX DALAM PENGENCER PEMBEKUAN SEMEN TERHADAP KUALITAS SPERMATOZOA KAMBING PADA WAKTU EKUILIBRASI Suherni Susilowati; Tatik Hernawati
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengisolasi protein Insulin Like Growth Factor-I (IGF-I) complex untuk meningkatkan kualitas semen beku kambing pada waktu ekuilibrasi setelah penambahan protein IGF-I complex. Penelitian ini terdiri atas 2 tahap yaitu isolasi protein IGF-I complex dari plasma seminal kambing dan aplikasi terhadap prosesing pembekuan semen. Pada tahap pertama dilakukan identifikasi IGF-I complex dengan menggunakan gel native polyacrylamide gel electrophoresis dan isolasi IGF-I complex. Pada tahap kedua dilakukan aplikasi penambahan protein IGF-I complex pada prosesing semen beku. Semen dikoleksi dengan menggunakan vagina buatan dan kemudian disentrifus selama 5 menit dengan kecepatan 1800 rpm. Kemudian semen dibagi menjadi tiga kelompok. Pada kelompok I, II, dan III ditambahkan protein IGF-I complex masing-masing 0, 12, dan 618 ng/ 3X10 sperma. Selanjutnya dilakukan ekuilibrasi selama 1 jam dan dilanjutkan dengan evaluasi motilitas, viabilitas, dan membran sperma. Hasil penelitian menunjukkan perbedaaan motilitas, viabilitas, dan membran sperma yang signifikan (P0,05) di antara tiga kelompok perlakuan. Dapat disimpulkan bahwa penambahan protein IGF-I complex dapat meningkatkan kualitas semen beku kambing pada fase ekuilibrasi.

Identification of Digestive Tract Endoparasites of Laying Hens in Suruhwadang Village, Kademangan District, Blitar Regency Mega, Toafan Satrio; Susilowati, Suherni; Hastutiek, Poedji; Kusnoto, Kusnoto; Sunarso, Agus; Witaningrum, Adiana Mutamsari
Journal of Parasite Science Vol. 8 No. 1 (2024): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v8i1.52373

This study aims to determine the species, prevalence, and infection degree of endoparasite in the digestive tract of laying hens in Suruhwadang Village, Kademangan District, Blitar Regency. Ninety-six fecal samples were taken from four different farms. Twelve samples of chicken feces aged 20-50 weeks and twelve samples of chicken feces > 50 weeks were taken from each farm. The examinations taken in this study are fecal examinations using native, sediment, and floating methods. The results of identification of digestive tract endoparasites found consisting of Ascaridia galli (68.75%), Heterakis gallinarum (53.12%), Strongyloides avium (5.21%), Trichostrongylus tenuis (6.25%), Eimeria maxima (51,04%), and Eimeria acervulina (3,12%). The highest average degree of worm egg infection was found in layers >50 weeks of age of 373.96 ± 450.41 while the highest average degree of infection of protozoa was found in layers aged >50 weeks of 296,87 ± 600,92. The results of the Chi-Square test showed that there was no significant effect of the difference in the age of laying hens in the layer phase on the prevalence of digestive tract endoparasites, both worm egg and protozoan infections (P>0.05). The results of the Mann-Whitney test showed that there was a significant effect of the difference in the age of the laying hen in the layer phase on the degree of worm egg infection (P<0.05), but the difference in age of the laying hen in the layer phase did not significantly affect the degree of protozoa infection (P>0,05).

Motilitas dan Viabilitas Spermatozoa Sapi Rambon di Desa Kemiren Banyuwangi Prastika, Zeni; Susilowati, Suherni; Agustono, Bodhi; Safitri, Erma; Prastiya, Ragil Angga
Jurnal Medik Veteriner Vol. 1 No. 2 (2018): April
Publisher : Universitas Airlangga

Sapi Rambon merupakan salah satu sapi lokal Indonesia. Tahun 2017 populasi sapi Rambon mengalami penurunan. Salah satu upaya pelestarian sapi lokal adalah melalui peningkatan populasi sapi dan inseminasi buatan. Faktor penentu keberhasilan perkawinan salah satunya adalah libido tinggi dan kualitas semen. Motilitas spermatozoa dan viabilitas spermatozoa adalah salah satu indikator fertilitas spermatozoa dan kualitas semen. Penelitain ini adalah penelitian deskriptif. Penelitian ini bertujuan untuk mengetahui motilitas dan viabilitas semen segar sapi Rambon di Desa Kemiren Kecamatan Glagah Kabupaten Banyuwangi. Semen pada penelitian ini di tampung menggunakan vagina buatan dari 6 ekor sapi Rambon jantan. Pemeriksaan motilitas spermatozoa dan viabilitas spermatozoa dilakukan di klinik Linawati Banyuwangi. Hasil pada penelitian ini disajikan dalam bentuk tabel. Parameter penelitian ini adalah motilitas dan viabilitas spermatozoa sapi Rambon. Hasil pemeriksaan motilitas spermatozoa sapi Rambon 83,3±4,1% dan viabilitas spermatozoa sapi Rambon 86,7±2,3%. Kesimpulan dari penelitian menunjukan bahwa motilitas dan viabilitas spermatozoa sapi Rambon baik dan memenuhi syarat semen beku untuk inseminasi buatan.

Pengaruh Ekstrak Kedelai (Glycine max) Terhadap Proliferasi Lapisan Endometrium Mencit (Mus musculus) Salahuddin, Muhammad Soleh; Safitri, Erma; Yunita, Maya Nurwartanti; Susilowati, Suherni; Hamid, Iwan Sahrial; Yudhana, Aditya
Jurnal Medik Veteriner Vol. 2 No. 1 (2019): April
Publisher : Universitas Airlangga

Tujuan penelitian ini adalah mengetahui pengaruh ekstrak kedelai untuk meningkatan proliferasi lapisan endometrium dalam proses perkembangan organ uterus pada mencit (Mus musculus) betina. Kelompok kontrol (K) tidak diberi perlukaan. Kelompok P1 diberi ekstrak kedelai dengan dosis 12,5mg/kgBB. Kelompok P2 diberi ekstrak kedelai dengan dosis 25mg/kgBB. Kelompok P3 diberi ekstrak kedelai dengan dosis 37,5mg/kgBB. Kelompok P4 diberi ekstrak kedelai dengan dosis 50mg/kgBB. Perlakuan dilakukan setiap hari sekali selama empat belas hari. Hasil uji One Way Anova menunjukkan perbedaan yang signifikan (p<0,05) dan dilanjutkan dengan uji Duncan's Multiple Range Test (DMRT). Ketebalan endometrium kelompok P2, P3, dan P4 berbeda secara nyata (p<0,05), dengan kelompok kontrol (K), dan kelompok kontrol (K) tidak berbeda nyata dengan kelompok P1 (p>0,05), serta kelompok P1 tidak berbeda nyata dengan kelompok P2 (p>0,05). Kandungan senyawa fitoestrogen yaitu isoflavon berupa daidzein dan genistein bekerjasama dalam mengoptimalkan proliferasi lapisan endometrium pada uterus mencit (Mus musculus) betina. Daidzein bekerja sebagai senyawa estrogenik untuk berikatan dengan reseptor estrogen pada uterus dan genistein bekerja sebagai penghambat pertumbuhan lapisan endometrium yang berlebih pada jumlah tertentu. Kesimpulan dari penelitian ini adalah ekstrak kedelai (Glycine max) efektif untuk peningkatan proliferasi lapisan endometrium pada uterus mencit betina.

Difference Time Effect of Equilibration Before Freezing on The Quality of Spermatozoa Sapera Goats using Egg Yellow Tris Prayogo, Danu; Susilowati, Suherni; Prastiya, Ragil Angga; Safitri, Erma; Agustono, Bodhi
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.188-195

This study aimed to examine the effect of time differences on motility, viability, abnormality, and intact plasma membrane of spermatozoa of Sapera goats before freezing. This study used a Completely Randomized Design (CRD) with three settings, namely the equilibration time P1 (1 h), P2 (3 h), and P3 (4 h), each of six replications. The results of motility using One Way Anova statistical analysis through the Duncan Test were (P1) 71.50 ± 3.94, (P2) 66.00 ± 2.60, and (P3) 60.33 ± 2.42. The results of viability was (P1) 83.00 ± 6.16, (P2) 75.17 ± 4.70, and (P3) 69.00 ± 3.40. Abnormal results was (P1) 2.50 ± 0.55, (P2) 3.33 ± 1.18, and (P3) 6.50 ± 2.88. While the results of intact plasma membranes were (P1) 79.50 ± 8.50, (P2) 72.67 ± 5.28, and (P3) 65.67 ± 3.78. The conclusion of the study showed significant significant results (p<0,05) between the equilibration time and spermatozoa quality of Sapera goats before freezing. The highest presentation of motility, viability and overall plasma membrane was at an equilibration time of 1 h, while the lowest abnormality presentation was at equilibration time of 1 h.

Co-Authors A Ashiila Tsabita Abdul Samik, Abdul Abuzahra, Mutasem Adiana Mutamsari Witaningrum Aditya Irawan Agustono, Bodhi Akintunde, Adeyinka Oye Aksono HP., Eduardus Bimo Al Arif, M Anam Al arif, Mohammad Anam Ali Agus Ali Agus Anas Bad’rosana Prawindi Anugrah, Alvyan Lantang Ardina Sahra Miranda Arini, Mirza Asafaizh Aris Puji Nugroho Ayuti, Siti Rani Azura, Sarah Brahmantya, Chandra Budi Utomo BUDI UTOMO Candika, Yossy Imam Cesaria, Mayrena Ayu Chusniati, Sri Damayanti L, Tita Damayanti, Tita Dani Hesti Savitri Dani Hesti Savitri, Dani Hesti Dewi, Aprilia Kurnia Dian Ayu Permatasari Diba, Lucky DWI WIJAYANTI Eka Pramyrtha Hestianah Emy Koestanti Sabdoningrum Erma Safitri ERMA SAFITRI Estoepangestie, A.T.Soelih Fajar Ramadhan Subiyantoro Fania Selfiannisa Hana Eliyani Hani Plumeriastuti Hardijanto . Hardijanto, Hardijanto Hariadi, Mas'ud Hendarti, Gracia Angelina Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidajati, Nove Hidanah, Sri IMAM MUSTOFA Immelda, Kicky Hanis Indah Norma Triana, Indah Norma Indira, Sitti Az-Zahra iqbal dwi warsito Ira Sari Yudaniayanti Irvanto, Rudi Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Khairullah, Aswin Rafif Khoirunnisa, Itrah Koestanti S, Emy Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Mafruchati, Maslichah Mas'ud Hariadi Mega, Toafan Satrio Meles, Dewa Ketut Mirni Lamid Moh. Sofiul Anam Muhammad Ikmal Robih Mustofa Helmi Effendi Nawangwulan, Tantri Nove Hidajati Nusdianto N Nusdianto N, Nusdianto Nusdianto Triakoso Pitaloka, Dinda Galuh Poedji Hastutiek Portia Sumarsono, Portia Prastika, Zeni Prastiya, Ragil Angga Prastyaningtyas, Kukuh Pratama Putra Banusiandana Pratama, Abrian Panggalih Indra Prayogo, Danu Pudji Srianto Purnawan, Ahmad Budi Puspita, Satya Alysa Cahya Putri, Cindy Ercha Aulia Qodri, Winda Nurul Lailatil Rahmatullah, Aldin Akbar Rahmi Sugihartuti, Rahmi Ratnaningtyas, Nurrohmah Retno Sri Wahjuni Retta, Ayun Tria Marga Rimayanti Rimayanti Riza Zainuddin Ahmad Rochmah Kurnijasanti ROHMAH, LAILATUL Rosyada, Zulfi Nur Amrina Sabdoningrum, Emy Koestanti Sabdoningrum, Emy Koestanty Salahuddin, Muhammad Soleh Samuel Inioluwa Akeju Saputra, Rakan Mahiid Saputro, Amung Logam Shinta Putri Quraini Soeharsono Soeharsono Soepranianondo, Koesnoto Sri Mulyati Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Sunarso, Agus Suprihati , Endang Supriyadi Supriyadi Supriyadi Suryaningsih, Hastini Suzanita Utama Swari, Wahyu Retno Syaiful Rizal Tatik Hernawati Tintin Sukartini Sukartini, Tintin Sukartini Tita Damayanti Lestari Tjuk Imam Restiadi Tri Nurhajati, Tri Tri Wahyu Suprayogi Trilas Sardjito Tyasmingsih, Wiwiek Utama, Dirga Januar Surya Viski Fitri Hendrawan Vladika, Maulinda Agchirilia Warsito, Sunaryo Hadi Widjiati Widjiati Widjiati Widjiati, Widjiati Widya Paramita Widya Paramita Lokapirnasari Widya Paramita Lokapirnasari Wurlina, W Yeni Dhamayanti Yudhana, Aditya Yunita, Maya Nurwartanti

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