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INDONESIA
Jurnal Sain Veteriner
Published by Universitas Gadjah Mada
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Veterinary
Arjuna Subject : -
Articles 14 Documents
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Search results for , issue "Vol 32, No 2 (2014): DESEMBER" : 14 Documents clear
The Effects of Hymeniacidon sp. Sponge Extract to The Level of Parasitemia Trypanosoma evansi in BALB/c Mice (Mus musculus L.) Maya Ekaningtias
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (785.694 KB) | DOI: 10.22146/jsv.6557

Abstract

Trypanosoma evansi is a blood protozoa which has infected many livestock like cow, horse, and buffalo and caused disease so called surra. Disease medication of surra depends on the latest invention of trypanocidal medicine, but must of synthetic medicine has become resistance. Therefore, another alternative has been developed using natural materials. Sponge is a kind of oceanic organism which well known for producing secondary metabolite which has biology activity. One of them is Hymeniacidon sp. which known containingterpenoid bioactive compound. The purpose of this research is to study the effect of Hymeniacidon sp. sponge extract to the level of parasitemia T. evansi in Balb/C mice (Mus musculus L.) and to find out the most optimum Hymeniacidon sp. sponge extract to prevent T. evansi growth. This research has used 40 male Balb/C mice, age 2-3 months, weight ± 23-30 grams. The research subject was divided in to 8 groups (each group contain 5 mice).The classification consists of negative control DMSO, negative control of sponge extract, positive control which is infected by T. evansi and 5 groups treatment T. evansi accompanied with sponge extract given. Sponge extract concentration which was used were 50 mg/kg of BB, 100 mg/kg of BB, 200 mg/kg of BB, 400 mg/kg of BB, and800 mg/kg of BB, respectively. Mice were injected intraperitoneally with 0,2 ml of blood containing 103 of T. evansi. Sponge extract was given orally at 24 hours after T.evansi infection and conducted during 4 days. Then, film blood smear is made, observed by microscope and level of parasitemia T.evansi in blood was calculated.Result of this research indicated that Hymeniacidon sp. kloroform sponge extract concentration variation significantly decrease to the level of parasitemia T. evansi. The most effective concentration is 400 mg/kg of BB with reactivity equal to 98,71% on the fourth days research.
Histopathological Features of Duodenal Coccidiosis in the Probiotic Broiler Chickens Aven B Oematan; Damai Kusumaningrum
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1222.143 KB) | DOI: 10.22146/jsv.6558

Abstract

This aim of research was to know histopathology lesions of broiler duodenum in chicken coccidiosis with probiotic administrasion. Sixty six DOC of broiler, strain Arbor acress, male sex were divided into 5 groups of twelve each. Chickens in Groups I, II, III, IV and V were given standard food, standard food+probiotic, standard food+1000 oocysts, standard food+probiotic+1000 oocysts and standard food+probiotic+ 3000 oocysts,respectively. Probiotic was given starting at the age of 8 days by sprayed in food with the dose 2 ml/ 250 ml water/ 1 kg food. At the age of 38 days, chickens were infected orally by given 1000 oocysts for group III and IV, and 3000 oocysts for group V. Group I and II were euthanazed at the age of 39 days, group IIIA, IVA, VA at 5 days postinfection, group IIIB, IVB, VB at 6 days post infection, and group IIIC, IVC, VC at 7 days post infection. Histopathologic lesions of the duodenums were analyzed with qualitative analyze. Results showed that infection without probiotic area were found histopathologic changes of duodenum in the form of congestion, hemorrhages, schizonts and macrogametes representing of Eimeria sp stadium which caused coccidiosis. Treatment infection with probiotic were found histopathologic changes in the duodenum, such as congestion,hemorrhages, necrotic epithelial cells, and also schizonts, microgametes and macrogametes representing of Eimeria sp stadium which caused coccidiosis. Probiotic in the present study did not have any effect for histopathologic changes in the duodenum in the broiler chickens infected coccidiosis.
Total Fat, Calcium (Ca), Iron (Fe) and Zinc (Zn) Contents During Ecdysis process in the Crabs (Scylla serrata, Forsskal) Sri Swasthikawati; Rarastoeti Pratiwi; Trijoko .
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (553.957 KB) | DOI: 10.22146/jsv.6559

Abstract

Crab is one of the most abundant marine animals in Indonesia. Crabs not only rich of nutrient, but also have commercial value. Today, there is an innovation in crab aquaculture use ecdysis mechanism, known as soft shell crab. However, the limited studies about biochemical and nutritional composition of this crab remain to be explored. The aims of this research were to understand total fat, calcium, iron, and zinc contents of crab (Scyllaserrata) during ecdysis process until being soft shell crab comparing to the hard shell crab from the same species. This research was done using crab Scylla serrata, male, weighing ± 150-180 gr, completely randomized design with four variations of treatment, during 8 days, 16 days, soft shell crab, and control, repeated 3 times. Samples from each treatment were grown and were prepared for the chemical analysis. Total fat was measured usingSoxhlet method with chloroform and methanol (1:2, v/v) as solvent. Atomic absorption spectrometer was used to measure calcium, iron, and zinc contents. Then, the result were analyzed using one way Anova and Duncan 5 %. The results showed that the total fat, calcium and zinc contents of soft shell crab Scylla serrata were higher and significantly different with three other treatments. It could be concluded that the ecdysis process, raised total fat, calcium and zinc contents of crab Scylla serrata. The total fat, calcium, and zinc contents of soft shell crab Scylla serrata was higher than that of hard shell crab from the same species.
North Kalimantan Is Free from EMS (Early Mortality Syndrome) in the Shrimps Sab Lestiawan; Nugroho Sasongko Jati; Hermas Wiro
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1573.275 KB) | DOI: 10.22146/jsv.6560

Abstract

The acute hepatopancreatic necrosis syndrome (AHPNS) or also known worldwide as early mortility syndrome (EMS) is very dangerous for the shrimps industry since it really strikes the shrimps by larvae size. The EMS attacks shrimps at 20 to 30 days of age and at 40 days of age as well. All the shrimps that are being infected will be dead righ away. The EMS has caused the significant economic losses in the shrimps industry due to the mass mortality of the shrimps in Vietnam (in the years of 2010- 2012). It also occurred ad spread around China (2009), Vietnam (2010), East Malaysia (2010), East of Thailand (2012) until approaching the edge of Cambodia (2013). It has been reported that the EMS is likely due to Vibrio parahaemolitycus that are infected by certain virus (phage), and then the bacteria releasing very toxic substances (toxin). Efforts has been being done by the Indonesian government, especially in the Fish Quarantine Office, Kls II, Tarakan in the form of prohibition of entry of live and/or frozen shrimps importing from the EMS-outbreak country. Besides that, monitoring and laboratory works has also been performed routinely and periodically. The results proves thatNorth Kalimantan is free of AHPNS (EMS).

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