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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 14 Documents
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Search results for , issue "Vol 31, No 2 (2013): DESEMBER" : 14 Documents clear
Morphological Characterization and genetic Diversity of Macrobrachium rosenbergii (De Man, 1879) Crossbreeding Result from Samas, Bone, and Sintetis Populations Trijoko .; Niken Satuti Nur Handayani; Anggun Feranisa
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1155.687 KB) | DOI: 10.22146/jsv.3812

Abstract

Abstract In Indonesia, research for the prime seed of Macrobrachium rosenbergii crossbreeding is rarely done. The aims of this study are to study F1's morphological character and genetic diversity from the result of crossbreeding between M.rosenbergii Samas, Bone, and Sintetis populations. In this study, genetic characterization was known by using PCR RAPD method utilizes three primers and morphological characterization. Data were analysed with UPGMA algorithm and Simple Matching coefficient that were presented in dendrogram. F1's coefficient heterosis was counted based on ratio from the average of cephalothorax and abdoment lenght and also ratio from the average of standard length of charapax and the average of abdomen length. The results showed that there was a very high genetic diversity in F1 population. The specific locus was found in individuals from Sintetis and Samsam populations.100% Polimorfism was found from F1's DNA amplification result, meanwhile monomorphism 50% was found from parent's DNA amplification result on OPA 20. The highest F1 heterosis was found in Samas and Sintetis genotipe.
Molecular Detection of Taura Syndrome Virus Infections in White Shrimp (Litopenaeus vannamei) and Giant Prawn (Macrobrachium rosenbergii) Fariha Willisiani; Nur Rohmah; Irma Nur Rahmawati; Nastiti Wijayanti
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (977.839 KB) | DOI: 10.22146/jsv.3813

Abstract

Abstract Giant prawn (Macrobrachium rosenbergii) and vaname shrimp (Litopenaeus vannamei) are types of shrimp that became excellent commodities in the fisheries sector. However, one of the obstacles in the vaname shrimp aquaculture is a disease caused by the infection of Taura Syndrome Virus (TSV). One of the consequence of raising the vaname shrimp in Indonesia is the possibility of spreading TSV infection in another shrimp species. TSV infection in giant prawns in Indonesia has not been reported. The aims of this study were: 1. To determine the resistance of giant prawns toward TSV infection and 2. To detect molecularly using RT-PCR technique the presence of viruses TSV on vaname shrimp or giant prawns infected with 3 different doses (0.05 ml; 0.10 ml and 0.15 ml) of TSV inoculum using a pair of specific primers for TSV 9992F (5'-AAG CTT GCG TAG ACA GCC-3') and 9195R (5'-TCA AGA ATG GCT TCC TGG-3'). The research results showed that vaname shrimp mortality infected by 0.05 ml; 0.10 ml and 0.15 ml TSV inoculums were 14.28%, 42.86%, and 57.14%, respectively. Whereas the giant prawns mortality that were infected using the same dose of TSV inoculums were 0%, 8.33%, and 8.33%, respectively. Positive result was detected molecularly only from haemolymph of vaname shrimp infected using 0.15 ml of TSV inoculum. On the other hand, positive results were detected in pleopod and the gill of vaname shrimp infected using 0.05 ml; 0.10 ml or 0.15 ml of TSV inoculums. In giant prawns, infection using 3 different doses of TSV inoculums causes negative result molecularly. Based on all of the facts, it can be concluded that, giant prawns has the higher resistance to TSV infection than that of vaname shrimp.
The Effect of Decis Insecticide on the Mortality and Gill Histological Structure of the Red Indigo "Cangkringan local strain" Wahyuni Wulandari; Sukiya .; Suhandoyo .
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1952.684 KB) | DOI: 10.22146/jsv.3814

Abstract

Abstract This study was aimed to determine the effects of Decis insecticide on the mortality, its safe level concentration against mortality, and the cytotoxic effects in the gills of the red tilapia strains of "localCangkringan" collected from Fish Seed Center, Cangkringan, Sleman, Yogyakarta. Eight hundreds fishweighing 0.5-1.0 g and 2-12 weeks old each were collected randomly and divided into 5 groups with Decis insecticide concentration of 0.27 ppm, 0.81 ppm, 1.00 ppm, 1.35 ppm, 2.4 ppm, respectively and a control group based on preliminary testing. Each treatment comprised three replicates, 10 replicates of each test fish. The observations of fish mortality to the test were done every hour - 24, for 96 hours of treatment, whereas mortality at safe levels test done every 4 weeks for 2 months of treatment. Data were analyzed using probit analysis to determine LC50 and LC50-96 hours-48 hours. The assay of 10% safe levels of LC50-48 hours is 0.13 ppm. Twofactorial analysis of variance was to determine the effect of concentration and duration of exposure to the Decis insecticide test fish mortality. Independent samples of T test analysis was applied to determine the effect of safe levels. The descriptive and qualitative analysis was done to determine the pathologic lesions on fish gills due to the cytotoxicity of Decis insecticide. Results of the present study showed that the variation of concentrations of Decis insecticide has significant effect on fish mortality (p <0,01). Decis insecticide concentration used for the LD50(96 hours)  was 1.21 ppm that was the highly toxic class to Nila. It caused severe cytotoxic efects to the gills  indicated by the presence of gills necrosis, hypertrophy, hyperplasia dan diffuse hemorrhages. The safety levelsof Decis concentration still caused mild histopathological lesions, such as focal to multifocal hemorrhages and congestion in the gills
Validation of the Spectrophotometry method on the concetration assessment of Enrofloxacine as the Animal Drug Ambarwati .; Novida Ariyani; Maria Fatima Palupi
Jurnal Sain Veteriner Vol 31, No 2 (2013): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.3815

Abstract

Abstract Validation for analysis of enrofloxacin injection method using spectrophotometry has been conducted. Enrofloksasin in a solution of NaOH (sodium hydroxide) 0.1 N, measured at the maximum wavelength (ë) 271 nm.The parameters of validation that were carried out are precision, accuracy, linearity, limit of detection and limit of quantitation. Precision showed good results with coefficien variation (CV) results smaller than that of CV Horwitz. Accuracy parameters calculated by the method of analysis to determine levels of analyte in a known active ingredient purity and yielded 0.449%. Linearity parameters showed good results with r = 0.9996. Limit of detection value was obtained for 0.757 μg / mL and limit of quantification of 2.522 μg / mL. The results of validation test of enrofloxacin injection analysis using spectrophotometric method can be used as a good method to provide a valid test of quality assurance veterinary drugs.

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