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Nusantara Bioscience
Published by Universitas Sebelas Maret
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
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Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 16 Documents
 1   2 
Search results for , issue "vol. 6 no. 1 (2014)" : 16 Documents clear
Optimization of in vitro sterilization protocol for obtaining contamination-free cultures of Tilia platyphyllos VAHIDEH PAYAMNOUR; KAMAL GHASEMI BEZDI; MOSTAFA MEHRDAD; AKRAM AHMADI
Nusantara Bioscience Vol. 6 No. 1 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060102

Abstract

Abstract. Payamnour V, Ghasemi Bezdi K, Mehrdad M, Ahmadi A. 2014. Optimization of in vitro sterilization protocol for obtaining contamination free cultures of Tilia platyphyllos. Nusantara Bioscience 6: 7-12. Tilia platyphyllos is one of threatened species of Caspian forests. Tissue culture techniques are applied for culture, regeneration and genetic resources preservation. Utilizing an accurate sterilization procedure is important to reduce the cost, time and energy. The aim of this present study was to provide optimization of in vitro sterilization protocol to obtain contamination-free cultures of T. platyphyllos. Explants were collected randomly from the best individuals of T. platyphyllos, which were located in Tooskestan forest of Gorgan, Iran. Results revealed that the optimum protocol for sterilization was when explants were exposed in pre-sterilizing solution of 600 mg L-1 ascorbic acid, 4 g L-1 captan fungicide and 5% commercial sodium hypochlorite (NaOCl) solution (5% Cl activated) for 20 minutes and then explants were exposed in sterilizing solution containing 600 mg L-1 ascorbic acid and 10% sodium hypochlorite.
Leaching and heating process as alternative to produce fish protein powder from Kilka (Clupeonella cultiventris caspia) KAVEH RAHMANIFARAH; BAHAREH SHABANPOUR; AMIR REZA SHAVIKLO; NEHRAN AALAMI
Nusantara Bioscience Vol. 6 No. 1 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060101

Abstract

Abstract. Rahmanifarah K, Shabanpour B, Shaviklo AR, Aalami M. 2014. Leaching and heating process as alternative to produce fish protein powder from Kilka (Clupeonella cultiventris caspia). Nusantara Bioscience 6: 1-6. The effect of protein extraction procedures (leached mince and heated suspension) on selected properties of fish protein powder (proximate composition, pH, color, density, viscosity, fat adsorption, emulsifying capacity, emulsifying stability, foaming capacity, foaming stability, WBC, protein solubility in water, hygroscopicity, Trichloroacetic acid (TCA)-soluble peptides and free sulfhydryl groups) was investigated. Results showed that Fish protein powder (FPP) produced by leaching mince (LM) have higher protein, moisture, ash, pH, L*, viscosity, emulsion capacity, emulsion stability, foam capacity, foam stability, water binding capacity (WBC), protein solubility, hygroscopicity, TCA soluble peptides and free sulfhydryl group content than heated suspension (HS) (P<0.05). However, HS had higher fat and density in comparison with LM (P<0.05). No significant differences between a*, b* and fat adsorption were observed (P>0.05). Overall, it was observed that high temperature during heating of suspension in HS method makes possible protein denaturation and aggregation. Consequently, based on functional, chemical and physical properties, extraction of fish protein by leaching process was found to be suitable for the production of fish protein powder.
Hydrophysical, chemical and microbial properties of imported green waste composts SAIFELDIN A.F. EL-NAGAREABI; ABDULKADIRI E. ELSHAFIE; HUDA ALBURASHDI
Nusantara Bioscience Vol. 6 No. 1 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060103

Abstract

Abstract. El-Nagerabi SAF, Elshafie AE, Alburashdi H. 2014. Hydrophysical, chemical and microbial properties of imported green waste composts. Nusantara Bioscience 6: 13-18. To study the hydrophysical, chemical and microbial properties of the imported green waste composts (GWCs) and their suitability as an alternative to agrochemicals, four types of GWCs (Florabella, Mikskaar, Potgrond, and Shamrock) were selected. All composts showed normal physical properties, except weed seeds in Shamrock. The germination indexes comparable to the standard (90%) were 100% for Mikskaar followed by Florabella (97%), Potgrond (95%), and Shamrock (92%). Variations in physicochemical properties were shown as acidic pH 5.1-6.5 (standard 5-8), electrical conductivity (EC) 0.8-1.8 mScm-1 (standard 0.0-4.0 mScm-1), moisture content (MC) 54-70.5% (standard 35-60%) and water holding capacity (WHC%) 400-800%. The chemical properties were expressed as ammonia concentrations 2871-6565 mg kg-1 (standard <500 mg kg-1), organic matter 53.3-66.2% (standard 35%). The concentrations of heavy metals (Zn, Ni, Pb, Hg, As, Cd, and Cr) were lower than the recommended levels. The bacterial colony forming unit per gram compost ranged between 330-2870 cfu/g, the most probable number (MPN) for coliform bacteria was 23-460 cfu/g, whereas the fungal cfu were 30-1800 cfu/g. Aspergillus niger was the predominant fungus recovered from all compost samples (100%), followed by A. fumigatus (75%), whereas A. sparsus, A. versicolor and yeasts (50%), and the remaining species of the genus Acremonium sp., Aspergillus flavus, A. restrictus, Cladosporium spp., and Penicillium spp. recovered from 25% of the samples. Generally, these composts revealed normal hydrophysical properties with obvious variation in moisture contents and elevated chemicals and microbial contamination. Therefore, there is an urgent need for quality control measurements and restrict abide to legislations and quarantine regulations.
The mycobiota associated with paper archives and their potential control SAIFELDIN A.F. EL-NAGAREABI; ABDULKADIRI E. ELSHAFIE; UMAIMA A AL-HINAI
Nusantara Bioscience Vol. 6 No. 1 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060104

Abstract

El-Nagerabi SAF, Elshafie AE, Al-Hinai UA. 2014. The mycobiota associated with paper archives and their potential control. Nusantara Bioscience 6: 19-25. Historical collections kept in archives and libraries represent a cultural and artistic heritage of innumerable value. Recently in Oman, more than seventy thousand documents were collected from different countries and displayed as archives showed evident sign of mold contamination. The objectives of the present study were to screen these archives for mold invasion and a test for the effective control measure. For this, 102 samples were collected from documents of different sources and incubated on potato dextrose agar (PDA) at ambient temperature (25 â—‹C±2). The isolated fungi were identified microscopically and confirmed with DNA extraction, PCR and DNA sequencing. Twenty-two fungal species belonging to 11 genera were recovered. The genus Penicillium (46.8%) was the most prevalent, followed by Aspergillus (30.7%), Cladosporium (7%), Rhizopus (4%), and Chaetomium (3.5%) whereas the remaining 6 genera represent only 8%. Eleven species were previously reported from similar substrates, whereas 11 species and one genus are new records for the mycoflora of archives. Sodium hypochlorite at 0.3-5.2% completely inhibited the fungal growth of the 10 tested fungal isolates with minimum inhibition concentration at 0.7%. Fumigation of books with 0.7-5.2% sodium hypochlorite completely inhibited all fungi without evident damage of the documents or ink discoloration. Therefore, sodium hypochlorite can be recommended as effective and eco-friendly disinfectant for archives comparable to other hazardous chemicals.
Microbial water quality of coastal recreational water in the Gaza Strip, Palestine MAZEN T. ABUALTAYEF; ABDEL FATTAH N. ABD RABOU; AHMED A. ABU FOUL; SAID M. GHABAYEN; HASSAN M. ELSINWAR
Nusantara Bioscience Vol. 6 No. 1 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060105

Abstract

Abstract. Abualtayef MT, Abd Rabou AN, Abu Foul AA, Ghabayen SM, Elsinwar HM. 2014. Microbial water quality of coastal recreational water in the Gaza Strip, Palestine. Nusantara Bioscience 6: 26-32. Wastewater disposal into the Mediterranean coast of the Gaza Strip has many negative effects, whether on the environment or on human health, thus microbiological analysis of seawater samples was carried out. The microbial analysis was confined on two types of fecal indicators (fecal coliform and fecal streptococci), in addition to a single type of bacteria (Pseudomonas). This study was conducted between the beginning of July 2012 to the mid of October 2012 over an area extended from the proposed Khan Younis fishing port to Gaza fishing port, with a length of about 23 km. The study area was divided into five zones. The samples were collected in two rounds: the first round included 75 samples that collected along the study area during the summer season. The second round included 19 samples that collected in the autumn season to compare it with their counterparts that have been collected in the summer season. Laboratory analysis showed the presence of contamination in many of these samples. The results also showed that the pollution was concentrated in and surrounding the mouths of wastewater outfalls. Depending on the microbial analyses, which have been collected in the first round, the fecal coliform appeared in 61% of the samples, while fecal streptococci appeared in all samples and pseudomonas appeared in 33% of the samples. The pollutants were widespread along the study area, which is the result mainly from wastewater discharge into the sea. A risk analysis was done for season variations using the second-moment method; in general, it was found that risk in both seasons was high especially in summer.
Six unrecorded species of Russula (Russulales) from Nagaland, India and their nutrient composition RAJESH KUMAR; ASHWANI TAPWAL; SHAILESH PANDEY; RAJA RISHI; GAURAV MISHRA; KRISHNA GIRI
Nusantara Bioscience Vol. 6 No. 1 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060106

Abstract

Abstract. Kumar R, Tapwal A, Pandey S, Raja-Rishi R, Mishra G, Giri K. 2014. Six unrecorded species of Russula (Russulales) from Nagaland, India and their nutrient composition. Nusantara Bioscience 6: 33-38. The young and matured carpophores of mushrooms were collected from the forests of Puliebzie, Zakhama, Pherma, Mankoi, Chungtia, and Tigit located in different districts of Nagaland, one of the north-eastern states of India. All the species were found associated with Pinus khasya, P. caribaea, P. patula, Cryptomeria japonica, Canarium resiniferum, Dipterocarpus macrocarpus, and Shorea assamica. The collected mushrooms were identified as Russula species on the basis of their macro and microscopic characteristics. The identified Russula species were also screened for their nutrient content. The protein and carbohydrate content was found to vary between 28.12 to 42.86, and 49.33 to 55%, respectively. These species [Russula aeruginea Lindblad; -Fr., R. alnetorum Romagnesi, R. brevipes Peck, R. fragrantissima Romagnesi, R. nobilis Velen. and R. ochroleuca (Pers.) Fr Gray.] are a new record from Nagaland state of India.
Uniconazole effect on endogenous hormones, proteins and proline contents of barley plants (Hordeum vulgare) under salinity stress (NaCl) MOHAMED A. BAKHETA; MOHAMED M. HUSSEIN
Nusantara Bioscience Vol. 6 No. 1 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060107

Abstract

Abstract. Bakheta MA, Hussein MM. 2014. Uniconazole effect on endogenous hormones, proteins and proline contents of barley plants (Hordeum vulgare) under salinity stress (NaCl). Nusantara Bioscience 6: 39-44. Pot experiments were carried out during two growth seasons 2010/2011 under greenhouse conditions of the National Research Centre, Dokki, Cairo, Egypt to investigate the response of barley plants (Hordeum vulgare L) grown under salinity stress (2500 or 5000 ppm) to spraying with solutions of uniconazole at 150 or 200 ppm. The obtained results showed that irrigation with saline solutions caused increases in the amounts of abscisic acid (ABA), crude protein, total soluble-protein, and proline contents. The results showed that spraying barley plants grown under saline solutions with uniconazole increased endogenous hormone contents of ABA, cytokinins, crude protein, total soluble protein, and proline but caused decreases in the amounts of endogenous indole acetic acid (IAA) and gibberellic acid (GA3). High protection of abscisic acid in treating plants with uniconazole and under salt stress (interaction effect) increases proline, proteins and soluble protein which has been proposed to act as compatible solutes that adjust the osmotic potential in the cytoplasm. Thus, these biochemical characters can be used as a metabolic marker in relation to salinity stress.
Effect of fungicides and bioagents on number of microorganisms in soil and yield of soybean (Glycine max) GAURAV MISHRA; NARENDRA KUMAR; KRISHNA GIRI; SHAILESH PANDEY; RAJESH KUMAR
Nusantara Bioscience Vol. 6 No. 1 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060108

Abstract

Abstract. Mishra G, Kumar N, Giri K, Pandey S, Kumar R. 2014. Effect of fungicides and bioagents on number of microorganisms in soil and yield of soybean (Glycine max). Nusantara Bioscience 6: 45-48. In field experiments, the effect of selected fungicides and bioagents on number of soil microorganisms and yield of soybean (Glycine max L. Merill) was investigated. The results showed that some of the crop protections preparations applied in the experiment (as seed dressing) increased the populations of the examined microorganisms after the harvest of crops. Maximum counts of bacteria were recorded with Thiamethoxam at 3 g kg-1 while Pseudomonas at 3 g kg-1 showed the highest population of fungi, Actinomycetes, Bradyrhizobium japonicum, PSB and Pseudomonas. The highest straw and grain yields of 3241.6 and 1439.4 kg ha-1, respectively, were recorded with Pseudomonas at 3 g kg-1.
Effect of nitrogen fertilizers on productivity of Urtica pilulifera plant HEND E. WAHBA; HEMAIA M. MOTAWE; ABBER Y. IBRAHIM
Nusantara Bioscience Vol. 6 No. 1 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060109

Abstract

Abstract. Wahba HE, Motawe HM, Ibrahim AY. 2014. Effect of nitrogen fertilizers on productivity of Urtica pilulifera plant. Nusantara Bioscience 6: 49-56. Urtica pilulifera L. have been known for a long time as a medicinal plant for treatments of many diseases, but its agricultural studies and chemical composition did not have enough researches. Two field experiments were carried out at the experimental farm of Faculty of Agriculture, Cairo University during two successive seasons 2009 and 2010, to study the effect of nitrogen dressing application (Urea, ammonium nitrate and ammonium sulfate) at different doses (0, 20, 40 and 60 N units/fed.) on growth, yield parameters and chemical composition of Urtica pilulifera plants. The results showed that the application of all nitrogen forms significantly increased the determined parameters as compared to untreated plants and the best treatment was ammonium sulfate at 60 N unit to increase quantitative and qualitative plant production.
Review: Potential production of carotenoids from Neurospora SRI PRIATNI
Nusantara Bioscience Vol. 6 No. 1 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060111

Abstract

Abstract. Priatni S. 2014. Review: Potential production of carotenoids from Neurospora. Nusantara Bioscience 6: 63-68. Carotenoids are abundant and widely distributed in plants, animals, and microorganisms. Commercial use of carotenoids competes between microorganisms and synthetic manufacture. Carotenoids production can be increased by improving the efficiency of carotenoid synthesis in microbes. Some of the cultural and environmental stimulants are positively affecting the carotenoid content of carotenogenic strains such as Neurospora. Neurospora is a fungus that exhibits the formation of spores and conidia, the part of the cell for carotenoids biosynthesis. The Indonesian traditional fermented food, red peanut cake or oncom, especially in West Java, is produced from legume residues of Neurospora sp. This fungus has been isolated and identified as Neurospora intermedia. In order to apply this pigment for food and cosmetic colorants, encapsulation techniques of carotenoids have been developed to improve its solubility and stability.

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