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All Journal Hemera Zoa Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal Kedokteran Hewan

Surya Agus Prihatno

Departemen Reproduksi Dan Obstetri Fakultas Kedokteran Hewan Universitas Gadjah Mada

Author-ID : 2895618

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary Other

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PCS-10 The Features of Seminiferous Tubule Cells in Rat Testicular Dysfunction Induce by Secretome Based On Cytoskeletal Protein Profile Surya Agus Prihatno; Irma Padeta; Arinda Devi Larasati; Betty Sundari; Annisa Hidayati; Yuda Heru Fibrianto; Teguh Budipitojo
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1626.268 KB)

Secretome is a factor found in the stem cell culture medium. This factor may repair the tissues of the organs that were damaged by various degenerative disorders1. A previous study has reported that secretome derived from the fetal human umbilical cord mesenchymal stem cell (HUC-MSC) may be an effective regenerative agent for β-cell pancreatic regeneration in Type 1 diabetes mellitus and for skin regeneration in incisional and burn wound healing.The administration of cisplatin as a chemotherapeutic agent is limited because it causes side effects, such as reproductive toxicity and progresses to testicular dysfunction2. As a degenerative disorder, testicular dysfunction is characterized by failure in the synthesis of reproductive hormones and spermatogenesis. Cisplatin-induced testicular cell damage would promote vimentin and cytokeratin localization in the spermatogenic, Sertoli, and Leydig cells.Studies on the use of secretome in promoting the recovery of various degenerative disorders and its effects on the spermatogenesis of rats with cisplatin-induced testicular dysfunction have not been conducted. The aim of this stud was to investigate the effect of secretome derived from HUC-MSC on cisplatin-induced testicular dysfunction in rats.

PENGARUH PEMBERIAN PROSTAGLANDIN F-2a DAN GONADOTROPHIN RELEASING HORMON TERHADAP ANGKA KEBUNTINGAN PADA SAPI PERAH YANG MENGALAMI KASUS KAWIN BERULANG Surya Agus Prihatno; Sri Gustari
Jurnal Sain Veteriner Vol 21, No 2 (2003): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Pengaruh Pemberian Prostaglandin F-2 alpha dan Methilergometrin Terhadap Timbulnya estrus setelah Beranak Pada Sapi Perah Surya Agus Prihatno
Jurnal Sain Veteriner Vol 21, No 1 (2003): JULI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Study of Repeat Breeder of Dairy Cows in Farmer Level Surya Agus Prihatno; Asmarani Kusumawati; Ni Wayan Kurniani Karja; Bambang Sumiarto
Jurnal Sain Veteriner Vol 30, No 2 (2012): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

One of the important reproductive disorders in dairy cows was repeat breeding. Economic losses was quite high, due to the low reproductive efficiency and productivity. Breeder is one of the risk faktors on the incidence of repeat breeding. The aims of this research were to determine the incidence of repeat breeding cases in dairy cattle at Special Regency of Yogyakarta (DIY) and many factors from the farmers and dairy cattle aspects that caused the cases. In this study, 401 traditional farmers at DIY were used. The method of data collection was done by interviewing the farmers and direct observation to the dairy cattle. The data consist of farmer education level, length period of farming experience, stall condition, length distance from inseminator, estrous detection ability,knowledge of estrous, estrous observation by farmer, stall cleanliness, cattle cleanlines, and squeezing time.Data were collected on a descriptive analysis of each variable studied. Analysis used logistic regression models to analyze the faktors that influence the incidence of repeat breeding. The sensitivity and specificity of the models were analyzed by Lemeshow goodness of fit test.The result of this study showed that the prevalence of repeat breeding in dairy cattle from farmer aspect was 29.4%. The factor from farmer aspect which affected repeat breeding cases was estrus detection once per day(coefficient regression 4.09563, OR=60,1), detection of estrus twice daily (+ 2.92553, OR=18.6) , disordersof pregnancy (+2.22630, OR= 9.3), dirty environment (+0.91889, OR=2,5), worst drainage (+2.58072, OR=13,2), and education (+1.31295, OR=3,7). It was concluded that the prevalence of repeat breeding at DIY as big as 29.4%. Risk factors affecting the repeat breeding were estrus detection once per day, detection of estrus twice a day, dirty environment, worst drainage, and education. Keywords : prevalency, repeat breeding, dairy cattle, logistic regression, odds ratio

The Effect of Various Thawing Temperatures of Frozen Semen on the Success of Artificial Insemination of Beef Cattle Ida Arlita Wulandari; Surya Agus Prihatno
Jurnal Sain Veteriner Vol 32, No 1 (2014): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The study about the effect of various thawing temperatures frozen semen on the success of artificial insemination of beef cattle had been conducted in Residency of Sleman Territory of Yogyakarta. The study used 59 heifers and cows that were oestrus (without oestrus stimulation) with body condition score (BCS) of 3 and 1,5-8 years age. They were inseminated using frozen semen of Simmental thawed in temperature of 37°C, 35°C (warm water) and 28-30°C (cold water). Effect of thawing temperatures was evaluated by non returnrate (NR). NR of thawing temperatures of 37°C, 35°C and 28-30°C were 63,16%, 55%, and 45%. Thawing temperature of 37°C increased the non returnrate compared to the thawing temperatures of 35°C and 28-30°C. Statistical analysis by Chi-square showed there were no differences between thawing temperatures of 37°C, 35°C and 28-30°C on the success of artificial insemination

Analisis Epidemiologi Kasus Hipofungsi Ovarium pada Sapi Potong di Kabupaten Jepara Aldi Salman; Surya Agus Prihatno; Bambang Sumiarto
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.56788

Hipofungsi ovarium menjadi kasus gangguan reproduksi yang memiliki angka kejadian paling tinggi di Jawa Tengah, dengan kerugian peternak karena panjangnya Calving Interval dan biaya pengobatan yang tinggi. Kualitas pakan seringkali dianggap menjadi menjadi penyebab hipofungsi ovarium, tetapi juga terdapat faktor lain yang mengakibatkan munculnya penyakit. Penelitian bertujuan untuk mengetahui prevalensi dan faktor risiko hipofungsi ovarium pada sapi potong, serta model untuk memprediksi penyakit hipofungsi ovarium di Kabupaten Jepara. Sapi betina produktif sebanyak 304 ekor sampel dari 176 peternak dipilih secara formal random sampling pada 14 desa di 7 kecamatan dengan tahapan ganda. Dilakukan anamnesis pada peternak dan pemeriksaan sapi secara per rektal untuk mengetahui status reproduksi, serta kuesioner untuk tingkat peternakan dan individu ternak. Hasil penelitian menunjukkan prevalensi hipofungsi ovarium 8,88% dan faktor risiko yang mempunyai hubungan adalah frekuensi pakan tambahan/PKTB (OR=12,77) dan pakan utama/PKUT (OR=9,59). Variabel yang menurunkan hipofungsi ovarium adalah jenis ternak/JNT (OR=0,37), kepemilikan ternak satu/PUNYA1 (OR=0,34), umur ternak/UMT (OR=0,32), status laktasi/STLAK (OR=0,07) dan umur sapih/SAPH (OR=0,027). Model persamaan pada tingkat ternak adalah Ln P/1-P = 5,709 - 3,198xSAPH - 1,825xSTLAK - 0,992xJNTR. Model persamaan pada tingkat peternakan adalah Ln P/1-P = 1,213 + 1,813xPKUT + 1,736xPKTB - 1,331xPUNYA1

Pengaruh Pemberian GnRH pada Sapi Potong yang Mengalami Kawin Berulang (Repeat Breeding) Surya Agus Prihatno; Sri Gustari; Asmarani Kusumawati; Agung Budiyanto; Erif Maha Nugraha Setyawan; Yosua Kristian Adi
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.62761

Repeat breeding is a condition in which cows have normal estrous cycles and have been mated 3 times or more but are not pregnant. The incidence of repeat breeding in beef cattle can be detrimental to breeders in terms of time, that the calving period becomes longer so that the cows cannot give birth to calves once a year. Various ways to deal with repeat breeding have been done, but the results are not optimal. This study was conducted to determine the effect of Gonadotrophin Releasing Hormone (GnRH) in cows with repeat breeding. A total of fifteen beef cattle with repeat breeding were used in this study. The cows were divided into 3 groups of 5 each. Cows that were estrus in the first group (as a control group) were only performed artificial insemination (AI) without GnRH injection. In the second and third groups, after AI, they were injected with GnRH at AI time and 3-4 days after AI with a dose of 5 ml (Fertagyl®, 500 µg gonadorelin) intramuscularly. A pregnancy examination was carried out three months later and the results were recorded. The data obtained were analyzed descriptively and quantitatively using Chi-Square statistical analysis. The results of the analysis showed that there were differences in the pregnancy rate in the first group (control) with the second cow group and the third cow group, which were 20%, 80%, and 60% respectively. Based on statistical analysis, there was a significant difference in pregnancy rate (P <0.05) between the three groups. It was concluded that GnRH administration could increase the pregnancy rate in beef cattle that had repeat breeding.

Identifikasi Mikroba dari Lendir Estrus dan Deteksi Kebuntingan Sapi Kawin Berulang di Sleman, Yogyakarta Surya Agus Prihatno; Abdul Samik; Dea Indriani Astuti; Muhammad Agil; Usamah Afiff; Anriansyah Renggaman; Dedi Rahmat Setiadi; Yosua Kristian Adi
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.70916

Repeat breeding is condition of cows that have normal or near normal estrous cycles but fail to become pregnant after several insemination. In this study, we investigated the microbes in the cervical and vaginal mucus of beef cattle and dairy cows that experience repeat breeding and detected the pregnancy after the third or more artificial insemination. A total of 14 beef cattle and 6 dairy cows that experience repeat breeding in the cattle herd in the Yogyakarta region of Indonesia were used as samples in this study. Cervical and vaginal mucus samples were collected using plastic sheet when the cow was re-estrus. The samples were put into the 5 ml Brain Heart Infusion Broth for bacterial isolation and identification. Pregnancy examination was carried out on day 45 after artificial insemination using ultrasound method. Bacteria that could be isolated and identified from cervical mucus and vaginal mucus of beef cattle and dairy cows included Bacillus sp., Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas sp. Some cows detected positive in pregnancy examination even though the bacteria were presence in the cervical and/or vaginal mucus. There was various composition of bacteria found in the cervical mucus and vaginal mucus, of beef cattle and dairy cows with repeat breeding in livestock groups in Yogyakarta. The presence of bacterial in the cervical and vaginal mucus during estrus was not always become the causes of failed pregnancy.

WAKTU OVULASI DAN ANGKA KEBUNTINGAN PADA SAPI PERAH YANG MENGALAMI KAWIN BERULANG SETELAH PEMBERIAN GnRH, VITAMIN ADE DAN INFUSI IODIUM POVIDON Surya Agus Prihatno
Jurnal Sain Veteriner Vol 40, No 1 (2022): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.73480

Waktu ovulasi dan ANGKA KEBUNTINGAN PADA SAPI PERAH YANG MENGALAMI KAWIN BERULANG SETELAH PEMBERIAN GnRH, VITAMIN ADE DAN INFUSI IODIUM POVIDON Intisari Angka kebuntingan pada sapi perah yang mengalami kawin berulang sangat rendah. Dampak kerugian secara ekonomi bagi peternak sangat nyata disebabkan rendahnya efisiensi reproduksi dan produktivitas. Penelitian ini bertujuan untuk mengetahui waktu ovulasi dan angka kebuntingan pada sapi perah yang mengalami kawin berulang setelah pemberian GnRH, vitamin ADE dan infusi iodium povidon. Sebanyak 24 ekor sapi perah FH betina pernah beranak, mempunyai korpus luteum, tidak bunting, umur 3-6 tahun, mengalami kawin berulang, secara klinis sehat dan siklus reproduksi normal digunakan dalam penelitian ini. Sapi dibagi 4 kelompok terdiri dari 6 ekor per kelompok perlakuan. Kelompok P1 sebagai kontrol, kelompok P2 diberi GnRH dosis 250 µg per ekor (Gonadorelin, FertagylTM, Intervet, International B.V., Boxmer, Holland), kelompok P3 diberi vitamin ADE dengan dosis 5 ml dan infusi iodium povidon 1% secara intra uterus. Kelompok P4 diberi vitamin ADE, infusi iodium povidon 1% intra uterus, dan GnRH. Singkonisasi estrus menggunakan Prostaglandin F2α dengan tujuan menyeragamkan waktu timbulnya estrus. Semua sapi estrus dikawinkan secara insiminasi buatan (IB). Pemeriksaan waktu ovulasi secara perrektal kurang dari 12 jam, 12 sampai 24 jam dan lebih dari 24 jam setelah estrus berakhir dan angka kebuntingan menggunakan ultrasonography (USG) pada 30-45 hari dan palpasi rektal pada 60-90 hari setelah inseminasi. Analisa hasil menggunakan deskripsi dan Kruskal-Wallis test. Hasil penelitian menunjukkan waktu ovulasi kurang dari 12 jam, 12 sampai 24 jam dan lebih dari 24 jam masing -masing P1 ( 16.7%, 66,7%, dan 16,7 %), P2 ( 66,7%, 33,3%), P3 (33,3%, 66,7%), P4 (83,3%, 16,7%). Angka kebuntingan pada kelompok kontrol dan perlakuan masing-masing sebesar P1 (kontrol) 16,67%, P2 (diberi GnRH saat IB) 66,67%, P3 (diberi vitamin ADE dan Infusi iodium povidon 1%) 50% dan P4 (diberi vitamin ADE, Infusi iodium povidon 1%, dan GnRH) 100%. Ketepatan waktu ovulasi dan tingkat kebuntingan yang tertinggi dihasilkan oleh kelompok I yang berbeda secara signifikan (P<0,05) dengan kelompok kontrol. Disimpulkan pemberian GnRH, vitamin ADE dan infusi iodium povidon pada sapi perah yang mengalami kawin berulang mampu mempercepat waktu ovulasi dan meningkatkan angka kebuntingan. Kata kunci : GnRH, iodium povidon, kawin berulang, kebuntingan, sapi perah

Kondisi Ovarium dan Profil Hormon Progesteron pada Sapi Perah yang Mengalami Kawin Ulang (Repeat Breeder) Surya Agus Prihatno
Buletin Peternakan Vol 23, No 1 (1999): Buletin Peternakan Vol. 23 (1) Februari 1999
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v23i1.1652

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Co-Authors Abdul Samik Afiff , Usamah Agung Budiyanto Aldi Salman Annisa Hidayati Anriansyah Renggaman Arinda Devi Larasati Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Betty Sundari Dea Indriani Astuti Dedi Rahmat Setiadi Erif Maha Nugraha Setyawan Ida Arlita Wulandari Irma Padeta MUHAMMAD AGIL Ni Wayan Kurniani Karja Sri Gustari Teguh Budipitojo Yosua Kristian Adi Yosua Kristian Adi Yuda Heru Fibrianto

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