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Chem Info
Published by Universitas Diponegoro
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Engineering,
Chemical Engineering, Chemistry & Bioengineering Chemistry
CHEMINFO JOURNAL adalah jurnal saintifik yang diterbitkan secara periodik 3 bulanan oleh Jurusan Kimia, Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Jurusan Kimia.
Arjuna Subject : -
Articles 66 Documents
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Purifikasi L-Asparaginase Dari Bawang Bombay (Allium cepa L.) Menggunakan Kromatografi Filtrasi Gel Sephadex G-100 Setiawan, Ayu Sri Rahayu
Chem Info Journal Vol 1, No 1 (2013)
Publisher : Chem Info Journal

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Abstract

L-asparaginase from onion (Allium cepa L.) was purified. Some stage of the work including isolation of L-asparaginase obtain of crude extract was further fractionated with ammonium sulphate, dialysis, and gel filtration chromatography Sephadex G-100 to produce L-asparaginase with a higher purity. Analysis of L-asparaginase in the crude extract, the ammonium sulphate fractionation, and results of gel filtration chromatography Sephadex G-100 is done by determining the activity of the enzyme with Nessler method and determination of protein content by Lowry method so it can be determined the specific activity. The analysis obtained are a crude extract of L-asparaginase from onion (Allium cepa L.) with a specific activity of 30.502 units/mg protein. The highest specific activity of the fraction of ammonium sulphate is at F5 (80-100%) of 30.831 units/mg protein. F5 was further purified by gel filtration chromatography sephadex G-100, obtained the highest specific activity was in fraction 10 by 6508.250 units/mg protein with a purity level more than 200 times compared to the crude extract.
Isolasi, Identifikasi dan Uji Aktifitas Senyawa Alkaloid Daun Binahong (Anredera cordifolia (Tenore) Steenis) Marwoko, Muhammad Titis Budi
Chem Info Journal Vol 1, No 1 (2013)
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Abstract

Telah dilakukan penelitian tentang isolasi, identifikasi serta uji aktifitas senyawa alkaoid daun binahong (Anredera cordifolia (Tenore) Steenis). Serbuk daun binahong dimaserasi menggunakan pelarut n-heksana, kemudian etanol, diperoleh ekstrak n-heksana dan ekstrak etanol. Dari ekstrak etanol dilakukan isolasi alkaloid total. Pemisahan alkaloid dilakukan dengan KLT preparatif dan analisis dengan spektrofotometer UV-Vis, FTIR dan LC-MS. Dari analisis yang dilakukan diduga isolat alkaloid adalah senyawa betanidin (C18H16N2O8) yang bersifat tidak sitotoksik dengan LC50 sebesar 85,583 ppm.
PENGARUH METODE PEMANASAN DALAM OVEN DAN REFLUKS PADA SINTESIS ZEOLIT ZSM-5 DARI LIMBAH AMPAS TEBU ., Ngadiyono; ,S.Si, M.Si, Sriatun; ,M.Si, Dra. Taslimah
Chem Info Journal Vol 1, No 2 (2013)
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Abstract

The synthesis of ZSM-5 was done from bagasse waste ash and sodium silicate as source of silica and aluminium hydroxide as source of alumina with use heating method on oven and reflux. The synthesis was done by reacting sodium silicate and sodium aluminate at pH 11-12 until is formed gel. Gel through are taken to caried out heating on oven and reflux at 1100C for 1, 3, 5, dan 7 days. Product which obtained are washed by aquades until pH neutral, through are dried at 1100C for 10 hours.Characterization of product are carried out by Fourrier Transform InfraRed (FTIR) and X-Ray Diffraction (X-RD). Heating use reflux more effective than oven. Optimum result are obtained for heating at 5 days. Diffractogram are obtained showed that mixture of mineral such as Nacrite, Aluminum Silicate Oxide Hydrate, and ZSM-5. Spectrum FTIR showed that heating on reflux at wavenumber 609,51 cm-1 are showed double ring vibration of ZSM-5 framework.
PEMURNIAN SELULASE DARI ISOLAT KB KOMPOS TERMOFILIK DESA BAYAT KLATEN MENGGUNAKAN FRAKSINASI AMONIUM SULFAT Sinatari, Hanum Mustika
Chem Info Journal Vol 1, No 1 (2013)
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Abstract

Purification of cellulase enzymes derived from KB thermophilic composting isolates of Bayat Klaten using fractionation ammonium sulphate needs to be done so that cellulase can cuts cellulose chains more effectively. This study aims to gain cellulases from KB thermophilic composting isolates of Bayat Klaten is more pure than the crude cellulase extract. Purification of cellulases from KB isolates using fractionation ammonium sulphate with saturation 0-20% (F1), 20-40% (F2), 40-60% (F3), 60-80% (F4), and 80-100% (F5), followed by cellulase dialysis using cellophane membranes. Cellulases fractions activity was measured using Dinitrosalicylic acid (DNS) method and Lowry method. This study obtained cellulase with specific activity in the crude extract cellulase of 0.212 units/mg protein and the highest enzyme specific activity in fraction 2 (20-40% ammonium sulphate) of 0.749 U/mg protein. The specific activity of cellulase in fraction 2 increased 3,5 times from the specific activity of the crude cellulase extract.  
PEMANFAATAN KITOSAN DARI LIMBAH KULIT UDANG WINDU (Penaeus monodon) SEBAGAI BAHAN ANTIMIKROBA PADA PROSES PENYIMPANAN IKAN NILA (Oreochromis niloticus) Alifah, Kaviani Suciana; , M.Si., Khabibi; Widodo, M.Si., Didik Setiyo
Chem Info Journal Vol 1, No 2 (2013)
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Abstract

Abstract: Has done research on use of chitosan from shrimp skin (Peneaus monodon) waste as antimicrobial agents in the process of tilapia (Oreochromis niloticus) storage. The purpose of this research is to isolate chitin from the shrimp skin (Peneaus monodon) waste into chitosan, and use as an alternative to be a natural antimicrobial compounds as well as determine the optimum concentration of chitosan solution as a preservative of tilapia (Oreochromis niloticus). This research was carried out by preparation of chitosan from shrimp skin (Peneaus monodon) was begin with deproteination using 3.5% NaOH, demineralization using HCl 1N, and deacetylation using 50% NaOH, analyzing its deacetylation degree by baseline method. Then it was used as an antimicrobial of tilapia, tilapia was immersed in a solution of chitosan by varying the concentration (w/v) 1%, 1.5% and 2% in 2% acetic acid. Storage was varied within 0 days, 1 day, 2 days; 3 days, and 4 days. The results of extraction was found white-grayish colour chitin and white chitosan with deacetylation degree of 66.73%. The optimum concentration of chitosan as an antimicrobial agent of tilapia is 2% with storage time of four day which preserve the fish is consumable.
Pengaruh Ion Logam Co2+ dan Cu2+ Pada Proses Fotodegradasi Direct Blue 3R Menggunakan Fotokatalis Komposit ZnO-Karbon Aktif Wicaksono, Aji Putro
Chem Info Journal Vol 1, No 1 (2013)
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Abstract

Been conducted synthesis and characterization of ZnO-activated carbon composites, and examines the impact of the presence of metal ions Co2+ and Cu2+ in the process of photodegradation Direct Blue 3R and photoreduction metal ions. Synthesis of ZnO with the XRD results obtained ZnO (wurzite) D = 27.97 nm; Eg = 3.8 eV. Composite ZnO-activated carbon produced in tablet form with d = 0.8 cm; t = 0.45 cm gray. SEM and BET characterization of the resulting composite has a hollow morphology with a surface area ZnO-carbon composite activated by 27.209 m2/g, pore volume of 0.089 cc/g; finger pores of 3.25 nm and has a high effectiveness in the photodegradation of dye Direct Blue 3R of 95.36%. The optimum pH for photodegradation process is at pH 4 with the effectiveness of 91.92%. The addition of metal ions Co2+ and Cu2+ can increase the effectiveness of photodegradation Direct Blue 3R and can simultaneously reduce metal ions Co2+ and Cu2+ into Co(s) and Cu (s) with the optimum concentration of each metal is 12 ppm. The optimum time for photodegradation process is 2 hours. Photodegradation effectiveness of 94.875% and 76.31% on the addition photoreduction Co2+, whereas the addition of Cu2+ photodegradation effectiveness of 96.208% and 89.33% photoreduction.
FERMENTASI RUMPUT LAUT Eucheuma cottonii OLEH Lactobacillus plantarum Wandansari, Baiq Daraquthni
Chem Info Journal Vol 1, No 1 (2013)
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Abstract

Eucheuma cottonii has been known contain nutrients such as proteins, lipids, carbohydrates, vitamin C, α-tocopherol, and minerals. Nutrient content of seaweed can be utilized as a medium for the growth of lactic acid bacteria (LAB). Fermented seaweed by LAB can be used as probiotic drinks for patients with lactose intolerance. Fermentation by LAB produces primary metabolites like lactic acid, CO2, acetaldehyde and hydrogen peroxide. This study aims to obtain characteristic data of fermented E. cottonii of West Lombok, NTB by Lactobacillus plantarum (texture, color, smell, taste and pH). Fermentation E. cottonii by L. plantarum performed by addition of 5% glucose and 5% inoculum, and then incubated at 37 ° C for 72 hours. The results showed that the fermentation of E. cottonii by L. plantarum produce fermented seaweed with characteristics: yellow-green jelly, yogurt sour odor, has a sour taste and slightly bitter with a pH of 3.
PEMANFAATAN LIMBAH KULIT UDANG WINDU (Penaeus monodon) SEBAGAI EDIBLE COATING DAN PENGARUHNYA TERHADAP KADAR ION LOGAM Pb(II) PADA BUAH STROBERI (Fragaria x ananassa) Marzuki, Qosim
Chem Info Journal Vol 1, No 1 (2013)
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Abstract

A study on the utilization of leather wastes tiger shrimp (Penaeus monodon) as an edible coating and effect of Pb (II) in strawberries (Fragaria x ananassa). This research aims to synthesize chitosan from chitin of the shrimp shell waste, to determine the optimum concentration of chitosan as edible coating and determine the effect of chitosan to adsorption Pb (II) on the surface of strawberries. Experiment were carried out in several stages of research starting with the isolation of chitin by demineralitation and deproteination and than transforming it into the chitosan by deacetylation. The result is then analyced by of FTIR. Further manufacture of edible coating, the coating of fruit, and analysis of fruit quality after coating, including testing of acidity (pH) strawberries, Vitamin C, and test Pb metal adsorption on the surface of the fruit, and organoleptic test was done visual observation of physical appearance of strawberries. The results obtained chitosan can be synthesized from shrimp shell waste with 70.03% degree of deacetylation. Chitosan 2% as the coating was able to maintain the qulity of strawberries fruit longer than without coating until the fifth day. Chitosan as coating agent was also able to reduce the presence of metal ions Pb2+ on the strawberries surface
PENGARUH PENAMBAHAN KITOSAN TERHADAP KARAKTER SEMEN KALSIUM FOSFAT Kusumawati, Fitria
Chem Info Journal Volume 3, Nomor 1, Tahun 2015
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Abstract

Telah dilakukan penelitian tentang pengaruh penambahan kitosan terhadap karakter semen kalsium fosfat. Tujuan dari penelitian ini adalah mengetahui pengaruh penambahan kitosan terahadap setting time, hardening time, struktur kalsium fosfat, kristalinitas dan ukuran bulir kristal pada semen kalsium fosfat. Metode yang digunakan untuk membuat fase powder adalah metode reaksi padatan temperatur tinggi dan untuk membuat semen kalsium fosfat adalah metode reaksi pelarutan. Semen yang dihasilkan selanjutnya dikarakterisasi menggunakan spektroskopi Fourrier Transform Infra Red (FTIR) dan XRay Diffraction (XRD). Hasil penelitian menunjukkan bahwa pengaruh penambahan kitosan terhadap semen kalsium fosfat adalah adanya gugus fungsi fosfat, karbonil dan hidroksil yang muncul bersama gugus fungsi N-H, C-H, dan C-O pada semen kalsium fosfat-kitosan, meningkatkan setting time dan hardening time, tidak menyebabkan perubahan struktur, menurunkan kristalinitas dan ukuran bulir kristal hidroksiapatit dari 58,14 nm menjadi 51,70 nm, eningkatkan kristalinitas dan ukuran bulir kristal α-trikalsium fosfat dari 36,47 nm menjadi 41,96 nm.
AKTIVITAS Fusarium oxysporum DALAM MENGHIDROLISIS ECENG GONDOK (Eichhornia crassipes) DENGAN VARIASI WAKTU FERMENTASI Tristianti, Siska Yulyana
Chem Info Journal Vol 1, No 1 (2013)
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Abstract

Fusarium oxysporum activity to hydrolyze water hyacinth (Eichhornia crassipes) with variations of fermentation time taken to get the Fusarium oxysporum that have adapted to the fermentation medium of water hyacinth (Eichhornia crassipes) and obtain activity data of Fusarium oxysporum in hydrolyzing water hyacinth (Eichhornia crassipes) on variations of fermentation time. Activities Fusarium oxysporum based on the ability to hydrolyze water hyacinth mass accretion based cell (dry weight) produced. This research found that Fusarium oxysporum capable of growing on water hyacinth fermentation media and the activity of Fusarium oxysporum to hydrolyze water hyacinth occurred at optimum fermentation time at hours-168 which is the exponential phase of Fusarium oxysporum.

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