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Journal of Global Pharma Technology
Published by Universitas Udayana
ISSN : 09758542     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
ournal of Global Pharma Technology is a monthly, open access, Peer review journal of Pharmacy published by JGPT Journal publishes peer-reviewed original research papers, case reports and systematic reviews. The journal allows free access to its contents, which is likely to attract more readers and citations to articles published in JGPT. JGPT publishes original research work that contributes significantly to the scientific knowledge in pharmacy and pharmaceutical sciences- Pharmaceutics, Novel Drug Delivery, Pharmaceutical Technology, Cosmeticology, Biopharmaceutics and Pharmacokinetics, Pharmacognosy, Natural Product Research, Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmaceutical Analysis, Pharmacology, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics, Biotechnology and Applied Computer Technology. For this purpose we would like to ask you to contribute your excellent papers in pharmaceutical sciences.
Arjuna Subject : Kedokteran - Onkologi
Articles 93 Documents
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Search results for , issue "Volume 11 Issue 05." : 93 Documents clear
A Nobel Chemiluminescence and Fluorescence Energy Transfer Three Inlets Cell for the Determination of Hydrogen Peroxide via Continuous Flow Injection Analysis Nagam Shakir Turkie Al-Awadi
Journal of Global Pharma Technology Volume 11 Issue 05.
Publisher : Journal of Global Pharma Technology

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Abstract

The study presents a sensitive, fast and easy assay method that was developed for determination of hydrogen peroxide  via continuous mode of FIA i.e. new noble chemiluminescence and fluorescence cell with specially designed unit cell of three inlets and  one outlet  to measure a total luminescence which include the chemiluminescence generated and the fluorescence that was created by the in situ radiation of the released chemiluminescence light using fluorescence molecule which gives an emission spectrum at 530 nm The method is based on the use of fluorescent molecule as an acceptor fluorophore where by it is irradiated internally and instantly when the generation of luminol chemiluminescence light is used as internal source for irradiation of fluorescein molecule to give fluorescence light , this reaction and analysis occur in a specially homemade  designed cell. Experimental parameters that lead to optimum concentrations of all chemicals and physicals variables .A sample volume of 75 μL was used throughout the whole work. It was found that the linear working range was between 20-500 μmol. L-1, with correlation coefficient of 0.9969 and limit of detection L.O.D. (S/N =3) 1.275ng/sample by using step wise dilution of the minimum concentration that was achieved by the calibration graph. Repeatability of less than 0.25% for ten successive injections of 250 μmol.L-1 & 400μmol .L-1 of hydrogen peroxide .The method was applied successively in determination of hydrogen peroxide in some pharmaceutical disinfectants.Keywords: Chemiluminescence, Flow injection analysis, Hydrogen peroxide, In situ fluorescence.
Direct Molecular Detection and Phylogenetic Relationship of Staphylococcus aurous from Mastitis Milk in Sheep and Cows Esraa T.M.
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

The present study included the direct detection of Staphylococcus aurous isolated from milk of sheep and cows  infected by mastitis based 16SrRNA gene by PCR technique and study the phylogenetic relationship analysis based on spa gene of using DNA sequence method.  The study includes collected 32 sheep and 45 cow’s mastitis milk samples. These samples were submitted to milk DNA extraction, and then performed PCR technique. The PCR results were showed significant at P value: (< 0.05) in prevalence between sheep and cow Staphylococcus aurous positive isolates at (5/15.5%) and (14/31.1%) respectively.  DNA sequencing of partial sequence of spa gene was showed Staphylococcus aureus sheep isolate was genetic relationship analysis different than cow isolate and related to NCBI-Blast Staphylococcus aureus isolate H13939 (AM407304.1) at  (99%) homology sequence identity and NCBI-Blast Staphylococcus aureus strain AO124 (JX912490.1) at  (94%) homology sequence identity total genetic change (0.005). In conclusion, Staphylococcus aurous isolated from mastitis milk in sheep and cows were not related genetically. Therefore, Spa gene analysis was provides an efficient method for investigating phylogenetic relationships among different clinical isolates and can be useful for monitoring distribution of pathogen related to public health.Keywords: Staphylococcus aureus, Milk, Sheep, Cow, PCR, 16SrRNA, spa gene.
Compensatory and Adaptive Features of the Cerebellum under Stress Alexey Rozhentsev
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

The paper discusses the results of the study of compensatory and adaptive changes in rat cerebellar tissues under stress. As a stress factor, the experiment simulated (hypobaric) hypoxia. Since hypoxia remains one of the central issues in the modern medical science, it is directly or indirectly related to human diseases of various etiologies. To identify the features of compensatory and adaptive changes in the rat cerebellum under the action of hypoxia, we conducted comprehensive histological and histochemical studies. At the early stages of hypoxia, changes in the cerebellar tissue could be found, that were characterized by a significant increase in the volume of intracellular structures. With a prolonged hypoxia, a decrease in the volume of intracellular structures was observed which, in turn, entailed a decrease in total glycosaminoglycans and a change in the ratio of sulfated and non-sulfated glycosaminoglycans. In addition, we noted cerebellar neuron migration into the molecular layer.  The experiment revealed that hypoxia has a significant effect on the intensity of metabolic processes in cells, with the generation of intermediate metabolic products leading to a change in pH.Keywords: Hypoxia, Cerebellum, Rats, Compensatory and adaptive changes, Glycosaminoglycans.
Relationship between HCMV and Diabetic Mellitus Type 2 of Elderly Patients in Al-Najaf Governorate Elham Jawad Kadhum
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

The sudden rise mellitus (DM2) and related complications go along with increasing evidence of clinically major age and gender differences. The aim of current study was to study the relationship between the virus and diabetes mellitus type2 through detection of human cytomegalovirus (HCMV) infection in DM2 patients in Najaf governorate by detection of human cytomegalovirus DNA in blood samples by using of PCR technique. Blood samples were collected from 93 diabetic patients randomly. Regarding PCR technique, the study showed that among 93 diabetic patients gave positive results in 39 patients(42%); In contrast, in control group PCR positive result was only 4.4% (4 out of 90) with significant differences . This study concluded that the higher prevalence of HCMV DNA in DM2 patients comparing with normal individuals, which means that patients with DM2 were at high risk for HCMV infections.Keywords: HCMV, DM2, PCR.
Synthesis, Characterization and Study of the Biological Activity of New Metal Complexes of Ligand[2-(3-benzoylthioureido)-3-(-4-hydroxyphenyl) Propanoic Acid (BHP) Enass Jasim Waheed
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

In this paper, series of new complexes of Manganese(II), Cobalt(II), Nickel (II) Cupper(II) Zinc(II), Cadmium(II) and Mercury (II) are prepared from the new ligand  [2-(3-benzoylthioureido)-3-(-4-hydroxyphenyl) propanoic acid (BHP) derived from tyrosine and benzoylisothiocyanate .Chemical structures are obtained from their 1 H, 13CNMR spectra (for BHP), elemental microanalyses, molar conductance, FTIR, UV–Vis, magnetic susceptibility in addition to TGA/DTG and DSC analysis, the suggested geometry for all complexes was tetrahedral. The biological activity of BHP and its complexes has been extensively studied against two bacterial species Staphylococcus aurous (G+) and Escherichia coli (G-) by agar-well diffusion technique, where Mn(II), Co(II), Ni (II)  complexes showed greater biological activity compared to ligand. Keywords: Tyrosine, Metal complexes, Biological activity.
Evaluation of the Effects of Different Solvents Extracts of Tribulus Terrestris Obtained Via Ultrasonic Extraction on Human Asthenozoosperm Activation In Vitro Hikmat Fakhri Wassef
Journal of Global Pharma Technology Volume 11 Issue 05.
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Objective: This study was designed to evaluate the activity of different fractions of Tribulus terrestris Tt obtained via 20KHZ ultrasonic technique for different periods of extraction time and different solvents on different asthenozoospermic parameters in vitro. Material and method: The study was conducted on seventy-five asthenozoosperic patients. The sperm samples were activated in vitro (after liquefaction) with different doses of the extracts by using a direct simple layer method (DSLM) technique. Grades of sperm movement and sperm morphology were the main parameters tested. Results: Aqueous extract for 15 min. at a dose 0.02% showed the highest activity. Less activity was obtained by exposure of the sperm samples to a dose 0.002% aqueous acetonitrile (AA) extract for 15 min., 0.0002% dose of Ethanol (EtOH) and Methanol (MeOH) extracts for 15 min., 0.002% dose of (AA) extract for 15 min. and 0.002% dose of (EtOH) and (MeOH) extracts for 60 min. Exposure of asthenozoospermic samples to a dose of 0.0002% of (AA) extract for 60 min. gave the lowest activity. Conclusion: These finding indicated that the use of ultrasonically assisted extraction can obviously improve the activity of Tt extracts on asthenozoospermic patient’s parameters including the grades of sperm motility and sperm morphology.Keywords: Asthenozoospermia; Ethanol (EtOH); In vitro activation; Methanol (MeOH); Tribulus terrestris Tt; ultrasound extraction.
Synthesis, Antibacterial Evaluation and Docking Study of Some New Fused Pyrido-Pyrimidine and Naphthyridine Cycles Hamid H. Mohammed
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

New fused pyrido-pyrimidine and naphthyridine cycles were synthesized and characterized using spectral analysis. Initially, 2-cyano-N-(1-phenylethylidene) acetohydrazide (2) was prepared from the reaction of cyanoacetic acid hydrazide (1) with acetophenone then cyclized to 4,6-diamino-2-oxo-1-((1-phenylethylidene)amino)-1,2-dihy-dropyridine-3-carbonitrile (3) by the action of malononitrile and triethyl amine in dioxin. Fused pyridopyrimidine cycles (4-6) were obtained from the reaction of compound 3 with formic acid, glacial acetic acid and propionic acid, respectively in the presence of POCl3 as a catalyst. The novel cycles (7-9) were synthesized from the cyclization reaction of compound 3 with benzoyl chloride, phenylisocyanate and phenylisothiocyanate in presence of pyridine. Naphthyridine cycles (10-13) were obtained by cyclization of compound 3 with some of aliphatic ketones namely; acetone, cyclohexanone, 2-butanone and 4-methyl-2-pentanone, respectively in presence of FeCl3 as catalyst. The antimicrobial activity of the synthesized derivatives was evaluated against several bacterial species as well as candida albicans. Docking study was also achieved to explore the binding affinity of the potent discovered hit (9) inside the binding pocket of glucosamine-6-phosphate synthase, a target enzyme for the antimicrobial agents.Keywords: Naphthyridine Cycles; Fused Pyrido-Pyrimidine; Antibacterial.
Different Technical Variables Affecting Noni (Morinda Citrifolia L.) Wine Fermentation N. P. Minh
Journal of Global Pharma Technology Volume 11 Issue 05.
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Abstract

Noni (Morinda citrifolia L.) is one of the most important fruit which was widely used for its health restorative properties. It has been used an herbal medicine in various ailments. This fruit is considered a natural antioxidant. It can prevent cancer, heart disease, diabetes, cognitive dysfunction, hypertension etc., and maintain overall good health. Over the years, its popularity has been diminished due to unpleasant smell from the ripened fruit. There is limited study mentioning to processing of this functional fruit. Therefore we explored a wine fermentation from noni fruit by focusing on the effect of different variables such as sugar supplementation, yeast ratio inculation, fermentation time and temperature in the primary fermentation, and fermentation time and temperature in the secondary fermentation to noni wine quality (%v/v alcohol; g/l acidity; oBrix residual sugar; sensory score). Our results proved that the primary fermentation should be conducted with 15% sugar supplementation, 0.15% Sacchromyces cerevisiae at temperature 29.0oC in 10 days. The secondary fermentation was adequate in 9.5oC for 3 weeks to get a pleasant flavor and aroma. Noni wine also evaluated the antioxidant capacity and total phenolic content. Noni wine could be considered as a good source of antioxidants and phenolics ideal for daily healthy consumption.Keywords: Noni, Wine, Fermentation, Sacchromyces cerevisiae, Antioxidant, Phenolic.
Determination of the Genotoxic Potential of Annatto Seed by Single Cell Gel Electrophoresis in Layer- Hen Bushra M.W. Al-Obaidi
Journal of Global Pharma Technology Volume 11 Issue 05.
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The aimed of this study is to investigation the toxicity of annatto seeds added to the diet of layer hens for different level. A total of 240 Lohmann hens 67 week of ages for 12 weeks were distributed randomized blocks into four treatments (60 hens) with three replicates 20 hens of each. The following treatments were applied: T1- control feed on based die; T2- feed on Annatto seed at 75gm/100kg, T3- feed on Annatto seed at 150gm/100kg, T4- feed on Annatto seed at 225gm/100gm. In the end of study blood samples were collected from 9 birds from each group from the wing vein in a test tube with coagulant for cytogenetic parameters. The result demonstrated that 2nd group recorded maximum significant (p≤0.05) differences compared to other nourished  groups in all comet assay parameters(%DNA in head, Tail  length,% DNA in tail, Tail moment and Olive tail moment) then followed by T3 regressed maximum significant (p≤0.05) differences of comet length. T1, T4 recorded a significant (p≤ 0.05) decrement in comet assay parameter respectively compared with other studied groups. The addition of Annatto seed at 225gm / 100kg  to the diet of layer hens has an adverse effect on blood lymphocyte and cases highly damage of DNA, while added at level 75gm/kg(T2) and 150 gm/kg(T3)which protect lymphocyte from damage  as shown in comet assay parameters.Keywords: Annatto seed, Single Cell Gel Electrophoresis (comet assay), Layer.
Antibacterial Activity of Nisin-Silver Nanoparticles on Staphylococcus epidermidis and Therapeutic Effects in Experimental Skin Infection in Mice Ahmed Qassim Al-Awadi
Journal of Global Pharma Technology Volume 11 Issue 05.
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Today technology using nanoparticle when treatment pathogentic microorganism and we focused on this here .It was found that the species of Staphylococcus epidermidis used in present study were sensitive to Levofloxacin .The aim of report effect (Nisin-Silver Nanoparticles) on ability of complete healing injury comparing using Nisin only. In vivo study revealed that silver nanopasrticles treatment of S. epidermidis contaminated injured skin showed good healing process contain complete regeneration of the epithelial cells of the epidermis and good prognosis and increase of cellulartiy of the dermal content compared with untreated group. In conclusion, treatment of skin infected with S.epidermidis using silver nanoparticles at different concentration may limit the skin damage, localized the lesion to the incision site and enhance the healing process.Key words: Staphylococcus epidermidis, Nisin A, silver nano particles.

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