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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Biospecies METAMORFOSA Journal of Biological Sciences Reaktor Bioma : Berkala Ilmiah Biologi Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Bahan Alam Terbarukan Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi Journal of Degraded and Mining Lands Management Indonesian Journal of Biotechnology Jurnal Agro Planta Tropika BIO-SITE |BIOLOGI Sains Terapan Majalah Obat Tradisional Journal of Natural Sciences and Mathematics Research Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology Omni-Akuatika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Agrotek Tropika Jurnal Riset Biologi dan Aplikasinya Berkala Ilmiah Biologi International Journal of Oil Palm
Rina Sri Kasiamdari
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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DAYA ANTIMIKROBA METABOLIT BIOAKTIF JAMUR SHIITAKE (Lentinula edodes (Berk.) Pegler) YANG DIKULTUR PADA TIGA JENIS MEDIUM FERMENTASI Ekowati, Nuraeni; Kasiamdari, Rina Sri; Pusposendjojo, Nursamsi; Soegihardjo, C.J.
Majalah Obat Tradisional Vol 16, No 3 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.742 KB) | DOI: 10.14499/mot-TradMedJ16iss3pp%p

Abstract

Jamur shiitake (Lentinula edodes (Berk.) Pegler) merupakan salah satu jamur yang berpotensi sebagai jamur pangan dan bahan obat (edible and medicinal mushroom). Kultivasi pada medium cair dengan proses fermentasi telah dikembangkan selama beberapa tahun terakhir ini. Empat isolat L. edodes (isolat asal Malang, Cianjur, Lembang dan Yogyakarta) dikultur pada tiga jenis medium fermentasi yaitu (KM: Kauffman Medium; GYMT: Glucose, Yeast extract,  Malt extract, Thiamin; YEMR: Yeast extract,  Malt extract,  Rice bran). Metabolit bioaktif yang diekstrak dari biomassa miselium dan filtrat kultur hasil fermentasi diuji menggunakan mikroba patogen Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218, Candida albicans ATCC 10231,dan  Trichophyton mentagrophytes. Data diameter zona  hambat dianalisis menggunakan analisis ragam, dan dilanjutkan dengan uji Duncan pada tingkat kesalahan 5 %. Hasil penelitian menunjukkan bahwa senyawa bioaktif dalam filtrat kultur maupun biomassa miselium L.edodes yang diekstrak menggunakan kloroform, etil asetat dan air, mampu menghambat S. aureus, E.coli dan C. albicans tetapi tidak mampu menghambat T.mentagrophytes. Isolat L.edodes asal Lembang dan Yogyakarta yang dikultur pada medium Kauffman memberikan hasil zona hambat terbaik (24,97-31,14 mm). Mikroba uji yang paling sensitif terhadap senyawa bioaktif dari L. edodes adalah C. albicans diikuti oleh E.coli, S. aureus dan T. mentagrophytes. Metabolit bioaktif dari L. edodes berpotensi menghambat mikroba patogen dari kelompok bakteri dan khamir tetapi tidak menghambat jamur.  
Genetic Relatedness among Duku, Kokosan, and Pisitan in Indonesia Based on Random Amplified Polymorphic DNA Markers Hanum, Laila; Kasiamdari, Rina Sri; ., Santosa; ., Rugayah
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

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Abstract

Genetic relatedness among duku, kokosan, and pisitan from Indonesia were investigated using random amplified polymorphic DNA (RAPD) markers. Eleven primers (OPA-01, OPA-02, OPA-10, OPB-07, OPB-11, OPB-12, OPB-15, OPT-16, OPU-14, OPU-19, and OPU-20) were used for amplification and yielded a total of 174 DNA bands, of which 167 were polymorphic. Primer OPA-10, OPB-11, OPB-12, OPB-15, and OPU-19 produced all of the polymorphic DNA bands. The size of the amplified DNA fragments ranged from 41-1546 bp. The dendrogram separated into two clusters at a genetic similarity coefficient of 0.76. The cluster 1 consisted of subclusters duku and several pisitan (pisitan OKI, pisitan Sleman, pisitan Hatu, pisitan Punggur, and pisitan Tanjung), and cluster 2 consisted of subclusters kokosan and pisitan. In the kokosan subclusters, including duku Drendan. Dendrogram supported the determination of taxonomic status of duku, kokosan, and pisitan as one species, namely Lansium domesticum Corr. and its divided into two groups, namely L. domesticum ’duku group’ and L. domesticum ’pisitan-kokosan group’. Thus, RAPD analysis was useful tool for determining the genetic variation and the genetics relatedness among duku, kokosan, and pisitan in Indonesia.Key words: duku, kokosan, pisitan/langsat, genetic relatedness, RAPD
Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon Daryono, Budi Setiadi; Aristya, Ganies Riza; Kasiamdari, Rina Sri
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

A random amplified polymorphic DNA (RAPD) marker linked to powdery mildew resistance gene (Pm-I) in melon PI 371795 was reported. However, the RAPD marker has problem in scoring. To detect powdery mildew resistance gene (Pm-I) in melon accurately, the RAPD marker was cloned and sequenced to design sequence characterized amplified region (SCAR) markers. SCAPMAR5 marker derived from pUBC411 primer yielded a single DNA band at 1061 bp. Segregation of SCAPMAR5 marker in bulk of F2 plants demonstrated that the marker was co-segregated with RAPD marker from which the SCAR marker was originated. Moreover, results of SCAR analysis in diverse melons showed SCAPMAR5 primers obtained a single 1061 bp linked to Pm-I in resistant melon PI 371795 and PMAR5. On the other hand, SCAPMAR5 failed to detect Pm-I in susceptible melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to Pm-I evaluated by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers.   Key words: Cucumis melo L., Pm-I, RAPD, SCAPMAR5
The Phylogenetic Relationship Among Varieties of Lansium domesticum Correa Based on ITS rDNA Sequences Hanum, Laila; Kasiamdari, Rina Sri; ., Santosa; ., Rugayah
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

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Abstract

Lansium domesticum Corr. with vernacular name in Indonesian duku has been reported containingtherapeutic bioactive compounds, and some of these compounds shown to be potent antitumor, anticancer,antimalaria, antimelanogenesis, antibacteria, and antimutagenic activities. This plant is commonly known asduku, kokosan and langsat by the local community in Indonesia. The morphological appearance of all varieties isnearly the same, and identifi cation of the varieties is very diffi cult for growers. Variation of DNA sequences ofthe ITS (Internal transcribed spacer) region can be used as a molecular character to determine the phylogeneticrelationship of different varieties of L. domesticum. The aims of this study were to determine taxonomy status ofduku, kokosan, and langsat, also phylogenetic relationship among varieties of L. domesticum based on ITS rDNAsequencing. DNA was isolated from leaves of plant and then amplifi ed using F1 and R1 primers. Nucleotidesequences were identifi ed using Sequence Scanner Software Programm version 1.0, nucleotide sequences from18S, ITS1, 5.8S, ITS2 and 26S region, that has been mergered using EditSeq and SegMan in software Suite forSequence Analysis DNASTAR Lasergene DM version 3.0.25. The results of study showed that DNA fragmentsranging in size from 782-810 bp. Different pattern of DNA fragments indicated polymorphism among duku,kokosan, and langsat. Based on the results of the ITS rDNA sequencing and phylogenetic tree analysis. Itwas determined that Lansium and Aglaia are a separated genus with the similarity index value of 0.98. Duku,kokosan and langsat were divided into two cluster, namely cluster kokosan-langsat and cluster duku with thesimilarity index value of 0.996.Keywords : Phylogenetic relationship, ITS region, L. domesticum, duku, kokosan, langsat
Sitotoksisitas Ekstrak Aspergillus fumigatus dari Daun Mekai (Albertisia papuana Becc.) terhadap Sel Kanker Payudara T47D dan MCF-7 Maritsa, Hasnaul; Moeljopawiro, Soekarti; Kasiamdari, Rina Sri
BIO-SITE |BIOLOGI Sains Terapan Vol 1, No 01 (2015): Bio-Site
Publisher : BIO-SITE |BIOLOGI Sains Terapan

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Abstract

The previous studies showed that the Albertisia papuana Becc. root have cytotoxicity on breast cancer. The A. papuana root toxicity on breast cancer could not only by plant secondary metabolism, but may be also by secondary metabolism of endophytes. Aspergillus fumigatus is one of endophytes that have anticancer agent. Endophytes can be distributed dynamically in whole of plant organ, one of them are leaves. Therefore the objective of this studies were to know the presence of A. fumigatus in A. papuana leaves, and the cytotoxicity of their secondary metabolism on breast cancer cells. The sample of A. papuana were collected from Botanical Zoo of Bogor, while T47D and MCF-7 cell lines were obtained of Tropical Medicine’s Faculty, UGM. Isolation of endophytes was done by growing leaves extract on water agar 2 % medium. Secondary metabolism was extracted from fermented broth using in ethyl acetat and n-butanol. The cytotoxicity was perform by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The result showed that A. fumigatus assosiated with A. papuana leaves. Ethyl acetat extract from fermented A. fumigatus both on T47D and MCF-7 cell lines had lower (IC50. 50, 444 µg/ml and 59 µg/ml) than n-butanol (IC50. 103, 398 µg/ml and 127,188 µg/ml. It could be said that A. fumigatus from A. papuana leaves could induce cytotoxicity on T47D and MCF-7 breast cancer cells.   Keywords: cancer, Aspergillus fumigatus, secondary metabolism, Albertisia papuana Becc., cytotoxiciy
KINERJA ENZIM GANDA PADA PRETREATMENT MIKROALGA UNTUK PRODUKSI BIOETANOL Padil, Padil; Syamsiah, Siti; Hidayat, Muslikhin; Kasiamdari, Rina Sri
Jurnal Bahan Alam Terbarukan Vol 5, No 2 (2016): December 2016 [Nationally Accredited]
Publisher : Semarang State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v5i2.7564

Abstract

The use of biomass of microalgae as a feedstock to produce bioethanol is very promising, it is caused by a large amount of carbohydrates contained in microalgae physiology cell. The main obstacle of enzymatic hydrolysis in order to produce bioethanol is the bound starch granules in a rigid cell wall. Therefore, pre-treatment steps needed to remove and convert complex carbohydrates into simple sugars before the fermentation process. Tetraselmis Chuii microalgae species are green microalgae (Chlorophyta) in which the cell wall containing cellulose and hemicellulose as the main constituent, therefore, this study observe the effect of the use of cellulase enzymes and xylanase as a strategy to open up the cell walls of microalgae. Another investigated parameter is the enzyme concentration, temperature, pH, and methods of use of enzymes. The results showed that the highest yield of glucose obtained was 31.912% (w / w) and is achieved under the conditions of a temperature of 45oC, pH of 4.5, the amount of biomass of microalgae as 5 g/L, the concentration of cellulase enzymes and xilanase 30% (w / w) at 40 minute at mechanism using cellulase and xylanase enzymes simultaneously.
KINERJA ENZIM GANDA PADA PRETREATMENT MIKROALGA UNTUK PRODUKSI BIOETANOL Padil, Padil; Syamsiah, Siti; Hidayat, Muslikhin; Kasiamdari, Rina Sri
Jurnal Bahan Alam Terbarukan Vol 5, No 2 (2016): December 2016 [Nationally Accredited]
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbat.v5i2.7564

Abstract

The use of biomass of microalgae as a feedstock to produce bioethanol is very promising, it is caused by a large amount of carbohydrates contained in microalgae physiology cell. The main obstacle of enzymatic hydrolysis in order to produce bioethanol is the bound starch granules in a rigid cell wall. Therefore, pre-treatment steps needed to remove and convert complex carbohydrates into simple sugars before the fermentation process. Tetraselmis Chuii microalgae species are green microalgae (Chlorophyta) in which the cell wall containing cellulose and hemicellulose as the main constituent, therefore, this study observe the effect of the use of cellulase enzymes and xylanase as a strategy to open up the cell walls of microalgae. Another investigated parameter is the enzyme concentration, temperature, pH, and methods of use of enzymes. The results showed that the highest yield of glucose obtained was 31.912% (w / w) and is achieved under the conditions of a temperature of 45oC, pH of 4.5, the amount of biomass of microalgae as 5 g/L, the concentration of cellulase enzymes and xilanase 30% (w / w) at 40 minute at mechanism using cellulase and xylanase enzymes simultaneously.
Phylogenetic Relationships of Nine Cultivars of Strawberries (Fragaria spp.) Based on Anatomical and Morphological Characters Kasiamdari, Rina Sri; Aristya, Ganies Riza; Inayati, Evi
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 5, No 2 (2017)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2017.072.116-126

Abstract

Strawberry cultivation in Indonesia is centred at Citrus and Subtropical Fruit Research Institute, which collects different cultivars of Fragaria spp. Information on classification based on phenotypic characters of Fragaria spp. has not been studied as a whole. The purpose of the present study was to identify and determine phylogenic relationships of nine cultivars of strawberries based on the anatomical and morphological characters. The present study identified morphologically nine strawberry cultivars by referring to the IPGRI (1986) and the UPOV (2012) and Hofer et al. (2012). Anatomical preparations of roots, stems and leaves were made by the embedding method. Phylogenetic relationships were determined using MVSP software with UPGMA algorithm through the Gower General Similarity Coefficient method. Subsequently, the principal components were analyzed using the Euclidian Biplots algorithm depicted in a Scatter plot. Results showed that there were 37 anatomical and morphological characters that play a role in the characterization of nine cultivars (25 synthetic characters and 12 diagnostics characters) where the shape of the fruit and type of attachment of the flowers were the main characters distinguishing among the nine cultivars studied. The phylogenetic relationship produced two clusters with 79% similarity index. The closely related cultivars were Festival and Rosa Linda with a similarity index of 94%, and Earlibrite and Aerut with a similarity index of 86%
Morphological Characteristics and Kinship Relationship of Mushroom Schizophyllum commune Fr. Kusrinah, Kusrinah; Kasiamdari, Rina Sri
Journal Of Natural Sciences And Mathematics Research Vol 1, No 2 (2015): Volume 1, Nomor 2, 2015
Publisher : Faculty of Science and Technology, State Islamic University Walisongo Central Java

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/jnsmr.2015.1.2.1620

Abstract

Schizophyllum commune is a mushroom from the division of Basidiomycota which abundance at rainy season on wood that have been moldered for its habitat. S. commune has been distributed widely at various types of wood habitat (jack fruit, bamboo, rubber, mango etc). Existence of this mushroom has not been known as well as any mushroom are able to be consumed/edible yet. In Java, the societies who have known it as consumption mushroom called it with local name jamur gigit. Jamur gigit is potential to become food material and based on organoleptic test indicates that jamur gigit is more delicious from oyster mushroom. The purpose of research is to investigate the morphology character of S. commune at various growth phases and to study the S. commune relationship in Java. The result of this research is expected can give information about morphology character of S. commune in each its growth phase and to understand S. commune relationship in Java. Sample for research has been collected in 3 areas in Java. The macroscopic morphology character and microscopic character of S. commune were observed. The character that had been obtained is used to compile description and be analyzed by cluster using SPSS 13.0 to study the kinship relationship. The result of research indicates that morphology character of S. commune in Java of fruit body when old and young phase was different, while the microscopic character almost same to the obtained sample. The result of analysis cluster indicated that at similar level of 38,1 % there were two clusters, first cluster was Sidoarjo a S. commune and S. commune Yogyakarta. The second cluster has member the Sidoarjo b S. commune, Malang S. commune, Tangerang S. commune, Semarang S. commune and Kuningan S. commune. © 2015 JNSMR UIN Walisongo. All rights reserved.
Variasi Genetik Berdasarkan Penanda Molekular Random Amplified Polymorphic DNA Pada Jamur Shiitake (Lentinula edodes) Ekowati , Nuraeni; Sri Kasiamdari , Rina; Pusposendjojo , Nursamsi; Soegihardjo , C.J.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.867 KB) | DOI: 10.24002/biota.v16i2.97

Abstract

Penelitian ini bertujuan mengetahui variasi genetik jamur Lentinula edodes asal Malang, Cianjur, Lembang, dan Yogyakarta serta mendapatkan primer terseleksi untuk identifikasi L. edodes secara molekular. Penelitian dilakukan di Laboratorium Genetika, Fakultas Biologi, Universitas Gadjah Mada, Yogyakarta. Tahapan penelitian terdiri atas ekstraksi DNA dari miselium empat isolat L. edodes dan satu sampel outgroup (Pleurotus ostreatus), amplifikasi DNA dengan teknik PCR-RAPD menggunakan delapan jenis primer (OPA 1, OPA 2, OPA 3, OPA 4, OPA 7, OPA 8, OPA 9 dan OPA 10), elektroforesis menggunakan gel agarosa dan pengamatan pita DNA dengan UV transluminator. Data pita DNA dianalisis dengan software NTSYSpc21 untuk menentukan tingkat similaritas, jarak genetik dan untuk mengkonstruksi dendrogram berdasarkan metode UPGMA. Hasil penelitian menunjukkan bahwa semua primer yang digunakan dapat mengamplifikasi DNA sampel dan satu diantaranya (OPA 4) tidak dapat menunjukkan adanya polimorfisme pada keempat isolat. Ukuran fragmen DNA teramplifikasi berkisar antara 1291774 bp. Berdasarkan hasil penelitian dapat disimpulkan bahwa antara isolat L. edodes asal Malang, Cianjur, Lembang dan Yogyakarta terdapat variasi genetik dengan jarak genetik antara 7886%. Polimorfisme tertinggi (83,33%) diperoleh menggunakan primer OPA 2.
Co-Authors ', Padil AA Sudharmawan, AA Achmadi Priyatmojo Adiana Nayogyani Ali Djamhuri Ali, Nur Ayu Aisyah Ana Susianti Andang Syaifudin Ani Widiastuti, Ani Auli, Nisa Raudatul Ayundai, Melin Ayundai, Melin BUDI SETIADI DARYONO BUDI SETIADI DARYONO C. J. Soegihardjo C.J. Soegihardjo C.J. Soegihardjo C.J. SOEGIHARDJO Chasanah, Laila Uswatun Dhia Salsabila Hakim Dian Fitriarni Ekowati , Nuraeni Elzahra Nadya Putri Endang Sutariningsih Soetarto ENDANG SUTARININGSIH SOETARTO Erni Martani Erni Martani Febriani, Anya Via Ganies Riza Aristya Hasnaul Maritsa I Gusti Wayan Murjana Yasa Inayati, Evi Indra Sukmawati Junita Hardini Khaterine K Kristamtini Kristamtini, Kristamtini Kusrinah Kusrinah Kusrinah Kusrinah Laila Hanum Maulin Nafisa Muazam, Arif Muslikhin Hidayat Nita Aminasih Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati, Nuraeni Nursamsi Pusposendjojo Nursamsi Pusposendjojo Nursamsi Pusposendjojo Nursamsi Pusposendjojo, Nursamsi Nursela, Dewi Padil, Padil Purnomo Purnomo Pusposendjojo , Nursamsi Putranto, Dwiyandito Ikhsan Putri, Elzahra Nadya Putri, Fauzana Putri, Fauzana R. Rugayah Ratna Stia Dewi Ratna Stia Dewi Reine Suci Wulandari Rejeki Siti Ferniah Retno Peni Sancayaningsih Retno Peni Sancayaningsih RETNO PENI SANCAYANINGSIH Ristiyani Khofifa Putri ROSA SURYANTINI Rugayah Rugayah S. Santosa Santosa . Santosa Santosa Santosa Santosa Sari, Noorkomala Setyorini Widyayanti, Setyorini Singgih Tri Wardana Siti Syamsiah Siti Syamsiah Soegihardjo , C.J. Soekarti Moeljopawiro Soekarti Moeljopawiro, Soekarti Suharno Suharno Susiana Purwantisari Sutikno S Syamsiah, Siti Widiastuti, Ani Yekti Asih Purwestri Yudhistira Nugraha