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All Journal Jurnal Fitofarmaka Indonesia As-syifaa Jurnal Farmasi PREPOTIF : Jurnal Kesehatan Masyarakat Journal Microbiology Science Jurnal Pengabdian Farmasi dan Sains MPSJ
Siska Nuryanti, Siska
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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SKRINING AKTIVITAS ANTIMIKROBA EKSTRAK HERBA BELIMBING TANAH (OXALIS CORNICULATA L.) SECARA KLT-BIOAUTOGRAFI DAN DIFUSI AGAR Herwin, Herwin; Nuryanti, Siska
Jurnal Ilmiah As-Syifaa Vol 4, No 1 (2012): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (43.169 KB)

Abstract

The screening studies have been carried out antimicrobial activity of herb Belimbing Tanah (Oxalis corniculata L.) extracts from Mamuju West Sulawesi that purpose to got data about activity of Belimbing Tanah (Oxalis corniculata L.) extract based on antimicrobial screening of some microbes in the Bioautography-TLC test and agar diffusion.The result screening of antimicrobial activity by Bioautography-TLC,showed that  dietil eter extract of belimbing tanah (Oxalis corniculata L.) give antimicrobial activity to bacteri Staphylococcus aureus, Streptococcus mutans, Staphylococcus epidermidis, Salmonella thypi, Escherichia coli, Vibrio sp, and fungus Candida albicans  with a profile chromatogram using the eluent n-hexane: ethyl acetate Rf value of 0.45 obtained. And the screening of antimicrobial activity of the juice belimbing tanah (Oxalis corniculata L.) at a concentration of 25%, 50% and 75% in the diffusion in order to obtain an average diameter of zone of resistance to the bacteria Staphylococcus aureus = 14:00 mm, Streptococcus mutans = 15.00 mm, Salmonella thypi = 24.66 mm, Escherichia coli = 19.99 mm, Vibrio sp = 17.77 mm, and fungus Candida albicans = 15.00 mm. Keyword : Oxalis corniculata L., Screening of antimicrobial, Mamuju
AKTIVITAS ANTIFUNGI TEMU PUTIH (Curcuma zedoaria) TERHADAP Trichophyton mentagrophytes Nuryanti, Siska
Jurnal Ilmiah As-Syifaa Vol 8, No 2 (2016): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.484 KB) | DOI: 10.33096/jifa.v8i2.214

Abstract

Curcuma zedoaria has long been known to be used in treatment of dermatophytosis; a skin disease caused by dermatophytes fungi such as Trichophyton mentagrophytes.The fungi infects hairs, skins, and nails that contain keratin. The study was conducted in order to measure the effect of ethanol extract of curcuma zedoaria in inhibiting Trichophyton mentagrohytes colonies. Results showed that the curcuma zedoaria ekstract inhibited Trichophyton mentagrophytes. The research was continued by Minimum Inhibitory Concentration (MIC) test by liquid dilution method in Sabaroud Dekstrosa Broth. Ethanol extract curcuma zedoaria did not indicate the value of the MIC test  because due to the color and viscosity of samples which interfere with the observations. As for the positive control shows a clear color, that  indicating the presence of inhibitory Trichophyton mentagrophytes by ketokonazol. For the value of the Minimum Fungicidal Concentration (MFC) to Trichophyton mentagrophytes are present in a concentration of 50%.
AKTIVITAS ANTIBAKTERI ISOLAT FUNGI ENDOFIT BIJI LABU KUNING (Cucurbita moschata ex. Poir) Nuryanti, Siska; Fitriana, Fitriana; Ohorella, Fitriah Anggraini
As-Syifaa Jurnal Farmasi Vol 15, No 2 (2023): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jifa.v15i2.1069

Abstract

Cucurbita moschata ex. Poir are one of the medicinal plants that have many benefits, including antidiabetic, antifungal, antibacterial, anti-inflammatory, and antioxidant effects. The content of compounds in yellow pumpkin seeds, such as phenols, alkaloids, and triterpenoids, This study aims to isolate endophytic fungi from yellow pumpkin seeds and test the antibacterial activity of isolates of yellow pumpkin bii endophytic fungi using the Agar diffusion method. The results of the isolation of yellow pumpkin seed endophytic fungi obtained 3 isolates, followed by macroscopic examination and fermentation of the 3 isolates with Maltose Yeast Broth (MYB) media and testing antibacterial activity by Agar diffusion method. Based on the results of the study, 3 isolates with the codes IJBL1, IJBL2, and IJBL3 were obtained that have antibacterial potential. The highest inhibitory zone was produced by IJBL3 endophytic fungi isolate with an inhibitory zone diameter of 12.39 mm against Staphylococcus aureus bacteria
IDENTIFICATION AND ANTIBACTERIAL ACTIVITY TEST OF ENDOPHYTE FUNGI MAHONI BARK (Swieenia mahagoni L.) IFKBM04 Nuryanti, Siska; Rosdah, Rosdah; Naid, Tadjuddin; Hardiyanti, Vivi
Journal Microbiology Science Vol 1, No 1 (2021)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.419 KB) | DOI: 10.56711/jms.v1i1.817

Abstract

Endophytes represent a complex community of microorganisms colonizing asymptomatically internal tissues of higher plants. Several reports have shown that endophytes enhance the fitness of their host plants by direct production of bioactive secondary metabolites. This study investigated the antibacterial activity of endophytes fungi IFKBM04 isolated from Swietenia mahagoni L. and identified macroscopic and microscopic characteristics of endophytic fungi. Based on the results of macroscopic and microscopic identification compared with the literature to determine the general characteristics of the sample. The results of this morphological identification showed that the sample of mahogany bark isolate (Swietenia mahagoni L.) code IFKMB-04 had similarities with the genus Saccharomyces sp.
Isolation Of Endophytic Fungi From Aloe Vera Against Bacteria That Cause Digestive Tract Infections By TLC-Bioautography And Agar Diffusion Ramadhana, Evi Rahma; Herwin, Herwin; Nuryanti, Siska
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.993

Abstract

The Aloe vera plant, also known as the Lidah buaya plant, has antibacterial properties due to its secondary metabolites, which include alkaloids, flavonoids, tannins, and saponins. In this study, endophytic fungi from Lidah Buaya (Aloe vera) were isolated against bacteria that cause gastrointestinal tract infections using TLC-Bioautography and Agar Diffusion. In this study, 7 isolates of endophytic fungi were obtained, one of which was IFLB 1 which had potential as an antibacterial, purification of the endophytic fungi was carried out and then macroscopic examination was carried out on the fungi to see the colonies formed. After that, a screening test was carried out for isolates of endophytic fungi. Then the isolates that gave the best activity were reproduced and continued with the fermentation process for 14 days. The extraction results were then evaporated to obtain a thick extract and a Thin Layer Chromatography (TLC) Identification test was carried out in which the eluent used was chloroform: methanol (5:1). Then continued the TLC-Bioautographic Antibacterial Activity Test on Escherichia coli, Salmonella thypi, Shigella dysentriae and Vibrio cholerae bacteria and obtained 2 active spots with Rf1 = 0.78 and Rf2 = 0.30. While the results of testing the antibacterial activity using the Agar Diffusion method obtained the largest diameter of the inhibition zone at a concentration of 4% for Escherichia coli with an inhibition zone diameter of 15.80 mm, Salmonella thypi with an inhibition zone diameter of 14.90 mm, Shigella dysentriae with an inhibition zone diameter of 13 .85 mm and Vibrio cholerae with an inhibition zone diameter of 14.70 mm
Antibacterial Activity of Keji Beling (Strobilanthes crispa) Ethanol Extract Using TLC – Bioautography Junardin, Nurul Fadhillah; Nuryanti, Siska; Asmaliani, Ira
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1037

Abstract

Antibacterials are compounds that inhibit the growth of bacteria that are harm humans. Keji Beling (Strobilanthes crispa) contains secondary metabolites such as saponins, flavonoids, terpenoids, polyphenols, and potassium. One part used in traditional medicine is the leaves which can treat various diseases. In this study, the ethanol extract of Keji Beling leaves was tested using TLC-bioautography This study tested the ethanol extract of Keji Beling leaves using TLC-bioautography. The examination begins with a screening for antibacterial activity against several pathogen bacteria, with results that inhibit the growth of Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Pseudomonas aeruginosa, Streptococcus mutans, Salmonella typhii, Shygella dysenteriae, and Vibrio cholera. Test results using the TLC-bioautography method using n-hexane eluent: ethyl acetate (1: 2) found that the spots were active against bacteria Pseudomonas aeruginosa and Staphylococcus aureus Rf value 0.74 and bacteria Bacillus subtilis, Escherichia coli, Salmonella thypii, Staphylococcus epidermidis, Staphylococcus mutans, Shygella dysenteriae, Vibrio cholera with an Rf value of 0.83 The results of the identification of chemical components showed the presence of positive compounds belonging to the class of flavonoids and anthraquinones. The ethanol extract of keji beling leaves contains a class of flavonoid compounds that have the potential as antibacterial agents with an Rf value of 0.83
Evaluation of Ethanol Extract Tapak Dara Leaf (Catharanthus roseus L. ) for Antibacterial Activity against Skin Pathogens Alhijrah, Yayu; Naid, Tadjuddin; Nuryanti, Siska
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1006

Abstract

Tapak dara leaves (Catharanthus roseus L.) are known to be efficacious for relieving muscle pain, antidepressants, as well as medicine for various diseases such as relieving swelling from wasp stings, nosebleeds, and sore throats, antidotes, antibacterials, and lowering blood pressure in humans because they contain chemical compounds such as essential oils, phenolic acids, flavonoids, tannins, and alkaloids. This study aims to determine the antibacterial activity of the ethanol extract of tapak dara leaves against bacteria that cause skin infections. This type of research is done in an experimental laboratory using the agar diffusion method, which is based on inhibiting the growth of bacteria that cause skin infections. The stages of this research began with sample preparation, screening for antibacterial extract activity, and testing the antibacterial extract activity at several concentrations against the test bacteria that cause skin infections. This study obtained the results that the ethanol extract of tapak dara leaves tested positive for inhibition of the growth of Staphyloccus aureus, Staphylococcus epidermidis, Pseudomonas aeroginosa, and Propionibacterium acne based on the extract screening results. The results of this study also showed that the ethanol extract of tapak dara leaves at a concentration of 40% had the greatest inhibition using the agar diffusion method based on the diameter of the inhibition zone, with a value of 8.37 mm (Propionibacterium acne); 8.47 mm (Pseudomonas aeruginosa); 9.19 mm (Staphylococcus epidermidis); and 11.68 mm (Staphylococcus aureus)
Antibacterial Activity of Tunicata Ethanol Extract Polycarpa Aurata gainst Escherichia Coli and Salmonella Typhi by TLC-Bioautography and Agar Diffusion Selviati, Ratmi; Herwin, Herwin; Nuryanti, Siska
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1040

Abstract

Tunicata Polycarpa aurata contains chemical compounds in the form of peptides and alkaloids  that are cytotoxic and have the ability as antibacterial against several pathogenic bacteria. The aims of this study was to determine the antibacterial activity of the ethanol extract of Tunicata Polycarpa aurata using the TLC-bioautography and diffusion method. Tunicata Polycarpa aurata was extracted by maceration and then evaporated to obtain a thick extract. The results of the antibacterial screening test showed the active extract at a concentration of 0.1% against Escherichia coli and Salmonella typhi. The results of testing for antibacterial activity using the TLC-bioautography with using ethanol: ethyl acetate (1:4) eluent showed results of Salmonella typhi bacteria with an Rf value = 0.27 and Escherichia coli bacteria with an Rf value = 0.27. While the results of testing the antibacterial activity the using the agar diffusion method showed the largest inhibition zona diameter at a concentration of 4% for Escherichia coli bacteria with an inhibition zone diameter of 12.55 mm, and Salmonella typhi with an inhibition zone diameter of 12.3 mm. Phytochemical screening tests on the ethanol extract of Tunicata Polycarpa aurata revealed  the presence of flavonoids, tannins, steroids/terpenoids, alkaloids and saponins.
ISOLATION OF SKY ROOT ENDOPHITE (Helminthostachys zeylanica (L.) Hook) PRODUCING ANTIBIOTICS DIFFERENTLY AGAR Mitra, Andi; Herwin, Herwin; Nuryanti, Siska
Journal Microbiology Science Vol 3, No 1 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.907 KB) | DOI: 10.56711/jms.v3i1.952

Abstract

Antibacterial Activity Test of Ethanol Extract of Basil Leaves (Ocimum basilicum) Against Streptococcus mutans and Porphyromonas gingivalis Soamole, Nurul Fatimah; Nuryanti, Siska
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1024

Abstract

Basil leaves (Ocimum basilicum) is a type of plant used traditionally that has antibacterial potential. Antibacterial compounds are compounds that can inhibit the growth of bacteria. This study aims to obtain ethanol extract of basil leaves (Ocimum basilicum) to determine the activity and diameter of the largest inhibition zone against Streptococcus mutans and Porphyromonas gingivalis bacteria. This research was started by preparing a thick extract of basil leaves which was obtained by extracting basil leaves by maceration. Antibacterial screening test with concentrations of 0.1% and 0.5% against Streptococcus mutans and Porphyromonas gingivalis. The method used was agar diffusion with 6 concentrations in each treatment group of ethanol extract of basil leaves with concentrations of 0.5%, 1%, 5%, 10%, 15% and 30%. The results of the screening test gave activity at a concentration of 0.5% against the two test bacteria. The results of the antibacterial activity test of the ethanol extract of basil leaves against the test bacteria obtained the largest diameter of the inhibition zone at a concentration of 30%. Based on research, the ethanol extract of basil leaves has activity as an antibacterial.
Co-Authors Akbar, Muhammad Alief Akihito mr, Andi Al Kahfi Alhijrah, Yayu Ancha, Annisa Anggraeni, Nur Afni Asriani Suhaenah Bayu Putra, Bayu Evatiara, Nur faridah, susanti Fitriana Fitriana Fitriana Hardiyanti, Vivi Herwin Herwin Herwin, Herwin hikma, Nurhikma Irfanitha, irfanitha Junardin, Nurul Fadhillah Kayla, Aulia Putri Mitra, Andi Musfatir, Harvanita Nurung, Ayyub Harly Ohorella, Fitriah Anggraini Priatna, Nabilah Hana Putra, Aryadi Surya Putria, Yashifa Anandita R. Rusli Rabbana, Rahmawati Rachman, Moch. Erwin Rachmat Faisal Syamsu, Rachmat Faisal Rachmat Kosman, Rachmat Rahman, Safriana Rahmayanti, Hani Rahmayanti Ramadhan, Wahyu Gilang Ramadhana, Evi Rahma Rosdah, Rosdah Rusli Saputra, Muh. Satrio Surya Saputri, Febrianti Ayu Selviati, Ratmi Seminarwati, Sri Sitti Amirah Soamole, Nurul Fatimah Tadjuddin Naid Umaiyah, Ummi Putri Zainal Abidin