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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general.
Arjuna Subject : Umum - Umum
Articles 14 Documents
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Search results for , issue "Vol. 9 No. 2 (2022)" : 14 Documents clear
ANTIOXIDANT EFFECT OF MORINGA (Moringa oleifera) LEAF ETHANOL EXTRACT ON SPERMATOGENESIS IN OLD WISTAR RATS (Rattus norvegicus) Luh Putu Widiastini
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Infertility is the failure of pregnancy after regular sexual intercourse for 6-12 months without contraception. Infertility in man is primarily caused by damaged sperm production, for example, impairments in the spermatogenesis process, low spermatozoa concentrations, morphological factors, and abnormal sperm motility. The purpose of this study is to ascertain the antioxidant effect of moringa (Moringa oleifera) leaves ethanol extract on spermatogenesis (spermatogonia, spermatocytes, and spermatids counts) in old Wistar rats (Rattus norvegicus). This study took healthy old Wistar rats aged 18-19 months with a body weight of 200-250 g and with no physical disabilities. A total of 36 was Wistar rats divided into two groups, namely the treatment group (supplied with Moringa leaf ethanol extract of 50 mg/kgBW/ and 0.5% Carboxymethyl cellulose (CMC) of 0.5 mL per day) and the control group (only with 0.5% CMC of 0.5 mL per day) for 30 days. The results showed that there was a significant difference in the spermatogonia, spermatocyte, and spermatid counts between the group treated with Moringa leaf ethanol extract and the control group, resulting in a p-value of 0.000. Therefore, it can be concluded that Moringa leaf ethanol extract had a significant influence on the spermatogonia, spermatocyte, and spermatid counts in old Wistar rats.
MICROPROPAGATION OF POTATO (Solanum tuberosum L.) cv. GRANOLA IN LIQUID MEDIUM USING AERATION SYSTEM FOR G0 SEED PRODUCTION Karyanti
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Disease-free potato seeds of high quality can be obtained via in vitro culture. The use of liquid medium during in vitro cultures might boost the number of plantlets produced, however, the problem of hyperhydricity in plantlets was often encountered. This study aimed to investigate effects of different sucrose concentrations and application of aeration system on micropropagation of potato cv. Granola using liquid medium. Aseptic nodal explants with 3-4 nodes from established in vitro cultures were subjected to MS liquid medium with a factorial treatment of three sucrose concentrations (0, 7.5, 15 g. L-1) and two culture (with and without aeration). The results showed that MS medium with 7.5 g L-1 sucrose was the best medium to produce the highest number of shoots and nodes. Furthermore, it was found that application of aeration system in MS liquid culture decreased plantlet hyperhydricity and increased the number of shoots, number nodes, plantlet height, as well as improved plantlet morphology and vigor. Application of the aeration system in liquid medium produced 200-230 new potato plants per bioreactor in the acclimatization stage and an average of 2773.5 G0 mini tubers.
APPLICATION OF RECOMBINANT TRIACYLGLYCEROL LIPASE AND CARBOXYLESTERASE ENZYMES FROM Bacillus velezensis STRAIN S3 FOR POLYESTER SURFACE MODIFICATION Is Helianti
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Enzymatic polyester surface modification can be performed with lipase and esterase enzymes. In this study, the polyester fabric modification utilized triacylglycerol lipase (TGA) and carboxylesterase (CES) recombinant enzymes. The effect of these treatments was observed by determining the hydrophilicity level, dye absorption level, hydroxyl group measurement, and fiber surface morphology. The results revealed an elevated hydrophilicity level in polyester fabric, followed by dye absorption improvement and carboxyl group increase. The water absorption times required by the fabric based on the results of TGA, CES, comparative lipase, and negative control treatments were 3±0.05 seconds, 3.5±0.07 seconds, 5±0.05 seconds, and 80±11.54 minutes, respectively. Dye absorption test in polyester fabric based on these groups mentioned above were 52±0.5, 58±0.5, 178±0.5, and 2968±290 seconds. The total hydroxyl group measurement in polyester fabric was observed at 30.9±0.09, 30.5±0.05, 28.6±0.09, and 3 meq/100 g. The SEM observation showed that the enzymatic hydrolysis could alter the porous structure and surface of the fibers.
TOTAL LIGNIN CONTENT AND AGROMORPHOLOGICAL CHARACTER DIVERSITIES OF 30 INDONESIAN RICE (Oryza sativa L.) ACCESSIONS Satya Nugroho
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Lignin is one of lignocellulosic components in vascular plants, essential in plant mechanical properties, water transport, and defense against pathogens. Furthemore, lignin has been applied in various industry. This study aimed to explore variation of lignin content and its-related morphological traits of Indonesian rice accessions, providing beneficial information for breeding approaches to improve utilization characteristics of grass biomass. Therefore, the total lignin contents of thirty Indonesian rice accessions using Thioglycolic Acid Lignin (TGAL) method and their correlation to seven agromorphological characters using Pearson correlation analysis were investigated. Variation of lignin content ranged from 8.38 to 20.75% (of cell wal residue, CWR), and the average value was 13.55%. Correlation analysis showed that lignin total positively correlated with plant height, stem length, panicle length, stem diameter, total fresh weight, and panicle weight per tiller. On the other hand, the number of tillers had a significantly negative correlation to lignin contents.
OPTIMIZATION OF Bacillus paramycoides FERMENTATION MEDIUM TO INCREASE THE PRODUCTION OF 5-AMINOLEVULINIC ACID IN A 10 LITER FERMENTER Dicky Adihayyu Monconegoro
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

5-Aminolevulinic acid is an essential precursor for the biosynthesis of tetrapyrrole compounds, such as chlorophyll and heme. 5-ALA has the potential to be used as a plant growth and antioxidant activity enhancer. 5-ALA can be produced through fermentation by Bacillus paramycoides. This study aimed to optimize B. paramycoides fermentation medium to increase 5-ALA production. The optimization was carried out using response surface method (RSM) experimental design. 5-ALA production in a 10 L fermenter was conducted using an optimized medium and supplemented with MSG as a precursor and wood vinegar as an inhibitor. The results showed that the best medium composition was 27.78 g L-1 molasses; 9.145 g L-1 urea; 8.838 g L-1 NaCl; and 32.07 g L-1 glucose, resulting in 10.749 (log CFU mL-1) and 255.30 µM 5-ALA. 5-ALA production trial in a 10 L fermenter produced 581.82 µM 5-ALA. Medium optimization and precursor-inhibitors addition in the fermentation increased the 5-ALA yield 3.2 times compared to before optimization.
ANTIBACTERIAL ACTIVITY OF Chaetoceros calcitrans AGAINST PATHOGEN Staphylococcus aureus AND Staphylococcus epidermidis CAUSING SKIN INFECTION Patricia Gita Naully
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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The microalgae Chaetoceros calcitrans has potential as a natural antibacterial but is rarely applied to pathogens that cause skin infections such as Staphylococcus aureus and S. epidermidis. The purpose of this study was to determine the optimum concentration of C. calcitrans extract to inhibit the growth of S. aureus and S. epidermidis. The antibacterial activity of C. calcitrans was tested by the Kirby Bauer diffusion method. The results showed that C. calcitrans extract dissolved in DMSO with the concentrations of 5, 10, 15, and 25 mg mL-1 could produce inhibition zones on S. aureus and S. epidermidis. The average diameter of the largest inhibition zone resulted in the concentration of 25 mg mL-1, namely 10.1 ± 0.5 mm in S. aureus and 9.3 ± 0.5 mm in S. epidermidis. It can be concluded that the extract of C. calcitrans has antibacterial activity against bacteria that cause skin infections S. aureus and S. epidermidis with the optimum concentration of 25 mg mL-1.
THE EFFECT OF LIQUID TOFU WASTE AND GROWING MEDIA ON THE GROWTH AND YIELD OF LONG BEANS (Vigna sinensis L.) Nindi Fauziah
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Long bean is a leguminous plant that has the potential to be developed into productive plants. Tofu liquid waste organic fertilizer and planting media are needed to meet nutrient needs, improve the physical and chemical properties of the soil. This study aims to understand the effect of liquid tofu waste, growing media, and their combined interaction on the growth and yield of long beans. This study used a factorial randomized block design (RBD) with 3 replications, 3 treatments of liquid tofu waste of 0 mL (T0), 150 mL (T1), and 300 mL (T2), and 3 planting media, namely cow manure compost + rice husk charcoal (M1), cow manure compost + cocopeat (M2), and cow manure compost + chopped fern (M3). If they had a significant effect, a Tukey's test at a 5% probability level would be performed. The parameters observed included the chlorophyll a and b, net assimilation rate, relative growth rate, height, number of flowers, pod length, and wet fruit weight of long beans. The results showed that the T1 and M3 treatments significantly affected chlorophyll a and b, height, number of flower, and pod length. The T1 and M2 treatments significantly affected the wet fruit weight of long beans.The T2 and M2 treatments significantly affected the net assimilation rate. Finally, the T2 and M3 treatments significantly affected the relative growth rate. There was no interaction effect found between liquid tofu waste and growing media on all research parameters.
MORPHOLOGICAL AND TEXTURAL FEATURE EXTRACTIONS FROM FUNGI IMAGES FOR DEVELOPMENT OF AUTOMATED MORPHOLOGY-BASED FUNGI IDENTIFICATION SYSTEM R. Putri Ayu Pramesti
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Due to widely varied microscopic shapes, fungal classification can be performed based on their morphological features. In morphology-based identification process, feature extraction takes an important role to characterize each fungal type. Previous studies used feature extraction of fungal images to detect the presence of fungal. In this study, morphological and textural features were extracted to classify three types of fungi: Aspergillus, Cladosporium and Trichoderma. Geometry and moment were used as morphological features. To perform textural feature extraction, the local binary pattern (LBP) and gray level co-occurrence matrix (GLCM) feature extraction method were used. We compared the implemented feature extraction methods in order to get the best classification result. The result showed that geometrical features has the accuracy of 65%, higher than that of LBP (60%), GLCM (45%), and moment accuracy (55%). This suggested that geometric features is important for fungal classification based on their morphology.
EFFECTS OF SEED COATING USING PLANT-BENEFICIAL MICROBES ON THE GERMINATION OF Centrosema pubescens SEEDS Indri Handayani
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Centrosema pubescens is a legume plant that is commonly used as animal feed, cover crop, and one of the plants used to reclaim critical land. This study was conducted to increase the germination of C. pubescens seeds by coating the seeds using a coating material enriched with a consortium of microbes consisting of nitrogen-fixing bacteria, phytohormones-producing bacteria, phosphate-solubilizing bacteria, and arbuscular mycorrhizal fungi. Germination test on control, seed coating, and microbial seed coating was carried out using the top of the sand method for 28 days. Observational data were tested using ANOVA statistics and LSD tests. Results of the research showed the moisture content of the seeds was 12.45%, the purity of the seeds was 95.11% and the weight of 1000 seeds was 23.74 g. The germination test denoted that the number of normal germinated seeds in seed coating and microbial seed coating was significantly different from the control. In addition, dead seeds in microbial seed coating treatment had the lowest value and were significantly different from other treatments. These results indicate that microbial seed coating can increase seed germination and considerably reduce seed death due to seed-borne pathogens.
PRODUCTION OF HUMAN INSULIN PRECURSOR IN A 10 LITER BIOREACTOR USING A METHANOL PULSE FEEDING Dian Japany Puspitasari
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Indonesia as a country that had 19.5 million diabetic patients in 2021 is still importing raw materials of human insulin. The development of human insulin production process is needed to support the government’s objective to be independent in medicine aspect. Human insulin precursor (HIP) expressed from Pichia pastoris X33/pD902-IP had been developed and optimized in small-scale cultivation. However, the scaling up in bioreactor 10 L has not been studied. Using a 10 L-bioreactor the fermentation of P. pastoris X33/pD902-IP was developed. Fermentation was done in 120 hours using a basal salt medium (half concentration) for the vegetative and induction media. To induce HIP expression, methanol is fed by pulse strategy with a gradient concentration of 1-3% (v/v) for 48 hours. The dry cell weight (DCW) and HIP titers were 72 g/L and 286 mg/L, respectively. This development is the first fermentation of HIP expressed by P. pastoris X33/pD902-IP in a 10 L-bioreactor.

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