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All Journal Jurnal Sain Veteriner Jurnal Veteriner Majalah Kedokteran Gigi Indonesia Indonesian Journal of Biotechnology Journal of Tropical Biodiversity and Biotechnology Jurnal Medik Veteriner Bioscientia Medicina : Journal of Biomedicine and Translational Research JURNAL KAJIAN VETERINER IGKOJEI: Jurnal Pengabdian Masyarakat Bioscientia Medicina : Journal of Biomedicine and Translational Research Livestock and Animal Research Jurnal Kedokteran Hewan
Tri Wahyu Pangestiningsih
Departemen Anatomi, Fakultas Kedokteran Hewan Universitas Gadjah Mada Jl. Fauna No. 2 Karangmalang, Yogyakarata
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary

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The Distribution of MAP-2 Phosphorylation in Cerebral Cortex of Long-Tailed Monkey Fetuses (Macaca fascicularis) in the Last Trimester of Gestation Pangestiningsih, Tri Wahyu; Irawan, Vidya; Sulistiawati, Erni
Indonesian Journal of Biotechnology Vol 15, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.706 KB)

Abstract

Memories are storage in cholinoceptive cells, the cells which are enriched with microtubule-associated protein 2 (MAP-2) that localized in the neuronal dendrite and the cell bodies. Phosphorylation of MAP-2 may increase memory with reduce stability of dendrite by altered dendrite length and lead new side-branches of neuronal as a neuronal plasticity processes in cerebral cortex. The aim of this research is to study the distribution of MAP-2 phosphorylation neurons in cerebral cortex of long-tailed macaques in the third semester of gestationalimmunohistochemically using avidin biotin conjugated complex method. Neurons MAP-2 phosphorylation immunoreactive were located in dendrites and cell bodies, mostly in pyramidal neurons of cerebral cortex. Intensity of MAP-2 phosphorylation immunoreactivity in layer V were stronger than another layer and the neurons that very intensely stained were the pyramidal cells in frontal and parietal lobes, that was suggested that neurons in this areas more responsive to neuroplasticity. From the results we concluded that MAP-2 phosphorylation already distributed in the cerebral cortex of long-tailed macaque fetuses at the last trimester of gestation, mostly in the pyramidal cells of layer V that is suggested plays a role for preparation of memoryformation.Keywords: fetus, long-tailed monkey, cerebral cortex, memory, MAP-2 phosphorylation
Histological Features of Catecholaminergic Neuron in Substantia Nigra Induced by Paraquat Dichloride (1,1-dimethyl-4,4 bipyridinium) in Wistar Rat as A Model of Parkinson Disease Pangestiningsih, Tri Wahyu; Wendo, Woro Danur; Selan, Yulfia Nelymalik; Amalo, Filphin Adolfin; Ndaong, Nemay Anggadewi; Lenda, Victor
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.756 KB)

Abstract

Paraquat dichloride has been used by farmers as a herbicide to kill the grass. On the other hand, paraquatdichloride is harmful if enters to the body, causing Parkinson’s disease, since it is disrupting dopamineproduction in the substantia nigra pars compacta or dopamine pathways Nigro striatal pathway. The studywas done to fi nd out the histological changes of catecholaminergic neurons and Nigro striatal pathway causedby paraquat dichloride treatment in Wistar rats as a model of Parkinson’s disease.Twenty-two Wistar rats 3,5 months old were divided into 4 groups, 5 rats each. Group I (control group)were injected with aquabidest, while groups II, III, and IV were injected intraperitoneally with paraquatdichloride in aquabidest, with the dosage 5 , 10 and 15 mg/kg bw respectively. The rats were injected onceper week for 6 weeks. Three days after the last injection, the rats were anesthetized using xylasin (2 mg/kg)and ketamine (20 mg/kg) intramuscularly, and then were intracardiac perfused using physiological saline asprerinse solution, followed by 10% buffered formalin solution as a fi xative. After animals were fi xed, the brainswere removed and embedded in paraffi n block and cut in 12 μm thickness for immunohistochemistry stainingusing tyrosine hydroxylase antibody. The results of staining then were observed under light microscope andanalyzed descriptively.The results showed that the catecholaminergic neurons were distributed in the substantia nigrapars compacta in all treatment groups, however, the cell density were found decreased only in group IV.Catecholaminergic neurons appear in the bipolar and multipolar form, while dopamine ‘Nigro striatal pathway’was found exist in all treatment groups. From our study, histologycally the decreased of catecholaminergicneurons is only found in rats that received paraquat dichloride in dose 15 mg/kg bw for 6 weeks.
The Distribution of MAP-2 Phosphorylation in Cerebral Cortex of Long-Tailed Monkey Fetuses (Macaca fascicularis) in the Last Trimester of Gestation Tri Wahyu Pangestiningsih; Vidya Irawan; Erni Sulistiawati
Indonesian Journal of Biotechnology Vol 15, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.706 KB) | DOI: 10.22146/ijbiotech.7827

Abstract

Memories are storage in cholinoceptive cells, the cells which are enriched with microtubule-associated protein 2 (MAP-2) that localized in the neuronal dendrite and the cell bodies. Phosphorylation of MAP-2 may increase memory with reduce stability of dendrite by altered dendrite length and lead new side-branches of neuronal as a neuronal plasticity processes in cerebral cortex. The aim of this research is to study the distribution of MAP-2 phosphorylation neurons in cerebral cortex of long-tailed macaques in the third semester of gestationalimmunohistochemically using avidin biotin conjugated complex method. Neurons MAP-2 phosphorylation immunoreactive were located in dendrites and cell bodies, mostly in pyramidal neurons of cerebral cortex. Intensity of MAP-2 phosphorylation immunoreactivity in layer V were stronger than another layer and the neurons that very intensely stained were the pyramidal cells in frontal and parietal lobes, that was suggested that neurons in this areas more responsive to neuroplasticity. From the results we concluded that MAP-2 phosphorylation already distributed in the cerebral cortex of long-tailed macaque fetuses at the last trimester of gestation, mostly in the pyramidal cells of layer V that is suggested plays a role for preparation of memoryformation.Keywords: fetus, long-tailed monkey, cerebral cortex, memory, MAP-2 phosphorylation
Histological Features of Catecholaminergic Neuron in Substantia Nigra Induced by Paraquat Dichloride (1,1-dimethyl-4,4 bipyridinium) in Wistar Rat as A Model of Parkinson Disease Tri Wahyu Pangestiningsih; Woro Danur Wendo; Yulfia Nelymalik Selan; Filphin Adolfin Amalo; Nemay Anggadewi Ndaong; Victor Lenda
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.756 KB) | DOI: 10.22146/ijbiotech.8638

Abstract

Paraquat dichloride has been used by farmers as a herbicide to kill the grass. On the other hand, paraquatdichloride is harmful if enters to the body, causing Parkinson’s disease, since it is disrupting dopamineproduction in the substantia nigra pars compacta or dopamine pathways Nigro striatal pathway. The studywas done to fi nd out the histological changes of catecholaminergic neurons and Nigro striatal pathway causedby paraquat dichloride treatment in Wistar rats as a model of Parkinson’s disease.Twenty-two Wistar rats 3,5 months old were divided into 4 groups, 5 rats each. Group I (control group)were injected with aquabidest, while groups II, III, and IV were injected intraperitoneally with paraquatdichloride in aquabidest, with the dosage 5 , 10 and 15 mg/kg bw respectively. The rats were injected onceper week for 6 weeks. Three days after the last injection, the rats were anesthetized using xylasin (2 mg/kg)and ketamine (20 mg/kg) intramuscularly, and then were intracardiac perfused using physiological saline asprerinse solution, followed by 10% buffered formalin solution as a fi xative. After animals were fi xed, the brainswere removed and embedded in paraffi n block and cut in 12 μm thickness for immunohistochemistry stainingusing tyrosine hydroxylase antibody. The results of staining then were observed under light microscope andanalyzed descriptively.The results showed that the catecholaminergic neurons were distributed in the substantia nigrapars compacta in all treatment groups, however, the cell density were found decreased only in group IV.Catecholaminergic neurons appear in the bipolar and multipolar form, while dopamine ‘Nigro striatal pathway’was found exist in all treatment groups. From our study, histologycally the decreased of catecholaminergicneurons is only found in rats that received paraquat dichloride in dose 15 mg/kg bw for 6 weeks.
DISTRIBUSI SARAF PADA EPITEL OLFAKTORIUS KALONG KAPATUK (Pteropus vampyrus) DAN LASIWEN DEIGNAN (Myotis horsfieldii) BERDASARKAN EKSPRESI NEURON-SPECIFIC ENOLASE (NSE) Yosephine Nicolory Paula; Soehartini Jatman; . Ariana; Tri Wahyu Pangestiningsih; Teguh Budipitojo
Jurnal Sain Veteriner Vol 25, No 1 (2007): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5391.275 KB) | DOI: 10.22146/jsv.277

Abstract

Kelelawar merupakan salah satu keanekaragaman dan kekayaan satwa di Indonesia. Adanya keanekaragaman jenis makanan maupun habitat kelelawar menyebabkan terjadinya perbedaan dalam mencari dan cara makan dari berbagai jenis kelelawar. Keterkaitan antara struktur indra penciuman dengan jenis dan cara makan kelelawar sangat menarik untuk dieksplorasi, karena sampai saat ini sangat sedikit data mengenai hal tersebut. Penelitian ini menggunakan 3 ekor kalong betina dewasa dan 3 ekor lasiwen betina dewasa, sebagai sampel. Hidung sebagai sampel, kemudian dibuat preparat histologi dengan metode parafin dan  pewarnaan imunohistokimia terhadap ekspresi NSE. Hasil pewarnaan imunohistokimia menunjukkan bahwa imunoreaktivitas NSE pada lasiwen terindentifikasi dalam epitel olfaktorius di kedua sisi septum nasalis, konka dorsalis dan beberapa fila olfaktoria. Pada kalong tidak terindentifikasi adanya imunoreaktivitas NSE.Kata kunci: saraf, epitel olfaktorius, kalong, lasiwen, Neuron-Specific Enolase(NSE)
Kajian Imunohistokimia Perkembangan Bentuk Neuron Atekolaminergik Pada Area Postrema Monyet Ekor Panjang (Macaca fascicularis)= Morphological Development of Catecholaminergic Neurons in the Area Postrema of Long-tailed.. Tri Wahyu Pangestiningsih; Koeswinarning Sigit; Dondin Sajuthi; Nurhidayat .
Jurnal Sain Veteriner Vol 24, No 1 (2006): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2980.906 KB) | DOI: 10.22146/jsv.354

Abstract

rea postrema merupakan sepasang penonjolan ke dorsal pada bagian kaudal medula oblongata yang berbatasan dengan ventrikel IV. Area ini berperan sebagai chemoreceptor trigger zone (CTZ) pada proses muntah yang juga melibatkan neuron katekolaminergik (neuron KA). Pada kemoterapi penderita kanker, fungsi AP sebagai CTZ diupayakan untuk ditekan agar tidal( ada refleks muntah pada pasien. Dalam rangka lebih memahami neuron KA tersebut, dalam penelitian ini dilakukan pengamatan perkembangan bentuk neuron KA di area postrema (AP) monyet ekor panjang (MEP) mulai fetus (F) umur 40 sampai anak (P) umur 105 hari secara imunohistokimia menggunakan antibodi terhadap enzim tirosin hidroksilase (TH). Hasil penelitian memperlihatkan neuron KA di medula oblongata belwn terlihat pada F40, dan bare dijumpai pada F55 di daerah bakal AP, bentuk bulat dengan inti besar dengan sitoplasma sedikit yang merupakan ciri perkembangan awal bentuk neuron. Prosesus sitoplasma yang pendek pada mulai ditemukan pada F85, dan neuron KA di AP berubah menjadi bipolar pada F100 yang merupakan tanda perkembangan menengah bentuk neuron. Dengan bertambahnya umur, prosesus sitoplasma neuron KA bipolar bertambah panjang yang merupakan ciri tingkat perkembangan bentuk akhir dan ditemukan dominan di AP pada P105. Dari hasil penelitian ini dapat disimpulkan bahwa neuron KA di AP pada MEP berada dalam stadium perkembangan bentuk awal dan menengah selama masa prenatal, dan stadium perkembangan lanjut menuju ke perkembangan akhir terjadi pada masa postnatal.
Kuantitas Dan Kualitas Sel Telur Anjing Lokal Dari Berbagai Stadium Estrus Yuda Heru Fibrianto; Tri Wahyu Pangestiningsih; Amelia Hanna; Pudji Astuti; ClaudeM Mona Airin; Asyhari .; Amrullah Anindito; Nuraini Rahmawati; Koko Kurniawan; Pradityo Yoga Wibowo
Jurnal Sain Veteriner Vol 26, No 1 (2008): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1310.87 KB) | DOI: 10.22146/jsv.411

Abstract

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Perbandingan Pengaruh Penambahan Cairan Folikel Sapi dengan Babi terhadap Maturasi Oosit Anjing (Canis familiarsis) secara in Vitro dari Stadium Estrus Ovarium Yuda Heru Fibrianto; Tri Wahyu Pangestiningsih; Amelia Hana; Claude Mona Airin; Nuraini Rahmawati
Jurnal Sain Veteriner Vol 27, No 2 (2009): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1847.065 KB) | DOI: 10.22146/jsv.427

Abstract

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Pemasakan Oosit Anjing (Canis familiarsis pada Stadium Anestrus dan Diestrus pada Media Maturasi yang Diperkaya dengan Cairan Folikel Sapi 10% dengan Penambahan Follicle Stimulating Hormone (FSH) dan Luteotropic Hormone (LH) Yuda Heru Fibrianto; Amelia Hana; Tri Wahyu Pangestiningsih; Pradityo Yoga Wibowo; Claude Mona Airin
Jurnal Sain Veteriner Vol 28, No 1 (2010): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4904.308 KB) | DOI: 10.22146/jsv.430

Abstract

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Developmental Anatomy of Cerebellum of Long-Tailed Macaque (Macaca fascicularis) at the First Trimester of Gestation Tri Wahyu Pangestiningsih; Irene Linda Megawati Saputra; Agnya Sinung Suminar
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4578.798 KB) | DOI: 10.22146/jsv.5429

Abstract

Long tailed macaque was one of animal models in biomedical research because it has  many similarities with humans, both anatomical and physiological properties. There were many research about cerebellum associated with its role in the coordination of muscle activity. Understanding of normal development of cerebellum long tailed macaque may help to understand about the development in human cerebellum and its abnormalities. Embryonic and fetal brain samples were obtained through caesarean section and were  then made for histological preparation stained with cresyl violet. Staining results were observed using a microscope with a digital camera. Images obtained are processed by graphics software Adobe Photoshop CS 8.0. Cerebellum Macaca fascicularis Ed40 showed the isthmus and rhombic lip that were composed of ventricular layer, mantle layer, and marginal layer. Cerebellum Macaca fascicularis Fd55 showed future lobes and future  fissures, but the cortex and medulla are not bounded clear. The cortex consisted of the external granular layer, neuroblast basket, and neuroblast stellate, while the  medulla consisted of neuroblast deep cerebellar nuclei. From this research, we concluded that neurons were on stage of proliferation and migration in the embryo aged 40 days, then differentiated and migrated to form cortex  cerebellum and deep cerebellar nuclei at the age of 55 days, but the development of the cerebellum was not fully completed yet. 
Co-Authors . Ariana . Muttaqien Agnya Sinung Suminar Agus Purnomo Amelia Hana Amelia Hanna Amrullah Anindito Anak Agung Gede Sugianthara Aqiilah, Khairunnisa Ariana Ariana - Ariana Ariana Ariana Ariana Ariana Ariana Arvendi Rachma Jadi Arvendi Rahma Jadi Asyhari . Ayu Miftahul Khasanah Bambang Dwi Raharjo Bambang Dwirahardjo Bambang Sugito Bambang Sutrisno Budi Susilo Budiariati, Vista Cahya Yustisia Hasan, Cahya Yustisia Citra Ayu Pramesti Claude Mona Airin ClaudeM Mona Airin Daisynta Prima Aninditya Dewi Kania Musana Dewi Kania Musana Diah Tri Widayati Dondin Sajuthi Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwi Liliek Kusindarta Erdiansyah Rahmi Erni Sulistiawati Erwin . Erwin E Etriwati E Filphin Adolfin Amalo Gerarda Gita Puspitandaru Heni Pujiastuti Hery Widijanto Hery Wijayanto Hevi Wihadmadyatami Hevi Wihadmadyatami Iffah Sofana Intan Maharani Irene Linda Megawati Saputra Irma Padeta Jaswadi J Karina Mayang Sari Koeswinarning Sigit Koko Kurniawan Kustiati, Ulayatul Nemay Anggadewi Ndaong Nemay Anggadewi Ndaong, Nemay Anggadewi Niswah Nurul Fahma Nur Arofah Nuraini Rahmawati Nurhidayat . Nusaibah Nuraniyati Pradityo Yoga Wibowo PUDJI ASTUTI Rina Pratiwi Rini Widayanti Roza Azizah Primatika Sabrina Wahyu Wardhani Saragih, Golda Rani Sari, Yuslikha Khaniif Anggita Sitarina Widyarini Siti Khomariyah Soehartini Jatman Sumiyati Sunaryo Teguh Budipitojo Teguh Budipitojo Teguh Budipitojo Teguh Budipitojo Teguh Budipitojo Teguh Budipitojo Trini Susmiati Victor Lenda Vidya Irawan Vidya Irawan, Vidya Woro Danur Wendo Woro Danur Wendo Yosephine Nicolory Paula Yuda Heru Fibrianto Yulfia N Selan Yulfia Nelymalik Selan Yulfia Nelymalik Selan, Yulfia Nelymalik