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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general.
Arjuna Subject : Umum - Umum
Articles 14 Documents
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Search results for , issue "Vol. 9 No. 2 (2022)" : 14 Documents clear
VOLATILE COMPOUND ANALYSIS OF AROMATIC RICE MUTANT LINES USING HS-SPME/GC-MS Muh Riadi
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Volatile compound is one of the key factors for aromatic components of rice. This study aimed to identify the key aroma components and their relationship with plant productivity in the Pare Bau variety mutant lines and its wild type. Volatile extraction was carried out using the headspace solid-phase microextraction (HS-SPME) method and analysed by the Gas Chromatography-Mass Spectroscopy (GC-MS) instrument. The results of the identification of volatile compounds showed a total of 224 compounds in the mutant lines and wild type. However, only 14 compounds were suspected as key aroma compounds in Pare Bau rice, namely 2-Acetyl-1-pyrroline, Indole, 1-Octanol, 1-Octen-3-ol, 2,4-Nonadienal, (E,E)-, Octanal, 2-Nonenal, (E)-, 2-Octenal, (E)-, Decanal, Hexanal, Nonanal, Furan, 2-pentyl-, toluene and vanillin. The results on aroma compounds of mutant lines using principal component analysis showed that there were differences in the main characteristics of several strains and wild type. There was no relationship between increasing volatile concentration and plant productivity, vice versa.
UTILIZATION OF SAGO DREGS AS RUMINANT FEED BY USING THE FERMENTATION METHOD: LITERATURE REVIEW Titi Lahanda Susanti
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Every year there is a change in the stock of ruminant feed that occurs in the rainy season and water shortages in the dry season. Utilization of agricultural waste as an alternative feed is one way to overcome these problems. One of the wastes that have the potential to be used as feed ingredients is sago waste. Sago pulp is a waste that is rich in lignocellulose, namely cellulose. Several biotechnology applications in ruminant feed fermentation can improve properties such as taste, aroma, shelf life, texture and nutritional value of food. Fermentation using mold or yeast, as well as bacteria or a mixture of various microorganisms can increase the nutrients in the feed needed by ruminant feed. Processing of lignocellulosic materials is required to obtain optimal degradation results. The degradation process will convert lignocellulosic material into raw materials that are easily digested by the ruminant. Enzymes produced by microorganisms can increase crude protein, crude fat, carbohydrates, crude fiber, vitamins and minerals. Thus, the fermentation method of sago dregs and its use as feed can increase the nutritional value, so that productivity can be increased when given as feed.
REVIEW: ANIMAL SERUM REPLACEMENT IN MESENCHYMAL STEM CELLS CULTURE Ariyani Noviantari
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Mesenchymal stem cells (MSCs) are being used in clinical applications and must comply with Good Manufacturing Practices (GMP) standards and The National Agency of Drug and Food Control (NA-DFC)) regulations. MSCs cultured using a culture medium and added with several supplements like animal serums. However, animal serums can be a source of virus transmission. Therefore, it is necessary to substitute supplements for the animal serum that are safe to use in cell therapy using MSCs. The paper discusses substituting animal serum MSCs culture. This paper is a literature review through a literature search in scientific journals and research reports that explains the various studies on free serum in the culture of MSCs. It can be concluded that human platelet lysate (hPL), human platelet-rich plasma (hPRP), human serum (hS), human umbilical cord serum/plasma (hUCS/P), or human plasma-derived supplement for cell culture medium (SCC) can be used as substitutes for animal serum in MSCs culture.
INCREASING RECOMBINANT PENICILLIN G ACYLASE PRODUCTION: GENETIC, PROTEIN ENGINEERING, AND PRODUCTIVITY IMPROVEMENT Is Helianti
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 2 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

B-lactam derived antibiotics are the most used globally for treatment against different infections caused by pathogenic bacteria and comprises 65% of the world antibiotics. Recently, penicillin G acylase (PGA) is used as biocatalyst for those B-lactam antibiotics production by which 6-aminopenicillanic acid (6-APA) or 7-aminodeacetoxycephalosporanic acid (7-ADCA) as the building blocks is produced. Commercialized PGA from native microbial resources are still limited to E. coli. Therefore, genetic engineering approach such as cloning and expression in other microbial hosts were assessed to enhance bacterial strains that produce PGA. However, such improvement could increase immature precursors accumulation and lowering the enzyme yield, activity, or stability. This review focus on the review of PGA recombinant produced by several microbial host, their expression levels, and improvement achieved by some modification such as replacement of signal peptide and promoter continued to protein engineering to utilize the enzymes in synthetizing amoxicillin rather than to hydrolyses Penicillin G.

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