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All Journal Microbiology Indonesia ANNALES BOGORIENSES Indonesian Fisheries Research Journal Jurnal Ilmu Kefarmasian Indonesia
Wibowo Mangunwardoyo
Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Kapang Endofitik Tanaman Brucea javanica (L.) Merr yang Berpotensi menghasilkan Enzim Xilanase Wibowo Mangunwardoyo; Shirly Kumala; I. G.A. Ayu Wiratih
JURNAL ILMU KEFARMASIAN INDONESIA Vol 4 No 1 (2006): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Xylanase enzyme activity of endophytic fungi from Brucea javanica (L.) Merr has been done. Fruit, leave and stem of the plant collected from Bogor, Cianjur, and Tawangmangu which used in this study. To evaluate the enzyme activity the endophytic fungi isolate were inoculated on PDY medium and fermented at room temperature, shake at 150 rpm for 14 days. The supernatan were used to examine the extracellular enzyme and the biomass for intracellular enzyme. The DNS methods was used to test the enzyme activity. The results showed isolate 1.1.8; 1.2.17; 1.3.16 and 1.3.6 produced intra and extracellular xylanase enzyme. Isolate 1.1.6 produced only extracellular enzyme while isolate 1.2.2 did not produce both intra and extracellular enzyme.
Fermentasi Diam dan Goyang Isolat Kapang Endofit dari Brucea javanica L. Merr. dan Uji Aktivitas Antimikroba Shirly Kumala; Wibowo Mangunwardoyo; Peny Budiarti
JURNAL ILMU KEFARMASIAN INDONESIA Vol 3 No 2 (2005): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Exploring an antibiotic from microorganism is potential to discover the new antibiotic. The endophytic microorganism is one of the minimanufacturer producing the secondary metabolite such as antibiotic. Screening on the endophytic molds isolated from Brucea javanica L. Merr has been carried out. Fermentation in still and shake culture were used to produce the antibiotic using PDY medium. Antimicrobial activity was assayed using diffusion agar method. The results revealed that shake culture gave a better antimicrobial activity than still culture. Amount of eight isolate studied showed microbial activity against Gram positive bacteria: Staphylococcus aureus and Bacillus subtilis also Gram Negative bacteria: Escherichia coli and Salmonella typhi, however no microbial activity recorded againts Candida albicans. Isolate number 1.2 gave the highest antimicrobial activity on still culture and isolate number 1.7 gave the highest antimicrobial activity on shake culture. Further research should be carried out on identification and purification of the antimicrobial agent.
Uji Aktivitas Enzim Xilanase Ekstraselular dan Intraselular Bakteri Endofitik Tanaman Brucea javanica (L.) Merr. Shirly Kumala; Wibowo Mangunwardoyo; Diah Dethriani
JURNAL ILMU KEFARMASIAN INDONESIA Vol 4 No 2 (2006): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Assay for xylanase enzyme activity of endophytic bacteria from Brucea javanica (L.) Merr. has been carried out. Parts of the plants used in this study were stem, leave and fruit. Samples were collected from Bogor, Cianjur and Tawangmangu. To evaluate the enzyme activity the fungi were shakefermented for 14 days. The supernatant were used to examine the extracellular enzyme and biomass for intracellular enzyme, using DNS methode to test the activity of enzyme. The results showed that from ten isolates only four isolates produced xylanase enzyme. Isolate 2.3.1 and 2.3.6 produced extra and intracellular enzyme, while isolate 2.1.15 and 2.3.13 only produced extracellular xylanase enzyme.
Ekstraksi dan Identifikasi Senyawa Antimikroba Herba Meniran (Phyllanthus niruri L.) WIBOWO MANGUNWARDOYO; ENI CAHYANINGSIH; TEPY USIA
JURNAL ILMU KEFARMASIAN INDONESIA Vol 7 No 2 (2009): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Phyllanthus niruri L. herbs was extracted using ethanol 96%, ethylacetate and n-hexane. The rendements were respectively 49.25% (for ethanol extract), 4.18% (ethylacetate extract) and0.78% (n-hexane extract). Identification of the ethanol extract using phytochemical assays resulted in alkaloid, flavonoid, saponin, and tannin compounds. Purification of the ethanol extract using thin layer chromatography (TLC) with n-hexane-ethylacetate (6:4) followed by the inhibitory activity test using bioautography TLC showed that the spot of Rf 0,46 inhibited Staphylococcus aureus and that of Rf 0,66 inhibited Candida albicans. Analysis with infra red spectrophotometer showed that the ethanol extract has hydroxyl (-OH) and carbonyl (C=O) functional groups.
ANTIBACTERIAL TEST OF PANGIUM (Pangium edule Reinw) EXTRACT AGAINST THE GROWTH OF FISH SPOILAGE BACTERIA Endang Sri Heruwati; Lily lsmaini; Wibowo Mangunwardoyo
Indonesian Fisheries Research Journal Vol 15, No 1 (2009): (June 2009)
Publisher : Research Center for Fisheries

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8086.247 KB) | DOI: 10.15578/ifrj.15.1.2009.65-73

Abstract

An antibacterial test was conducted on fresh and fermented Pangium edule Reinw. Seed extracts against gram positive and negative spoilage bacteria associated with fish.
Improvement of Endoglucanase Activity in Penicillium oxalicum ID10-T065 Mutated by Ultra Violet Irradiation and Ethidium Bromide Asnany Caniago; Wibowo Mangunwardoyo; Sukma Nuswantara; Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.811 KB) | DOI: 10.14203/ann.bogor.2015.v19.n2.27-38

Abstract

Penicillium sp. is known as filamentous fungi that produce complete cellulase. Cellulase. This study aims to improve endoglucanase activity of Penicillium oxalicum ID010-T065 by mutated with ultra violet irradiation (with dose of 0.1 J/cm2, 15 cm), ethidium bromide (10 µg/mL, 1 hour) and combination of both mutagens. The endoglucanase activity of all mutants was higher than that of the wild type (1.03 U/mL). Mutant UVEB-42 exposed to combine mutation showed the highest endoglucanase activity (2.76 U/mL) with a 2.70 fold increase. Mutant EB-45 (1.83 U/mL) exposed to ethidium bromide solution showed a 1.8 fold increase. Mutant UV-13 (1.72 U/mL) exposed to UV irradiation for 3 minutes showed a 1.7 fold increase. All mutants have optimum endoglucanase activity at 50 °C. Mutant UVEB-53 showed the highest thermostability by retaining 86 % of endoglucanase activity at 90 °C. The gene analysis of the endoglucanase I gene showed 3 bases mutated at mutant UV-13 and UVEB-53 that changed proline to serine. Mutant EB-45 showed 4 bases mutated that changed valine to glysine and proline to serine. Two bases mutated at Mutant UVEB-53 changed proline to serine. Bases mutated in eg1 gene could influenced the enhance of enzym activity in mutant.
Diversity and Antimicrobial Activity of Lichens-Associated Actinomycetes in Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) Indonesia Agustina Eko Susanti; Shanti Ratnakomala; Wibowo Mangunwardoyo; Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (945.612 KB) | DOI: 10.14203/ann.bogor.2019.v23.n1.1-12

Abstract

    Bioprospecting has developed to all biological taxa including procaryotic. Actinomycetes become interesting procaryotic because of the ability to produce important secondary metabolite for human life. Actinomycetes are known as the largest antibiotic producer that has a broad range habitat. Some research has been done to find new antibiotic from the various habitat of actinomycetes. One of the interesting habitats of actinomycetes which never been explored in Indonesia is lichens... Lichens as the symbiotic structure of alga and fungi areknown as the ecological niche of various kinds of microorganisms including actinomycetes. Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) have various species of trees as the habitat of lichens. These areas are known as one of the research locations to explore the biodiversity of Indonesia. The aims of this research is to study the diversity and antimicrobial potency of actinomycetes isolated from 10 lichen samples with various type of thallus; crustose, fructose and foliose. Lichen samples were grown on the bark of 9 trees species in CSC and CBG. Isolation process used three agar media; HV, YIM6 and YIM711 with cycloheximide and nalidixic acid. Molecular identification based on 16S rRNA gene sequence. Antimicrobial activity was tested to 65 isolates by agar diffusion method to Bacillus subtilis BTCC B.612, Escherichia coli BTCC B.614, Candida albicans BTCC Y.33, Staphylococcus aureus BTCC B.611, Micrococcus luteus BTCC B.552. Isolation process retrieved 125 isolates with the highest number grow on HV agar medium. Based on the sample, the highest number of actinomycetes were isolated from crustose lichen attached on the bark of Averrhoea carambola. A total 69 isolates were identified as the genera Actinoplanes, Amycolatopsis, Angustibacter, Kribbella, Micromonospora, Mycobacterium, and Streptomyces. The screening process showed 24 isolates have antimicrobial activity, with the highest inhibitory activity against Micrococcus luteus BTCC B.552.
Enhancement of β-Glucosidase Activity in Penicillium sp. by Random Mutation with Ultraviolet and Ethyl Methyl Sulfonate Vilya Syafriana; Sukma Nuswantara; Wibowo Mangunwardoyo; Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.422 KB) | DOI: 10.14203/ann.bogor.2014.v18.n2.27-33

Abstract

The genus Penicillium has a potential ability to produce β-glucosidase. The aim of the study was to improve the β-glucosidase activity of Penicillium sp. ID10-T065 with physical (Ultraviolet = UV), chemical (Ethyl Methyl Sulfonate = EMS), and combined mutation (UV-EMS). The spores of Penicillium sp. ID10-T065 were exposed into UV irradiation for 3 minutes with dose of 0.1 J/cm2 and 13 cm of distances. Chemical mutation was done by treated spores into 3% of EMS solution for an hour. Combined mutation of UV and EMS were also performed by UV for 3 minutes (0.1 J/cm2, 15 cm) and continued with soaking into 2-3% of EMS solution. The developed mutants were screened, selected and assayed. Comparison of enzyme activities with the wild- type (1.78 U/ml), mutant UV13 (5.53 U/ml) showed a 3.1 fold increase; mutant EM31 (4.26 U/ml) showed a 2.4 fold increase. Meanwhile, mutant UM23 obtained from the multiple exposures showed a decreased activity (1.75 U/ml). Mutant UV13 showed the best enzyme activity to be considered as a potential strain for β-glucosidase producer. This result needs to be further elaborated especially on its genetic stability studies in order for the ascertained as a stable mutant.
Lipase Activity Enhancement of KC4J Mutant from Oil Palm Waste Using Response Surface Method and Characterization. Aris Indriawan; Trismilah .; Wibowo Mangunwardoyo; Dadang Suhendar
Microbiology Indonesia Vol. 16 No. 1 (2022): March
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.621 KB) | DOI: 10.5454/mi.16.1.1-12

Abstract

Lipase has an important role in industry. The KC4J mutated isolates from oil palm waste had 100% similarity to Aspergillus fumigatus strain RA204, a fungus known produce lipase. The study aimed to increase lipase activity of KC4J mutant through media optimization using Response Surface Methodology (RSM) and partial characterization. The three variables of media composition (olive oil, soy flour, and pH were optimized using Central Composite Design (CCD). The lipase characterization measured the influence of pH, temperature and metal ions. The pH tested on range 6 to 12, while the temperature variation tested on 30 to 70 °C. The metal ions tested were Mg2+, Ca2+, Zn2+, Mn2+, Fe2+ and K+ with concentrations of 1 mM and 10 mM. The production medium containing 1.25% of olive oil, 3.5% of soy flour and 7.5 pH resulting 11.25 U/mL of Lipase activity, which was higher than the previous media composition (10.00 U/mL). The results of CCD and quadratic analysis showed that the source of carbon, nitrogen and pH had an effect on lipase activity which showed R2 0.93. The optimum lipase activity produced at pH 6 and on 60 °C, and the lipase stable at pH 6-8 and on 30-70 °C. All metal ions tested were able to increase lipase activity with Ca 2+ ion gave the highest result. Keywords: Lipase, KC4J mutant, Central Composite Design, Oil Palm Waste.
Co-Authors Agustina Eko Susanti Aris Indriawan Asnany Caniago Dadang Suhendar Diah Dethriani Endang Sri Heruwati ENI CAHYANINGSIH I. G.A. Ayu Wiratih Lily lsmaini Peny Budiarti Puspita Lisdiyanti Puspita Lisdiyanti Puspita Lisdiyanti Shanti Ratnakomala Shirly Kumala Sukma Nuswantara Sukma Nuswantara Syafriana, Vilya TEPY USIA Trismilah .