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Ivandini Tribidasari A.
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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 7 Documents
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Search results for , issue "Vol. 23, No. 1" : 7 Documents clear
Decolorization of Synthetic Dyes by Ligninolytic Enzymes from Trametes hirsuta D7 Anita, Sita Heris; Sari, Fahriya Puspita; Yanto, Dede Heri Yuli
Makara Journal of Science Vol. 23, No. 1
Publisher : UI Scholars Hub

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Abstract

The ability of ligninolytic enzymes from Trametes hirsuta D7 to decolorize several synthetic dyes was investigated. A crude enzyme powder was produced by fermenting oil palm empty fruit bunch fibers for one month. The dye decolorization by the enzymes occurred at an efficient 0.25 U/mL. The enzymes degraded 100, 200, 300, 400, and 500 ppm Remazol Brilliant Blue R (RBBR) within 7 h by 95.57 ± 0.32%, 93.46 ± 3.09%, 91.84 ± 0.65%, 86.44 ± 0.97%, and 82.14 ± 0.52%, respectively. The enzyme also decolorized anthraquinone (Acid Blue 129), monoazo (Acid Orange 7), diazo (Reactive Black 5), and trimethyl methane (Methyl Violet) dyes within 7 h by 94.59 ± 7.97%, 13.99 ± 0.30%, 7.61 ± 0.01%, and 7.59 ± 0.18%, respectively. Addition of MnSO4, H2O2, and violuric acid enhanced the dye decolorization rate by up to 10-fold. This study shows the potential for application of ligninolytic enzymes from T. hirsuta D7 in the treatment of wastewater effluent of textile industries.
Eco-friendly and Convenient Synthesis of Biologically Active Polysubstituted Dihydro-2-Oxypyrroles Using Manganese (II) Nitrate Tetrahydrate at Ambient Temperatures Mohamadpour, Farzaneh
Makara Journal of Science Vol. 23, No. 1
Publisher : UI Scholars Hub

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Abstract

A convenient, expedient and efficient method for the synthesis of polysubstituted dihydro-2-oxypyrroles is described via the one-pot four-condensation of dialkyl acetylenedicarboxylate, formaldehyde, and amines (aromatic and aliphatic) at ambient temperature in the presence of Manganese (II) Nitrate Tetrahydrate (Mn(NO3)2.4H2O) as a cost effective and inexpensive catalyst. The present methodology provides a simple and eco-safe procedure for the synthesis of polysubstituted dihydro-2-oxypyrroles with some additional advantages, such as good to high yields, short reaction times, avoidance of hazardous or toxic catalysts, simplicity of operating and work-up procedures with no necessity for chromatographic purification steps.
Bioinformatics Toxicity Assessment of RB Protein from Transgenic Potato Resistant to Phytophthora infestans Hadiarto, Toto; Ambarwati, Alberta Dinar
Makara Journal of Science Vol. 23, No. 1
Publisher : UI Scholars Hub

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Abstract

The genetically engineered potato cultivar Katahdin SP951 is resistant to late blight disease, which is caused by Phytophthora infestans. The biosafety and food safety of this cultivar should be assessed prior to its commercialization. Toxicity is one of the parameters tested in the food safety evaluation of transgenics. Toxicity testing includes the bioinformatics analysis of the homology of the RB protein of Katahdin SP951 with known proteins. Therefore, this study aimed to perform the bioinformatics analysis of Katahdin SP951 RB protein against homologous toxin proteins. Bioinformatics analysis was conducted by first translating the RB gene into an amino acid sequence by using the Emboss Transeq program from the EMBL-EBI website. The Shuffle Protein Program was then applied to obtain the randomized amino acid sequences of the RB protein. The Basic Local Alignment Search Tool (BLAST) Protein search program was utilized to identify proteins with high similarity and homology. Moreover, BLAST Suite-2 was used to analyze the homology between two or more sequence alignments. Results showed high homology between the sequences of the RB protein and those of known resistance (R) proteins with an E value of less than 0.22. This result may be attributed to the presence of numerous R proteins in plants. Further analysis indicated that the sequence of the RB protein had extremely nonsignificant homology with sequences of proteins known to be toxic in the Entrez protein database of National Center for Biotechnology Information. Therefore, the RB protein is nontoxic.
Effect of Backbone, Sequence, and Positional Disorders on Electrical Transport in Modified Poly(dA)–Poly(dT) DNA Wire Suhendro, Daniel Kurnia; Yudiarsah, Efta; Saleh, Rosari
Makara Journal of Science Vol. 23, No. 1
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The effect of medium and thermal fluctuations on charge transport in two types of modified poly(dA)–poly(dT) DNA was studied by calculating the transmission probability and current–voltage (I–V) characteristics of a model DNA wire sandwiched between two metal electrodes. Modification was performed by randomly replacing several A–T base pairs with C–G or G–C base pairs along the DNA chain. The medium–DNA interaction was modeled as the backbone onsite energy disorder in the DNA tight-binding Hamiltonian. The helicity of the molecule was considered by incorporating twist-angle-dependent intrastrand hopping amplitude in the model. Thermal fluctuation was modeled by varying the twist angles of each base in the DNA wire. Twist-angle disorder was influenced by temperature and frequency. The I–V results obtained by modeling the backbone disorder effect showed that the current decreased and the threshold voltage generally increased as disorder strength increased to a critical value. The current increased and the threshold voltage decreased as the disorder strength exceeded this critical value. However, certain values of the backbone disorder reduced the threshold voltage before the critical value was reached because the transmission bands shifted toward the Fermi energy. The results of thermal fluctuation modeling indicated that increasing thermal fluctuation (increasing temperature and decreasing frequency) degraded the electrical properties of the DNA modified with C–G base pairs but enhanced those of the DNA modified by G–C base pairs. This trend, however, did not always hold for all frequency values for the latter DNA type.
Role of Indigenous Nitrogen-fixing Bacteria in Promoting Plant Growth on Post Tin Mining Soil Widawati, Sri; Suliasih,
Makara Journal of Science Vol. 23, No. 1
Publisher : UI Scholars Hub

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Abstract

Post tin mining soil is generally marginal with low pH, has poor nutrient content, and is thus unfavorable for plant growth, particularly for Sorghum bicolor, which is a nutrient-demanding plant. Indigenous bacteria are usually used in bioaugmentation to ameliorate environmental degradation due to their ability to adapt well. This research aimed to isolate indigenous nitrogen-fixing bacteria and evaluate its potential for promoting the growth of S. bicolor on post tin mining soil. Nitrogen-fixing bacteria were isolated from post tin mining soil by using specific media and identified by Bergey’s manual. Twenty five isolates were obtained, and eight of them (Azospirillum sp., Azospirillum lipoferum, Azotobacter chroococcum, A. paspalii, and Rhizobium sp.) were identified as nitrogen-fixing bacteria. A greenhouse experiment was conducted using factorial completely randomized design with three replications. The first factors were fertilizers, i.e., NPK; A. lipoferum CBT4 + NPK; A. lipoferum CBT4; and without fertilizer (control). The second factors were soil types, i.e., A (fertile soil from Cibinong), B (soil from Bangka Botanical Garden), C (soil from post tin mines two years after mining), and D (soil from active tin mining). Result showed that Azospirillum lipoferum CBT4 isolated from C (soil from post tin mines two years after mining) exhibited the highest IAA, Ca-P solubilizing ability, and PME-ase activity. This species survived up to a population of 107 CFU/gram soil in the three types of post tin mining soils and could be a potential plant-growth promoting rhizobacteria (PGPR) species for effectively improving the growth of S. bicolor plant on post tin-mining soil.
Determining Soft Layer Thickness Using Ambient Seismic Noise Record Analysis in Kota Baru, South Lampung Ipmawan, Vico Luthfi; Permanasari, Ikah N. P.; Siregar, Rahmat Nawi
Makara Journal of Science Vol. 23, No. 1
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Abstract

The local site effect of an earthquake can be calculated through an analysis of short period ambient noise, known as microtremors. The fundamental frequency and amplification factors can be identified by analyzing microtremors using the Horizontal to Vertical Spectral Ratio (HVSR) method. This information can then be used to determine the thickness of the soft layers of sediment. This study analyzed microtremor recordings made in Kota Baru, South Lampung. The amplification factor range according to the HVSR method was 2.3 to 6.17, and the fundamental frequency range was 0.56 Hz to 1.46 Hz. Some spectrums exhibit two peaks with f0 > f1. We suggest that these locations have two layers with significant impedance contrast, which aligns with the geological conditions. The center of the Kota Baru area, especially the region around T11 and T15, has a thicker soft layer than the outskirts; in the central area, the soft layer is approximately 85 to 102 meters thick. The western part of the analyzed area has a shallower soft layer of about 32 to 46 meters. The analysis indicates that the western part has less amplification because it is shallower than the other parts of the analyzed region.
Decontamination of Ni(II) and Co(II) ions from Aqueous Medium using the Cola Lepidota Pericarp Oladimeji, Adesanmi Segun; Uzoamaka, Ibezim Ezeani Millicent; Chidi, Obi
Makara Journal of Science Vol. 23, No. 1
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Abstract

In this study, the batch adsorption of Ni(II) and Co(II) ions was investigated by using the pericarp of Cola lepidota as the adsorbent. The pulverized sample was modified using sodium hydroxide. In addition, the effects of pH, particle size, contact time, temperature, and initial concentration on adsorption were investigated. Results revealed that maximum adsorption of metal ions are observed at pH of 6, a contact time of 60 min, and a temperature of 70 ºC. In addition, modification was to considerably impact the effectiveness of the adsorbent. The percentage removal of the metal ions on the modified (MCLR) and unmodified adsorbents (UCLR) followed increasing order of Co(II) ion on UCLR < Ni(II) ion on UCLR < Co(II) ion on the NaOH-modified adsorbent < Ni(II) ion on the NaOH-modified adsorbent. Equilibrium data were analyzed by the Langmuir and Freundlich isotherm models, and kinetic studies were carried out using pseudo-first-order and pseudo-second-order models. The adsorption data were well fitted to the Freundlich isotherm model equation, with correlation coefficient (R2) values greater than 0.950, and the pseudo-second-order kinetic model, with correlation coefficient (R2) values greater than 0.990. The monolayer adsorption capacities for modified C. lepidota residue and unmodified C. lepidota residue were 5.890 mg/g and 5.627 mg/g, respectively.

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