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Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 3 Documents
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Search results for , issue "Vol. 16 No. 1 (2022): March" : 3 Documents clear
Optimization of Rhizopus spp. Production as Mycoprotein using Soymilk Media Dani Muliawan Halim; Anastasia Tatik Hartanti; Stephanie
Microbiology Indonesia Vol. 16 No. 1 (2022): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.032 KB) | DOI: 10.5454/mi.16.1.23-29

Abstract

Mycoprotein is food with high protein content, fiber, and low in cholesterol made from fungal mycelium. In this research, mycoprotein was produced by spp. isolated fr Rhizopus om tempeh with soymilk as growth media. This research aims to determine the best strain of spp. and op Rhizopus timum carbon to nitrogen ratio for mycoprotein production Two parameters were applied which w . ere inoculum selection and carbon to nitrogen ratio treatment in media. The best inoculum was selected from four strains of Rhizopus spp., ATH 1,ATH 24,ATH  40, and ATH 53. On the other hand, carbon to nitrogen ratio treatment used were as follows 20:1, 20:2, and 40:2. Mycelium dry weight and protein content were measured, as well as reduction sugar, dissolved protein and total volatile base nitrogen concentration in media. The best strain for producing biomass was ATH 24 with 0.6 g of 0 mycelium dry weight per 50 mL of media and the protein content was 0.236 g. The best carbon to nitrogen ratio treatment was 20:1 with 0.57 gram of mycelium dry weight per 50 mLof media and the protein content was 0.20 g. Thus, our data indicate that strain ATH 24 with 20:1 of carbon to nitrogen ratio in media were highly potential for producing mycoprotein. Keywords: fermentation, mycelium, mycoprotein, Rhizopus, soymilk
Lipase Activity Enhancement of KC4J Mutant from Oil Palm Waste Using Response Surface Method and Characterization. Aris Indriawan; Trismilah .; Wibowo Mangunwardoyo; Dadang Suhendar
Microbiology Indonesia Vol. 16 No. 1 (2022): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.621 KB) | DOI: 10.5454/mi.16.1.1-12

Abstract

Lipase has an important role in industry. The KC4J mutated isolates from oil palm waste had 100% similarity to Aspergillus fumigatus strain RA204, a fungus known produce lipase. The study aimed to increase lipase activity of KC4J mutant through media optimization using Response Surface Methodology (RSM) and partial characterization. The three variables of media composition (olive oil, soy flour, and pH were optimized using Central Composite Design (CCD). The lipase characterization measured the influence of pH, temperature and metal ions. The pH tested on range 6 to 12, while the temperature variation tested on 30 to 70 °C. The metal ions tested were Mg2+, Ca2+, Zn2+, Mn2+, Fe2+ and K+ with concentrations of 1 mM and 10 mM. The production medium containing 1.25% of olive oil, 3.5% of soy flour and 7.5 pH resulting 11.25 U/mL of Lipase activity, which was higher than the previous media composition (10.00 U/mL). The results of CCD and quadratic analysis showed that the source of carbon, nitrogen and pH had an effect on lipase activity which showed R2 0.93. The optimum lipase activity produced at pH 6 and on 60 °C, and the lipase stable at pH 6-8 and on 30-70 °C. All metal ions tested were able to increase lipase activity with Ca 2+ ion gave the highest result. Keywords: Lipase, KC4J mutant, Central Composite Design, Oil Palm Waste.
Pretreated Sugarcane Bagasse Result in more Efficient Degradation by Streptomyces sp S2 Stanislaus Aditya Agung; Rismawati .; Dede Heri Yuli Yanto; Anja Meryandini; Titi Candra Sunarti
Microbiology Indonesia Vol. 16 No. 1 (2022): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (431.562 KB) | DOI: 10.5454/mi.16.1.13-22

Abstract

Streptomyces genera plays an important role in lignocellulose degradation. Many research found that Streptomyces have cellulolytic and ligninolytic enzymes that are sufficient to degrade lignocellulosic materials. However, a minimum lignocellulosic material condition that can efficiently be degraded by sp. has Streptomyces not been fully understood. In this research, three pretreatment conditions (physical, alkaline-hydrothermal, and hydrogen-peroxide chemical treatments) of sugarcane bagasse were used as lignocellulosic material to be further degraded by Streptomyces sp. S2. Lignocellulose component measurement concluded that raw (physically treated only) bagasse was not efficiently degraded by  Streptomyces sp S2. Hydrogen-peroxide was effective in reducing both syringic and guaiacyl lignin. Meanwhile, alkaline-hydrothermal pretreatment was very effective in lowering syringic lignin. This study suggests that hydrogen-peroxide pretreatment can be used in any type of lignocellulosic material, which can be further degraded by Streptomyces sp. S2. On the other hand, alkaline hydrothermal pretreatment is best suited to degrade lignocellulosic material with a high percentage of syringic lignin. Key words: Alkaline-hydrothermal treatment, Hydrogen peroxide treatment, lignocellulose, Streptomyces sp. S2, sugarcane bagasse

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