cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Iman Rusmana
Contact Email
rusmana13@yahoo.com
Phone
+62217560536
Journal Mail Official
microbiology.indonesia@gmail.com
Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
Location
Kota tangerang,
Banten
INDONESIA
Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 6 Documents
 1 
Search results for , issue "Vol. 9 No. 4 (2015): December 2015" : 6 Documents clear
Isolation, Identification and Screening of Antimicrobial Properties of the Marine-Derived Endophytic Fungi from Marine Brown Seaweed CHANGI WONG; PETER PROKSCH; LEE TUNG TAN; SAMUEL LIHAN; AAZANI MUJAHID; MORITZ MÃœLLER
Microbiology Indonesia Vol. 9 No. 4 (2015): December 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (553.03 KB) | DOI: 10.5454/mi.9.4.1

Abstract

Marine seaweeds are known to produce valuable medicinal compounds such as antioxidants and anticoagulants, and have been reported to display antimicrobial activity against gram positive and gram negative bacteria. Several studies have identified so-called endophytic fungi living inside their hosts as the source of active compounds. In this study marine brown seaweed, Padina sp., was studied with regards to their endophytic fungi to assess if they are the source of the reported antimicrobial activity. Twenty fungal isolates were isolated from Padina sp. collected off Talang-Talang Island, Sarawak, Malaysia. All isolates were screened for their antimicrobial properties and 11 out of 20 isolates displayed positive results. DNA was successfully extracted for five isolates and sequence analysis grouped all of them with other endophytic fungi. “Fungus 2” seems to be related to a so far uncultured endophytic fungus. “Fungus 19” showed the most promising antimicrobial properties and was chosen for further agar well assay and cytotoxicity testing. Its ethyl-acetate extract showed positive results in the agar well assay and also a cytotoxic effect on Artemia nauplii. The extract was screened using HPLC and showed a compound similar to a known anti-cancer compound, dihydromyricetin, which is also an anti-intoxicant, anti-inflammatory and anti-oxidative agent which may be responsible for the observed antimicrobial activity.
Antibiotic Use Is Not a Risk Factor of Infection by Extended-Spectrum Beta-Lactamase Producing Bacteria in Dr. Soetomo Hospital Surabaya
Microbiology Indonesia Vol. 9 No. 4 (2015): December 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (483.637 KB) | DOI: 10.5454/mi.9.4.2

Abstract

Infection by Extended-Spectrum Beta-Lactamase (ESBL) producing bacteria confers a major challenge for clinicians due to limited treatment options and poor prognosis. Inappropriate antibiotic use is thought to cause the emergence of this resistant strain through selective pressure mechanisms. This study aims to describe the proportion of ESBL-producing bacteria and characteristics of patients with ESBL-producing bacterial infection, and to analyze the risk factors of infection by ESBL-producing bacteria in Dr. Soetomo Hospital, Surabaya. A cross-sectional study was conducted on medical records of inpatients of Internal Medicine Ward of Dr. Soetomo Hospital. Samples were classified into ESBL-positive or ESBL-negative groups. Demographic data, clinical data and previous antibiotic use of 66 samples (33 in each group) were retrospectively obtained. As many as 30 patients (45.5%) were male. Mean age of patients in the ESBL-positive and negative group were 53.57 (±16.77) and 54.27 (±14.88) years, respectively (p>0.05). The median pre-infection length of stay was 4 and 3 days for ESBL-positive and negative group, respectively (p>0.05). Type 2 diabetes mellitus was the most common comorbid disease (33.3%). The most frequent bacteria obtained from clinical isolates was Escherichia coli (49.3%). Proportion of ESBL producers amongst E.coli and K. pneumoniae isolates were 75% and 38.5%, respectively. The most frequently prescribed empirical antibiotic was ceftriaxone. None of the antibiotic used were risk factors for infection by ESBL-producing bacteria. Although none of the assessed variables were risk factors for ESBL-infection was discovered, this study finds a significantly larger proportion of ESBL-E. coli compared to non-ESBL producing E. coli. Further studies should include larger sample size and quantitatively measured antibiotic use.
Orchid Mycorrhizal Fungi: Identification of Rhizoctonia from West Kalimantan ROSA SURYANTINI; REINE SUCI WULANDARI; RINA SRI KASIAMDARI
Microbiology Indonesia Vol. 9 No. 4 (2015): December 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2256.142 KB) | DOI: 10.5454/mi.9.4.3

Abstract

Orchid is an ornamental plants with high economic value. The excessive exploitation of orchids threatened or even endangered the species, especially those of the epiphytic orchids (Appendiculla sp., Calanthe vestita, and Bulbophyllum beccarii) in West Kalimantan. The discovery of the interaction between orchids and mycorrhizal fungi raises the possibility of ex situ conservation of orchids and it will ensure the success of orchid conservation. Orchid mycorrhizal fungi belongs to the group of Rhizoctonia-like,in which comprised of different genus such as Ephulorhiza, Ceratoriza, and Tullasnela. So far, there is no report on the identit of orchid mycorrhiza associated with the epiphytic orchids in West Kalimantan. The purpose of this study was to identify Rhizoctonia-like associated with Appendiculata sp., Calanthe vestita, and Bulbophyllum beccarii roots in the forest of Raya Pasi and Gunung Bawang, West Kalimantan. The methods were isolation and identification of Rhizoctonia-like from healthy orchid's root based on their morphological characteristics (such as the colony colour, hyphal cell size, sclerotial, concentric circles and monilioid cell, number of nuclei per cell), observation of peloton in root tissue and grouping of isolates. Based on identification of orchid mycorrhiza on the roots of the three species of orchids from West Kalimantan, it was observed that Ceratorhiza sp. was associated with Appendiculla sp.,Ephuloriza sp. with C. vestita, and Tullasnela sp. with B. beccarii roots, respectively. This result is preliminary information and it is still need to be further studied, especially on the role of Rhizoctonia-liker as orchid mycorrhizal fungi in association with the epiphytic orchid for conservation. 
Heavy Metals Biosorption by Copper Resistant Bacteria of Acinetobacter sp. IrC2 WAHYU IRAWATI; ADOLF JN PARHUSIP; NIDA SOPIAH
Microbiology Indonesia Vol. 9 No. 4 (2015): December 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2817.263 KB) | DOI: 10.5454/mi.9.4.4

Abstract

 Heavy metal pollution is a serious problem because it cannot be degraded by natural processes and persist in soil, water, and sediment. Many indigenous microorganisms isolated from heavy metal contaminated sites had tolerance to heavy metals toxicity and could be used for bioremediation agent because of its capability to biosorb heavy metals. The aim of this research was to study the potency of copper resistant bacteria Acinetobacter sp. IrC2 as a biosorbent of heavy metals. Biosorption was determined by measuring the heavy metals concentration on growing medium by using atomic absorption spectrophotometer. The research showed that Acinetobacter sp. IrC2 was capable of growing in medium containing each of 2 mM of copper, zinc, lead, cadmium, and the mixture of those heavy metals. The addition of copper and lead in the medium changed morphological appearance of colonies to green and brown, respectively, suggesting that the survival mechanism of the isolate was by biosorbing copper or lead inside the cells. The percentage of heavy metals biosorption efficiency using live cells of Acinetobacter sp.IrC2 were up to 64.31% of copper, 24.73% of zinc, 62.79% of lead, and 11.56% of cadmium. Acinetobacter sp. IrC2 also reduced copper, lead, and cadmium concentration up to 24.30, 75.93, and 16.38%, respectively, in medium supplemented with 1 mM of the mixture of these heavy metals. The findings of this study indicated that Acinetobacter sp. IrC2 was a promising bacterium for removal of heavy metals.
Screening, Purification, and Characterization of Cellulase from Fungi Isolated from Used Mushroom Substrate IWAN SASKIAWAN; NUR HASANAH
Microbiology Indonesia Vol. 9 No. 4 (2015): December 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (834.134 KB) | DOI: 10.5454/mi.9.4.5

Abstract

Alarge number of microorganism especially filamentous fungi has ability to degrade cellulose. The purpose of this study was to conduct screening, purification, and characterization of cellulase from fungi which was isolated from used paddy straw mushroom substrate. Screening of cellulytic activity using CMC medium shown that 11 out of 20 isolates of fungi produced a clearing zone surrounding fungal colony. Among them isolate number JMF 12 showed the highest cellulase activity and was further used for purification and characterization. The cellulase was purified to electrophoretical homogeneity by ammonium sulfate precipitation, dialyzed by Novagen D-Maxi TubeTM Dialyzer, and Sephadex G-100 gel filtration chromatography. The recovery and purification fold was 3.82 % and 1.98 respectively, after Sephadex G-100 gel filtration chromatograph. The o purified cellulase had an optimal pH and temperature at 6 and 45 C. The Km and Vmax of cellulase was 11.43 mM and 0.006mmol/min respectively. The purified cellulase was activated by Na+ and Zn++ but inhibited by Ca++, Co++, Fe++, and  Hg++ .
ITA registration form and back cover
Microbiology Indonesia Vol. 9 No. 4 (2015): December 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1786.41 KB) | DOI: 10.5454/mi.9.4.%p

Abstract

Page 1 of 1 | Total Record : 6

 1 

Filter by Year

2015 2015


Reset
Filter By Issues
All Issue Vol. 17 No. 2 (2023): June Vol. 17 No. 1 (2023): March Vol. 16 No. 2 (2022): December Vol. 16 No. 1 (2022): March Vol. 15 No. 3 (2021): September 2021 Vol. 15 No. 2 (2021): June 2021 Vol. 15 No. 1 (2021): March 2021 Vol. 15 No. 4 (2021): December Vol. 14 No. 4 (2020): December 2020 Vol. 14 No. 3 (2020): September 2020 Vol. 14 No. 2 (2020): June 2020 Vol. 14 No. 1 (2020): March 2020 Vol. 13 No. 4 (2019): December 2019 Vol. 13 No. 3 (2019): September 2019 Vol. 13 No. 2 (2019): June 2019 Vol. 13 No. 1 (2019): March 2019 Vol. 12 No. 3 (2018): September 2018 Vol. 12 No. 2 (2018): June 2018 Vol. 12 No. 1 (2018): March 2018 Vol. 11 No. 4 (2017): December 2017 Vol. 11 No. 3 (2017): September 2017 Vol. 11 No. 2 (2017): Juni 2017 Vol. 11 No. 1 (2017): March 2017 Vol. 10 No. 4 (2016): December 2016 Vol. 10 No. 3 (2016): September 2016 Vol. 10 No. 2 (2016): June 2016 Vol. 10 No. 1 (2016): March 2016 Vol. 9 No. 4 (2015): December 2015 Vol. 9 No. 3 (2015): September 2015 Vol. 9 No. 2 (2015): June 2015 Vol. 9 No. 1 (2015): March 2015 Vol. 8 No. 4 (2014): December 2014 Vol. 8 No. 3 (2014): September 2014 Vol. 8 No. 2 (2014): June 2014 Vol. 8 No. 1 (2014): March 2014 Vol. 7 No. 4 (2013): November 2013 Vol. 7 No. 3 (2013): September 2013 Vol. 7 No. 2 (2013): June 2013 Vol. 7 No. 1 (2013): March 2013 Vol. 6 No. 4 (2012): December 2012 Vol. 6 No. 3 (2012): September 2012 Vol. 6 No. 2 (2012): June 2012 Vol. 6 No. 1 (2012): March 2012 Vol. 5 No. 4 (2011): December 2011 Vol. 5 No. 3 (2011): September 2011 Vol. 5 No. 2 (2011): June 2011 Vol. 5 No. 1 (2011): March 2011 Vol. 4 No. 3 (2010): December 2010 Vol. 4 No. 2 (2010): August 2010 Vol. 4 No. 1 (2010): April 2010 Vol. 3 No. 3 (2009): December 2009 Vol. 3 No. 2 (2009): August 2009 Vol. 3 No. 1 (2009): April 2009 Vol. 2 No. 3 (2008): December 2008 Vol. 2 No. 2 (2008): August 2008 Vol. 2 No. 1 (2008): April 2008 Vol. 1 No. 3 (2007): December 2007 Vol. 1 No. 2 (2007): August 2007 Vol. 1 No. 1 (2007): April 2007 More Issue