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Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 11 Documents
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Search results for , issue "Vol. 6 No. 3 Juli 2017" : 11 Documents clear
KERAGAMAN BAKTERI ASAM LAKTAT SECARA MOLEKULER PADA ILEUM DAN SEKUM AYAM BROILER YANG DIBERI PAKAN PREBIOTIK BEKATUL DAN BEKATUL HASIL FERMENTASI Laelatul Baniyah; Siti Nur Jannah; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Lactic Acid Bacteria (LAB) is a digestive tract microflora that play a positive role in poultry health. The number and diversity of LAB in the digestive tract affected by several factors, among them was the kind of feed. The purpose of this research was  to know the molecular diversity of Lactic Acid Bacteria (LAB) in broiler’s ileum and cecum  after  feeding with  prebiotic bran and Rhizopus oryzae fermented  bran which was added to commercial feed. The molecular analysis was done using T-RFLP method , Hae III and Msp I were used as restriction enzymes.  The number of phylotype, relative abundance, Shannon diversity index (H '), evenness (E), and Dominance (D)  were examined . The results indicated that the addition of bran prebiotics on commercial feed showed a higher  diversity of lactic acid bacteria on broiler’s ileum and cecum, compared  with the addition of Rhizopus oryzae fermented  bran. The dominant BAL types are Lactobacillus spp, L. delbrueckii subs. Bulgaricus, L. intermedius, L. amilovorus, uncultured bacteria 87 bp, 280 bp, 331 bp and unidentified bacteria 74 bp, 82 bp, 131 bp. Keywords: Diversity, Lactic Acid Bacteria, Prebiotics, Bran, T-RFLP
DETEKSI GEN tlh DAN tdh PADA Bakteri Vibrio parahaemolyticus DARI AIR TAMBAK UDANG VANNAME (Litopenaeus vannamei) DI KABUPATEN REMBANG Adila Nawan Hasrimi; Anto Budiharjo; Siti Nur Jannah
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Vibrio parahaemolyticus is hallophilic gram-negative bacteria that live as natural inhabitant in aquatic environment. All Vibrio parahaemolyticus strain known to have thermolabile hemolysin encoded by tlh gene as species marker. Thermostable direct hemolysin encoded by tdh gene is responsible for regulating one of the virulence factors in Vibrio parhaemolyticus. The aim of this research is to detect tlh gene and tdh gene from water of vanname shrimp’s aquaculture in Rembang regency. Colonies of green-blueish bacteria grew from the isolation of  vanname shrimp’s aquaculture water in CD-VP media which is identified as Vibrio parahaemolyticus. The isolated bacteria is specifically identified as Vibrio parahaemolyticus bacteria by the detection of tlh gene. Molecular analysis shows tdh negative result that indicates tdh gene is not present in the isolated bacteria. Vibrio parahaemolyticus isolate were cultured in Wagatsuma agar for the tdh gene confirmation test that showed Kanagawa negative result, in which indicated that V. parahaemolyticus did not produce thermostable direct hemolysin. Vibrio parahaemolyticus isolate did not show any virulence factors to initiate host colonization in the aquatic environment. Keywords: Vibrio parahaemolyticus, tdh gene, tlh gene
IDENTIFIKASI SENYAWA BIOAKTIF PADA ISOLAT BAKTERI BUAH BELIMBING WULUH (Averrhoa bilimbi L.) SEBAGAI AGENSIA HAYATI Xanthomonas oryzae pv. oryzae Aniza Rachmawati; Agung Suprihadi; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bilimbi (Averrhoa bilimbi L.) is one of original fruit plants from Indonesia. Bilimbi fruits contain flavonoid, saponin, triterpenoid and tanin that have ability as anti-microbial. Bacteria was isolated from bilimbi fruits expected to be able produce bioactive compounds which can kill pathogenic bacteria such as Xanthomonas oryzae pv. oryzae which caused Bacterial Leaf Blight (BLB). Bacterial Leaf Blight in Indonesia caused harvest losses of 18 – 36 %. Infection was caused by X. oryzae caused the leaf symptoms to turn pale yellow, white, withered, and finally die. The purpose of this research are get bacteria isolate from bilimbi fruits (Averrhoa bilimbi L.) and testing bioactive compounds on bacteria isolate from bilimbi fruits which can inhibit Xanthomonas oryzae pv. oryzae (Xoo) growth. This research use methods, that are isolation of bacteria from bilimbi fruits and isolation of Xanthomonas oryzae pv. oryzae, characterization biochemically, inhibition test, and Thin Layer Chromatography. Obtained two bacteria isolate from bilimbi fruits. They are IBW1 dan IBW2, inhibition zona of IBW1 is 0,15 mm and IBW2 0,35 mm. Both of them have potential in antibacteria of X. oryzae bacteria eventhough in weak catagory. Metabolite secondary compound which play a role in antibacteria of X. oryzae is flavonoid compound. Kata kunci: antibacteria, bilimbi, Thin Layer Chromatography, X. oryzae
KEANEKARAGAMAN MOLUSKA TERESTRIAL DI JALUR PENDAKIAN SELO TAMAN NASIONAL GUNUNG MERBABU, KABUPATEN BOYOLALI, JAWA TENGAH Larosi Nufikri Garmellia; Jafron Wasiq Hidayat; Fuad Muhammad
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Terrestrial mollusc is one of the most important basic component in a terrestrial ecosystem and has an important role in forest ecosystem. Ecologically, they could be an indicator to determine the habitat.This research located in Taman Nasional Gunung Merbabu especially Selo Track give some informations for biodiversity and conservation purpose. This research was conducted on November 2016 until January 2017. For research method, it usedpurposive sampling method for select survey location and stratified random sampling with diagonal plot area of 10m x 10m for sampling method. The data analysis used Shannon-Wienner diversity index (H’) and evenness index (e).Results found the total species obtained 43 species of 11 Famili. The most Common speciesfound were, Diplommatina perpusilla, Helicarion albellus, Microcystina exigua and Landouria smironensis. Diversity index (H') ranged from 0.75-2.57whichcategorized for low to moderate. Evenness index value (e) range 0,41-0,77 whichcategorized for  moderate to high.  Keyword:Diversity,Terrestrial Mollusks, TNGMb
ISOLASI, ENUMERASI DAN DETEKSI MOLEKULER GEN ToxR PADA BAKTERI Vibrio parahaemolyticus DARI TAMBAK UDANG VANNAMAE DI REMBANG Thu'ti Alawiyyah; Anto Budiharjo; Agung Suprihadi
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The presence of opportunistic pathogen bacteria in shrimp pond water is caused by 3 factor. There is environmental factor, shrimp condition and the existence of bacteria. Vibrosis disesase in shrimp is harmfull to farmers because it makes reducing result and caused disease in humans. Bacteria often found among types of Vibrio. The toxR gene is known as the transmembrane regulator gene that control the expression of toxin gene. This study aims to determine the existence of toxR gene in Vibrio parahaemolyticus in pond water samples by PCR method. Bacterial isolation was performed using selective media CDVP Nissui. Enumeration of bacteria by TPC method to determine the number of suspected V. parahaemolyticus on CDVP. Extraction of bacterial DNA using QIAamp® DNA Mini Kit. A good qualitative result of the DNA extraction then amplified by toxR primer. Sequencing process sent to PT. Genetica Science. V. parahaemolyticus inoculated on CDVP media showed blue-green color because it can not ferment sucrose. Dry media CDVP Nissui can be used as a medium in the enumeration of Vibrio parahaemolyticus (1,6 x 102 CFU/ml in water sample) because it can be distinguished by the type of bacteria colony color that appears. The PCR protocol used was annealing at 62ºC with a cycle 30 times. Electrophoresis results showed that bacterial samples from pond water positively containing toxR gene with length 368 bp. From sequence base data of nucleotides, the sample has 99% equation with V. parahaemolyticus in the NCBI bank gene. Samples of V. parahaemolyticus bacteria in shrimp pond water in Rembang are known contain the toxR gene.Keyword : V. parahaemolyticus, toxR gene, enumeration, TCBS, CDVP
UJI AKTIVITAS KITIN DEASETILASE ISOLAT BAKTERI DARI KAWASAN GEOTERMAL DIENG Ghaida Afra Akhsani; Agung Suprihadi; Sri pujiyanto
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Chitinolytic bacteria is a bacterium, which is able to degrade chitin. This ability is obtained from the resulted chitinolytic enzyme. Chitin deacetylase (EC 3.5.1.41) is one of the chitinolytic enzymes, which be able to convert chitin into its derivatives. For this reason, chitin deacetylase has a chance to be an environmentally enzymatic converter of chitin. In addition, chitin derivatives have a wider potential in many fields. The objectives of this study were to obtain bacterial isolates from the mud of Sikidang Crater in Dieng geothermal field that producing chitin deacetylase enzyme, and to determine its activity characteristic of (optimum time production, optimum pH, and effect of 1 mM divalent metal ions) from the resulted chitin deacetylase enzyme. This research used completely randomized design. The data were analyzed using One Way ANOVA and Tukey HSD test. The results showed that KSR HA 24 isolates were able to produce chitin deasetylase with optimum enzyme activity of 0.668 U / ml at 18 hours production time. Optimum activity of chitin deacetylase occurred at pH 5 of 0.75 U / ml. Chitin deacetylase activity with 1 mM addition of divalent metal ions produce activator metal ions, including Mg2+, which increased the activity up to 154.43%, Fe2+ the activity up to 144.63%, and Cu2+ the activity up to 110.41%. Inhibitor metal ions, including Zn2+, which decreased the activity to 93.77%, and Mn2+ the activity to 86.46%.Keywords: Chitinolytic, Chitin Deacetylase, Enzyme Activity, pH, Divalent Metal Ions
KONSTRUKSI PLASMID PRHA SEBAGAI PEMBAWA GEN ARAA PENYANDI ENZIM L-ARABINOSA ISOMERASE DARI Thermotoga thermarum M Masfuroh; Anto Budiharjo; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

D-tagatosa as natural low-calorie sweeterners is likely to be a sugar substitute for diabetics type II. D-tagatosa sweetness levels is by 92% the sweetness of sucrose, but only 38% of calories of sucrose. This study aimed to obtain a recombinant plasmid construction pRHA::araA. Results subcloning was then used in biotransformation processes produce D-tagatosa. Propagation vector soure pRHA   was done with the cloning procedure in E. coli TOP’10. The process  used the vector source cut with enzyme NcoI and XhoI and producing pRHA vectore for gene insertion araA. AraA gene amplification was done using Polymerase Chain Reaction (PCR)  with  primers of AITth-For and AITth-Rev. Ligation was done using T4 ligase enzyme and transformed in E. coli TOP’10 by heat-shock methods. E. coli was grown in LB medium Agar with ampicillin concentration of 100 mg/ml. Selection was made on a liquid LB and LB Agar with ampicillin concentration range of 100-200 mg/ml. Based on the result of electrophoresis visualization of the pRHA::araA recombinant plasmid isolation were negative. Keyword:  L-arabinosa isomerase, araA, D-tagatosa, T. thermarum, E. coli
PRODUKSI INULINASE OLEH KHAMIR Pichia manshurica DUCC Y-015 PADA TEPUNG UMBI DAHLIA (Dahlia variabilis Willd.) DENGAN VARIASI KONSENTRASI MnSO4.H2O DAN WAKTU INKUBASI Berlian Abadianti; Agung Suprihadi; w wijanarka
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Inulinase (E.C. 3.2.1.7) is an enzyme which able to hydrolyze an inulin into fructose and fructooligosaccharides.  The main application of inulinase enzyme in the food industries is as the ingredient in producing High Fructose Syrup (HFS). Moreover, the other important applications of inulinase enzyme are to produce ethanol, inulooligosacarida (IOS), fruktooligosacarida (FOS), pullulan, sorbitol, etc.  Pichia manshurica DUCC Y-015 is kind of yeast that is capable in producing inulinase in medium containing inulin. Optimization of inulinase enzyme production needs to be done to increase inulinase production, the way that could be conducted is by the addition of metal ion and optimization of incubation time. The purpose of this research is to investigate the effect of adding mangan ion (MnSO4.­H2O) and incubation time. In conducting this study, the researcher applies experimentally research by using Randomized Complete Block Design (RCBD) factorial pattern. The first factor is concentration of MnSO4.H2O, with concentration level 0 mM (M0), 0,1 mM (M1), 0,5 mM (M2). The second factor is the variation of incubation time, i.e. 6 hours (I6), 12 hours (I12), and 18 hours (I18) with three times repetition. The collected data were analyzed using ANOVA 5% signification (α= 0,05) and completed by the Duncan test. The result of analysis shows that variation of MnSO4.H2O concentration and incubation time does not significantly influential on inulinase activity of Pichia manshurica DUCC Y-015.Keywords: Inulinase, Inulin, Pichia manshurica DUCC Y-015, MnSO4.H2O, Incubation time.
PERTUMBUHAN DAN PRODUKSI PIGMEN MERAH OLEH Serratia marcescens PADA BERBAGAI SUMBER KARBON Setiawan Wicaksono; Endang Kusdiyantini; Budi Raharjo
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Serratia marcescens is one of the red pigment producing bacteria which is widely used as natural dye. This bacterium was isolated from the sediment of hot springs in Gedong Songo, Bandungan, Semarang. S. marcescens has potential as a natural pigment producer. This study was conducted to measure the growth and production of pigments in NB medium containing different carbon sources. The sources of carbon used were glucose, fructose, maltose, and lactose. The method used were growth measurement based on dry weight value of cell and value of ODλ=600nm, measurement of reducing sugar, measurement of the acidity of the growth medium, and measurement of red pigment concentration. The results obtained in this study indicated that the provision of carbon sources has no significant effect on the growth of S. marcescens. The best carbon source for red pigment production is lactose with pigment concentration of 0.451 mg/L achieved at 24 hours incubation time.Keywords: Serratia marcescens, Growth, Carbon Source, Red Pigment.
PENGARUH CaCl2.2H2O DAN WAKTU INKUBASI TERHADAP PRODUKSI INULINASE OLEH Pichia manshurica DUCC Y-015 DALAM SUBSTRAT TEPUNG UMBI DAHLIA Dahniar Saraswati; W Wijanarka; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Fructose production from inulin by inulinase only need one reaction enzimatic step and produce 95% fructose. Inulin obtained from dahlia tuber and inulinase produced by Pichia manshurica DUCC Y-015. Inulinase production (E.C. 3.2.1.7.) can be influenced by metal salt suplementation, such as CaCl2.2H2O. The purpose of this research were to known the influence of CaCl2.2H2O and incubation time to inulinase production by P. manshurica DUCC Y-015 on Dahlia Tuber Substrate. The design that use in this research were Randomized Factorial Block Design ( RAFBD ). Factor I (CO, C­1, C2, C3) as the concentration of CaCl2.2H2O (0 mM, 0.25 mM, 0.50 mM, 1.00 mM) and Factor II ( T12, T18, dan T24 ) as incubation time ( 6, 12, 18 hour), the repetition were 3 times. The result analyze by ANOVA (Analysis of Variance) and continued by LSD test. The result of this research indicate that CaCl2.2H2O and incubation time were not significantly influence to inulinase production. The highest inulinase production by Pichia manshurica DUCC Y-015 indicate by C2T12 treatment which use 0.50 mM CaCl2.2H2O and 12 hour incubation time, the enzyme activity is 0.60 IU/mLKey Words : CaCl2.2H2O, Dahlia tuber,  Incubation time, Inulin, Inulinase, Pichia manshurica DUCC Y-015.

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