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Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 10 Documents
 1 
Search results for , issue "Vol. 5 No. 3 Juli 2016" : 10 Documents clear
PENAPISAN DAN PEMANFAATAN RHIZOBAKTERI TANAMAN JAGUNG (Zea mays) SEBAGAI INOKULAN PEMACU TUMBUH TANAMAN Debby Widiyanti; Sri Pujiyanto; Agung Suprihadi; Mamik Setyowati
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Maize (Zea mays) is the second most important food commodity after rice plants. The role of the corn crop in agricultural commodities encourage the use of biological fertilizers to increase crop productivity of maize. The use of biological fertilizers open opportunities using rhizosphere bacteria as inoculants boosters grow plants.This study aims to obtain bacterial isolates from the rhizosphere of maize (Zea mays), characterize the morphology and biochemical properties of the ability to produce IAA, provide nutrients such as N and P and to get a bacterial inoculant boosters grow corn. Rhizosphere bacteria were isolated using SEA media (soil extract agar). Isolates that have the ability to produce IAA, tether N and high P solvents tested against corn seed germination and applied in a phase of plant growth. The results showed there were 24 isolates corn crop rhizosphere dominated by gram-positive bacteria and shaped basil capable of producing IAA grading 4,83 to 125,84 ppm. 16 rhizosphere bacterial isolates capable of dissolving phosphate with phosphate solubility index ranged from 2,1 to 4,6. 18 rhizosphere bacterial isolates capable of tether N with the highest N tether capacity of 2,8 x 10 3 CFU/ mL. Application 3 selected bacteria such as J11, J16 and J19 as inoculants are not able to increase the growth of corn plants compared to control water. Keyword :Maize, Rhizosphere, The growth of corn plants.
PENURUNAN KADAR AMONIA FESES AYAM PEDAGING MENGGUNAKAN PREBIOTIK BUNGKIL INTI SAWIT DENGAN INOKULUM BAKTERI Lactobacillus acidophilus, Lactobacillus bulgaricus, DAN Bacillus cereus Ridho Mathori Ikhwan; MG. Isworo Rukmi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Ammonia emissions at the farm that was from the broiler faecal waste inflict restlessness and become the source of respiratory diseases to the local people because the farm is located adjacent to residential areas. Bacterial isolates were characterized as acid producer with BIS prebiotic mixture may be a bioremediation agent to reduce ammonia emissions in broiler feces. The purpose of this research is to create a biotechnology product which is ready to apply for reducing the ammonia at the poultry farm.  As the treatment used three isolates of bacteria such as L. acidophilus, L. bulgaricus and B. cereus were each used to ferment the BIS for 48 hours at a temperature of 38 ° C. Results of subsequent fermentation inoculated into the stool with a concentration of 8% (w / w), and incubated for 48 hours. Observations ammonia, pH and microflora in the stool, were done at 24 and 48 h of incubation. The results showed a decrease in the levels of ammonia and pH were significantly different between the control and all treatments, but there is no real difference between each treatment of bacterial isolates. Decreased levels of ammonia at 24 h incubation by L. acidophilus, L. bulgaricus and B. cereus, respectively for 92.89%, 98.78%, 98.56%, while the 48-hour incubation, respectively for 90.67 %, 89.60% and 96.80%.Keywords; Ammonia, Acid Producing Bacteria, Prebiotics, BIS, Broiler
DAYA AKUMULASI LOGAM BERAT TEMBAGA (Cu) PADA AKAR DAN DAUN Avicennia marina (Forsk.) BERDASARKAN FASE PERTUMBUHAN YANG BERBEDA DI PANTAI MANGKANG SEMARANG Ahmad Fadhli Jundana; Endah Dwi Hastuti; Rini Budi Hastuti
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Distribution of heavy metals in coastal areas need to know as an early indication of pollution. One type of metal that was widely used in household and industrial activity is copper (Cu). Some pollutants were wasted on the ground will have accumulated in coastal areas, including in the mangrove ecosystem. The research aims to determine the accumulated rate Cu in roots and leaves of Avicennia marina by different growth phases. It was conducted in mangrove ecosystems Mangkang, Semarang. The research design was factorial completely randomized design 2 x 3, the first factor were organs (roots and leaves) and the second factor were phase (Seedling, Sapling and Trees) with twice of  sampling in a 30-day period, the data were analyzed by Analyzed of Variates (ANOVA). Parameter of the research were Cu metal content in the roots and leaves of mangrove, as well as water and sediment. The results showed that the levels of Cu in sediments and aquatic environments of Avicennia marina rangely 2.989-7.026 mg/kg and 0.186-1.676 mg/l, there was an interaction between the growth phase and organs Avicennia marina in accumulating Cu with a probability of 0.006 (< 0.05), the highest interaction was the tree phase and root organ section (0.126) and the lowest was the seedling phase and leaf organ (0.020). The highest of Biological Concentration Factor (BCF) value was tree phase and root organ (92.9- 46.2) and the lowest was the seedling phase and leaf organ (17.2-14.7). The highest of Distribution Coefficient (DC) value was found in seedling phase environment (726.3-1887.7) and the lowest was found in sapling phase environment (493.2-603.4).Key Words : Accumulation, Avicennia marina (forks.), Heavy Metal Cu, Biological Concentration Factor, Distribution Coefficient
AKTIVITAS INHIBITOR α-GLUKOSIDASE ISOLAT KAPANG ENDOFIT DUWET (Syzygium cumini (L.) Skeels) Roseliana Fitri; Agung Suprihadi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Java plum known to have the ability as a diabetes drug. The essence of diabetes treatment is to maintain blood sugar levels in normal state using α-glucosidase inhibitor. Such capabilities can be obtained from endophytic mould found in java plum plant. The aim of this study is to get the best isolates that have the ability of α-glucosidase inhibitors with different carbon sources. This study uses java plum endophytic mould isolates which tested the ability of α-glucosidase inhibitor by using spectrophotometric techniques with breaking substrates to produce colored products and a variety of carbon sources such as sucrose, lactose, maltose and starch. The results of α-glucosidase inhibitor activity indicate that crude extract of isolates JB 2.3 has the best ability to produce α-glucosidase inhibitor with a percentage of 13.76% is higher than the positive control acarbose 1%. Lactose be the best carbon source with a mean inhibition of (19.27%), sucrose (13.58%), starch (12.70%), Maltose (10.76%).Keywords: α-glucosidase inhibitor, endophytic mould, java plum, diabetes melitus.
PERBAIKAN HISTOLOGIS PUSAT LEARNING AND MEMORY DI HIPOKAMPUS OTAK SETELAH PERLAKUAN SUPLEMEN DAGING IKAN GABUS (CHANNA STRIATA) DALAM PAKAN: KAJIAN IN VIVO PADA TIKUS WISTAR PASCA STRES Reynato Wijaya Saputra; S Sunarno; Siti Muflichatun Mardiati
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Background: Stress due to lack of feed that followed excessive activity is a trigger factor learning and memory disorder center in the hippocampus. Cornu ammonis (CA3 and CA1) in the hippocampus is a structure that is central to learning and memory. Complete nutrient content of feed is supporting the regeneration of the structure of CA3 and CA1 in the hippocampus impaired due to stress . This research was conducted to obtain a histological improvement layers of CA3 and CA1 hippocampus in the rat brain after supplementation with snakehead fish meat in the  feed of post-stress.Design and Method: This study uses a completely randomized design consisting of                 6 treatments with 4 replications. Test animals used were male Wistar rats. Treatments, including P0: control, namely Wistar rats were conditioned to stress during the six days that followed the feeding without supplementation of snakehead fish meat for 14 days; P1, P2, P3, and P4, respectively Wistar rats were conditioned stress followed by supplemental feeding snakehead fish meat 5%, 10%, 15% and 20%. The research variables measured the thickness of the layer of CA3 and CA1. Data were analyzed using Analysis of Variance (ANOVA) at the level of 95% and continued with Duncan test with 95% significance level .Result: Supplemental feeding with snakehead fish meat as much as 15% during 14 days in Wistar rats after stress gives the best effect on the improvement of CA3 and CA1 layer of the hippocampus, respectively 53.60 μm2 (17.49%) and 46.31 μm2 (78,32%), better than the control.Conclusion: Feeding the snake head fish meat supplementation for 14 days in Wistar rats after stress can improve layer CA3 and CA1 in the hippocampus of the brain. Recommended snake head fish meat can be used as a feed supplement to improve the structure of CA3 and CA1 in the hippocampus and very good to support the learning and memory functions of the brain. Keywords: snakehead fish meat, Channa striata, cornu ammonis, supplementation, hippocampus, learning and memory
Pengaruh Kombinasi Pupuk daun dan Nano silika terhadap Pertumbuhan Anggrek (Dendrobium sp.) pada Subkultur secara In Vitro Imroatul Khasanah; Erma Prihastanti; Endah Dwi Hastuti; Agus Subagio
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Orchid is one of the horticulture commodities that are very important in international trade. The problem that is commonly found in the plant tissue culture is hyperhydricity which causes the growth of plant to be too slow. Which is also a problem in acclimatization. Leaf fertilizers contain macro and micro nutrient that promote plant growth. Nano silica is a nutrient which is needed by monocotyl plants to support growth and prevent deficiency or poisoned nutrient. The aim of this research is to know the effects the combination of leaves fertilizer with nano silica and know optimal concentration of  leaves fertilizer and nano silica combination to growth of orchids (Dendrobiumsp) in subculture. This research used Completely Randomize Factorial Design (CRFD) (4x2) with four replication. First factor is leaves fertilizer (G) consists of four extent that is G0: 0,000 g/l; G1: 0,250 g/l; G2: 0,375 g/l; G3: 0,500 g/l. Second factor is nano silica (N) that consists of two extent that is N0: 0 ppm; N1: 20 ppm. The parameters which were measured in this research are number of leaves, plant height, number of roots, and the number of shoots. The data were analyzed by Analysis Of Variance (ANOVA) and continued with Duncan’s Multiple Range Test (DMRT) in extent 95%. The results of this research show that there are effects of interaction between leaves fertilizer and nano silica to the amount of roots. Leaves fertilizer does not really affect the orchid but it tends to increase plant height, the amount of shoots and orchid performance. Nano silica does not really affect the orchid but it tends to increase the amount of leaves, plant height and orchid performance. Keyword: Growth, subculture, concentration, leaves fertilizer, nano silica
KARAKTERISASI MOLEKULER TANAMAN SAMBUNG NYAWA (Gynura procumbens [Lour.] Merr) BERDASARKAN 18S rRNA Octavia Dewi Christiningrum; Anto Budiharjo; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Sambung Nyawa (Gynura procumbens) is a traditional medicinal plant used by people as an antihyperglycemic drug, antihyperlipidemic and chemoprevention agents. Comprehensive information on this plant needs to be done, including DNA analysis, so it can complement morphological character of an organisms. This study aimed to molecularly characterize and determine the relationship between G. procumbens with other organisms using ribosomal 18S rRNA gene. PCR (Polymerase Chain Reaction) was used to amplify the 18S rRNA gene fragments G. procumbens. DNA sequences were analyzed using the BLAST program at the NCBI portal and construction of phylogenetic trees were tested using Bootstrap method 1000x. The results showed the 18S rRNA sequence of Gynura procumbens fragments was 1600 bp, and G. procumbens belonged in the family of Asteraceae.Key words : Gynura procumbens, PCR (Polymerase Chain Raction), 18S rRNA, Bootstrap method
IDENTIFIKASI KANDUNGAN SENYAWA FITOKIMIA MINYAK BIJI MIMBA (Azadirachta Indica, A. Juss) Dyah Palupi; Endang Kusdiyantini; Rully Rahadian; A Heru Prianto
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Neem seeds oil (Azadirachta indica, A. Juss) have phytochemical compounds which act as antifeedant, repellent, antibacterial, and antifungal. The neem seeds oil were important identified their phytochemical compounds. Identifications were done with screening phytochemical and infrared spectrophotometer. Neem seed oils were obatained with pressing method. The group of compounds tested were alkaloid, flavonoid, triterpenoid, steroid, saponin and tannin. The results of phytochemical showed that neem seeds oil consist of alkaloid, flavonoid, triterpenoid, steroid, saponin and tannin. This results were strengthned with spectrum in infrared spectrophotometer that showed the functional groups of each those compounds. Keywords: neem seeds oil, phytochemical compounds, pressing method, functional groups
PENGARUH NAUNGAN MENGGUNAKAN PARANET TERHADAP PERTUMBUHAN SERTA KANDUNGAN KLOROFIL DAN β KAROTEN PADA KANGKUNG DARAT(Ipomoea reptans Poir) Ika Wulandari; Sri Haryanti; Munifatul Izzati
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Kangkung is one of the of the most favourite vegetable of Indonesian people. Most of indonesian including low-class to high-class like to consume it. Kangkung is often coocked as many different kind of dish such as, gado-gado, tumis kangkung, sayur bening, pecel. That is the proof from social side that Indonesian people can accept kangkung to become daily consumption. The purpose of this research is to analyze the effect of shade on growth, chlorophyll, carotenoid content of kangkung. The Parameters were plant height, number of leaf, root length, fresh weight of plant, number of stomata, chlorophyl content and β carotene content. This research was conducted at the Laboratory of the BSF Plant of Department of Biology Undip FSM. This research ks designed using shade as one factor RAL. Data analysis was using ANOVA at significance level of 95%, if there is significant difference than continued with Duncan Multiple Range Test (DMRT). The results show that different shade makes no significant different on plant height, number of leaf, fresh weight of plant, number of stomata, β carotene content but tend to increase yet decrease chlorophyll content. Using shade with two layers of paranet result in the lowest of chlorophyl content, but the most of β carotene content if compared to the others treatment.Keyword : shade, kangkung, chlorophyll, β carotene
ISOLASI BAKTERIOFAG SPESIFIK Pseudomonas sp. DA1 DARI BIOFILM PADA SISTEM PENGISIAN AIR MINUM ISI ULANG. Zikra Hayati; Siti Nur Jannah; Agung Suprihadi
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The social demand for drinking water increases both in urban and rural areas. The state encourages the development of Industrial Water Supply Depot (DAM) refills. problems occurred is no standard method for treating drinking water is sterileand lack of government supervision. This raises the issue of sanitation that is the presence of pathogenic bacteria that form biofilms in drinking water refill system. One of the bacterial pathogen is Pseudomonas. Bacteria Pseudomonas at refill drinking water can cause infections in the digestive tract. This study aims to find specific bacteriophage isolates of biofilm samples to infect bacteria Pseudomonas sp. on refill drinking water system. Isolation host Pseudomonas done in 2 ways dilution and filtration and grown on selective media Pseudomonas Isolation Agar. Isolates obtained were then characterized by gram staining and Kligler Iron Agar (KIA) test. Isolation of bacteriophages of biofilm done by bacteriophage amplification and filtration to obtain filtrate bacteriophage I and II. Test performed by using the host's infection Pseudomonas sp. DA1, Pseudomonas aeruginosa and Salmonella sp. (LIPI’s collection). The results showed that the drinking water refill depot contained positive isolates of Pseudomonas is Pseudomonas sp. DA1 and obtained isolate specific bakteriofag that can infect Pseudomonas sp. DA1. Bacteriophage titer calculation results on each sample at 3,0 x 105 PFU/ml in drinking water depot, 3,3 x 107 PFU/ml in the water product, and 9 x 107 PFU/ml water sources. Keyword: refill drinking water systems, biofilms, Pseudomonas sp DA1, bacteriophage

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