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Ayyub Harly Nurung
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Editor's Address: 3rd Floor Laboratory Faculty of Pharmacy, 2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia
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INDONESIA
Journal Microbiology Science
Published by Universitas Muslim Indonesia
ISSN : 2808392X     EISSN : 28083911     DOI : https://doi.org/10.56711/jms
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Journal Microbiology Sciences (J. Mic. Sciences) receives writing in the form of research results, scientific studies and brief descriptions relating to Microbiology, such as Molecular and Microbial Epidemiology Clinical Microbiology Medical Microbiology Pharmaceutical Biotechnology and others related to Microbiology Sciencee
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 9 Documents
 1 
Search results for , issue "Vol 5, No 1 (2025)" : 9 Documents clear
Antibacterial Activity Test of Ceremai Leaf Ethanol Extract (Phyllanthus acidus (L.) Skeels) Against Bacteria Causing Skin Infections by KLT-Bioautography and Agar Diffusion Naid, Tadjuddin; Herwin, Herwin; Muldin, Nurul Insani
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1204

Abstract

Ceremai leaves (Phyllanthus acidus) are known for their medicinal properties, including the treatment of infectious diseases. This study aims to evaluate the antibacterial activity of ethanol extract from Ceremai leaves against skin infection-causing bacteria using Thin Layer Chromatography-Bioautography (TLC-Bioautography) and agar diffusion methods. The study began with an antibacterial screening test, followed by TLC-Bioautography, and then determination of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) at various concentrations (0.125% to 16%). The agar diffusion method was used to further assess antibacterial activity. Screening results showed antibacterial activity at a concentration of 1%. TLC-Bioautography with a chloroform: methanol: water (20:2:0.5) eluent revealed active spots with Rf values of 0.8, 0.54, 0.36, and 0.29. The MIC values were 0.25% for P. acnes and S. aureus, and 0.5% for P. aeruginosa and S. epidermidis. The MBC values were 0.5% for P. acnes and S. aureus, and 1% for P. aeruginosa and S. epidermidis. The largest inhibition zone in the agar diffusion test was observed at a 32% concentration against S. aureus, measuring 20.11 mm. These findings suggest that Ceremai leaf extract has significant antibacterial potential and could be considered for developing alternative treatments for skin infections.
Antibacterial Activity of Ethanol Extract of Krokot Herb (Portulaca oleracea L.) against Skin Infection Pathogens: Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes Annisa, Andi Adria
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1163

Abstract

Purslane (Portulaca oleracea L.), a traditional herb, is rich in various secondary metabolites like flavonoids, phenolics, tannins, and saponins, known for their antibacterial properties. This study evaluates the antibacterial efficacy of ethanol extract of purslane against skin-infecting bacteria. Using the Agar diffusion method, the study tested the extract's activity against Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes. The results indicate significant antibacterial activity, with the highest inhibition observed at a 32% concentration, producing inhibition zones of 10.29 mm, 9.05 mm, and 8.59 mm against S. aureus, S. epidermidis, and P. acnes, respectively. These findings suggest that purslane extract could be an effective antibacterial agent for treating skin infections. This study contributes to the understanding of the potential applications of purslane in medical treatments, emphasizing the need for further investigation into its bioactive compounds.
Volume 5(1) 2025 Science, Journal Microbiology
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1339

Abstract

Antibacterial Activity of Etanol Extract of Pinang (Areca catechu L.) Clup against Streptococcus mutans and Porphyromonas gingivalis Nuryanti, Siska; Herwin, Herwin; Rahmayanti, Hani Rahmayanti
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1178

Abstract

Areca nut (Areca catechu L.) is a plant that has many uses and can be used for traditional medicine. Areca nut skin can be used to treat indigestion (dyspepsia), edema and beriberi due to low urine output. This study aims to determine the antibacterial activity of ethanol extract of areca nut peel against the test bacteria Streptococcus mutans and Porphyromonas gingivalis. In this research, the areca nut peel simplicia was extracted using the maceration method with 96% ethanol solvent, after which a liquid ethanol extract was obtained and then evaporated using a rotary vacuum evaporator to obtain a thick extract. After the extract is thick, a Minimum Inhibitor Concentration (MIC) test and a Minimum Bactericide Concentration (MBC) test are carried out. The results obtained in the Minimum Inhibitor Concentration (MIC) test and Minimum Bactericide Concentration (MBC) test on Streptococcus mutans and Porphyromonas gingivalis bacteria were 0.5%. Antibacterial activity test using the agar diffusion method was obtained, for Streptococcus mutans bacteria, the largest inhibitory zone diameter at a concentration of 16% was 12.58 mm and for Porphyromonas gingivalis bacteria at a concentration of 16%, namely 10.93 mm. Based on research, ethanol extract of areca nut peel has antibacterial potential.
Antimicrobial Activity of Ethanol Extract of Pinang Seed (Areca catechu L.) Against the Growth of Candida albicans and Malassezia furfur Fungi Using the Agar Diffusion Method Naid, Tadjuddin; Saputri, Febrianti Ayu; Nuryanti, Siska
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1229

Abstract

Pinang seeds (Areca catechu L.) are known for their various benefits, including antimicrobial properties. This study aims to evaluate the antimicrobial activity of ethanol extract from pinang seed against the growth of Candida albicans and Malassezia furfur fungi using the agar diffusion method. The research involved extracting the pinang seed using maceration with 96% ethanol, followed by evaporation using a rotary vacuum evaporator to obtain a concentrated extract. Antimicrobial activity was tested across concentrations of 32%, 16%, 8%, 4%, 2%, 1%, 0.5%, and 0.1%. The results showed that at a concentration of 32%, the ethanol extract produced the largest inhibition zones: 27.78 mm against Candida albicans and 15.51 mm against Malassezia furfur, both categorized as very strong activity. These findings suggest that ethanol extract of pinang seed has significant potential as an antimicrobial agent, particularly against fungal pathogens.
Antibacterial Activity of Ethanol Extract of Cocoa Leaves (Theobroma cacao L.) Against Gastrointestinal Infection Bacteria Tract Infection by Agar Diffusion Method Sya’baniyah Herman, Nur Fajriah; Fitriana, Fitriana; Maryam, St.
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1218

Abstract

The cocoa plant (Theobroma cacao L.) leaves contain secondary metabolites, including flavonoids, saponins, and tannins, known for their potential antibacterial properties. This study aims to evaluate the antibacterial activity of the ethanol extract of cocoa leaves (Theobroma cacao L.) against bacteria that cause gastrointestinal infections using the agar diffusion method. The study assessed the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extract. The results showed that the ethanol extract exhibited significant antibacterial activity, particularly at concentrations of 2.5%, 5%, and 10%, with the highest inhibition zone observed at 10% concentration. The extract was most effective against Escherichia coli, Salmonella typhi, Shigella dysenteriae, and Vibrio cholerae, with inhibition zones of 15.34 mm, 15.60 mm, 13.81 mm, and 16.43 mm, respectively. These findings suggest that cocoa leaf extract has the potential to be developed as an antibacterial agent against gastrointestinal pathogens.
Characterization of Cellulose-Producing Bacterial Isolates from the Areca Nut (Areca catechu L.) Nuryanti, Siska; Herwin, Herwin; hikma, Nurhikma
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1179

Abstract

Areca nut peel (Arecha catechu L.), a byproduct of areca nut processing, holds potential as a raw material for bioplastics through cellulose utilization. This study aimed to identify bacterial species in areca nut peel capable of producing cellulose. Employing bacterial isolation and identification methods, including morphological and biochemical tests, four bacterial isolates were obtained. Two isolates, identified as gram-negative, were classified under the genus Acetobacter, and two gram-positive isolates were identified as belonging to the genus Lactobacillus. These findings demonstrate the potential use of cellulose from areca nut peel in bioplastic production.
Antibacterial Activity of the Ethyl Acetate Fraction of Qust Al-Hindi (Saussurea lappa) in Inhibiting Escherichia coli Growth Using the Agar Diffusion Method Mulya, Esti Lestari; Sukmawati, Sukmawati; Fitriana, Fitriana
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1215

Abstract

The root of the Qust al Hindi plant (Saussurea lappa) has been recognized for its potential in treating infectious diseases. This research aims to evaluate the antibacterial activity of the ethyl acetate fraction of Qust al Hindi roots against digestive tract bacterial infections, specifically targeting Escherichia coli, using the agar diffusion method. The study commenced with an initial screening test at a concentration of 1%, followed by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests at concentrations of 0.25%, 0.5%, 1%, 2%, 4%, 8%, and 16%. The MIC was determined to be 1%, while the MBC was observed at 8%. Further antibacterial activity testing was conducted at concentrations of 32%, 16%, and 8%, with the optimal activity noted at 32%
Antioxidant Activity Analysis of Endophytic Fungal Isolates IFBZ5, IFBZ6, and IFBZ7 from the Stem of Bidara (Ziziphus mauritiana Lam.) Fitriana, Fitriana; Tahir, Masdiana; Hamdhan, Aulia Nurul Insani; Sumardi, Farah Hikmah Nur; Putri, Adinda
Journal Microbiology Science Vol 5, No 1 (2025)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i1.1193

Abstract

Endophytic fungal isolates coded IFBZ5, IFBZ6, and IFBZ7 derived from the stem of the bidara plant (Ziziphus mauritiana Lam.) have shown potential antioxidant properties. This study aims to test the antioxidant activity of endophytic fungal isolates on the bidara plant stem (Ziziphus mauritiana Lam.) by TLC-Autography method and DPPH (1,1-diphenyl-2-picrylhydrazil) free radical silencing. This study used endophytic fungi isolates code IFBZ-5, IFBZ-6 and IFBZ-7 which were fermented for 21 days using MYB medium which was then liquid-liquid extracted with ethyl acetate (1:1) and evaporated. The fermentate extract obtained was then tested for antioxidant activity qualitatively and quantitatively. Qualitative analysis using TLC plates indicated antioxidant activity in all isolates. Quantitative analysis showed that isolate IFBZ7 exhibited strong antioxidant activity with an IC50 value of 50.120 ppm, while IFBZ5 and IFBZ6 showed very weak activity with IC50 values of 2.482.363 µg/mL and 467.847 µg/mL, respectively. The BHT comparator had an IC50 value of 6.778 µg/mL. This shows that the antioxidant power of endophytic fungal isolates coded IFBZ5, IFBZ6 and IFBZ7 is lower than the antioxidant activity of BHT as a comparison.

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