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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 9 Documents
 1 
Search results for , issue "Vol 16, No 1 (1998)" : 9 Documents clear
EFFECTS OF ACTIVE GUMBORO VACCINE OF INTERMEDIATE STRAIN HIGH PATHOGENICITY ON THE MORPHOLOGY OF BURSA FABRICIUS AND THE IMMUNE RESPONSE OF BROILER TO VACCINATION AGAINST NEWCASTLE DISEASE Charles Rangga Tabbu
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8606

Abstract

This experiment was designed to study the effects of active Gumboro vaccine of high pathogenicity on the morphology of bursa Fabricius and the immune response of broiler to vaccination against Newcastle disease (ND). This experiment was conducted in 330 broilers, which were vaccinated with aviive Gumboro vaccine of high, intermediate and low pathogenicity (control) at the age of 12 days. Experimental chickens were also vaccinated against ND at the age of 4 and 18 days. Diameter of bursas were measured at day 1st through 7th, 14th, 21th, 28th, 35th post Gumboro vaccination. Evaluation of hemaggutination inhibition (HI) liters against ND virus (NDV) were done only at day 7th, 14th, 2lth, 28th and 35th. Samples of bursa were stained with the method of hematoxylin and eosin (H & E). Pathologic evaluation revealed lesions in the bursa Fabricius of chickens vaccinated with Gumboro vaccine of high pathogenicity as well as changes in the diameter of bursa of lesions in this organ. The HI titers against NDV were lower in the group of chickens vaccinated with Gumboro vaccine of high pathogenicity compared with them of other groups. Results indicated that active Gumboro vaccine of high pathogenicity caused lesions in the bursa,which were similar to lesions caused by exposure to field Gumboro virus.
EFFECTS OF SILVER INDUSTRY WASTE WATER IN KOTAGEDE, YOGYAKARTA ON HAEMTOLOGlCAL AND HISTOPATHOLOGICAL CHANGES IN EXPERIMENTAL RATS (RATTUS NORVEGICUS) Bambang Hariono
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8607

Abstract

Thirty male rats, 2,5 months old with average body weight of 92 grams were used as experimental animals.The experiment rats were adapted before for one week. The animal grouping was basedon uniformity of body weight gain, Thecontrol group consist of 15 animals and 15 animals for treated group. The treated group was given silver industry waste water from Kotagede, Yogyakana orally ad libitum the control group was supplied drinking tap water ad libitum. Blood samples via plexus retroorhitalis vein at medial cantus of the eye were taken for routine heamiolafiical examinations, clinical symptoms and body weight gain were also recorded. After treatment, for about 12 weeks, all experimental animals were sacrificed, necropsied, then the samples of brain, spinal cord, lung, tesiis. liver, and kidney were taken for histopathological preparations. The concentration of metals (especially Ag and Cu) in the liver, silver industry waste water, well water, Tap water, and soil samples (which [he silver industry waste water was discarded) were analyzed. The results of the experiment indicated thai the silver industry waste water administration in long period caused decreasing of the rat growth rate with clinical symptoms e.g. thinness,dullnessof the fur, anaemic, depression and nerve symptoms including foot paralysis. Statistical calculation showed there were not significantly changes on hematological profile between the control and treated groups, it could be due to severe dehydratation level occured, so there were reducing of blood volume. This finding was similar to leucocyte and differential counts, but there tended to decrease at week-12. There could be due 10 bone marrow depression which were caused by heavy metal intoxication. Microscopical examinaton showed there were thickness of alveoli septa, congestion, enlargement of sinusoids and infiltration of mononuclear cells around the liver centralvein, degeneration and necrosis of tubule cells, enlargement of tubule lumen and Bowman capsule space, infiltration of monoclear cells and haemorrhage performance. There were a few Purkinje cells showed more denses and the sTain more absorpted intensively (the same findings occuredin the nerve cells of spinal cord), it could be possible that there were cell structural changes, degeneration and necrosis of The nerve cells especially in the spinal cord tissues. The silver, copper and chrome concentrations in the silver industry waste water and soils samples were too high, it could be as potential sources for environmental pollution. The concentration of these metals in the tap water and well water were not detected yet.
IMMUNOGENICITY AND PROTECTIVITY OF THE OUTER MEMBRANE PROTEIN BANDS OF PASTEURELLA MULTOCIDA ISOLATE U-6 Ignatius Mulyadi; Widya Asmara; B. Sardjono
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8608

Abstract

The most immunogenic and protective fraction of outer membrane proteins of Pasieurella multacida has not well understood. This study was done to find our the immunogenicity and protectivity of outer membrane protein fractions of Pasteurella multicida isolat U-6 in mice. The organism was cultured in Luria Bertani broth. The outer membrane proteins were prepared according 10 Snipes et al., (1988), Electrophoresis of the protein was carried out in 10% of sodium dodecy 1-su I ph ale poly cry 1 amide gel electrophoresis, and stained with 0.3% Coomasie brilliant blue. The result indicated that there arc many protein bands with 5 major protein bands, those are having molecular weight of 20,1 kilo Dalton (kD), 27 kD, 36 kD, 52,7kD,and75 kD. Each protein bandof molecular weight of 27 kD, 36kD,and75kD was then cut, and homogenised, and was used as antigen to immunise the mice after added with adjuvant 1:1. Two weeks after immunisation all of the mice were bled and the sera were tested by ELISA. FourtyeigM hours from bleeding, ail of the immunised and control mice were challenged with 500 colony forming unit of virulent Pasteurefla mutticida isolate U-6, and observed their mortality. The result indicated thai the outer membrane protein bands of molecular weight of 36 kD and 75 kD were immunogenic (P>0,05), while the outer membrane protein band of 27 kD was less immunogenic (P>0.05), Band with moleculer weight of 27 kD produced 0% protection, bandof 36 kD produced 60% protection, and band of 75 kD produced 40% protection of mice challenged.
INTERPRETATION OF DIAGNOSTIC TESTS IN CLINICAL EPIDEMIOLOGY Bambang Sumiarto
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8609

Abstract

A test is process designed to detect a sign, substance tissue change, or response. The detection of signs or tissue abnormalities during [he diagnostic process, pregnancy diagnostic activities, and meat inspection findings, are also tests. Indeed, tests are used in virtually all problem solving activities and therefore the understanding of the principles of lest evaluation and interpretation are basic to many of practical veterinarian and researcher. Clinical epidemiology revolves around the use and interpretation of tests. 
ADHESION AND PHAGOCYTOSIS PROPERTIES OFSTREPTOCOCCUS EQUI SUBSP. ZOOEPIDEMICUS ISOLATES FROM INDONESIA Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8610

Abstract

An infectious diseases outbreak affecting pigs and monkeys in Bali in 1994, with case fatality rate (CFR) 75%. The disease widespread rapidly to the other district in Indonesia and developed serious problems. The causative agent involving the disease was Streptococcus equi subsp. zooepidemicus, a β-hemoIytic type of the streptococcus. The disease was consistently associated with meningitis, therefore the disease was named Streprococcal meningitis. The main aim of this study is todetect factors promoting the pathogenicity of the agent that could be showed by its characteristics and properties of the adhesions and phagocytosis. Eight and two isolates of Strepiacoccus equi subsp. zooepidemicus originated, from pigs and monkeys respectively were used in this study. The nature of bacterial surface proteins was characterized by hexadecane adherence test, hemaglutinating erythrocytes of various animals, adherence to epithelial cells and phagocytosis of the bacteria by polymorphonuclear leucocytes. The result of hexadecane adherence test showed that of the ten isolates oniy one culture of pigs isolates had a hydrophobic surface and the others were hedrophilic surface. The culture with hydrophobic surface agglutinated erythrocytes from chicken, rat and rabbit. This culture adhered in high numbers to epithelial cells and were more phagocytosed by polymorphonulear leucocytes. The cultures with hy drop hi lie surface showed no comparable reaction.
MONOCLONAL ANTIBODIES AGAINST OUTER MEMBRANE PROTEIN PASTEURELLA MULTOCIDA STRAIN DOMPU Sri Murwani; Widya Asmara
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8618

Abstract

Monoclonal antibodies were produced against outer membrane protein of Pasteurella multocida strain Dompu. The bacterial isolate was obtained from [he bacteria! collection held at Microbiology Laboratory, Faculty of Veterinary Medicine, Gadjah Mada University. Irs purity and patogenicily has beeen tested.Killed PatTeurella multocida were used ro immunized 7 female, 8-10 week old of Balb/ c mice. Cell fusion between limphocytes and myeloma cells (2:1) were carried out by PEG stimulation and bybrids were cultured in  HAT medium.The OMP were prepared from sonicated organism using centrifugallon in the presense of sarcosyL These antigens were than used to select antibody producing hvbridomas ELISA and cloning of hybridomas were done by serial dilution method.Four hybridomas clones were obtained, till producing monoclonal antibodies which reacted specifically against OMP Pasietirelta multacida stain Dompu.
THE CHANGES OF THE TESTOSTERONE HORMONE LEVEL DURING PREPUBERTAL PERIODE IN INDUCED HYPOTHYROIDISM RATS Teguh Budipitojo; Tri Wahyu Pangestiningsih
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8619

Abstract

The one the influence factors to the normal functions and testes growth is thyroid gland status. The knowledge about the influences of euthyroid, and hypenhyroid to thetesies growth will help to give more understanding of the normal lestes growth. The new born male rats were used todetect the influence of hypothyroidism to the testosterone level during prepubertal periode. Twenty primigravida pregnant rats (Rattus norvegicus albinus) were used. After birth, the male pups were divided inio controls and treatments group. Mothers of the treatment group were given 0,1% 6-n-prapyl-2-thiauracil (PTU), via drinking water, ad libitum, after birth until on day 8. On dary 27, 30, 37, 44, and 51, five control pups and 5 treatment pups were sacrificed. The blood from each pup were collected to detec serum testosteron hormone level using immunoradLometric assay (IRMA) method. The research showed that serum testosteron level ofthe; treated group increased on day 30 (P<0,05) and day 37(P<0,05). Theresultof This research showed that PTU induced hypothyroidism produced increasing serum testosterone level.
HISTOPATHOLOGICAL CHANGES OF SCHISTOSOMIASIS FROM NAPU VALLEY, CENTRAL SULAWESI, 1NDONESIA Kurniasih Kurniasih
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8620

Abstract

Schistosomiasis is an endemic disease caused by the infection Schistosoma Japonicum (Trematoda), can attack human and animals surrounding Lindu lake and Napu valley. Central Sulawesi, Indonesia. Five mice were naturally infected through subcuian with 75 cercariae of Schistosoma japonicum emerged from snail, Oncomelanic hupensis lindoensis, which were found in the Napu valley, Central Sulawesi. Mice showed a weakness, anorrhexia, stomach ache,and died in the fifth, sixth and seventh week posiinfection. At autopsy, mice had ascites, yellowish nodules on the surface of lung, liver, serosa of the small intestine, and mesenterium. Histolagically, there were severe necrosis and the granolomatous inflammation of the liver, the spleen, the lung, the pancreas, and the small intestine.
THE EFFECT OF FEVER ON ACETYLATION RATIO AND SULFADIAZINE ELEMINATION KINETICS OF LOCAL SHEEP (Ovis aries) Ida Tjahajati; Subronto Prodjoharjono; Budiono Santoso
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8621

Abstract

A Study has been conducted to identify effect of fever towards acetylafion ratio and eleminatkm kinetics of sulfadiazine in sheep. The siudy consist 10 healthy sheep using cross­over design. Each animal was treated twice with 40 days interval. In the first treatment the sheep were given 2 gram of sulfadiazine and 0.4 gram pyrimethamine. In the second treatment the animals were treated fever induction prior to treatment using 2 gram of sulfadiazin and 0.4 gram pyrimethamine. Blood samples were taken From jugular vein in hour of 0, 0.08.0.25, 0.5, 1,2,4,6,9.12, 18, and 24. Plasma sulfadiazine levels were analyzed using Bratton-Marshall Methode. Sulfadiazin parameter was calculated using Stripe program. Theresults of the study revealed that fever did not significantly (P>0,05) increased the aceiylation ratio and inhibited elemination of sulfadiazine.

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