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Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Published by Universitas Syiah Kuala
ISSN : 20897790     EISSN : 25026194     DOI : 10.13170/depik
Core Subject : Science, Education, Engineering,
Earth & Planetary Sciences Education Engineering
Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan is a peer review international journal, this journal is publishing high-quality articles in aquatic sciences and fisheries in general. The aim of the journal is to publish and disseminate the current or new findings of the research, and give a significant contribution to the development of fisheries and aquatic sciences in several topics, but not limited to: Fisheries (Aquaculture, Capture Fisheries, Fish Processing) Aquatic Ecology (Freshwater, Marine, and Brackishwater) Aquatic Biology (Fish, Mollusk, Crustacean, Plankton, Coral reefs) Oceanography.
Arjuna Subject : Ilmu Bumi dan Planet (semua kategori) - Ilmu Bumi dan Planet (Lain-Lain)
Articles 13 Documents
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Search results for , issue "Vol 6, No 3 (2017): December 2017" : 13 Documents clear
Kajian kualitas perairan Teluk Gorontalo dengan menggunakan metode STORET Miftahul Khair Kadim; Nuralim Pasisingi; Afriani R. Paramata
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.6.3.8442

Abstract

Good water quality is extremely important to support life of organisms. The determination of water quality status was needed as reference to monitor water pollution in aquatic system. This study aimed to assess the status of water quality in the Gorontalo Bay. There ware five sampling sites which represented the coastal condition and ecological characteristic. Sampling was carried out in May-July 2017. Sampling procedure of water samples carried out based on APHA standard method. Existing water quality data was analyzed by using STORET method. The result showed that Gorontalo bay obtained total score -39. It means the status of water quality the Gorontalo bay during sampling period in bad category. Kondisi kualitas air suatu perairan yang baik sangat penting untuk mendukung kelulushidupan organisme yang hidup di dalamnya. Penentuan status mutu air perlu dilakukan sebagai acuan dalam melakukan pemantauan pencemaran kualitas air suatu sistem akuatik. Penelitian ini bertujuan untuk mengkaji status mutu air di perairan Teluk Gorontalo. Terdapat 5 stasiun pengamatan yang dipilih berdasarkan kondisi pantai dan karakteristik ekologi Teluk Gorontalo. Pengambilan sampel parameter kualitas air dilakukan di sepanjang perairan Teluk Gorontalo pada bulan Mei-Juli 2017. Prosedur pengambilan sampel berdasarkan metode baku APHA. Data kualitas air yang ada kemudian akan dianalisis dengan menggunakan Metode STORET. Hasil penelitian menunjukkan bahwa Teluk Gorontalo memperoleh total skor -39. Hal ini menunjukkan status mutu Teluk Gorontalo di bulan Mei-Juli tergolong dalam kategori buruk. 
Status klorofil-a di Perairan Teluk Pria Laot Sabang, Provinsi Aceh Sri Agustina; Musri Musman; Muhammad Ishaq
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.6.3.8364

Abstract

Study about chlorophyll-a state in Teluk Pria Laot Sabang has been done in October 2016. The objective of this study was to analyze the chlorophyll-a concentration and phytoplankton abundance in Teluk Pria Laot Sabang. Samples were collected at a station located at 5,84653oN and 95,29001oE during 4 days (4, 6, 8, and 10th October 2016) which each day had 4 sampling times (07:00 am, 10:00 am, 01:00 pm, and 04:00 pm). Chlorophyll-a, phytoplankton, and nutrient analysis were conducted at chemistry laboratory of Teacher Training and Education Faculty, Syiah Kuala University, marine chemistry laboratory of Marine and Fisheries Faculty, Syiah Kuala University, and Unit of Environment Research and Development, Banda Aceh. The result showed that chlorophyll-a concentration ranged 0,02  – 1,7 µg/L withthe highest average (0,64 µg/L) was obtained at 01:00 pm. In addition,phytoplankton abundance was found ranged3539,4 ind/L – 8687,3 ind/L andthe highest average (6435,0 ind/L) appeared at 10:00 am. Nitrate and phosphate concentration which were above the minimum value of threshold revealed that this area was rich in nutrient availabilityto support the phytoplankton growth. Chlorophyll-a concentrations are likely influenced by light intensity with rich nutrient availability than phytoplankton abundance. According to chlorophyll-a concentration, Teluk Pria Laot Sabang is categorized as Oligothropic area.Penelitian tentang status klorofil-a di Teluk Pria Laot Sabang telah dilakukan pada Bulan Oktober 2016. Tujuan dari penelitian ini adalah untuk mengetahui konsentrasi klorofil-a dan kelimpahan fitoplankton di Teluk Pria Laot Sabang. Sampel dikumpulkan pada stasiun yang terletak pada koordinat 5,84653oLU dan 95,29001oBT selama 4 hari dengan 4 kali waktu pengambilan per hari (pukul 07.00, 10.00, 13.00, dan 16.00 WIB). Analisis klorofil-a, fitoplankton dan nutrien dilakukan di Laboratorium FKIP – kimia Unsyiah, Laboratorium Kimia Laut, FKP Unsyiah, Balai Penelitian dan Pengembangan Lingkungan (BPPL) Banda Aceh. Hasil penelitian menunjukkan bahwa konsentrasi klorofil-a berkisar 0,02– 1,7µg/Ldengan nilai rata – rata konsentrasi klorofil-a tertinggi 0,64µg/L didapatkan pada waktu pengambilan pukul 13:00 WIB. Kelimpahan fitoplankton yang diperoleh berkisar 3539,4 ind/L – 8687,3 ind/L dengan rata – rata tertinggi dengan nilai 6435,0 ind/L diperoleh pada pukul 10:00 WIB. Konsentrasi nitrat dan fosfat yang berada di atas ambang batas minimal menggambarkan bahwa daerah ini menyuplai nutrien yang cukup untuk pertumbuhan fitoplankton. Fluktuasi konsentrasi klorofil-a lebih cenderung mengikuti perubahan intensitas cahaya matahari dengan ketersediaan nutrien (nitrat dan fosfat) yang cukup. Berdasarkan konsentrasi klorofil-a yang diperoleh, Perairan Teluk Pria Laot Sabang dikategorikan sebagaidaerah oligotrofik.
Karakteristik pH dan pengaruhnya terhadap bakteri Coliform di perairan Selat Madura Kabupaten Pamekasan Eva Ari Wahyuni
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.6.3.5875

Abstract

The objective of present study was to analyze the condition of environmental parameters, especially pH and its relationship to coliform survival rate which is useful to understand the status of the aquatic environment in Pamekasan District. There are three sampling locations with three depth profiles and determine using purposive sampling method. Environmental parameters are measured on surface profiles, including DO, temperature, pH, and salinity. Seawater samples were cultured on agar medium and selective media, then analyzed using colony counting method to calculate the number of bacteria in each depth profile. The results showed the DO range of 5.4 to 5.6 mg/L, the temperature 31.1-32.20C, pH 7.4-8.0, and the salinity 31-32‰, and the number of coliform bacteria ranged 15-85 (Colony x 10 CFU/mL) found on the surface and bottom of stations 2 and 3. The pH fluctuations during the study were higher than 2012 and 2013. The pattern of coliform bacteria distribution showed lower with increasing depth. Differences in the pH range are thought to be one of the factors that affect the survival rate of coliform bacteria, so that also affect the density of coliform bacteria. In general, it can be concluded that environmental conditions in good status based on environmental parameter indicators are relatively normal for tropical regions. The pH range is quite wide with a tendency to increase in value (indicating more alkaline) during the study period. Changes in pH have an effect on the survival rate with a higher tendency to decrease in acidic conditionsPenelitian ini bertujuan untuk menganalisis kondisi parameter lingkungan, khususnya pH dan keterkaitan terhadap perkembangbiakan (survival rate) bakteri coliform yang berguna untuk mengetahui status lingkungan perairan di Kabupaten Pamekasan. Lokasi pengambilan sampel air laut dilakukan pada tiga stasiun dengan tiga profil kedalaman menggunakan metode purposive sampling. Parameter lingkungan diukur pada profil permukaan, meliputi DO, suhu, pH, dan salinitas. Sampel air laut dibiakkan pada media agar dan media selektif, kemudian dianalisa menggunakan metode colony counting untuk menghitung jumlah bakteri pada setiap profil kedalaman. Hasil analisa menunjukkan kisaran DO 5,4-5,6 mg/L, suhu 31,1-32,20C, pH 7,4–8,0, dan salinitas 31-32 ‰, dan jumlah bakteri coliform berkisar 15-85 (koloni x 10 cfu/mL) yang ditemukan pada profil permukaan dan dasar pada stasiun 2 dan 3. Fluktuasi pH selama penelitian lebih tinggi dibandingkan penelitian tahun 2012 dan 2013. Pola sebaran bakteri coliform menunjukkan semakin rendah dengan bertambahnya kedalaman. Perbedaan kisaran pH diduga menjadi salah satu faktor yang berpengaruh terhadap survival rate bakteri coliform, sehingga berpengaruh juga terhadap kepadatan bakteri coliform. Secara umum dapat disimpulkan bahwa kondisi lingkungan dalam status baik berdasarkan indikator parameter lingkungan relatif normal untuk wilayah tropis. Kisaran pH cukup lebar dengan kecenderungan semakin bertambah besar nilainya (menunjukkan semakin basa) selama periode penelitian. Perubahan pH berpengaruh terhadap survival rate dengan kecenderungan lebih tinggi penurunannya pada kondisi asam
Kandungan merkuri pada substrat dasar di kawasan muara Krueng Sabee, Krueng Panga, dan Krueng Teunom, Kabupaten Aceh Jaya Syahrul Purnawan; Rifki Rahman; Sofyatuddin Karina
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.6.3.8108

Abstract

The objective of this study was to determine the mercury content in sediment in the waters of Aceh Jaya coastal. The research was carried out in the Estuary of Krueng Sabee, Panga, and Teunom River on October 2015. Samples were analyzed using the Atomic Absorption Spectrophotometer (AAS) at the Laboratory of Chemistry, Mathematics and Natural Sciences Faculty, Syiah Kuala University. It was found that the Hg content in sediment of Krueng Sabee, Panga, and Teunom were 0.76, 0.68, and 1.03 mg/kg, respectively. Hg metal content in the sediment at all stations remained below the threshold. Despite,  the correlation between Hg content and sediment size in this study was weak, but there was a tendency the finer sediment size the higher Hg content found in sediment. However, heavy metal from water was easier to be deposed and embedded in fine sediment.Tujuan dilakukannya penelitian ini adalah untuk mengetahui sebaran logam berat merkuri (Hg) yang terkandung pada substrat dasar di beberapa muara sungai Kabupaten Aceh Jaya. Pengambilan data dilakukan pada Bulan Oktober 2015 di Krueng Sabee, Krueng Panga, dan Krueng Teunom. Sampel substrat diambil berdasarkan metode coring menggunakan pipa paralon, Atomic Absorption Spectrophotometer (AAS) atau Spektrofotometer Serapan Atom digunakan untuk mendapatkan data kandungan logam berat Hg pada substrat, sementara metode ayak basah digunakan untuk mendapatkan data ukuran butiran. Kandungan merkuri (Hg) dalam substrat Krueng Sabee, Krueng Panga, dan Krueng Teunom masing-masing adalah 0,76; 0,68 dan 1,03 mg/kg. Terlihat kecenderungan substrat dengan butiran yang halus mengandung kadar merkuri yang lebih tinggi, dimana sifat merkuri yang mudah berikatan dengan zat organik mempermudah pengendapan pada bentuk butiran yang halus.
Analisis kesesuaian dan daya dukung lingkungan untuk pengembangan wisata bahari di Pulau Bawean Kabupaten Gresik Provinsi Jaya Timur Sukandar Sukandar; Citra S. U. Dewi; Muliawati Handayani
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.6.3.7024

Abstract

Bawean Island has potency as a ecotourism destination in Gresik District, Province of Jaya timur. This island is one of the popular destination for local tourist, and the visitors are increasing over the years; but there was no study on the suitability and carrying capacity of the island for marine ecotourism activities. The purpose of this study was to analysis the suitability and carrying capacity of Bawean Island for marine tourism. The Results of the analysis showed that the area of marine ocotourism in Bawean is Suitable (S1) for marine ecotourism development with a value for leisure travel 88.33%, snorkeling activity was 84.21%; and diving 81.48%. The total value carrying capacity of region was 398 persons/day.Pulau Bawean memiliki potensi sebagai daerah tujuan wisata bahari di kabupaten Gresikproins Jaya timur. Pulau ini telah menjadi tujuan wisata lokal bagi masyarakat Jaya timur dan jumlah kunjungan wisatawan semakin meningkat dari tahun ke tahun. Namu belum ada kajian mengenai kesesuaian dan daya dukung lingkungan di Pulau Bawean sebagai kawsan wisata bahari. Oleh karena itu penelitian ini bertujuan untuk mengkaji kesesuaian dan daya dukung Pulau Bawean sebagai kawasan wisata bahari. Hasil analisis menunjukkan bahwa Pulau Bawean sesuai (S1) untuk dikembangkan sebagai kawasan wisata bahari  dengan nilai untuk wisata rekreasi pantai sebesar 88,33%, wisata snorkeling sebesar 84,21% dan wisata selam sebesar 81,48%. Nilai total daya dukung kawasan sebesar 398 orang/hari.
Hubungan panjang berat dan faktor kondisi ikan belodok (Famili: Gobiidae) pada ekosistem mangrove di Desa Pulau Sembilan Kabupaten Langkat Provinsi Sumatera Utara Bill Maulana Bidawi; Desrita Desrita; Yunasfi Yunasfi
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.6.3.7029

Abstract

The aims of the research was to examine the lenght weight relationships, growth pattern and condition factor of mudskipper (Family: Gobiidae) at the mangrove ecosystem. The research was conducted for two months from March to April 2016 at the mangrove ecosystem in the Sembilan Island village. The result showed that there are three species of mudskipper found in study area, namely Periophthalmus chrysospilos, Periophthalmus gracilis, and Boleophthalmus boddarti.  The growth pattern of mudskipper with a value b 3,  indicate a allometrik negative growth pattern and the condition factor was between 1-2.Penelitian ini bertujuan untuk menganalisis hubungan panjang bobot, pola pertumbuhan dan faktor kondisi ikan belodok (Famili: Gobiidae) pada ekosistem mangrove di di desa Pulau Sembilan Langkat. Penelitian dilaksanakan selama dua bulan yaitu Maret sampai April 2016. Penelitian ini menggunakan metode purposive random sampling. Hasil penelitian ditemukan 3 spesies ikan belodok di lokasi penelitian yaitu Periophthalmus chrysospilos, Periophthalmu sgracilis, dan Boleophthalmus boddarti. Pola pertumbuhan ikan belodok dengan nilai b 3, mengindikasikan pola pertumbuhan allometrik negatif. Faktor kondisi dari Ikan Belodok berkisar 1 – 2.
Kajian awal kadar merkuri (Hg) dalam ikan dan kerang di Teluk Kao, Pulau Halmahera Edward Edward
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.6.3.7748

Abstract

The aim of the study was to determine the concentration of Hg  in some species of fish and mussels harvested from Kao Bay . Fish and mussels samples were purchased from fishermen at Kao Bay  in November 2015. The Hg concentration was measured by using Atomic Absorption Spectrophotometer (AAS).The results showed that the highest concentration of Hg was found in gurara fish (Nemipterus japonicus)  that is 0.98 ppm, followed by suo fish  (Sphyraena jello)  0.89 ppm,  tatameri fish (Gazza minuta)  0.38 ppm, gaca fish (Lutjanus argentimaculatus) 0.31 ppm, totodi fish (Synodus foetens)  0.24 ppm,  bubara fish (Caranx sp) 0, 19 ppm, ngafi fish (Stolephorus indicus) 0.19 ppm and biji nangka fish (Upeneus vittatus) 0.15 ppm. In the shelfish meat, the highest concentration of Hg is found in the blood mussels (Anadara granosa), that is 0.42 ppm, and then followed by papaco (Telescopium telescopium) 0.05 ppm. The concentration of Hg in all samples of fish and shelfish were below from the threshold value for seafood fish and shellfish of 0.5 ppm and 1.0 ppm, respectively.Penelitian ini bertujuan untuk mengetahui kadar Hg dalam beberapa jenis ikan dan kerang yang ada di Teluk Kao dalam kaitannya dengan kesehatan makanan hasil laut. Contoh ikan dan kerang di beli dari nelayan di Teluk Kao pada bulan November 2015. Kadar Hg  diukur dengan menggunakan Spektrofotometer Serapan Atom (AAS). Hasilnya menunjukkan kadar Hg tertinggi dalam ikan dijumpai dalam daging ikan gurara (Nemipterus japonicus) yakni 0,98 ppm, selanjutnya diikuti oleh ikan suo (Sphyraena jello) 0,89 ppm, ikan tatameri (Gazza minuta) 0,38 ppm, ikan gaca (Lutjanus argentimaculatus) 0,31 ppm, ikan totodi (Synodus foetens) 0,24 ppm, ikan bubara (Caranx sp) 0,19 ppm, ikan ngafi (Stolephorus indicus)  0,19 ppm, dan ikan biji nangka (Upeneus vittatus) 0,15 ppm. Dalam daging kerang kadar Hg tertinggi dijumpai dalam kerang darah (Anadara granosa) yakni 0,42 ppm selanjutnya dikuti oleh kerang papaco (Telescopium telescopium) 0,05 ppm. Kadar Hg dalam semua contoh ikan dan kerang masih di bawah nilai ambang batas kadar yang diperkenankan dalam  makanan hasil laut yakni 0,5 ppm untuk ikan dan 1 ppm untuk kekerangan. 
Pangan fungsional dan nutrasetikal dari laut: Prospek dan tantangannya Evi Amelia Siahaan; Ratih Pangestuti
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.6.3.6874

Abstract

The marine environment is a rich sources of natural bioactive compounds which are not used optimally. It has been established that marine organisms contain a unique physicochemical characteristic that is very valuable to be applied for food industry and pharmaceutical. Recently, numerous study have been shown that compounds extracted from marine organisms possess various biological activities, including anticoagulant, anticancer and hypocholesterolemic. Moreover, fish oil and marine bacteria have been claimed as a source of omega-3 while crustaceans and seaweeds contained carotenoids and phenolic compounds. Based on a variety of biological activities of marine organisms, this review focuses on the potential use of marine-derived compounds as functional food ingredients and nutraceutical including some consideration of barriers for their application.Laut menyimpan sumber bahan fungsional yang relatif belum dimanfaatkan secara optimal. Karakteristik fisikokimianya yang kompleks dan tidak ditemukan pada biota terrestrial,   menjadikan bahan fungsional laut potensial untuk diaplikasikan dalam berbagai aspek seperti pengolahan makanan, pengawetan, fortifikasi dan industri farmasi. Selain itu, banyak penelitian menunjukkan bahwa senyawa yang diekstrak dari biota laut memiliki aktivitas biologis yang beragam. Sebagai contoh, peptida yang diisolasi dari hidrolisat protein ikan serta fucans, galaktan dan alginat dari alga telah terbukti memiliki aktifitas antikoagulan, antikanker dan hipokolesterolemik. Selain itu, minyak ikan dan bakteri laut merupakan sumber yang asam lemak omega-3, sedangkan krustasea dan rumput laut mengandung senyawa antioksidan seperti karotenoid dan senyawa fenolik. Berdasarkan beragam aktifitas bioaktif yang terkandung pada bahan laut, review ini berfokus pada potensi senyawa laut sebagai bahan pangan fungsional dan nutrasetikal sekaligus tantangan dalam pemanfaatannya.
Efektifitas Nannochloropsis sp. terhadap sistem imun non-spesifik ikan mas Cyprinus carpio yang diinfeksi Virus Herpes Arafik Lamadi
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.6.3.8420

Abstract

The aim of this research was to determine the effect of Nannochlorpsisis sp. against the common carp (Cyprinus carpio) leukocyte profile, infected with Koi Hepesvirus (KHV). The  descriptive observational method wa used in this study. This research was conducted for 4 weeks, using common carp length 12±0,3 cm as many as 7 fish. The tested experiment was P1: without infested by Nannochlorpsisis sp. and KH; P2: Infested by  Nannochlorpsisis sp. and without KHV infected; P3: Infected by Nannochloropsis sp. and KHV; P4;  Not infected by Nannochlorpsisis sp. and  infected by KHV. Evey tratment in three replications. The results showed that the highest total leukocyte was obtained in P3 (37.600 cells/ml), followed by  P4 (29.650 cells / ml), P2 (28,500 cells / ml) and P1 (21,150 cells / ml). Leukocyte differentiation test on P1 (neutrophil 6.2%, lymphocyte 68.0%, monocyte 17.0%), P2 (neutrophil 6.6%, lymphocyte 72.3%, monocyte 18.3%), P3 (neutrophil 7.9%, lymphocyte 66.1%, monocytes 24.3%), P4 (neutrophils 11.2%, lymphocytes 57.6%, monocytes 37.6%). The higher survivla rate was recorded in P2 (100%). It is concluded that  Nannochloropsis sp. can enhance the common carp immune sytem.Penelitian ini bertujuan untuk mengetahui pengaruh pemberian Nannochlorpsis sp. terhadap profil leukosit ikan mas (Cyprinus carpio) yang terinfeksi Koi Herves Virus (KHV). Penelitian ini menggunakan metode deskriptif observasional dengan membandingkan hasil pada perlakuan 1) P1: (tanpa pemberian Nannochlorpsis sp. dan tidak diinfeksi KHV), 2) P2 (diberikan Nannochlorpsis sp dan tanpa diinfeksi KHV), 3) P3 (diberikan Nannochlorpsisis sp dan diinfeksi KHV), 4) P4 (tanpa pemberian Nannochlorpsis sp. dan diinfeksi KHV), masing-masing perlakukan 3 kali ulangan. Penelitian ini dilakukan selama 4 minggu dengan menggunakan Ikan mas berukuran 12±0,3  cm sebanyak 120 ekor. Hasil perhitungan total leukosit tertinggi diperoleh pada P3 (37.600 sel/ml), selanjutnya berturut-turut  P4 (29.650  sel/ml), P2 (28.500 sel/ml) dan P1 (21.150 sel/ml). Hasil uji diferesnsiasi leukosit pada P1 (neutrofil 6.2%, limfosit 68.0%, monosit 17.0%), P2 (neutrofil 6.6%, limfosit 72.3%, monosit 18.3%), P3 (neutrofil 7.9%, limfosit 66.1%, monosit 24.3%), P4 (neutrofil 11.2%, limfosit 57.6%, monosit 37.6%). Kelangsungan hiudp tertinggi diperoleh pada perlakuan P2 (100%). Dapat disimpulkan bahwa Nannochloropsis sp. dapat digunakan untuk meningkatkan kekebalan tubuh ikan mas.
Resistensi dan karakter molekuler benih gurami sowang Osphronemus goramy Lacepede, 1801 asal induk berbeda Kusbiyanto Kusbiyanto; Agus Nuryanto; Petrus H. T. Soedibja
Depik Vol 6, No 3 (2017): December 2017
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.6.3.6539

Abstract

The cultivation of giant gourami is constrained by its high mortality due to low resistance to diseases. Resistance is an inherited character from the parental to their seeds. High resistance seeds can be selected using molecular marker, such as Major Histocompatibility complex (MHC) gene. Resistance character is assumed to be different among individual from different broodstocks and is suggested related to their genetic constituent.  This research aims to analyze the resistance of sowang gouramy seeds from different broodstocks and describe genetic character of seeds from different broodstocks. An explorative survey was perfomed. One hundred individuals were taken purposively from Balai Benih Ikan Sikamaju Ciamis West Java and hundred individuals were bought from fish farmer in Ciamis West Java. Sampling was performed in 2015 for the first seed group and in 2016 for the second seed group. Samples were subjected to Aeromonas hydrophila and Pseudomonas aeruginosa infection. The fragments of MHC gene were amplified using PCR technique from eight individual of first group and six individuals of second group. The resistance characteristic was analyzed using simple mathematics based on the number of living seeds compared total infected seeds and molecular characteristics was analyzed descriptively based on DNA band pattern. Different resistance to A. hydrophila was observed between seed groups from different broodstocks. The seeds group from the first broodstocks showed lower resistance level with the value of 29% than that from the second broodstocks with the resistance value reached of 100%. Both seed groups also showed resistance differences to P.aeruginosa, although  not as high as A.hydrophila infection. The differencies were also reflected in their MHC gene between seed groups from two different broodstocks. The amplication of MHC gene of the seeds from the first broodstacks resulted of 585 bp and 400 bp length fragments, while from those second broodstocks was only resulted of 400 bp frament. The differences on DNA band pattern between seed groups indicate a different molecular characteristics among seeds from different broodstocks.Usaha budidaya ikan gurami terhambat oleh tingginya mortalitas benih karena rendahnya resistensi terhadap penyakit. Resistensi merupakan sifat yang diwariskan dari tetua ke anakan. Benih yang resisten dapat diseleksimenggunakan marka molekuler seperti gen Major Histocompatibility complex (MHC). Sifat resisten diduga berbeda diantara benih yang beraasal dari induk berbeda dan diduga terkait dengan kompenen genetik yang dimiliki. Tujuan penelitian ini adalah untuk mengetahui sifat reisten dan karakter molekuler benih gurami sowang asal induk berbeda. Fragmen gen MHC diamplifikasimenggunakan teknik PCR. Sifat resistensi dianalisis menggunakan perhitungan matematika sederhana berdasarkan jumlah benih hidup dibagi jumlah total benih yang diinfeksi, sedangkan karakter molekuler dianalisis secara deskritif berdasakan pola pita yang dihasilkan. Kedua kelompok benih memiliki sifat resistensi berbeda terhadap Aeromonas hydrophila. Benih dari induk pertama memiliki sifat resistensi lebih rendah dengan nilai kelangsungan hidup sebesar 29% daripada kelompok benih dari induk kedua yang memiliki kelangsungan hidup mencapai 100%. Kedua kelompok benih juga memperlihatkan perbedaan sifat resisten terhadap P. aeruginosa, meskipun perbedaannya tidak sebesar terhadap A.hydrophila. Perbedaan tersebut juga tercermin pada gen MHC kedua kelompok benih. Pada kelompok benih pertama dihasilkan dua fragmen gen MHC dengan ukuran 585 pb dan 400 pb, sedangkan dari kelompok benih kedua hanya dihasilkan fragmen berukeuran 400 pb. Perbedaan pola pita diantara kedua kelompok benih tersebut merupakan idikasi adanya perbedaan karakter molekuler pada benih yang berasal dari dua induk berbeda

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