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Jurnal Ilmiah Sains
Published by Universitas Sam Ratulangi
ISSN : 14123770     EISSN : 25409840     DOI : https://doi.org/10.35799/jis.v22i2.40961
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Mathematics Physics
Jurnal Ilmiah Sains (Journal of Scientific Sciences) is the Journals Published by Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. Jurnal Ilmiah Sains Published Twice a Year, i.e April and October. Jurnal Ilmiah Sains  welcomes full research articles in the area of Mathematics and Natural Sciences from the following subject areas: Mathematics Statistics Computer Science Physics Chemistry Biology
Arjuna Subject : Umum - Umum
Articles 2 Documents
 1 
Search results for , issue "volume 26 issue 2, october 2026" : 2 Documents clear
Toxicity of Copper (Cu) on Abnormalities and Mortality of Tilapia Fish (Oreochromis niloticus L.) Prolarvae Wa Ode Harlis; Desti Yulia Saputri; Nurhayu Malik; Andi Septiana; Resman Resman
Jurnal Ilmiah Sains Volume 26 Issue 2, October 2026
Publisher : Sam Ratulangi University, Manado, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jis.v26i2.67205

Abstract

Contamination of the heavy metal Copper (Cu) in waters can inhibit fish growth by reducing the level of gonad maturity and closing the gill membranes. changes in activity, morphological abnormalities of the prolarvae and even death. This study aims to assess the toxicity of Copper (Cu) to tilapia fish prolarvae (Oreochromis niloticus L.). This research is a type of experimental research structured based on a Completely Randomized Design (CRD) pattern.  A total of 240 three-day-old tilapia prolarvae were divided into four treatment containers, namely Control, P1(2.0 mg/L), P2(2.5 mg/L), P3(3.0 mg/L), and each treatment consisted of three replications. Six hours after administration of copper, mortality parameters were observed, while prolarval abnormalities were observed after 48 hours.  Data were analyzed using one way ANOVA (Analysis of Varience) α=0.05. The results of the study showed that the average percentage of mortality for controls was (0%), P1(6.67%), P2(23.33%), P3(36.67%) while the forms of abnormalities found were kyphosis, lordosis, scoliosis and tail sticking. The research results show that copper (Cu) has a toxic effect because it increases the percentage of mortality and causes abnormalities in tilapia prolarvae (Oreochromis niloticus L.). Keywords: Copper (Cu); Oreochromis niloticus; prolarvae; toxicity
Antioxidant Activity of Clay Mask Stick with Premna serratifolia L. Extract Using Green Solvents Nurmala Sari; isnindar isnindar; Nera Umilia Purwanti
Jurnal Ilmiah Sains Volume 26 Issue 2, October 2026
Publisher : Sam Ratulangi University, Manado, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jis.v26i2.67596

Abstract

Buas-buas leaves are a medicinal plant containing flavonoids and phenolic compounds with potential antioxidant activity to protect the skin from damage caused by free radicals. The use of green solvents has emerged as an alternative in developing safer and more sustainable cosmetic formulations. This study aimed to determine the IC₅₀ value of buas-buas leaf extracts obtained using green solvents and to formulate the most active extract into a clay mask stick preparation. Extraction was carried out using the MAE method with natural honey mixed with distilled water (1:20) and NADES based on citric acid and glucose (1:1). Antioxidant activity of both extracts and formulations was evaluated using the DPPH method to determine IC₅₀ values. The extract with the best activity was then formulated and further evaluated for physical and chemical stability using a cycling test method. The results showed that the NADES extract exhibited very strong antioxidant activity with an IC₅₀ value of 31.53 ppm, while the natural honey extract showed moderate activity with an IC₅₀ of 108.53 ppm. The clay mask stick formulations on day 0 had IC₅₀ values of 207.94 ppm (F1) and 171.57 ppm (F2), categorized as weak, and 110.88 ppm (F3), categorized as moderate. After six cycles of the cycling test, IC₅₀ values increased to 434.28 ppm (F1) and 312.55 ppm (F2), categorized as very weak, and 208.48 ppm (F3), categorized as weak, indicating decreased antioxidant activity after accelerated storage. However, all formulations remained physically stable and had pH values within a safe range for skin.

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