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Nusantara Bioscience
Published by Universitas Sebelas Maret
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
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Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 13 Documents
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Search results for , issue "vol. 6 no. 2 (2014)" : 13 Documents clear
G1957C variation in Core Promoter and Precore/Core regions of Hepatitis B virus isolated from men who have sex with men (MSM) in Surakarta, Central Java, Indonesia AFIONO AGUNG PRASETYO; RATNA SARIYATUN; ANGGA DWI PRASETYO; IBNU YUDISTIRO; RADEN ARTHESWARA SIDHAJATI
Nusantara Bioscience Vol. 6 No. 2 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060201

Abstract

Abstract. Prasetyo AA, Sariyatun R, Prasetyo AD, Yudistiro I, Sidhajati RA. 2014. G1957C variation in Core Promoter and Precore/Core regions of Hepatitis B virus isolated from men who have sex with men (MSM) in Surakarta, Central Java, Indonesia. Nusantara Bioscience 6: 107-110. Little is known about HBV isolates in Indonesian men who have sex with men (MSM) community. Thus, this study was aimed to analyze the genetic profile of HBV isolated from MSM in Indonesia, particularly in Surakarta, Central Java. A total of 205 blood samples were examined by polymerase chain reaction (PCR) targeting the partial Core Promoter (CP) and Precore/Core (preC/C) regions. Three samples were found positive by PCR. The amplicons were sequenced and subjected to molecular analysis. The genetic profile of all HBV isolated in Indonesia (n= 122) was also included for molecular analysis. All HBV isolated from the MSM were genotype B3. G1957C (C195S) was found in all HBV isolated from the MSM community and subject with sex with man history. Several disease-related mutations were observed among HBV sequences from Indonesia, including T1753C, A1762T, G1764A, A1846T, and G1896A which existed in 7.2% (9/125), 17.6% (22/125), 19.2% (24/125), 16.8% (21/125), and 24.8% (31/125) sequences, respectively; however, none were present in HBV isolated from the MSM. In conclusion, various mutations, including those related to disease, were found in Indonesian HBV isolates. G1957C variation was unique in the HBV isolated from the MSM.
Genetic regulation of allolysis in response to sub-lethal antibiotic stress in Streptococcus pneumoniae MANISHA DASH; HIRAK R. DASH; SURAJIT DAS
Nusantara Bioscience Vol. 6 No. 2 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060202

Abstract

Abstract. Dash M, Dash HR, Das S. 2014. Genetic regulation of allolysis in response to sub-lethal antibiotic stress in Streptococcus pneumoniae. Nusantara Bioscience 6: 111-117. Allolysis is the phenomenon of cell lysis induced by other cells of the same species. Gram-positive bacterium Streptococcus pneumoniae, a major human pathogen exhibits competence induced allolysis that increases the genetic recombination and enhances the virulence. During allolysis, a group of non-competent bacterial cells is lysed by another group of competent cells in the same culture. This process is regulated by com operon as well as bacteriocin. In this study, allolysis was induced in Streptococcus pneumoniae MTCC655 by sub-lethal dose of antibiotic (chloramphenicol) and the mechanism of allolysis has been deduced by amplification of lytA, lytC and cbpD genes in the bacterium. The strain was found to be resistant to a number of antibiotics including amoxicillin, cefpodoxime, erythromycin, and vancomycin. The early onset of allolysis induction from 7-9 h under normal conditions to 2-3 h by sub-lethal dose of chloramphenicol was observed.
Physiological and biochemical responses of thyme plants to some antioxidants SALWA A. ORABI; IMAN M. TALAAT; LAILA K. BALBAA
Nusantara Bioscience Vol. 6 No. 2 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060203

Abstract

Abstract. Orabi SA, Talaat IM, Balbaa LK. 2014. Physiological and biochemical responses of thyme plants to some antioxidants. Nusantara Bioscience 6: 118-125. Two pot experiments were conducted to investigate the effect of tryptophan, nicotinamide and ?-tocopherol (each at 50 and 100 mg/L) on plant growth, essential oil yield, and its main constituents. All treatments significantly promoted plant height, and increased fresh and dry mass (g/plant) of thyme (Thymus vulgaris L.). The treatment with 100 mg/L nicotinamide showed increases in total potassium mainly in the first cut. Total soluble sugars, oil percentage, and oil yield and protein recorded increments with tryptophan treatments. Treatment of Thymus plants with 100 mg/L nicotinamide observed the highest percentage of thymol (67.61%). Oxygenated compounds recorded the highest value with 50 mg/L ?-tocopherol treatment, while the maximum non-oxygenated ones resulted from the application of 100 mg/L nicotinamide. All treatments under study significantly affected the activity of oxidoreductase enzymes (POX and PPO). Nicotinamide at the concentration of 100 mg/L recorded the highest increments in APX and GR and the lowest values in oxidoreductase enzyme activities added to the lowest values of lipid peroxidation to enhance the best protection of thyme plants.
Effect of irrigation intervals on growth and chemical composition of some Curcuma spp. plants MAKARIM A. MOHAMED; HEND E. WAHBA; MOHMED E. IBRAHIM; ABD-ELGHANI A. YOUSEF
Nusantara Bioscience Vol. 6 No. 2 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060206

Abstract

Abstract. Mohamed MA, Wahba HE, Ibrahim ME, Yousef AA. 2014. Effect of irrigation intervals on growth and chemical composition of some Curcuma spp. plants. Nusantara Bioscience 6: 140-145. The Influence of irrigation intervals on the growth, yield of rhizomes and chemical composition of both Curcuma aromatica and Curcuma domestica plants was investigated. Three irrigation treatments were used in this experiment. The first treatment was irrigated every one week. The second and third treatments were irrigated every two and three weeks. The long irrigation intervals significantly reduced growth parameters and chemical composition. Growth parameters, i.e. plant height, number of leaves, width of the leaf, fresh and dry weight of rhizomes, as well as chemical composition, i.e. total carbohydrate, volatile oil and curcumin in dry rhizomes increased when the plants irrigated every week compared to irrigation treatments every two or three weeks. Also, C. aromatica gave the higher values of growth parameter and chemical composition compared to C. domestica under all irrigation treatments.
Biodeterioration of stored castor (Ricinus communis) seeds by Aspergillus tamarii ANTHONY NEGEDU; AMEH B. JOSEPH; VERONICA J. UMOH; SUNDAY E. ATAWODI; MAHENDRA RAI
Nusantara Bioscience Vol. 6 No. 2 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060204

Abstract

Abstract. Negedu A, Joseph Ab, Umoh VJ, Atawodi SE, Rai MK. 2014. Biodeterioration of stored castor (Ricinus communis) seeds by Aspergillus tamarii. Nusantara Bioscience 6: 126-131. Biodeterioration changes induced by Aspergillus tamarii in seeds of castor stored for a period of 180 days were investigated. Using Sabouroud Dextrose Agar (SDA) and direct plating methods, mycoflora associated with stored castor seeds were examined. The lipolytic ability of the isolates was determined based on free fatty acid production (quantitative). The effect of Aspergillus tamarii on moisture, total fat, crude protein, nitrogen-free extract, ash contents as well as free fatty acid and peroxide values were monitored bimonthly for six months during storage in the laboratory. In storage, Aspergillus tamarii caused significant (P ? 0.05) increases in free fatty acids, peroxide value, moisture content, crude protein, ash, and crude fiber contents and significantly (P ? 0.05) reduced the total fat and soluble sugar content (NFE) of the inoculated seeds compared to the uninoculated control. Aspergillus tamarii associated with stored castor seeds caused deteriorative changes in the seeds during storage period.
Endophytic Phoma sp. isolated from medicinal plants promote the growth of Zea mays ASHWINI KEDAR; DNYANESHWAR RATHOD; ALKA YADAV; GAURAVI AGARKAR; MAHENDRA RAI
Nusantara Bioscience Vol. 6 No. 2 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060205

Abstract

Kedar A, Rathod D, Yadav A, Agarkar G, Rai M. 2014. Endophytic Phoma sp. isolated from medicinal plants promote the growth of Zea mays. Nusantara Bioscience 6: 132-139. Fungal endophytes are reported as rich sources of valuable secondary metabolites and could be used as bio-fertilizers. In the present study, we report growth promotion potential of two Phoma species isolated from Tinospora cordifolia and Calotropis procera on maize. The fungal endophytes enhanced growth in inoculated maize plants compared to non-inoculated plants. The main aim of this work was to assess the growth promotion activity of endophytic Phoma species on maize isolated from T. cordifolia and C. procera.
The effects of saline irrigation water and cobalt on growth and chemical composition in Nigella sativa KHALID A. KHALID; MAHMOUD R. SHEDEED
Nusantara Bioscience Vol. 6 No. 2 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060207

Abstract

Abstract. Khalid KA, Shedeed MR. 2014. The effects of saline irrigation water and cobalt on growth and chemical composition in Nigella sativa.Nusantara Bioscience 6: 146-151. Increasing plant salinity tolerance is a focus of research and industry since salinity and yield are of major concern to maximize medicinal and aromatic plant production in arid and semi-arid areas. Therefore, the present study aimed to decrease the harmful effect of salinity on Nigella sativa L plants by adapting them to saline soil stress through the use of Cobalt. The effects of saline irrigation water and cobalt on the vegetative growth characters [plant height (cm), leaf number (plant-1), branch number (plant-1), capsule number (plant1), herb dry weight (plant-1) and seed yield (plant-1)] and content of fixed oil, soluble sugars, proline, N,P,K and protein of black seed (Nigella sativa L.) plants were investigated. In these experiments, two factors were considered: saline irrigation water and Cobalt. The experimental design followed a complete random block design. The averages of data were statistically analyzed using 2-way analysis of variance (ANOVA-2) and the values of least significant difference (LSD) at 5%. Saline irrigation water decreased certain growth characters, fixed oil, protein and mineral content (N, P, and K) as saline irrigation water level increased. Saline irrigation water promoted the accumulation of soluble sugars and proline contents. The plants treated with saline irrigation water containing cobalt resulted in higher plant growth characters and chemical constituent’s values than those treated with saline irrigation water alone.
Qualitative and financial evaluation of public and private forest nurseries; Case study of southern Zagros forests, Iran MARYAM FAZLOLLAHI MOHMMADI; SEYED MAHDI HESHMATOL VAEZIN; VAHID ETEMAD; ASHGAR SEPAHVAND; ANOSHIRVAN SHIRVANI; SOLEIMANI AZAM
Nusantara Bioscience Vol. 6 No. 2 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060208

Abstract

Abstract. Mohammadi MF, Vaezin SMH, Etemad V, Sepahvand A, Shirvani A, Azam S. 2014. Qualitative and financial evaluation of public and private forest nurseries; Case study of southern Zagros forests, Iran. Nusantara Bioscience 6: 152-158. This study has examined the efficiency of two nurseries, private and public divisions, based on qualitative and financial evaluation in the Southern Zagros forests of Iran. To achieve this purpose, we selected two species including pistachio (Pistacia atlantica) and poplar (Populus nigra) in one year period. Results of qualitative evaluation showed that all produced seedlings by private nursery significantly had better features than the public one at a significant level of 0.05. Also, the results of financial evaluation showed that cost price of each poplar seedling at private nursery (0.734 $) was 19.6 times less than public one (14.4 $). The interest rate for pistachio seedling in private sector ranges from 1.9 to 2.2 $, while it ranges from 1.5 to 1.9 $ for poplar seedling. Such researches may help to improve management as well as financial and qualitative efficiency, especially in public nurseries. Future researches will be required to document other aspects of private management on nurseries in seedling products.
Mushroom refinement endeavor auspicate non green revolution in the offing SHAUKET AHMED PALA; ABDUL HAMID WANI; ROUF HAMZA BODA; BILAL AHMAD WANI
Nusantara Bioscience Vol. 6 No. 2 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060210

Abstract

Abstract. Pala SA, Wani AH, Boda RH, Wani BA. 2014. Mushroom refinement endeavor auspicate non green revolution in the offing. Nusantara Bioscience 6: 173-185. Mushroom can serve as food, tonic, and as medicine thus make people healthier, fitter and happier. They have a cracking potential for generating great socioeconomic impact in human welfare at local, national and international level. With the help of allied mushroom farming we can easily tackle the problem of food for growing world population; reduce environmental pollution by bioconversion of huge organic wastes into mushrooms; recycle huge quantity of organic wastes to mushroom crops, biofertilizers, and biogas; restore damaged environment by mushroom mycelia through mycoforestry, mycoremediation, mycofiltration and mycopesticides in a zero-emission fashion. They can be used to degrade radioactive industrial biocide wastes in an eco-friendly fashion. Since mushroom cultivation is an indoor agribusiness, it could have a great economic impact by generating employment, income and functional food requirements for rural people, especially in developing countries. How far mushroom cultivation can meet the functional food requirements; address the domestic food challenges, rising food prices and crisis vis a vis environmental sustainability will be thrust areas of this communication.
Review: Advancements and application of immunosensors in the analysis of food contaminants MD. SHOFIUL AZAM; MD. RAMIM TANVER RAHMAN; ZAIXIANG LOU; YUXIA TANG; SALEH MUHAMMED RAQIB; JAKIA SULTANA JOTHI
Nusantara Bioscience Vol. 6 No. 2 (2014)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n060211

Abstract

Azam MS, Rahman MRT, Lou Z, Tang Y, Raqib SM, Jothi JS. 2014. Advancements and application of immunosensors in the analysis of food contaminants. Nusantara Bioscience 6: 186-195. Immunosensors are affinity ligand-based biosensor solid-state devices in which the immunochemical reaction is coupled to a transducer. The fundamental basis of all immunosensors is the specificity of the molecular recognition of antigens by antibodies to form a stable complex. This is similar to the immunoassay methodology. Immunosensors can be categorized based on the detection principle applied. The main developments are electrochemical, optical, and microgravimetric immunosensors. In contrast to immunoassay, modern transducer technology enables the label-free detection and quantification of the immune complex. The analysis of trace substances in environmental science, pharmaceutical and food industries is a challenge since many of these applications demand a continuous monitoring mode. The use of immunosensors in these applications is most appropriate. Food chemists should take advantage of immunosensors in food and clinical diagnostics. There are many recent developments in the immunosensor field which have potential impacts. The future role of this technique in intra-laboratory, as well as bedside testing, will become even more important as the food laboratory is faced with increasing pressure to contain costs. Objective of this paper is to give a general overview of the possible application of immunosensors to the food analysis field.

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