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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 14 Documents

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Search results for , issue "Vol 32, No 1 (2014): JUNI" : 14 Documents clear

Cytotoxicity Study of Red Fruit Extract (Pandanus conoideus Lam) as Anti-Goitrogenic Herbal Medicine in Sprague Dawley Rats Hastari wuryastuty; R. Wasito
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The use of herbal medicine (phytopharmaca) has increased manifold because of the safety. Red fruit extract is one of the supplements that has many advantages for health. The efficacy of pre-clinical trial using the rats induced by iodine deficient ration has proven that 1-2 ml of red fruit extract supplementation with/without KIO3 fortification for 30, 60 and 90 days has potency as anti-goitrogenic herbal medicine. For standarization, however, the extract red fruit has to be tested for the safety in the important organs like livers and kidneys. The research results have proven that in the normal Sprague Dawley rats, supplementation of 2 ml/day/rat of red fruit extract for 135 consecutive days has.no toxic effect in the livers and kidneys. In hyperplastic goiter Sprague Dawley rats induced by goitrogenic ration, the red fruit extract supplementation has to be in a combination with KIO3 to overcome iodine deficiency disorders and has no-toxic effect to the livers. Therefore, it can be concluded that based on pre-clinical studies using Sprague Dawley rats, the red fruit extract can be categorized as standardized anti-goitrogenic herbal medicine.

The Effect of Red Fruit's (Pandanus conoideus) Extract to Eritrocytes, Hemoglobin, PCV and TPP Concentrations of Mice (Mus musculus) Infected by Toxoplasma gondii Anis Dwi Utami; Dwi Priyowidodo
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Toxoplasmosis is a zoonotic parasites caused by Toxoplasma gondii. The red fruit’s extract contains tocopherol and betacarotene as antioxidant and it can repair the bodies immune system. This research was aimed to study the effect of red fruit’s extract to erithrocytes, hemoglobine, the values of PCV and TPP concentration of mice infected with T. gondii, so that the red fruit extract can be used as alternative herbal medicine for eliminating Toxoplasmosis. 15 of Balb/C mice, male, three months, 30 gr of body weight were used as experimental animals. The mice were then devided into three groups. Group I was a control, group II was tachyzoite infected and group III was tachyzoite infected and given red fruit’s extract. Group III given red fruit’s extract on first day with 0,53 g/kg BW/mice/day. Mice in group II and III were infected by injection Toxoplasma gondii tachyziote 1x103 a mice intraperitoneally. The blood sample was taken from five mice in each group at 7th and 12th day pass through cantus medialis. The blood was examinated in Clinical Pathology Laboratory, Faculty of Veterinary Medicine, Gadjah Mada University. Data were analyzed by using Factorial 2 x 3. The result showed no significant difference (P>0,05) in hemoglobin, and PCV’s value between groups I, II and III before and after infection of T. gondii’s tachyzoite. The TPP’s value between groups I, II and III before and after infection of T. gondii’s tachyzoite was significantly difference (P<0,05).

Ovulatory Follicular Dynamics After Estrus Synchronization using Prostaglandin F2a in Dairy Cows Prabowo Purwono Putro; Asmarani Kusumawati
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The study aimed to follow development of ovulatory follicular dynamics as well as plasma progesterone profile after estrus synchronization using PGF2 and GnRH. A total of 15 non-pregnant dairy cows, 4-5 years of age, healthy and reproductively sound were used in the present study. Treatment 1, given intramuscular injection of PGF2 25 mg (PGF2), treatment 2 PGF2 25 mg and GnRH 250 g 2 days later (PGF2-GnRH), and treatment 3 with GnRH 250 g (7 days prior to injection of PGF2), PGF2 25 mg and GnRH 250 g (2 days after injection of PGF2) (GnRH-PGF2a-GnRH) (the Ovsynch method). Transrectal ultrasonographic examination using real time, B-mode, with 7.5 MHz tranducer was performed everyday for 12 days to follow ovulatory follicular and luteal dynamics. Blood plasma was taken every day for progesterone determination using EIA technique. Data of follicular, luteal development and progesterone levels were tested using analysis of variance and correlation analysis. The animals showed estrus within 70.70 + 01.90 hours following PGF2 injection. Prostaglandin F2 induced corpus luteum regression, decreased in progesterone plasma levels, followed by ovulatory follicular development and eventually underwent ovulation. Administration of first GnRH increased corpus luteum size, enhanced its regression and decreased plasma progesterone levels, while the second administration induce better ovulatory follicular development. Rate of the corpus luteum regression, progesterone decrease and ovulatory follicular development following PGF2 injection for respective treatments 1, 2 and 3 were 2.53 + 0.24a, 2.73 + 0.36a and 3.53 + 0.28b mm/day; 1.39 + 0.14a, 1.35 + 0.18a dan 1.57 + 0.12b ng/ml/day; and 1.33 + 0.15a, 1.63 + 0.19b and 1.67 + 0.23b mm/day, respectively (P < 0.05). It can be concluded that PGF2 induced corpus luteum regression, decreased in progesterone plasma levels and ovulatory follicular development. Addition of GnRH increased corpus luteum size and plasma progesterone levels, after PGF2 injection corpus luteum regression and progesterone decrease became more prominent, while ovulatory folliculkar development occurred much better. .

The Effect of Thawing Temperature on Sperm Quality of Friesian Holstein Bulls Tri Utami; Tarsisius Considus Topianong
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The percentage of sperm motility and morphology are important criteria in evaluating the quality of sperm before it is used for artificial insemination (AI). This study was conducted to observe post thawing motility and abnormal morphology of spermatozoa Friesian Holstein (FH). The materials were used 10 straws of FH bulls in the form of 0.25 ml. A total of 10 straws then divided into two treatment groups of thawing in water at 37°C and water 8°C, respectively. An examination of the motility and morphology of spermatozoa abnormalities performed every two hours for two times. Calculating the percentage of sperm motility was done by calculating the percentage of spermatozoa moving forward in the field of view under a microscope with a magnification of 10x. the percentage of abnormal spermatozoa was assessed by William's stain. Spermatozoa morphology was observed by using a microscope magnification of 100x. Abnormalities of spermatozoa were calculated from a total of 200 spermatozoa, either normal or abnormal. At the same thawing time, the motility of FH cattle sperms post thawing in water temperature 37°C had a higher preference than that of post thawing in water temperature 8°C, although it was not significantly different (P > 0.05). Based on morphological aspects, frozen semen used in this study is within the tolerance limit for the total percentage of abnormal sperm morphology between 12% to 23% and normal morphology between 70% to 88%.

The Effect of Various Thawing Temperatures of Frozen Semen on the Success of Artificial Insemination of Beef Cattle Ida Arlita Wulandari; Surya Agus Prihatno
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The study about the effect of various thawing temperatures frozen semen on the success of artificial insemination of beef cattle had been conducted in Residency of Sleman Territory of Yogyakarta. The study used 59 heifers and cows that were oestrus (without oestrus stimulation) with body condition score (BCS) of 3 and 1,5-8 years age. They were inseminated using frozen semen of Simmental thawed in temperature of 37°C, 35°C (warm water) and 28-30°C (cold water). Effect of thawing temperatures was evaluated by non returnrate (NR). NR of thawing temperatures of 37°C, 35°C and 28-30°C were 63,16%, 55%, and 45%. Thawing temperature of 37°C increased the non returnrate compared to the thawing temperatures of 35°C and 28-30°C. Statistical analysis by Chi-square showed there were no differences between thawing temperatures of 37°C, 35°C and 28-30°C on the success of artificial insemination

Review of The Artificial Insemination Result Based on Anestrus Post Insemination in Bali Cattle Herds at The Regency of Sikka, East Nusa Tenggara Tarsisius Considus Topianong; Agung Budiyanto; Erif Maha Nugraha Setyawan
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Anestrus is one of indirect clinical signs of cattle pregnancy. Based on the present of sufficient progesterone on the blood, the cattle with pregnancy condition will not show the signs of estrus. The success of artificial insemination (AI) is the occurrence of fertilization followed by pregnancy and parturition. This involves a complex relationship between semen quality and oocytes, estrus detection, AI punctuality, inseminator and farmers ability. Artificial insemination should be performed at the relative optimum time to ovulation. Estrus detection is an important factor to determine the time of AI. Estrus Detection of Bali cattles of the farmer is often difficult observed or not observed, especially in semi-intensive system maintenance. From this review it can be seen that there is the different proportion of inseminators on the incidence of estrus after AI. However, determination of the role of inseminator on the success of AI and then the other factors that affect the success of the AI should be under ideal conditions. Straw from the different bulls breed gave the same of AI result. The estimate of pregnancy rate of 80 cows after AI is 55 %. Estrus detection on 21st days after AI can be used as a detection method for early diagnosis of pregnancy, especially for the farmers who did not have any ability or authority to perform medical acts of reproduction which is only owned by a veterinarian. Observation, recording and evaluation of the implementation of the AI should be made until the end of pregnancy followed by the process of parturition.

The Influence of Kebar Grass Infuse to Mice (Mus musculus) Spermatogenesis Paula Nency Lefaan
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study was aim to examine the effect of grass infusion kebar (Biophytum petersianum) to increase the activity of spermatogenesis in male mice. This research was conducted at the Laboratory of Botany University Discourse Kristen Duta Yogyakarta, Laboratory of Experimental Animal Care Unit and Unit III LPPT Gadjah Mada University in April 2009 to May 2009 using Swiss strain male mice aged 2.5 months as many as 25 tails, which are grouped in 5 groups treated with different concentrations, 0% (control), 1%, 3%, 5%, and 10% for 30 days, 2 times a day at a dose of 0.5 ml each time. Results of TLC method proves that these plants contain three types of chemical compounds that could potentially impact the process of spermatogenesis, ie, saponins, flavonoids and tannins. The analysis showed an increase in activity with increasing concentrations of spermatogenesis up to a concentration of 5% (spermatogonia: 226, primary spermatocytes: 227, secondary spermatocytes: 377, spermatids: 302) with a significant value of 0.432. Yet, at a concentration of 10% actually decreased spermatogenesis activity due to negative feedback. Conclusion: Infuse kebar grass proved to increase the activity of spermatogenesis, but at concentrations that are too high can cause a decrease in spermatogenesis activity.

Total Plate Count of Milk from Dairy Cooperatives in Yogyakarta and East Java Monica Septiani; Yatri Drastini
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Most of the local populations in Indonesia, especially in Sleman, Yogyakarta and Tulungagung, East Java consume cow milk. Cow milk is more widely consumed than that of horse or goat because of the people habit and the raw milk is more easily found in the market. The majority of people consume milk without knowing the number of bacteria contained and neither the presence nor absence of harmful bacteria in the milk consumed. This study aimed to identify the total number of bacteria in several dairy cooperatives in Sleman, Yogyakarta and Tulungagung, East Java. Milk samples were taken from two tanks in Koperasi Sarono Makmur (KSM), three tanks (were taken four times, once a week) in Koperasi Warga Mulya (KWM) Sleman, Yogyakarta, and two tanks (twice in the morning and once in the evening) in Koperasi Unit Tani Ternak (KUTT) Tulungagung, East Java. Milk from each tank were taken 500 mL and put into the cooler that was given ice cubes. Then the samples to be tested for total bacteria from each tank with plate count agar medium. The number of samples was 19. Milk samples were statistically analyzed with one way ANOVA. The results showed that the total number of bacteria of milk of KSM is 1.0 x 106 CFU/mL, KWM 3.6 x 106 CFU/mL, whereas cow's milk KUTT 4.0 x 106 CFU/mL. The average total number of bacteria of KWM and KUTT exceeds the requirements of the Indonesian National Standard (SNI) 01-3141-1998 (1,0 x 106 CFU/mL).

Prevalence of Toxoplasmosis in Sheep Slaughtered in Ngampilan Slaughterhouse Yogyakarta Using CATT Method Rika Yuniar Siregar; Yuswandi .
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The research was conducted to find out the prevalence of toxoplasmosis in sheeps which were slaughtered in Ngampilan Slaughterhouse Yogyakarta using card agglutination test (CATT) method. Blood samples were collected from 50 sheeps. The blood samples were then centrifuged at 5000 rpm for 5 minutes in 4°C in order to obtain the sera of the sheeps. After that, each serum in the amounts of 10 µl was tested using CATT method. The positive CATT test resulted in the green background with red aggregate in the middle, whereas the negative CATT test resulted in the uniform brown discoloration. In conclusion, about 72% of sheeps slaughtered in Ngampilan Slaughterhouse Yogyakarta was positively infected toxoplasma. Toxoplasmosis prevalences for ewes, rams, sheeps and lambs are 79 %, 59 %, 72 % and 67 %, respectively.

Immunocytochemical Study on Blood and Organ Suspension of Tiger Grouper (Epinephelus fuscoguttatus) Infected with Field Isolate of Viral Nervous Necrosis Artanti Tri Lestari; Putu Eka Sudaryatma
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

One potential marine cultures that have been developed and started to show the international market is grouper. Grouper culture can not be separated from factors that can affect disease and thwart cultivation. One of the diseases that has been reported by researchers is viral nervous necrosis (VNN) causing mass mortality in fish, especially grouper larvae and juvenile stadia. Laboratory of Balai KIPM kelas l Denpasar develop rapid diagnostic techniques, precise and accurate test using immunocytochemistry of blood and organs as one of the initial inspection. Tiger grouper sized 150-300 g as much as 50 and acclimatized, then 10 fishes used as controls, 40 fishes were injected with inoculum VNN 101.5 reared without water replacement cycle for ten days. Clinical observation and organ sampling performed 12 hours post-infection and consecutive every 12 hours. Blood samples and organs were collected for immunocytochemical (streptavidin-biotin) and a confirmatory test using RT - PCR using kit IQ -2000 VNN. Immunocytochemistry and RT-PCR showed positive results against VNN blood smears and suspensions organs of grouper fish with 24 hours post-infection . Based on the test results, the immunocytochemistry test on the blood and organ suspensions can be used as a detection technique VNN which is rapid, precise and accurate.

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