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Irwan Effendi
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INDONESIA
Jurnal Natur Indonesia
Published by Universitas Riau
ISSN : 14109379     EISSN : 25030345     DOI : https://doi.org/10.31258/jnat
Core Subject : Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry
Jurnal Natur Indonesia published since 1998, is a science journal that presents articles on research results, thoughts and views from researchers and experts in the field of biosciences (basic science), including biology, physics, chemistry and mathematics. In the field of biosciences (basic science); Biology, Physics, Chemistry, Mathematics.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 15 Documents
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Search results for , issue "Vol 12, No 1 (2009)" : 15 Documents clear
Identifikasi Genetik Mutan Talinum paniculatum JACQ. (GAERTN.) Berdasarkan Marka RAPD Poerba, Yuyu Suryasari
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.61 KB) | DOI: 10.31258/jnat.12.1.44-48

Abstract

Javasom Talinum paniculatum Jacq. (Gaertn.) is one of popular vegetable plants with potential medicinal properties.The plant root is often used as a substitute for ginseng (Panax ginseng L.), and the leaves are used as a vegetableand as a substitute for purslane (Portulaca olaraeae L.). An effort to improve genetic quality of the plant was madethrough induced mutation with ethyl methane sulphonate (EMS). A variety of EMS dosages (0, 0.3%, 0.6%, 0.9%,1.2%, 1.5% dan 1.8%) were applied to javasom seeds to induce mutation for 24 hours at room temperature. Selectedfive putative mutant genotypes of the third generation after mutagen treatment (M3) was analyzed using Ramdomamplified polymorphic DNA (RAD) marker. Twenty RAPD primers generated 185 scorable bands with 78.92% ofthem were polymorphic. Size of the bands varied from 200bp to 2.5kbp. Clustering analysis was performed basedon RAPD profiles using the UPGMA method. The range of genetic distance among individual genotypes was fromfrom 0.31 to 0.58, while genetic variance was 0.29 + 0.17. The five genotypes were proof to be solid mutants.
Sintesis dan Aplikasi Karboksimetil Kitosan sebagai Inhibitor Korosi pada Baja Karbon dalam Air Erna, Maria; Emriadi, Emriadi; Alif, Admin; Arief, Syukri; Noordin, Mohd Jain
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.132 KB) | DOI: 10.31258/jnat.12.1.87-92

Abstract

Carboxymethyl chitosan (CMC) was synthesized with different methods by reacting chitosan with monochloroaceticacid in the presence of sodium hydroxide under variation conditions. The above samples were characterized byFourier transform infrared spectroscopy (FTIR) and soluble in water in range of pH. The CMc were soluble in watera wide range of pH and applied as corrosion inhibitor for steel in water. The inhibiting influence of CMC was studiedby potentiodynamic polarization method. It was found thad corrosion rate was dependent on water pH and CMCconcentration. The results show that optimum the inhibition efficiency at pH 5 and 1 ppm concentration CMC, i.e.,77%. The adsorption of used compound on the steel surface obeys modified Langmuir isotherm. Polarizationmeasurement show that the CMC acts essentially as a anodic-type inhibitor.
Biomassa Kerang Anadara granosa pada Perairan Pantai Kabupaten Indragiri Hilir Nasution, Syafruddin
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.34 KB) | DOI: 10.31258/jnat.12.1.61-66

Abstract

The present study was carried out to investigate the biomass of cockle (Anadara granosa) from coastal waters ofSouth Indragiri District, Riau Province. The biomass was investigated by quadrad sampling method in the intertidalarea along the Concong beach, where fishing of cockles mainly occured. The first station was near by the Concongestuary, while the second station was about 3 miles away from the fisrt one. Samples were collected from threedifferent portions of the intertidal zone that of High Intertidal Mid-Intertidal, and Low Intertidal. The result showedthat the highest biomass was found at the lower partion of the intertidal zone. Soft tissue of A. granosa was about24.8% of total wet-weight and 7.2 % of dry-weight. While material organic was about 85 % of soft tissue dry-weight.Water qualities of the sampling station showed that the overall water qualities were still in normal condition.
Cyclodextrin Formation Initiated by Enzyme Debranching Reaction On Amylopectin Branch Chain Of Tapioka Laga, Amran
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.093 KB) | DOI: 10.31258/jnat.12.1.15-20

Abstract

Degradation of starch by the glucosyltransferase enzyme (CGTase) to produce the primary product of chainsplitting undergoes an intramolecular reaction without the participation of water molecule. From this process, α-1,4-Linked cyclic product, known as cyclodextrins, are formed. The aim of the research was to cut amylopectinbranch in order to produce one straight chain, to optimize cyclic reaction formation cyclodextrin by CGTase. Theresearch was devided into 3 stages; (1) debranching enzyme concentration estimation (5,10,15,20, and 25 unit/gram) and the length of otimum reaction to produce straight chain for 5 hours which sample was taken each hour,(2) reaction length time estimation to form cyclodextrin in order to use debranching products (straight chain) assubstrates, the reaction length for 360 minutes and sample taken each for 30 minutes, and (3) the best substrateconcentration for straight chain (20-40% w/v) to produce cyclodextrin. The result showed that enzyme concentrationtreatment and optimal length reaction will produce straight chain with enzyme concentration of 14 units/gram for3 hours and straight chain product of 20 units/gram for 1 hour with straight chain product of 83.5%. The optimumlength of reaction for cyclodextrin formation from amylose produced from the de-branching process was 240minutes. The amount of cyclodextrin produced was 143.45 g/L with conversion value of 47.81% at 30% (w/v)substrate concentration. Highest yield of cyclodextrin (154,28 g/L) and conversion value of 44.08% was obtained at35% (w/v) substrate concentration
Senyawa Antibakteri Escherichia coli ATCC 35218 dan Staphylococcus aureus ATCC 25923 dari Kapang Endofit Taman Nasional Gunung Halimun Melliawati, Ruth; Harni, Harni
Jurnal Natur Indonesia Vol 12, No 1 (2009)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.739 KB) | DOI: 10.31258/jnat.12.1.21-27

Abstract

Endophytic fungus is a microorganism which live in the interstitial spaces healthy tissues of the host plant, andhas capability to produce secondary metabolite such as micotoxin, antibiotic and antiviral. This research wasaimed to find out the isolates of endophytic fungus produce antibacterial compounds to inhibit Escerichia coliATCC 35218 and Staphylococcus aureus ATCC 25923, and to investigate the Retention time (Rt) of the antibacterialcompounds produced by endophytic fungus with High Performance Liquid Chromatography (HPLC) methods.Diffusion Agar Plate Method was used to examine the antibacterial compounds on Escerichia coli ATCC 35218 andStaphylococcus aureus ATCC 25923. While the antibacterial compounds were examined with Thin LayerChromatography (TLC) and High Performance Liquid Chromatography (HPLC) methods, and the result werecompared with Chloramphenicol and Ampicillin antibiotic standart. Two isolates of endophytic fungus namelyHl.46F.211 and HL.57F.258 were inhibited the growth Escherichia coli ATCC 35218 and three isolates namelyHL.48F217, HL.53F.243 and HL.57F.258 showed antagonistic action against Staphylococcus aureus ATCC 25923.The results of TLC and HPLC analysing method show that antibacterial compounds produced by endophytic fungusHL.46F.211 had Rt (Retention Time) rate similar with antibiotic Chloramphenicol at 2,796 (at water fraction) and Rtantibiotic Amphycillin at 2,662 (at Chloroform fraction). While antibacterial compounds produced by endophyticfungus HL.57F.258 had Rt rate similar with antibiotic Amphycillin at 2,650 (at Chloroform fraction).

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