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Elvi Rusmiyanto PW
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Protobiont: Jurnal Elektronik Biologi
Published by Universitas Tanjungpura
ISSN : -     EISSN : 23387874     DOI : http://dx.doi.org/10.26418/protobiont.
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Jurnal Protobiont merupakan jurnal peer-reviewed dan open access journal yang mempublikasikan hasil-hasil penelitian biologi tropis yang berkaitan dengan bidang: 1. botani, 2. zoologi, 3. ekologi, 4. mikrobiologi, 5. genetika, 6. ilmu lingkungan, dan 7. biologi konservasi. Jurnal Protobiont terbit setahun 3 kali, yaotu bulan Maret, Juli, dan November. Jurnal Protobiont diterbitkan dan dikelola oleh Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 12 Documents
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Search results for , issue "Vol 3, No 3 (2014)" : 12 Documents clear
Kapang pada tingkat kematangan gambut yang berbeda di kawasan Hutan Lindung Gunung Ambawang Kabupaten Kubu Raya Siti Khotimah, Efa Rosita, Riza Linda,
Jurnal Protobiont Vol 3, No 3 (2014)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v3i3.7122

Abstract

Peat is formed from organic materials derived from the remains of dead plants and accumulated over thousands of years. Dead plants will undergo decomposition quickly by molds and bacteria. This research aims to identify the genus of molds at different levels of maturity of peat in the area of the Protected Gunung Ambawang Forest in Kubu Raya District. Peat sampling used the stratified random sampling method based on the degree of maturity of the peat soil i.e. fibric peat, hemic peat, and sapric peat. The isolation of molds employed the method of dilution plate with dilution 10-3 at the medium of PDA (Potato Dextrose Agar) and CYA (Czapeck Yeast Agar). The result of research on fibric peat showed that six pure isolates of mold were found i.e. Aspergillus niger, Fusarium sp.1, Paecilomyces sp.2, Penicillium variabile, Phialophora sp., and Verticillium sp. On hemic peat 10 pure isolates were found: A. niger, Fusarium sp.2, Mortierella sp., Paecilomyces sp.1, Paecilomyces sp.3, Penicillium sp.3, Penicillium sp.4, Penicillium sp.5, Rhizopus sp., and T. harzianum. On sapric peat 12 pure isolates were found: Fusarium sp.3, Paecilomyces sp.1, Paecilomyces sp.4, Penicillium sp.1, Penicillium sp.2, Penicillium sp.3, Penicillium sp.6, Penicillium sp.7, Penicillium sp.8, Penicillium sp.9, Pythium sp., and Trichoderma sp.
Karakter morfologi bakteri dari daun sehat dan bergejala sakit Lidah Buaya (Aloe vera var. barbadensis) Ahmad Mulyadi, Yoas Leo G.M.T, Siti Khotimah,
Jurnal Protobiont Vol 3, No 3 (2014)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v3i3.7549

Abstract

Plant diseases caused by bacteria will decrease the production of Aloe vera var. barbadensis. Bacteria that infect the leaves of Aloe vera can be recognized by symptoms on plants that the leaves become soft rot and dry rot. This study aims to determine the density of bacterial colonies, colony morphology and the morphology of bacterial cells of healthy leaves and leaves of symptomatic illness. The method used is the Total Plate Count (TPC). The results showed that most bacteria found in the leaves of symptomatic illness that 7 isolates soft rot and bacterial colony density that is 237 X 10-7cfu/g. Bacteria were recovered from healthy and symptomatic illness leaves are 16 isolates, that showed characteristic colony circumference shape, curved, long, flat elevation, convex, flat, flat edges, uneven and curved, while the morphology of bacterial cells are coccus, bacillus and spirilum.
Pemanfaatan Tumbuhan pada Upacara Adat Tumpang Negeri Suku Melayu di Keraton Ismahayana Landak Irwan Lovadi, Uswatun Hasanah, Riza Linda,
Jurnal Protobiont Vol 3, No 3 (2014)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v3i3.7355

Abstract

Traditional Ceremony Tumpang Negeri is a tribal ritual of Malayan people that utilizes plants to prevent misfortune so that people of Landak Regency might be averted from menaces and that Landak egency might be a prosfer, safe, and peacefull land. The aim of this research was to investigate the plant spesies and their utilization in Tumpang Negeri. This research was conducted from January to March 2014. The method of respondence selection that used in this research was snowball. The result of this research shows that there are 23 plant species which belong to 12 families, have been used in Tumpang Negeri Ceremony. Most of the plants used belong 3 families which are Poaceae (5 species), Arecaceae (4 species), and Zingiberaceae (4 species). The plants are used as materials for making traditional snacks, rafts, and tumpang for offerings. Part of the plants that mostly used are the fruits (45 %), leaves (32 %), roots (18 %), and stems (5 %). The plants were harvested mostly from the forest (35 %), the botanical gardens (31 %), the home garden (30 %), and the fields. Every plant species used in Traditional ceremony tumpang Negeri has specifik meanings.
Aspek mikrobiologis susu sapi murni dengan penambahan sari rimpang Bangle (Zingiber cassumunar Roxb) Masnur Turnip, Rosalia Juniati Nahak, Siti Khotimah,
Jurnal Protobiont Vol 3, No 3 (2014)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v3i3.7556

Abstract

Milk is food which contains nutrients such as proteins, fats, carbohydrates, minerals, and vitamins. Milk is also a growing medium for microorganisms. This study aims to determine the quality of pure cow's milk after bangle rhizome extract (Zingiber cassumunar Roxb) being added to it. The study was conducted from January to March 2014. The parameters observed in this study is TPC (Total Plate Count) by using the pour plate method, the calculation of the amount of coliform by using MPN method, the computation of time reduction by using reductase method and organoleptic test by using taste panel method. Based on the research findings, it was revealed that the extract of rhizome bangle added to the milk at a concentration of 2,4% can reduce the total number of bacteria from 2,47 × 105 to 0,73 × 105, reduce the amount of coliform bacteria from 210 CFU / ml to 7 CFU / ml and prolong the time reduction up to 700 minutes (11.6 hours) and make the results of organoleptic test accepted by the panelists.
Perbaikan kualitas lindi TPA Batu Layang menggunakan arang batok kelapa, arang kulit durian dan pasir Irwan Lovadi, Ika Nurmaja, Tri Rima Setyawati,
Jurnal Protobiont Vol 3, No 3 (2014)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v3i3.7550

Abstract

The alternative filter media  that  used to process the leachate water is charcoal and sand. This research aims to determine the effectiveness of using coconut shell charcoal, durian rind charcoal and sand in improving the quality of leachate in terms of the Total Suspended Solid (TSS), Total Disolve Solid (TDS), pH, Chemical Oxygen Demand (COD) parameters and the number of bactery colonies. This research was conducted for three months from December 2013 to February 2014. The research was designed by using the Randomized Block Design (RBD) with each coconut shell charcoal and sand has 10 cm thickness (treatment K), each durian rind charcoal and sand has 10 cm thickness (treatment D), each coconut shell charcoal, durian rind charcoal, and sand has 10 cm thickness (treatment KD), and sand has 10 cm thickness (control). The value of TSS, TDS, pH, COD and the number of bactery colonies before treatment were 430 mg/L, 1142 mg/L, 5.73, 92.27 mg/L and 407.7 x 105 CFU/ml. The most effective treatment was each coconut shell charcoal, durian rind charcoal and sand has 10 cm thickness which can reduce TSS 46.91%, TDS 47.00%, COD 47.03%, and the number of bacteria colonies 40.96% and increased the pH 7.33.
Pertumbuhan stek batang kantong semar (Nepenthes bicalcarata Hooker) dengan penambahan Indole Butyric Acid (IBA) Riza Linda, Sutria Ningsih, Mukarlina,
Jurnal Protobiont Vol 3, No 3 (2014)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v3i3.7115

Abstract

Nepenthes bicalcarata commonly grows in the edge of swampy mangrove forest and heath forest which are poor in nutrients and have a relatively high humidity. The conservation effort on  N. bicalcarata can be done through plant multiplication by stem cuttings. This study aims to determine the effect of  indole butyric acid (IBA) growth regulator addition on the growth of N. bicalcarata stem cuttings and to determine the concentration of  IBA growth regulator  that can give the best growth on  N. Bicalcarata stem cuttings. The study was conducted in November 2013 to February 2014. Based on  ANOVA analysis, the addition of IBA on N. bicalcarata stem cuttings significantly affected the height of the plant and the number of roots. The best IBA concentration given on N. bicalcarata stem cuttings was at 15 ppm, resulting in 19,50 cm of  average plant  heights and  27,25 of average roots number. The addition of IBA did not significantly affect the number of  leaves and initial budding period.
Karakteristik fisika-kimia pengomposan limbah kulit durian (Durio zibethinus L.) menggunakan cairan rumen sapi Aditya, Anang
Jurnal Protobiont Vol 3, No 3 (2014)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v3i3.7568

Abstract

Durian peel is identified to take a long period of time to decay or degradation, thus it requires a pretreatment to simplify the process of hydrolysis. This study aims to determine the condition of chemical physic composting using cow’s rumen liquor. The study used several concentrations and combinations of treatment. Parameters observed in this study include substrate temperature, moisture content, pH, Total Organic C, Total Organic N, and C/N ratio as well. The results obtained i.e. the average temperature of the substrate is 29,090C, the average moisture content of the substrate is 87.55%, and the average pH of the substrate is 7.82. The percentage change of the Organic C is 0.52% for the treatment of 30%. The percentage change of the Organic N is 36.76%. The Percentage change of C/N ratio is 41.36%. These results indicate a distinctive percentage value from various treatments with the highest concentration at 30% of cow’s rumen liquor on Organic C, Organic N as the control, and C/N ratio as well.
Anatomi Daun dan Ranting Citrus nobilis L. var. microcarpa yang Terserang Citrus Vein Phloem Degeneration Riza Linda, Heni Susanti, Mukarlina,
Jurnal Protobiont Vol 3, No 3 (2014)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v3i3.7552

Abstract

Citrus Vein Phloem Degeneration (CVPD) is one of the diseases that cause a decrease in the production of the Citrus nobilis L. var. microcarpa. The CVPD disease is caused by the Liberibacter asiaticus bacteria and transmitted through by psyllid (Diaphorina citri). The infected C. nobilis L. var. microcarpa indicates morphological changes which can be divided into mild, moderate, and severe symptoms. The main purpose of this research was investigating citrus leaf and twig anatomy which was infected by CVPD. The research was conducted from February 2014  until August 2014. Paraffin method was used to make cross section sample preparation of the citrus leaf and twig. The result on cross section of leaves, petioles, and twig CVPD symptomatic indicated there was cell wall thickening on epidermis. Mesophyll (leaf) and cortexs (petioles and twig) shape changes and there is an acumulation  of starch. The Phloem on leaves, petioles, and twigs indicated changes in cell shape in which it  becomes abnormal then damage (necrosis) occured.
Kondisi hematologi pemulung yang terpapar gas amoniak di tempat pembuangan akhir (TPA) sampah Batu Layang Pontianak Ari Hepi Yanti, Niske Puspita Sari, Tri Rima Setyawati,
Jurnal Protobiont Vol 3, No 3 (2014)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v3i3.7492

Abstract

Ammonia is naturally formed from the breakdown of proteins in the decomposition of organic waste. The aim of this research is to understand the level of ammonia in ambient air and in the blood of the gerbage collectors at TPA Batu Layang Pontianak, as well as their hematologic condition after the gas exposed. This research had been done from October to November 2013. The ammonia level measurement was done by using Indofenol method of 0.0223 – 0.0817 ppm. Both male and female gerbage collectors, who had been working for more than 5 years, had the highest ammonia in blood levels of 0.719 mg/l and 0.705 mg/l respectively. The result of the regression analysis showed that the gerbage collectors, who had worked for longer period, had the higher ammonia and the lower hemoglobin level in their blood. The ammonia in blood level also caused the defective erythrocytes membrane and the systolic blood pressure, so that it became unstable. The result also showed that erythrocytes have shape abnormality which is categorised as elliptocytes. A garbage collector, who had the lowest ammonia level (0.058 mg/l) had the damaged erythrocytes percentage of 12.13%. Whereas, the garbage collectors that had the highest ammonia level (0.719 mg/l) had the damaged erythrocytes percentage of 30.09%.
Aktivitas antibakteri ekstrak rimpang Jeringau (Acorus calamus L.) terhadap pertumbuhan Staphylococcus aureus dan Escherichia coli Ari Hepi Yanti, Anisah, Siti Khotimah,
Jurnal Protobiont Vol 3, No 3 (2014)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v3i3.7114

Abstract

Jeringau (Acorus calamus L.) is an herb that is used to treat diarrhea, tapeworm and dysentery. If it is mixed with several other materials, it can help women after they are giving birth. This study was done to evaluate A. calamus’s antibacterial activity to inhibit the growth of Staphylococcus aureus and Escherichia coli. Ethanol and water were used as solvents to extract the Jeringau’s rhizome. The method used to test the antibacterial activity performance was the paper disk diffusion method in 25%, 50%, 75% and 100% v/v (ml/ml). The positive control was cloramphenicol, and the negative control was aquades. Based on the fitochemical test, A. calamus’s extract contained secondary metabolites compounds, such as alkaloid, flavonoid and polifenol. The results showed that there were inhibitions activities from the ethanol and water extract toward both of the tested bacteria. Water extract had smaller inhibition zone compared tto the ethanol. Different types and concentrations of solvents have different ability in inhibiting the growth of S. aureus and E. coli. The 100% concentration of both ethanol and water extract performed the highest activity to inhibit each bacterium growth.

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