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Nusantara Bioscience
Published by Universitas Sebelas Maret
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
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Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 15 Documents
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Search results for , issue "vol. 9 no. 3 (2017)" : 15 Documents clear
Characterization and clustering of agronomic characters of several soybean genotypes M. MUCHLISH ADIE; AYDA KRISNAWATI
Nusantara Bioscience Vol. 9 No. 3 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090301

Abstract

Adie MM, Krisnawati A. 2016. Characterization and clustering of agronomic characters of several soybean genotypes. Nusantara Bioscience 9: 237-242. The development of improved soybean variety in Indonesia is mostly derived from crossing (51.19%), which requires the availability of gene source to serve as the parental stock. Characterization of 150 soybean genotypes was conducted in Probolinggo, Indonesia, from February to May 2016. The research was arranged in a randomized block design with two replicates. Clustering of agronomic characters was carried out using cluster analysis. The grouping was based on plant age characters (vegetative phase duration, generative phase duration, generative and vegetative ratio, days to maturity), growth characters (plant height, number of filled pods, number of empty pods, number of branches, number of nodes), and seed characters (100-seed weight and seed yield plant-1), which resulted in 10 groups/clusters. Cluster I, II and III consisted of 47, 80, and 13 genotypes, respectively; whereas other clusters consisted of one up three genotypes, respectively. Each cluster represents specific characters. Cluster IV that consisted of three genotypes showed a late maturity (83.5-88.5 days), high number of pods (81.8-83.3 pods plant-1) and high yield plant-1 (21.76- 25.78 g plant-1), but have small seed size (12.24-13.75 g 100 seed-1). Cluster VI consisted of one genotype, and characterized by large seed size (16.79 g 100 seed-1) and high yield plant-1 (15.76 g). Cluster IX (1 genotype) was characterized by early maturity (73 days), unbranched, but produced relatively low yield. The preference of soybean consumer in Indonesia is high yield, in addition to early maturity (< 80 days), and large seed size (>14 g 100 seed-1). Soybean genotypes within cluster IV, VI, and IX are valuable as gene source in the development of superior soybean varieties in Indonesia.
The effect of growing season on growth rate, pod partitioning, phenology and yield variations of mungbean varieties HERDINA PRATIWI; AGUSTINA ASRI RAHMIANNA
Nusantara Bioscience Vol. 9 No. 3 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090302

Abstract

Pratiwi H, Rahmianna AA. 2016. The effect of growing season on growth rate, pod partitioning, phenology and yield variations of mungbean varieties. Nusantara Bioscience 9: 243-250. The primary objective of mungbean improvement program is the production of high yielding varieties, which is influenced by physiological trait of variety or genotype and environment. The present research aimed to study the effect of growing season on growth rate, pods partitioning, phenology and yield variations of several mungbean varieties. Field experiments were conducted at Muneng Experimental Station of the Indonesian Legume and Tuber Crops Research Institute (ILETRI), Probolinggo District, East Java, Indonesia during rainy season (October to December 2011), and dry season (May to July 2012). A randomized complete block design was employed in the present study consisted of mungbean varieties as treatments in each growing season, and three replicates. The treatments were superior mungbean varieties, i.e. Vima 1, Sriti, Murai, Kutilang and Fore Belu. The observed variables were plant phenology, plant growth rate, seed yield and yield attributes. The results indicated the growing season affected plant growth rate, pod partitioning, phenology and seed yield and yield components with rainy season influenced more parameters of plant growth rate, crop phenology, seed yield and its components as compared to dry season. Conversely, dry season had more effect on pod partitioning coefficient, total dry biomass rate, and seed size. There was significant interaction effect of genotype by growing season on total dry matter and pod growth rates, days to flowering, days to pod maturity, plant height, seed yield, plant population, pod weight, and 1000-seed weight. Seed yield varied among seasons and varieties with the average seed yield in rainy season was 75 to 119% higher than those in dry season except Fore Belu that had the same yield in both seasons.
Assessment of the successfulness of mangrove plantation program through the use of open source software and freely available satellite ILYAS NURSAMSI; WULAN RATNA KOMALA
Nusantara Bioscience Vol. 9 No. 3 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090303

Abstract

Nursamsi I, Komala WR.2017. Assessment of the successfulness of mangrove plantation program through the use of open source software and freely available satellite images. Nusantara Bioscience 9: 251-259. Mangrove forest has a major role in the process of human-environment interaction, but almost every mangrove forest in the world is under threat. In Indonesia alone, 25% of South East Asia’s mangroves are at a risk. The continued decline in mangrove forest induced by anthropogenic activity has made all the stakeholders who have the concern at the mangrove forest preservation worried, including the government. There were several programs have been performed by the government to preserve the mangrove forest. One of the programs was “Mangrove Rehabilitation Program in three districts: Ciamis, Indramayu, and Subang” held by Forestry Department of West Java Province in 2007. The aims of this study were to assess the changes in mangrove forest area before the program performed and to evaluate the successfulness of the program, using the increasing of mangrove forest area as a parameter. This study was conducted only in Subang and Indramayu Districts of West Java, Indonesia. The assessment was conducted using Landsat 4-5 TM, Landsat 7 ETM+, and Landsat 8 OLI acquired in 1996, 2006, and 2016 respectively. For each image, a supervised classification method was performed using open source GRASS GIS software. The resulting maps were then compared to quantify the changes. Field work activity conducted and confirmed the changes that occurred in the study areas. Our study shows that all of the two districts exhibit successfulness of the plantation program. Ground truth survey confirmed that the successfulness of the plantation program is due to the participation of communities in the area of study. This study also shows that by using open source software and freely available satellite images, the fast, robust, and reliable data as an initial step to monitor both short-term and long-term plantation program can be collected effectively and inexpensively.
The appearance of rabbit skin tissue (Oryctolagus cuniculus) after supplementation of Aloe vera and Spirulina fusiformis YASMI PURNAMASARI KUNTANA; HUSMY YURMIATI; ASRI PENI WULANDARI; FARIDA SYAFITRI; RUHYAT PARTASASMITA
Nusantara Bioscience Vol. 9 No. 3 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090305

Abstract

Kuntana YP, Yurmiati H, Wulandari AP, Syafitri F, Partasasmita R. 2017. The appearance of rabbit skin tissue (Oryctolagus cuniculus) after supplementation of Aloe vera and Spirulina fusiformis. Nusantara Bioscience 9: 268-274. The research is about the appearance of rabbit skin tissue (Oryctolagus cuniculus L.) after supplementation of Aloe vera L. and Spirulina fusiformis Vor. has been done. This study was carried out to find the most effective formulation ofsupplement of A. vera and S. fusiformis as a natural supplement that can generate the best appearance of rabbit skin tissue. The method in this research was a single Complete Random Design (CRD) on 28 male New Zealand White strains rabbits with the age of 16 weeks. The treatments were divided into seven groups with four replications, namely, P0 (negative control ), P1(positive control, vitamin C19 mg/kg BW), P2 (A. vera 74 mg/kg BW), P3 (S. fusiformis 296 mg/kg BW), P4 (A. vera: S. fusiformis, 74: 148 mg/kg BW), P5 (A. vera: S. fusiformis, 74: 296 mg/kg BW), and P6 (A. vera: S. fusiformis, 74: 593 mg/kg BW). The parameters observed were production aspect (hair texture, slaughter weight, skin width, and skin weight percentage) and histological aspect (thickness of skin tissue and the amount of hair follicle). All of the data was analyzed using ANOVA test (P ˃ 95%) and Duncan test (P ˃ 95%). The result showed that the giving of supplement with the basis of the formulation of A. vera 74 mg/kg bw and S. fusiformis 296 mg/kg bw was effective in generating the best appearance of rabbit skin tissue.
Development of shrimp shells-based compost and plant-based pesticide using bio-activators from Golden Apple Snails and their effects on the kenaf plant growth and pest population RUSMINI RUSMINI; RIAMA RITA MANULLANG; DARYONO DARYONO
Nusantara Bioscience Vol. 9 No. 3 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090304

Abstract

Rusmini, Manullang RR, Daryono. 2017. Development of shrimp shells-based compost and plant-based pesticide using bioactivators from Golden Apple Snails and their effects on the kenaf plant growth and pest population. Nusantara Bioscience 9: 260-267. Kenaf (Hibiscus cannabinus L) cultivation generally uses chemical fertilizers and pesticides to increase production despite the adverse effects of those chemicals toward environmental ecosystem. Meanwhile, there are a lot of unutilized wastes produced from agriculture and fishery. This study aimed to develop the best bio-activator from Golden Apple Snails and spices to decompose the shrimp shells waste. The bioactivator was utilized for the production of plant-based pesticide to control pests in kenaf plant, and shrimps-shells-based composts to improve kenaf plant growth. This study employed a Randomized Block Design with two factorials. The first factor was the shrimp shells-based composts (K) which consist of three levels, and the second factor was the plant-based pesticide (P) which also consists of three levels. Each of experiment was repeated twice. Thus, in total there were 18 treatments. The shrimp shells-based composts had a pH value of 8.79, Potassium (K) 8.13 %, organic carbon (C) 17.45%, Nitrogen (N) 3.62%, Phosphorus (P) 2.27%; Magnesium (Mg) 0.59%, and Calcium (Ca) 7.64%; and a C/N ratio of 4.82. Kenaf plant height at 6 and 9 weeks after planting (WAP) showed significant differences upon the shrimp shells-based composts treatments. At 6 weeks after planting, kenaf plant diameter showed significant differences upon the composts treatment, while at 9 weeks after planting, the plant diameter indicated significant differences upon both the compost and plant-based pesticide applications. Pest populations that were present in kenaf plants were caterpillars, bugs, and beetles.
Evaluation of orange-fleshed sweetpotato genotypes for yield and yield contributing parameters in two environments WIWIT RAHAJENG; ST. A. RAHAYUNINGSIH
Nusantara Bioscience Vol. 9 No. 3 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090306

Abstract

Rahajeng W, Rahayuningsih SA. 2017. Evaluation for yield and yield contributing parameters of orange-fleshed sweetpotato genotypes in two environments. Nusantara Bioscience 9: 275-281. Orange-fleshed sweetpotatoe (OFSP) is one of the most important sources of betacaroten and carbohydrates. OFSP showed varying responses to different environments, depending on the adaptability of the genotypes. The objective of this study was to evaluate the correlation between yield and yield component of OFSP genotypes at two different agroclimatological environments (Mojokerto and Blitar) from May to September 2013. Twenty four OFSP genotypes (twenty two clones and two varieties as the check) were used in this study and arranged in a randomized complete block design with three replications. Results showed that responses of OFSP genotypes to the environments varied and genotypes had highly significantly affected all parameters. Based on the criteria of high yield, amylum content, and dry matter production four clones were selected (MSU 09008-54, MSU 07009-113, MSU 07025-45, and MSU 09025-71) from Blitar and two clones (MSU 07009-113 and MSU 070-64) from Mojokerto. Clone MSU 07009-113 showed good performance in both environments for yield, amylum content, and dry matter production parameters. The result of the correlation analysis reveals that root yield is highly significant and positively correlated with dry matter production, number of roots, diameter of root, and harvest index in two locations (Blitar and Mojokerto). Based on the correlation and path analysis, dry matter production, root diameter, and harvest index can be used as an effective indicators selection for high yield OFSP genotypes.
Isolation of anti-idiotype minor capsid Human papillomavirus type 16 (HPV 16 L2) IgY from egg yolk as immunogen of HPV vaccine EKA NONENG NAWANGSIH; SAYU PUTU YUNI PARYATI; JUSUF S. EFFENDY; SUNARJATI SUDIGDOADI; EDHYANA SAHIRATMADJA; DIKI HILMI; EVI SOVIA
Nusantara Bioscience Vol. 9 No. 3 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090307

Abstract

Nawangsih EN, Paryati SPY, Effendy JS, Sudigdoadi S, Sahiratmadja E, Hilmi D, Sovia E. 2017. Isolation of anti-idiotype minor capsid Human papillomavirus type 16 (HPV 16 L2) IgY from egg yolk as immunogen of HPV vaccine. Nusantara Bioscience 9: 282-287. A potential strategy for the production of safe protective vaccines for human papillomavirus (HPV) infection is to utilize antiids. Meanwhile, hyperimmunized hens could provide a convenient of specific immunoglobulin in their yolks (IgY). This study aimed to isolate IgY specific to anti-idiotype HPV 16 L2 from egg yolk which could be used for future alternative immunogen for HPV vaccine. Antibody anti-idiotype derived from chicken egg yolk that chickens immunized by HPV 16 L2 antibody. The antibodies were purified from immunized chicken egg yolk, then electrophoresis it using SDS-PAGE method to determine the molecular weight of IgY. To determine an amount of protein, we used fluorometer method and to confirm an existence of specific IgY using ELISA method. The results showed that the IgY preparation dissociated into three protein major bands with molecular weights of 180; 65; and 25 kD. The IgY and IgY specific concentration in eggs yolk increased until 8th week. After 8th week the levels decreased gradually. Samples derived from chickens immunized showed significantly higher concentrations of IgY and IgY specifics than control (p<0.05). These results suggested that chicken IgY could be a practical strategy in large-scale production of specific antibody anti-idiotype HPV 16 L2 for HPV Vaccine.
Yield and Zn content of biofortified rice genotypes in an Indonesian rice agro-ecosystem UNTUNG SUSANTO; UMI BAROKAH; AKHMAD HIDAYATULLAH; SATOTO SATOTO; MALIKARJUNA SWAMY
Nusantara Bioscience Vol. 9 No. 3 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090308

Abstract

Susanto U, Barokah U, Hidayatullah A, Satoto, Swamy M. 2017. Yield and Zn content of biofortified rice genotypes in an Indonesian rice agro-ecosystem. Nusantara Bioscience 9: 288-294. Approximately one-third of the world's population suffer from Zn deficiency causing significant socio-economic losses as a result of stunting and compromised immune system function. One strategy to overcome the problem is by developing rice cultivars with high grain Zn content (Zn Rice) to improve dietary intake.This study reports the yield and Zn content of 22 rice genotypes developed at the International Rice Research Institute (IRRI) and the Indonesian Centre for Rice Research (ICRR).The experiment was conducted in the Cirebon district of West Java province during the 2013 wet season (2013WS). Yield and grain Zn content (using an XRF machine) were measured. Five lines demonstrated higher yield (ranging from 7.0 to 8.9 t/ha) than the check variety Ciherang (5.2 t/ha), but had similar grain Zn content to the check variety Ciherang (23.4 ppm), ranging from 19.0 to 24.8 ppm. On the other hand, seven lines had higher grain Zn content (ranging from 30.0 to 34.2 ppm) compared to Ciherang, and five of these lines had comparable yield to Ciherang. The selected lines had acceptable agronomic traits, and are suitable for further testing and utilization, in addition to providing a foundation for future improvement in the dual goals of increasing the yield and nutritional value of rice.
Short Communication: Augmentation of cardioprotective effect of captopril by Costus speciosus against isoproterenol induced myocardial toxicity in rats ABDULRAHMAN A.I. AL-YAHYA; MOHAMMED ASAD; ABDULMONIEM SADABY; KHALID ELFAKKI IBRAHIM
Nusantara Bioscience Vol. 9 No. 3 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090309

Abstract

Al-Yahya AAI, Asad M, Sadaby A, Ibrahim KE. 2017. Short Communication: Augmentation of cardioprotective effect of captopril by Costus speciosus against isoproterenol induced myocardial toxicity in rats. Nusantara Bioscience 9: 295-299. This study determined the pharmacodynamic interaction of captopril, a known cardioprotective effect with methanolic extract of Costus speciosus rhizomes (Costaceae) that is known for its powerful antioxidant action during isoproterenol induced cardiac toxicity in rats. A methanolic extract of the rhizomes was prepared by maceration. Rats were administered the methanolic extract at two different doses of 200 mg/k or 400 mg/kg orally and captopril was administered orally at a dose of 30 mg/kg. All the drugs alone or in combination were once daily for two weeks. At the end of treatment period, two doses of isoproterenol (150 mg/kg, s.c) were administered to rats at 24 hr interval. Blood was withdrawn to estimate creatinine kinase-MB (CK-MB) activities. The heart tissue was subjected to histological examinations to determine the extent of damage. Isoproterenol induced severe damage to the myocardium that was indicated through an elevation in serum CK-MB activity and the same was confirmed by histological examinations. Costus speciosus at both the tested doses attenuated the damage produced by isoproterenol. Both the doses caused a decrease in the biomarker activity as well as reduced the myocardial damage as observed in histological examination. A similar effect was observed with captopril. The co-administration of captopril with either dose of Costus speciosus demonstrated excellent cardioprotection suggesting that combination of this herb with captopril augments its cardioprotective action. It was concluded that Costus speciosus shows dose-dependent cardioprotection and augments the cardioprotective effect of captopril during isoproterenol induced cardiotoxicity in rats.
Microbe-enriched compost application on germination substrates of Beilschmiedia roxburghiana, Bouea oppositifolia and Syzygium polycephalum HENDRA HELMANTO; FRISCA DAMAYANTI; DIAN LATIFAH
Nusantara Bioscience Vol. 9 No. 3 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090310

Abstract

Helmanto H, Damayanti F, Latifah D. 2017. Microbe-enriched compost application on germination substrates of Beilschmiedia roxburghiana, Bouea oppositifolia and Syzygium polycephalum. Nusantara Bioscience 9: 300-305. The success of germination has been an important issue in many forest restoration programs; that is mainly affected by the quality of sowing media. Application of Bioposka compost (microbe-enriched compost) in the sowing media was hypothesized to increase the success of the germinating and transplanting also. The research aimed at investigating the effect of Bioposka compost to the germination and the seedling growth of the study species. The complete-randomized research design was used for three sowing media, i.e., sand, Bioposka compost and the mixture of sand: compost (1: 1 ratio) of tree species i.e., Syzygium polycephalum, Bouea oppositifolia and Beilsmedia roxburghiana. The variables observed were total and normal germination capacity, first germination, final germination, germination rate, germination simultaneity and seedling growth. The results were analyzed using STAR (Statistical Tool for Agricultural Research). In addition, the microbial abundance of bacteria, fungi, and yeast in each media were calculated using the spread-plate methods. The germination and seedling growth responses were varied between the different media. The germination capacity and seedling growth of Syzygium polycephalum and Bouea oppositifolia were lower by microbe-enriched compost. By contrast, the germination capacity and the seedling growth of Beilsmedia roxburghiana were not significantly different. Moreover, the microbe-enriched compost application increased the abundance of bacteria, fungi, and yeast in the media.

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