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Ayyub Harly Nurung
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Editor's Address: 3rd Floor Laboratory Faculty of Pharmacy, 2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia
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INDONESIA
Journal Microbiology Science
Published by Universitas Muslim Indonesia
ISSN : 2808392X     EISSN : 28083911     DOI : https://doi.org/10.56711/jms
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Journal Microbiology Sciences (J. Mic. Sciences) receives writing in the form of research results, scientific studies and brief descriptions relating to Microbiology, such as Molecular and Microbial Epidemiology Clinical Microbiology Medical Microbiology Pharmaceutical Biotechnology and others related to Microbiology Sciencee
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 5 Documents
 1 
Search results for , issue "Vol 3, No 1 (2023)" : 5 Documents clear
IDENTIFICATION OF ACTIVE CHEMICAL COMPONENTS OF ETHANOL EXTRACT OF PAINTED NETTLE LEAVES (COLEUS SCUTELLARIOIDES L. BENTH) AGAINST GASTROINTESTINAL INFECTION BACTERIA USING TLCBIOAUTOGRAPHY AND AGAR DIFFUSION METHOD Junaedi, Fhadillah; Kosman, Rachmat; Herwin, Herwin
Journal Microbiology Science Vol 3, No 1 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.004 KB) | DOI: 10.56711/jms.v3i1.869

Abstract

Infectious diseases are one of the public health problems caused by the transmission and multiplication of microorganisms. The research on the antibacterial activity of painted nettle leaves (Coleus scutellarioides L. Benth) extracts was carriedout to examine the activity and chemical components that provide inhibition against gastrointestinal infection bacteria. The extraction of painted nettles leaves performed maceration methd using a solvent of 96%. The extraction results were examined to determine the antibacterial activity at 1% concentration of the test bacteria: Escherichia coli, Salmonella thypi, and Shigella dysenteriae. The results showed that 96% ethanol extract of painted nettle leaves inhibited Escherichia coli, Salmonella thypi, and Shigella dysenteriae. The research conducted using agar diffusion indicated that painted nettleleaf extract showed activity against bacteria and had highly strong potential with diameters of 6-20 mm. The results of Thin Layer Chromatography (TLC) Bioautography test of ethanol extract of painted nettle leaves using chloroform:methanol eluent (3:1) showed inhibition of Escherichia coli, Shigella dysenteriae, and Salmonella thypi at Rf values of 0.90, 0.80, 0.56. Therefore, the identification test confirmed that the chemical componentsproviding antibacterial are from flavonoids, phenols, terpenoids, alkaloids, and steroidsKey words: Identification of chemical components, painted nettle leaves, gastrointestinal bacteria, TLC-Bioautography and Agar diffusion
IDENTIFICATION OF ENDOPYTIC FUNGI COMPOUND ON WHITE WEED LEAVES (Ageratum Conyzoides L.) CONTAINING THE POTENTIAL TO PRODUCE ANTIBIOTICS BY TLC-BIOAUTOGRAPHY. Rofika, Evi; Fitriana, Fitriana; Herwin, Herwin
Journal Microbiology Science Vol 3, No 1 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.835 KB) | DOI: 10.56711/jms.v3i1.894

Abstract

White weed (Ageratum conyzoides L.) is known to have antibacterial elements containing chemical compound such as saponin and flavonoids. This study aimed to examine the antibiotic activity of endophytic fungi isolates of white weed leaves by the TLC-Bioautography method. The results of endophytic fungi isolation of white weed leaves obtained 10 isolates consisting of IFDP 1, IFDP 2, IFDP 3, IFDP 4, IFDP 5, IFDP 6, IFDP 7, IFDP 8, IFDP 9, and IFDP 10. The results of the macroscopic examination of the ten isolates of endophytic fungi found different characteristics. The results of the screening using 9 samples obtained isolates that found activity showing high inhibitory power, such as IFDP 1, IFDP 2, and IFDP 4 isolates. Isolates of IFDP 1, IFDP 2 and IFDP 4 were fermented on MYB medium for 14 days. Then filtering and evaporation were carried out to produce an extract. Isolate fermentate extracts were identified using Thin Layer Chromatography with chloroform eluent: methanol (4:2). The antibiotic activity test was done using TLC-Bioautography method, and obtained an Rf value in isolate 1, at Rf 0.74 on Vibrio cholerae. Isolate 2 resulted rf value of 0.67 on Salmonella thypi, Vibrio cholerae, Eschericia coli, disentriae, Staphylococcus epidermis. Isolate 4 generated an Rf value of 0.74 on Pseudomonas aeruginosa and Vibrio cholerae. While the group of active chemical components contained in the endophytic fungi isolates of white weed leaves was lavonoids by using AlCl3 reagents, AICI3 reagent alkaloids, and Sulfuric acid for saponin identification
ISOLATION OF ENDOPHYTIC FUNGI FROM PATCHOULI LEAVES (POGOSTEMON CABLIN BENTH) AS ANTIBACTERIAL AGAINST PATHOGENIC BACTERIA BY BIOAUTOGRAPHY AND AGAR DIFFUSION Arif, Sitti Nur haerani
Journal Microbiology Science Vol 3, No 1 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.95 KB) | DOI: 10.56711/jms.v3i1.910

Abstract

Patchouli (Pogostemon cablin Benth) is a plant used as a substance in the pharmaceutical industry such as the manufacture of anti-inflammatory, antifungal, anti-insect and anti-inflammatory drugs. This study aimed to obtain endophytic fungi isolates having the potential as antibacterial by using TLC- Bioautography and agar diffusion methods. This research was conducted by isolating the endophytic fungi from Patchouli leaves and after that, purification and macroscopic tests were carried out and ten isolates were obtained pure. The results of the isolate screening test with codes IFDN 1, IFDN 3, IFDN 4, IFDN 6, IFDN 7, IFDN 8 and IFDN 9, showed the highest inhibition. Furthermore, the antibacterial activity was assayed by TLC-Bioautography. The results showed active spots (Rf 3 = 0.54; Rf 4 = 0.61; Rf 6 = 0.58; Rf 7 = 0.45; Rf 8 = 0.54; Rf 9 =0.47) against Eschericia coli, Staphylococcus aureus and Staphylococcus epidemics. The results of the antibacterial activity test by diffusion method obtained an inhibition zone for each bacterium, namely Staphylococcus aureus of 8.66 mm, Staphylococcus epidermidis of 8.38 mm, and Eschericia coli of12.23 mm.Key Words: Agar diffusion; Antibacterial; Endophytic fungi; skin infections; TLC Bioautograph.          
ANTIBACTERIAL ACTIVITY TEST OF ETHANOL EXTRACT OF TEMBELEKAN LEAVES (Lantana Camara L) AGAINST BACTERIA THAT CAUSE SKIN INFECTIONS USING AGAR DIFFUSION METHODS AND TLC-BIOAUTOGRAPHY Sulfiana, Sulfiana
Journal Microbiology Science Vol 3, No 1 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.905 KB) | DOI: 10.56711/jms.v3i1.922

Abstract

Tembelekan with the scientific name Lantana camara Linn which is used for generations in medicine that utilizes leaves to treat skin diseases such as tinea versicolor, ringworm and ringworm. Skin infection is a disease that attacks the surface of the body, and is caused by various causes such as bacteria, viruses, and fungi. In this study, the antibacterial activity of the ethanolic extract of the tembelekan leaf (Lantana camara L) was tested using the agar diffusion method and TLC- Bioautography against several test bacteria including Propionibacterium acnes, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis. The results of the screening test for ethanol extract of tembelekan leaves (Lantana camara L) at a concentration of 0.5% can inhibit and kill the growth of the test bacteria. Then proceed with testing the antibacterial activity using the agar diffusion method. The result is that the diameter of the largest inhibition zone of the ethanolic extract of tembelekan leaf (Lantana camara L) was obtained at a concentration of 8%, namely Propionibacterium acnes (15.57 mm), Pseudomonas aeruginosa (17.82 mm), Staphylococcus aureus (16.14 mm). , Staphylococcus epidermidis (15.2 mm). The TLC results of the ethanolic extract of tembelekan (Lantana camara L) leaves with eluent n hexane: ethyl acetate (7: 4) viewed under UV light at 254 nm and 366 nm, 6 stains were seen, each with an Rf value of Rf1 0.90. , Rf2 0.8, Rf3 0.67, Rf4 0.6, Rf5 0.43, Rf6 0.25. Furthermore, the results of TLC-Bioautography showed that there were 2 spots that were active in inhibiting all test bacteria with Rf values = 0.8 and 0.25, respectively.
ISOLATION OF SKY ROOT ENDOPHITE (Helminthostachys zeylanica (L.) Hook) PRODUCING ANTIBIOTICS DIFFERENTLY AGAR Mitra, Andi; Herwin, Herwin; Nuryanti, Siska
Journal Microbiology Science Vol 3, No 1 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.907 KB) | DOI: 10.56711/jms.v3i1.952

Abstract

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