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Nusantara Bioscience
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 16 Documents
Search results for , issue "vol. 14 no. 2 (2022)" : 16 Documents clear
Short Communication: Antioxidant activity of ethanol extract of Chlorella sorokiniana cultured in tofu wastewater HAMZA MURSANDI; DEVY SUSANTY; LANY NURHAYATI; ADE AYU OKSARI
Nusantara Bioscience Vol. 14 No. 2 (2022)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n140204

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Abstract. Mursandi H, Susanty D, Nurhayati L, Okasari AA. 2022. Short Communication: Antioxidant activity of ethanol extract of Chlorella sorokiniana cultured in tofu wastewater. Nusantara Bioscience 14: 155-159. Microalgae are microorganisms that grow quickly and produce secondary metabolites with antioxidant activity. Antioxidants of microalgae can be utilized in various aspects such as cosmetics, pharmaceuticals, supplements, and feed. Microalgae utilization will be more profitable if the microalgae can be cultured on waste media. This study aims to determine the concentration of a suitable medium for the growth of Chlorella sorokiniana Shihira & R.W.Krauss, total flavonoids, total phenolics, and the potential of ethanolic extract of C. sorokiniana as an antioxidant. This study cultured the microalgae C. sorokiniana on tofu liquid waste media at various concentrations (15, 20, 25, and 30%). The growth of C. sorokiniana on the media was observed using a spectrophotometer at 680 nm wavelength. C. sorokiniana biomass was collected on the 7th day. The biomass was extracted using ethanol as a solvent. Phytochemical analysis was performed using the standard method, Total phenolic content, total flavonoid content, and antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) were conducted to determine the IC50 value. The results showed that the best growth of C. sorokiniana was on TLW media at a concentration of 30%. The ethanolic extract of C. sorokiniana showed the presence of alkaloids, flavonoids, steroids, tannins, and saponins. The total phenolic content in the ethanolic extract of C. sorokiniana was 18.39 ± 0.29 mgGAE/g, and the total flavonoid content was 31.93 ± 5,60 mgQE/g. The IC50 of the ethanolic extract of C. sorokiniana was 288.95 mg/L, which shows this extract has a potent antioxidant.
Association of thyroid disorders with diabetes: A cross-sectional study MUBASHIR ZAFAR; SYED MONWAR ALAM SHAHID; REEM FALAH ALSHAMMARI; MOHD ADNAN KAUSAR; TARIG A. N. GINAWI; ABDUL WAHAB HATIM; ABDUL MAJEED WADI; HAMAD ALI; ABDUL AZIZ HAMED; MOATH SALEH AL-ZAHRANI; ALI HUSSAIN; ABDULAZIZ SAUD ALDUHAIM; ABDULMAJEED MOHAMMED
Nusantara Bioscience Vol. 14 No. 2 (2022)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n140201

Abstract

Abstract. Zafar M, Shahid SMA, Alshammari RF, Kausar MA, Ginawi TAN, Hatim AW, Wadi AM, Ali H, Hamed AA, Al-Zahrani MS, Hussain A, Alduhaim AS, Mohammed A. 2022. Association of thyroid disorders with diabetes: A cross-sectional study. Nusantara Bioscience 14: 135-140. Two common endocrine disorders that correlate with each other are diabetes mellitus (DM) and thyroid dysfunction (TD). Undiagnosed thyroid disorders (TD) have a high risk for diabetes mellitus (DM) patients. A common complication among these patients is cardiovascular disease. This study aims to assess the association of TD among diabetes patients. It is a cross-sectional study, and 338 patients with type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM) were observed through a simple random sampling method from a public sector hospital. The diabetes status of patients was confirmed through clinical and laboratory investigation. Those patients who were under treatment of thyroid were excluded from the study. The chi-square test was used for analysis, and a p-value <0.05 was considered significant. The frequency of TD among diabetic patients was 47.6%. The main type of TD was subclinical hypothyroidism, and its prevalence is 43.8% and 23.5% among patients with T1DM and T2DM, respectively. Subclinical hyperthyroidism prevalences are 12.3% and 24.4% among TIDM and T2DM patients, respectively. The study found a high-frequency rate of TD among DM patients. Therefore, there is a need for regular screening of DM patients for TD and increased awareness regarding TD among DM patients.
Effect of foliar zinc application on growth and yield of rice (Oryza sativa) in the Indo-Gangetic Plains of India RAHUL SAIKH; KANU MURMU; ARUP SARKAR; RAMYAJIT MONDAL; KALYAN JANA
Nusantara Bioscience Vol. 14 No. 2 (2022)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n140208

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Abstract. Saikh R, Murmu K, Sarkar A, Mondal R, Jana K. 2022. Effect of foliar zinc application on growth and yield of rice (Oryza sativa) in the Indo-Gangetic Plains of India. Nusantara Bioscience 14: 182-187. A field experiment was conducted on rice cv. Satabdi (IET-4768) to investigate the effect of foliar zinc application at different stages during the post-Kharif season of 2019. The field experiment was carried out at 'C' block farm of (B.C.K.V), Kalyani, India, with eight different foliar 0.5% 0.5% ZnSO4 (ZnSO4) are T1: Control (without foliar application), T2: Foliar application of 0.5% ZnSO4 at Panicle Initiation, T3: Foliar application of 0.5% ZnSO4 at Booting, T4: Foliar application of 0.5% ZnSO4 at Panicle Initiation and 1 week after flowering, T5: Foliar application of 0.5% ZnSO4 at Panicle at 1 week after Flowering, T6: Foliar application of 0.5% ZnSO4 at Panicle at 2 weeks after flowering, T7: Foliar application of 0.5% ZnSO4 at Panicle at 1 week and 2 weeks after flowering and T8: Foliar application of 0.5% ZnSO4 at Panicle Initiation, Booting, 1 week and 2 weeks after flowering respectively in randomized complete block design with three replication. The result of the experiment revealed that rice plants treated with the combination of T4i.e. Foliar application of 0.5% ZnSO4 at Panicle Initiation and 1 week after flowering have resulted in the highest grain yield of 5.09 t/ha, which was 50.59% higher (3.38 t/ha) than the T1i.e. the control. Furthermore, residual nutrient status was also highest in the plot treated with T4i.e. Foliar application of 0.5% ZnSO4 at Panicle Initiation and 1 week after flowering.
The addition of vermicompost and biostarter affects the growth, total phenolic and antioxidant activity of Echinacea purpurea LUTFIA FAJAR CHOIRUNNISA; YULI WIDIYASTUTI; SOLICHATUN SOLICHATUN; AHMAD YUNUS
Nusantara Bioscience Vol. 14 No. 2 (2022)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n140203

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Abstract. Choirunnisa LF, Widiayastuti Y, Solichatun, Yunus A. 2022. The addition of vermicompost and biostarter affects the growth, total phenolic and antioxidant activity of Echinacea purpurea. Nusantara Bioscience 14: 148-154. Echinacea purpurea (L.) Moench or purple coneflower is a medical plant that originated in North America and contained various bioactive compounds, one of which is phenolic. Applying organic fertilizer like vermicompost has been reported to increase plants' bioactive compounds' components and antioxidant activity. The purpose of this study was to determine the addition of vermicompost and biostarter on the growth, total phenolic and antioxidant activity of E. purpurea. Split-Plot Randomized Complete Block Design was used with dosages of vermicompost 0, 40, 60, and 80 g/plant and different types of biostarter from Banana peel waste and effective microorganisms (EM). The results showed that treatment of 80 g/plant vermicomposts and EM highest resulted in the growth rate parameters (plant high, leaf numbers, leaf area, roots volume, plant fresh and dry weight) and total phenolic content with 1.802%. On the other hand, the herb extracts had the highest result with the treatment of 40 g/plant vermicomposts and EM (4.96%). The antioxidant activity was tested using the DPPH method with TLC and showed that all treatments indicated positive antioxidant activity.
Acute toxicity, biochemical and histological of fenitrothion and thiobencarb on fish Nile tilapia (Oreochromis niloticus) Mohamed Riad Fouad; Ahmed Farahat El-Aswad; Maher Ibrahim Aly
Nusantara Bioscience Vol. 14 No. 2 (2022)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n140213

Abstract

Abstract. Fouad MR, El-Aswad AF, Aly MI. 2022. Acute toxicity, biochemical and histological of fenitrothion and thiobencarb on fish Nile tilapia (Oreochromis niloticus). Nusantara Bioscience 14: 217-226. The results show that the tested fenitrothion and thiobencarb are highly toxic to fish. However, fenitrothion is more toxic (1.6 times) on Oreochromis niloticus (Linnaeus, 1758) than thiobencarb. The determined 96-h LC50 values using a static bioassay system to Nile tilapia fingerlings (8-10 g) were 0.20 and 0.32 mg L-1 for fenitrothion and thiobencarb, respectively. The mortality rate of fish exposed to ½ 96-h LC50 of fenitrothion (0.10 mg/L) and thiobencarb (0.16 mg/L) for four days demonstrated was 20% mortality rate. Fish showed tremors, lethargy, decreased movement, and increased respiratory rhythm. The total activity of AChE in control was 5.61 ±0.03; it was significantly reduced to 4.92 ±0.03 in fenitrothion treatment and 1.13 ±0.02 in thiobencarb treatment. Fenitrothion decreased the specific activity from 0.83 ±0.01 for the control to 0.68 ±0.01, whereas thiobencarb reduced the specific activity to 0.22 ±0.01. Generally, thiobencarb inhibited AChE activity much more than fenitrothion; it produced 80% inhibition, while fenitrothion produced 12.5% inhibition. It showed a significant increase in liver GST and SOD activity of Nile tilapia exposed to the tested pesticides compared to the control. There were no histological alterations in the tissues of the control individuals. It was found that the herbicide thiobencarb affected the gills, kidneys, and liver of Nile tilapia more than the insecticide fenitrothion.
Effect of storage temperature and packing materials on seed germination and seed storage behavior of Schefflera abyssinica TURA BAREKE; ADMASSU ADDI; KASIM ROBA; TOLERA KUMSA
Nusantara Bioscience Vol. 14 No. 2 (2022)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n140202

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Abstract. Bareke T, Addi A, Roba K, Kumsa T. 2022. Effect of storage temperature and packing materials on seed germination and seed storage behavior of Schefflera abyssinica. Nusantara Bioscience 14: 141-147. Knowledge of seed storage behavior is crucial for developing appropriate ex-situ conservation strategies. The main objective of this study was to determine the seed storage behavior of Schefflera abyssinica (Hochst. ex A. Rich.). A factorial combination of three temperatures (-10, 0, and 22°C) levels, two types of packing containers (polythene bag and aluminum bag), and eight periods of storage (0, 30, 60, 90, 120, 150, 180 days, and for a year) level was used to determine the germination capacity and storage behavior of the seeds. Accordingly, seed storage temperatures and storage period (up to 1 year) have a significant effect on the germination (p<0.01) of S. abyssinica. The highest average germination percentage of S. abyssinica seeds was obtained after 2 months of storage under all conditions. The seed storage period influences the germination of S. abyssinica by 47.8%. The germination percentages of S. abyssinica seeds have shown significant differences in storage temperatures. Seeds stored at -10°C showed the highest germination percentage in all storage periods compared to the two storage temperatures. Generally, the highest germination capacity of S. abyssinica seeds was between 7% and 9% moisture content after 60 and 90 days of storage. Packing materials have no significant difference in the survival of seeds. No stored seeds germinated after one year of storage at room temperature, 0°C, and -10°C. Based on the definition of seed storage behavior, we conclude that seeds of S. abyssinica have intermediate storage.
Somatic embryogenesis of the selected intergeneric hybrid between Phalaenopsis 2166 and Vanda ‘Saint Valentine’: Application of NAA and TDZ Murni Dwiati; AGUS HERY SUSANTO; Iman Budisantoso
Nusantara Bioscience Vol. 14 No. 2 (2022)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n140205

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Abstract. Dwiati M, Susanto AH, Budisantoso I. 2022. Somatic embryogenesis of the selected intergeneric hybrid between Phalaenopsis 2166 and Vanda' Saint Valentine': Application of NAA and TDZ. Nusantara Bioscience 14: 160-165. Intergeneric hybridization between Phalaenopsis 2166 and Vanda' Saint Valentine' has produced a hybrid seedling with several characters potentially developing into plant individuals with flowers of better performance. Therefore, identical clones of the selected hybrid should be developed into PLBs using an in-vitro culture technique employing somatic embryogenesis supported by the application of plant growth regulators. This study aims to unveil the effect of NAA and TDZ in stimulating the formation of identical clones of the selected intergeneric hybrid between Phalaenopsis 2166 and Vanda' Saint Valentine'. The experiment was arranged in a factorial Randomized Complete Block Design (RCBD) involving two factors, i.e., types of plant growth regulators and the levels of concentrations of each substance. It was found that the combination of NAA and TDZ significantly affected the growth of the identical clones. Furthermore, the combination of NAA 0.5 mgL-1 and TDZ 1.5 mgL-1 resulted in clones that potentially differentiate into PLBs. This finding indicates that NAA and TDZ should be applied appropriately to stimulate somatic embryogenesis in the intergeneric hybrid.
Diversity studies on insect pests of high altitudinal transitional zones of North-western Himalayas PAWAN KUMAR; TAMANNA SINGH THAKUR; DEEPIKA DEEPIKA; NEHA SHARMA
Nusantara Bioscience Vol. 14 No. 2 (2022)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n140211

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Abstract. Kumar P, Thakur TS, Deepika, Sharma N. 2022. Diversity studies on insect pests of high altitudinal transitional zones of North-western Himalayas. Nusantara Bioscience 14: 203-210. Class Insecta constitute a major fauna and comprise many species of economic importance. Due to climate change and increase in temperature, many insect species are changing their habitat considerably and shifting their hosts, which leads to changes in the diversity of insect pests at different altitudinal gradients. High altitude forest cover is not large and massive as plains forest cover, but it supports some of the very important economical tree species like- Quercus sp., Himalayan Poplar, Betula sp., Abies pindrow (Royle ex D.Don) Royle, Juniper spp., Birdcherry, Maple, etc. The present study analyzed any change in insect pest incidences and diversity of pest species due to the change in host preferences or climatic patterns. The study was conducted at four selected sites viz. Rohtang area (Kullu Forest Division), Chanshal area (Rohru Forest Division), Sach area (Churah Forest Division), and Indrahar area (Dharamshala Forest Division) of high altitudinal transitional zones of Himachal Pradesh, India, to study insect pest diversity. A total of 32 insect species were recorded during the study period comprised of the insect orders viz., Coleoptera, Hymenoptera, Hemiptera, Orthoptera, Dermaptera, and Lepidoptera. The present investigation led to the finding that species of Coleoptera (beetles) were the most dominant insects attacking trees of the high altitudinal transitional zone, followed by Lepidoptera (Butterflies and moths) and Hemiptera (aphids).
Fruit development and capsaicin content of hot pepper (Capsicum annuum) plant cultivated in different soil salinity stress PURIN CANDRA PURNAMA; ISSIREP SUMARDI; L. HARTANTO NUGROHO
Nusantara Bioscience Vol. 14 No. 2 (2022)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n140206

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Abstract. Purnama PC, Sumardi I, Nugroho LH. 2022. Fruit development and capsaicin content of hot pepper (Capsicum annuum) plant cultivated in different soil salinity stress. Nusantara Bioscience 14: 166-171. Land scarcity for cropping at Java Island is a challenge for scientists to look for alternative cropping land. The use of saline land for cropping needs to have further discussed. Red pepper (Capsicum annuum L.) can be used as a plant model because, aside from being used as a vegetable, it is also used as natural medicine because of its secondary metabolite, capsaicin. A harsh environment could induce changes in the primary metabolism, which leads to secondary metabolite decomposition. For example, plants respond to stress, such as salt stress, by synthesizing flavonoids and phenolic acid as defense systems to reduce damage. However, the total sugar level and organic acids are decreased. This research aimed to study the fruit development and capsaicin content of hot pepper grown on various coastal soil sand to know whether or not different growth medium affects the size of each part of the fruit. The design of this research was a Completely Randomized Block Design (CRBD). In this research, five different salinity mediums were used, they were A. 15.20 dS/m, B. 5.70 dS/m, C.1.10 dS/m, and D. 2.85 dS/m obtained from Pandansimo and E. 3.25 dS/m obtained from Sleman, Yogyakarta, Indonesia, as comparation. Seedlings were transferred to the polybag after having four truly expanded leaves. Fruit development was observed every week, starting from the first day after flowering (DAF) to 35 DAF. Pericarpium and placenta thickness, fruit diameter, number, length, and width of the giant cell were recorded appropriately from the slides prepared using the paraffin method. Capsaicin content was determined at 14 and 35 DAF, performed with Gas Chromatography-Mass Spectrometry (GC-MS). The results show structural changes in the exocarpium; on the first day after flowering, there was only one layer of epidermis cells, but at 7 DAF, there was one layer of epidermis cells and one layer of collenchyma cells. Next, at 14 DAF, one layer of epidermis cells and two layers of collenchyma cells are observed. The structure of the mesocarpium, endocarpium, and placenta were not changed. The capsaicin content of the green fruit (14 DAF) was lower than the mature one (35 DAF) in all survival mediums. The highest capsaicin content at 14 and 35 DAF was obtained from a plant grown at medium C. Different growing mediums affected pericarpium and placenta thickness, number, length, and width of the giant cell fruit diameter.
Diversity, floral phenology, and socio-economic importance of melliferous plants in Eastern Ethiopia AMARE FASSIL; TSEGAW HABTAMU; MUHDIN TAHIR; TEMESGEN TEREFE
Nusantara Bioscience Vol. 14 No. 2 (2022)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n140207

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Abstract. Fassil A, Habtamu T, Tahir M, Terefe T. 2022. Diversity, floral phenology, and socio-economic importance of melliferous plants in Eastern Ethiopia. Nusantara Bioscience 14: 172-181. Beekeeping is a supply of extra money and financial gain for many thousands of farmer beekeepers in Ethiopia and plays a big role in preserving natural resources. Honeybees and flowering plants have co-evolved in their special symbiotic relationship. Bee plant types and their flowering duration differ from one place to another due to variations in topography, climate, and other cultural and farming practices. This study investigated and documented the diversity and floral phenology of honeybee plants in Doba, Gemechis, and Mi’eso Districts, Oromia National Regional State, Eastern Ethiopia, from January 2019 to July 2021. Ethnobotanical data were collected to reveal the diversity of melliferous plants, practices, and communities’ attitudes about honey production and melliferous plant conservation. A total of 422 respondents participated through semi-structured interviews, focus group discussions, and field walks for socio-economic data collection. Descriptive statistics such as frequencies, ranking, and scores were used and presented with tables and figures to analyze ethnobotanical data. A total of 120 melliferous plant species were distributed under 108 genera and 55 families, of which 70 plants were found in the Gemechis District, followed by Doba and Mi’eso Districts with 47 and 42 plants each, respectively. Sorenson’s Similarity Index values showed the wide-ranging melliferous plant species distribution patterns in the three districts with 50.4 (between Doba and Mi’eso), 37.5 (between Doba and Gemechis), and 15.3 (between Gemechis and Mi’eso) species overlaps. Fabaceae and Asteraceae contribute a significant number of species, with 12 (10 %) and 9 (7.5 %) melliferous plants, respectively. Local communities have a good awareness of the seasonal availability of melliferous plants, indicating adequate supply (June to early December) and critical shortage (November to early May) of melliferous plant resources favoring strong and weak colony strength, respectively. Lack of nutrition, improper management practices, honey bee predators, and lack of beekeeping knowledge and equipment were the most important constraints deleteriously influencing the honey quality and amount in the study area. The shortage of pollen and nectar flow during the dearth periods (January to March) needs interventions like hive migration and bee floral plantations. Hence, there is an urgent need for intervention through awareness creation, campaign-based melliferous plant plantations, and technology transfers.

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